Elektroanatomische Rekonstruktion akzessorischer atrioventrikulärer Leitungsbahnen

Diese Arbeit umfasst Ergebnisse einer klinischen Untersuchung zur elektroanatomischen Rekonstruktion des Mitralklappenannulus bei Pat. mit akzessorischen Leitungsbahnen. Die Untersuchung basiert auf dem Cartosystem, einem Mappingsystem zur Erstellung eines 3D anatomisch korrektem Modell des Herzens mit farbkodierter Darstellung der Aktivierungszeit der unterschiedlichen Myokardregionen. Erstellt wurden Rekonstruktionen der ventrikulären Insertion linksgelegener akzessorischer Leitungsbahnen. Durch die computergesteuerte Annotierung der Elektrogramme konnte die ventrikuläre Insertion der Leitungsbahn exakt lokalisiert und effektiv abladiert werden. Der Vergleich des Oberflächen-EKG bei maximaler Präexzitation und des mit dem Cartosystem erstellten elektroanatomischen ventrikulären Insertionsbereichs ermöglichte eine Zuordnung der anatomischen Lage der akzessorischen Bahn anhand der charakteristischen EKG-Morphologie. Vier unterschiedliche Lokalisationen konnten erarbeitet werden

Regional Variation in the Density of Essential Genes in Mice

In most species, and particularly in vertebrates, the percentage of genes absolutely required for survival, the essential genes, has not been estimated. To obtain this estimation, we used the mouse as an experimental model to carry out high-efficiency N-ethyl-N-nitrosourea (ENU) mutagenesis screens in two balancer chromosome regions, and compared our results to a third previously published screen. The number of essential genes in each region was predicted based on allele frequencies. We determined that the density of essential genes differs by up to an order of magnitude among genomic regions. This indicates that extrapolating from regional estimates to genome-wide estimates of essential genes has a huge variance. A particularly high density of essential genes on mouse Chromosome 11 coincides with a high degree of regional linkage conservation, providing a possible causal explanation for the density variation. This is the first demonstration of regional variation in essential gene density in the mouse genome

Neuroendocrine Regulation of Metabolism

Given the current environment in most developed countries, it is a challenge to maintain a good balance between calories consumed and calories burned, although maintenance of metabolic balance is key to good health. Therefore, understanding how metabolic regulation is achieved and how the dysregulation of metabolism affects health is an area of intense research. Most studies focus on the hypothalamus, which is a brain area that acts as a key regulator of metabolism. Among the nuclei that comprise the hypothalamus, the arcuate nucleus is one of the major mediators in the regulation of food intake. The regulation of energy balance is also a key factor ensuring the maintenance of any species as a result of the dependence of reproduction on energy stores. Adequate levels of energy reserves are necessary for the proper functioning of the hypothalamic-pituitary-gonadal axis. This review discusses valuable data presented in the 2015 edition of the International Workshop of Neuroendocrinology concerning the fundamental nature of the hormonal regulation of the hypothalamus and the impact on energy balance and reproduction.Instituto Multidisciplinario de Biología CelularConsejo Nacional de Investigaciones Científicas y Técnica

Implementing educational and systems-level changes to improve cancer screening rates among state employees in Missouri

As of 2022, only 51% of active eligible state employees in Missouri have been screened for colorectal cancer and 67% for breast cancer, despite having state-sponsored health insurance. In fall 2020, the Missouri Department of Health and Senior Services Comprehensive Cancer Program partnered with the Missouri Cancer Consortium to create a strategy to improve cancer screening rates among state employees. The project was designed to include 3 phases: 1) a colorectal cancer education phase, 2) an expanded education phase that included additional cancers, and 3) a proposed intervention phase that will include screening events. In the first phase, in 2020, colorectal cancer educational materials were sent to all state employees. In the second phase, in 2022, educational resources were expanded to include additional cancers and screening tools. In both initiatives, educational materials and information on current screening recommendations were distributed to approximately 40,000 state employees. A database of screening rates was developed to monitor screening rates and challenge state employees to complete screenings. Evidence-informed interventions were implemented with a focus on health equity. We used a regional approach to identify geographic areas with the greatest need. These efforts will support the next phase of the project, which involves planning breast and colorectal cancer screening events. Policy changes will be encouraged to remove systems-level barriers that discourage employees from being screened for cancer. Recommended tools and strategies can be adopted by similar organizations with complex, multitier employee structures

Aumento de durabilidade de argamassas para pocilgas com utilização de sílica ativa e nanossílica.

RESUMO: Os materiais cimentícios são os mais utilizados em construções agrícolas, no entanto, não há uma recomendação estabelecida para concretos e argamassas em ambientes agrícolas no Brasil, e mais estudos ainda são necessários. Contudo, os dejetos suínos podem causar desgaste e danos às estruturas cimentícias de pocilgas, diminuindo sua durabilidade. Neste contexto, este estudo avaliou o comportamento de argamassas com sílica ativa e nanossílica a um ataque ácido, simulando os efeitos dos ácidos presentes nos dejetos suínos. Foi investigada a utilização de 15% de substituição do cimento por sílica ativa em argamassa, bem como a incorporação de nanossílica como aditivo. As amostras foram submetidas a ciclos de imersão em solução ácida e imersão em água com cal. A perda de massa foi medida ao longo dos ciclos e as resistências à compressão aos 28 dias e após os ciclos de ataque ácido também foram determinadas. Os resultados indicaram que o uso combinado de sílica ativa a 15% e nanossílica aumentou a resistência à compressão das argamassas em 20%. Após o ataque ácido, as argamassas com 15% de substituição de cimento por sílica ativa e uso de nanossílica apresentaram menor perda de resistência à compressão e menor perda de massa do que as argamassas de referência. ABSTRACT: Cementitious materials are the most used ones in agricultural constructions, however there is no established recommendation for concretes and mortars for agricultural environment in Brazil, and further studies are still necessary. However, swine manure can cause loss of strength and damage to cementitious structures in pig houses, decreasing their durability. In this context, this study evaluated the behavior of mortars with silica fume and nanosilica to an acid attack, simulating the effects of acids present in swine manure. The use of 15% replacement of cement by silica fume in mortar was investigated, as well as the incorporation of nanosilica as an additive. The samples were submitted to immersion cycles in acidic solution and immersion in saturated limewater. The mass loss was measured along the cycles and the compressive strengths at 28 days and after the acid attack were also determined. The results indicated that the combined use of silica fume at 15% and nanosilica increased the compressive strength of mortars by 20%. After the acid attack, the mortars with 15% replacement of cement by silica fume and the use of nanosilica showed less loss of compressive strength and less mass loss than the reference mortars

A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus

Neurogenesis in the dentate gyrus continues into adulthood, yet little is known about the function of newly born neurons or how they integrate into an existing network of mature neurons. We made transgenic mice that selectively and transiently express enhanced green fluorescent protein (EGFP) in newly born granule cells of the dentate gyrus under the transcriptional control of proopiomelanocortin (POMC) genomic sequences. Analysis of transgenic pedigrees with truncation or deletion mutations indicated that EGFP expression in the dentate gyrus required cryptic POMC promoter regions dispensable for arcuate hypothalamic or pituitary expression. Unlike arcuate neurons, dentate granule cells did not express the endogenous POMC gene. EGFP-positive neurons had immature properties, including short spineless dendrites and small action potentials. Colocalization with bromodeoxyuridine indicated that EGFP-labeled granule cells were 2 weeks postmitotic. EGFP-labeled cells expressed markers for immature granule cells but not the glial marker GFAP. The number of EGFP-labeled neurons declined with age and increased with exercise, paralleling neurogenesis. Our results indicate that POMC-EGFP marks immature granule cells and that adult-generated granule cells integrate quite slowly into the hippocampal circuitry

Power tests of a string of magnets comprising a full cell of the Superconducting Super Collider

In this paper we describe the operation and testing of a string of magnets comprising a full cell of the Superconducting Super Collider (SSC). The full cell configuration composed of ten dipoles, two quadrupoles, and three spool pieces is the longest SSC magnet string ever tested. Although the tests of the full cell were undertaken after the SSC project was marked for termination, their completion was deemed necessary and useful to future efforts at other accelerator laboratories utilizing Superconducting magnets. The focus of this work is on the electrical and cryogenic performance of the string components and the quench protection system with an emphasis on solving some of the questions concerning electrical performance raised during the previous two experimental runs involving a half cell configuration

Threats and Supports to Female Students’ Math Beliefs and Achievement

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/149563/1/jora12384_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/149563/2/jora12384.pd

Integrating the Totality of Food and Nutrition Evidence for Public Health Decision Making and Communication

The interpretation and integration of epidemiological studies detecting weak associations (RR < 2) with data from other study designs (e.g., animal models and human intervention trials) is both challenging and vital for making science-based dietary recommendations in the nutrition and food safety communities. The 2008 ILSI North America “Decision-Making for Recommendations and Communication Based on Totality of Food-Related Research” workshop provided an overview of epidemiological methods, and case-study examples of how weak associations have been incorporated into decision making for nutritional recommendations. Based on the workshop presentations and dialogue among the participants, three clear strategies were provided for the use of weak associations in informing nutritional recommendations for optimal health. First, enable more effective integration of data from all sources through the use of genetic and nutritional biomarkers; second, minimize the risk of bias and confounding through the adoption of rigorous quality-control standards, greater emphasis on the replication of study results, and better integration of results from independent studies, perhaps using adaptive study designs and Bayesian meta-analysis methods; and third, emphasize more effective and truthful communication to the public about the evolving understanding of the often complex relationship between nutrition, lifestyle, and optimal health