756 research outputs found
Barrier properties of films of pea starch associated with xanthan gum and glycerol
O objetivo do trabalho foi avaliar as propriedades de barreira e a solubilidade de biofilmes obtidos a partir de amido de ervilha de alto teor de amilose em associação à goma xantana e glicerol. Soluções filmogênicas (SF) com diferentes teores de amido de ervilha (3, 4 e 5%), goma xantana (0, 0,05 e 0,1%) e glicerol (proporção glicerol-amido de 1:5 P/P) foram estudadas. As SF foram obtidas por ebulição (5 minutos), seguida de autoclavagem por 1 hora a 120 ºC e os filmes foram preparados por casting. O aumento da concentração de amido e de glicerol na composição causou aumento da espessura e da solubilidade dos filmes em água. O plastificante gerou ainda elevação dos coeficientes de permeabilidade ao vapor d'água e ao oxigênio. O aumento da concentração da goma xantana não interferiu nas propriedades estudadas. Os biofilmes obtidos a partir de amido de ervilha verde, associado ou não à goma xantana e glicerol, se comparados com filmes de amido de ervilha amarelas e outras fontes de amido, apresentaram boa barreira ao oxigênio e ao vapor d'água e baixa solubilidade em água.The aim of this work was to evaluate the barrier properties and solubility of biofilms made from wrinkled pea starch with high amylose content in association with xanthan gum and glycerol. Filmogenic solution (FS) with different levels of pea starch (3, 4 and 5%), xanthan gum (0, 0.05 and 0.1%) and glycerol (glycerol-starch 1:5 W/W) were tested. FS was obtained by boiling (5 minutes), autoclaving for 1 hour at 120 ºC and the films were prepared by casting. The increased concentration of starch and glycerol in the composition caused increases in thickness of the films and in their solubility in water. The plasticizer also generated higher coefficients of water vapor and oxygen permeabilities to water vapor and to oxygen. The increasing concentration of xanthan gum did not interfere in the properties studied. Biofilms produced with wrinkled pea starch, with or without xanthan gum and glycerol, showed better barrier to oxygen and water vapor and low solubility in water, in comparison with films of yellow pea starch and other starch sources.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP
lcc: an R package to estimate the concordance correlation, Pearson correlation and accuracy over time
Background and Objective: Observational studies and experiments in medicine,
pharmacology and agronomy are often concerned with assessing whether different
methods/raters produce similar values over the time when measuring a quantitative
variable. This article aims to describe the statistical package lcc, for are, that can
be used to estimate the extent of agreement between two (or more) methods over
the time, and illustrate the developed methodology using three real examples.
Methods: The longitudinal concordance correlation, longitudinal Pearson
correlation, and longitudinal accuracy functions can be estimated based on fixed
effects and variance components of the mixed-effects regression model. Inference is
made through bootstrap confidence intervals and diagnostic can be done via plots,
and statistical tests.
Results: The main features of the package are estimation and inference about the
extent of agreement using numerical and graphical summaries. Moreover, our
approach accommodates both balanced and unbalanced experimental designs or
observational studies, and allows for different within-group error structures, while
allowing for the inclusion of covariates in the linear predictor to control systematic
variations in the response. All examples show that our methodology is flexible and
can be applied to many different data types.
Conclusions: The lcc package, available on the CRAN repository, proved to be a
useful tool to describe the agreement between two or more methods over time,
allowing the detection of changes in the extent of agreement. The inclusion of
different structures for the variance-covariance matrices of random effects and
residuals makes the package flexible for working with different types of databases
Periodically kicked turbulence
Periodically kicked turbulence is theoretically analyzed within a mean field
theory. For large enough kicking strength A and kicking frequency f the
Reynolds number grows exponentially and then runs into some saturation. The
saturation level can be calculated analytically; different regimes can be
observed. For large enough Re we find the saturation level to be proportional
to A*f, but intermittency can modify this scaling law. We suggest an
experimental realization of periodically kicked turbulence to study the
different regimes we theoretically predict and thus to better understand the
effect of forcing on fully developed turbulence.Comment: 4 pages, 3 figures. Phys. Rev. E., in pres
Differences in Secure Messaging, Self-management, and Glycemic Control Between Rural and Urban Patients: Secondary Data Analysis
BACKGROUND: Rural patients with diabetes have difficulty accessing care and are at higher risk for poor diabetes management. Sustained use of patient portal features such as secure messaging (SM) can provide accessible support for diabetes self-management.
OBJECTIVE: This study explored whether rural patients\u27 self-management and glycemic control was associated with the use of SM.
METHODS: This secondary, cross-sectional, mixed methods analysis of 448 veterans with diabetes used stratified random sampling to recruit a diverse sample from the United States (rural vs urban and good vs poor glycemic control). Administrative, clinical, survey, and interview data were used to determine patients\u27 rurality, use of SM, diabetes self-management behaviors, and glycemic control. Moderated mediation analyses assessed these relationships.
RESULTS: The sample was 51% (n=229) rural and 49% (n=219) urban. Mean participant age was 66.4 years (SD 7.7 years). More frequent SM use was associated with better diabetes self-management (P=.007), which was associated with better glycemic control (P \u3c .001). Among rural patients, SM use was indirectly associated with better glycemic control through improved diabetes self-management (95% CI 0.004-0.927). These effects were not observed among urban veterans with diabetes (95% CI -1.039 to 0.056). Rural patients were significantly more likely than urban patients to have diabetes-related content in their secure messages (P=.01).
CONCLUSIONS: More frequent SM use is associated with engaging in diabetes self-management, which, in turn, is associated with better diabetes control. Among rural patients with diabetes, SM use is indirectly associated with better diabetes control. Frequent patient-team communication through SM about diabetes-related content may help rural patients with diabetes self-management, resulting in better glycemic control
Bottleneck effects in turbulence: Scaling phenomena in r- versus p-space
We (analytically) calculate the energy spectrum corresponding to various
experimental and numerical turbulence data analyzed by Benzi et al.. We find
two bottleneck phenomena: While the local scaling exponent of the
structure function decreases monotonically, the local scaling exponent
of the corresponding spectrum has a minimum of
at and a maximum
of at . A physical
argument starting from the constant energy flux in p--space reveals the general
mechanism underlying the energy pileups at both ends of the p--space scaling
range. In the case studied here, they are induced by viscous dissipation and
the reduced spectral strength on the scale of the system size, respectively.Comment: 9 pages, 3figures on reques
Patients with paroxysmal nocturnal hemoglobinuria have a high frequency of peripheral-blood T cells expressing activating isoforms of inhibiting superfamily receptors
Patients with paroxysmal nocturnal hemoglobinuria (PNH) have a large clonal population of blood cells deriving from hematopoietic stem cells (HSCs) deficient in glycosylphosphatidylinositol (GPI)-anchored surface molecules. A current model postulates that PNH arises through negative selection against normal HSCs exerted by autoreactive T cells, whereas PNH HSCs escape damage. We have investigated the inhibitory receptor superfamily (IRS) system in 13 patients with PNH. We found a slight increase in the proportion of T cells expressing IRS. In contrast to what applies to healthy donors, the engagement of IRS molecules on T cells from patients with PNH elicited a powerful cytolytic activity in a redirected killing assay, indicating that these IRSs belong to the activating type. This was confirmed by clonal analysis: 50% of IRS+ T-cell clones in patients with PNH were of the activating type, while only 5% were of the activating type in healthy donors. Moreover, the ligation of IRS induces (1) production of tumor necrosis factor alpha (TNF-alpha) and interferon gamma (IFN-gamma) and (2) brisk cytolytic activity against cells bearing appropriate IRS counter-ligands. In addition, these IRS+ T cells show natural killer (NK)-like cytolytic activity to which GPI- cells were less sensitive than GPI+ cells. Thus, T cells with NK-like features, expressing the activating isoforms of IRS, may include effector cells involved in the pathogenesis of PNH
Transcriptional Characterization of a Widely-Used Grapevine Rootstock Genotype under Different Iron-Limited Conditions
Iron chlorosis is a serious deficiency that affects orchards and vineyards reducing quality and yield production. Chlorotic plants show abnormal photosynthesis and yellowing shoots. In grapevine iron uptake and homeostasis are most likely controlled by a mechanism known as "Strategy I," characteristic of non-graminaceous plants and based on a system of soil acidification, iron reduction and transporter-mediated uptake. Nowadays, grafting of varieties of economic interest on tolerant rootstocks is widely used practice against many biotic and abiotic stresses. Nevertheless, many interspecific rootstocks, and in particular those obtained by crossing exclusively non-vinifera genotypes, can show limited nutrient uptake and transport, in particular for what concerns iron. In the present study, 101.14, a commonly used rootstock characterized by susceptibility to iron chlorosis was subjected to both Fe-absence and Fe-limiting conditions. Grapevine plantlets were grown in control, Fe-deprived, and bicarbonate-supplemented hydroponic solutions. Whole transcriptome analyses, via mRNA-Seq, were performed on root apices of stressed and unstressed plants. Analysis of differentially expressed genes (DEGs) confirmed that Strategy I is the mechanism responsible for iron uptake in grapevine, since many orthologs genes to the Arabidopsis "ferrome" were differentially regulated in stressed plant. Molecular differences in the plant responses to Fe absence and presence of bicarbonate were also identified indicating the two treatments are able to induce response-mechanisms only partially overlapping. Finally, we measured the expression of a subset of genes differentially expressed in 101.14 (such as IRT1, FERRITIN1, bHLH38/39) or known to be fundamental in the "strategy I" mechanism (AHA2 and FRO2) also in a tolerant rootstock (M1) finding important differences which could be responsible for the different degrees of tolerance observed
Streamer Propagation as a Pattern Formation Problem: Planar Fronts
Streamers often constitute the first stage of dielectric breakdown in strong
electric fields: a nonlinear ionization wave transforms a non-ionized medium
into a weakly ionized nonequilibrium plasma. New understanding of this old
phenomenon can be gained through modern concepts of (interfacial) pattern
formation. As a first step towards an effective interface description, we
determine the front width, solve the selection problem for planar fronts and
calculate their properties. Our results are in good agreement with many
features of recent three-dimensional numerical simulations.Comment: 4 pages, revtex, 3 ps file
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