Nutritional and health status of woolly monkeys

Woolly monkeys (Lagothrix lagotricha and L. flavicauda) are threatened species in the wild and in captivity. Numerous zoological institutions have historically kept Lagothrix lagotricha spp., but only a few of them have succeeded in breeding populations. Therefore the majority of institutions that formerly kept Lagothrix lagotricha are no longer able or willing to do so. Captive populations of the species have frequent health problems, most significantly hypertension and related disorders. Researchers have conducted free-ranging dietary and behavior studies with respect to woolly monkeys, but have established no concrete link between diet or nutrients and captive health problems. The available literature we discuss indicates that researchers need to examine the link further. In addition, it is critical to the survival of the primates to be able to keep breeding populations in captivity owing to increasing natural pressures such as deforestation and hunting. Therefore, better understanding of the captive and free-ranging behavior and health parameters of the species is vital to ensure their survival and to maintain forest health and diversity. Researchers need to conduct large-scale research studies comparing the health and complete diet of individuals in the wild and captivity to resolve health problems facing the species in captivity

Facilitated engraftment of human hematopoietic cells in severe combined immunodeficient mice following a single injection of Cl²MDP liposomes

Transplantation of normal and malignant human hematopoietic cells into severe combined immunodeficient (SCID) mice allows for evaluation of long-term growth abilities of these cells and provides a preclinical model for therapeutic interventions. However, large numbers of cells are required for successful engraftment in preirradiated mice due to residual graft resistance, that may be mediated by cells from the mononuclear phagocytic system. Intravenous (i.v.) injection of liposomes containing dichloromethylene diphosphonate (Cl2MDP) may eliminate mouse macrophages in spleen and liver. In this study outgrowth of acute myeloid leukemia (AML) cells and umbilical cord blood (UCB) cells in SCID mice conditioned with a single i.v. injection of Cl2MDP liposomes in addition to sublethal total body irradiation (TBI) was compared to outgrowth of these cells in SCID mice that had received TBI alone. A two- to 10-fold increase in outgrowth of AML cells was observed in four cases of AML. Administration of 107 UCB cells reproducibly engrafted SCID mice that had been conditioned with Cl2MDP liposomes and TBI, whereas human cells were not detected in mice conditioned with TBI alone. As few as 2 x 104 purified CD34+ UCB cells engrafted in all mice treated with Cl2MDP liposomes. In SCID mice treated with macrophage depletion unexpected graft failures were not observed. Histological examination of the spleen showed that TBI and Cl2MDP liposomes i.v. resulted in a transient elimination of all macrophage subsets in the spleen, whereas TBI had a minor effect. Cl2MDP liposomes were easy to use and their application was not associated with appreciable side-effects. Cl2MDP liposome pretreatment in combination with TBI allows for reproducible outgrowth of high numbers of human hematopoietic cells in SCID mice

Patient handover in a European border region: Cross-sectional survey study among healthcare workers to explore the status quo, potential risks, and solutions

Introduction: While the popularity of international care is rising, the complexity of international care compromises patient safety. To identify risks and propose solutions to improve international care, this study explores experiences of healthcare workers with international handovers in a European border region.Methods: A cross-sectional survey design was used to reach out to 3000 healthcare workers, working for hospitals or emergency services in three neighboring countries in the Meuse-Rhine Euregion. In total, 846 healthcare workers completed the survey with 35 closed- and open-ended questions about experiences with international patient handover.Results: One-third of respondents had been involved in international handover in the previous month. The handovers occurred in planned and acute care settings and were supported by numerous, yet varying standardized procedures. Healthcare workers were trained for this in some, but not all settings. Respondents mentioned 408 risks and proposed 373 solutions, which were inductively analyzed. Six identified themes classify the level on which risks and accompanying solutions can be found: awareness, professional competencies, communication between professionals, loss of information, facilities and support, and organizational structure.Discussion: This study gives insight in international patient handovers in a European border region. Among the biggest risks experienced are procedural differences, sharing patient information, unfamiliarity with foreign healthcare systems, and not knowing roles and responsibilities of peers working across the border. Standardization of procedures, harmonization of systems, and the possibility for healthcare workers to get to know each other will contribute to reach common ground and move towards optimized and patient-safer cross-border care

Partial Flavor Symmetry Restoration for Chiral Staggered Fermions

We study the leading discretization errors for staggered fermions by first constructing the continuum effective Lagrangian including terms of O(a^2), and then constructing the corresponding effective chiral Lagrangian. The terms of O(a^2) in the continuum effective Lagrangian completely break the SU(4) flavor symmetry down to the discrete subgroup respected by the lattice theory. We find, however, that the O(a^2) terms in the potential of the chiral Lagrangian maintain an SO(4) subgroup of SU(4). It follows that the leading discretization errors in the pion masses are SO(4) symmetric, implying three degeneracies within the seven lattice irreducible representations. These predictions hold also for perturbatively improved versions of the action. These degeneracies are observed, to a surprising degree of accuracy, in existing data. We argue that the SO(4) symmetry does not extend to the masses and interactions of other hadrons (vector mesons, baryons, etc), nor to higher order in a^2. We show how it is possible that, for physical quark masses of O(a^2), the new SO(4) symmetry can be spontaneously broken, leading to a staggered analogue of the Aoki-phase of Wilson fermions. This does not, however, appear to happen for presently studied versions of the staggered action.Comment: 26 pages, 2 figures (using psfig). Version to appear in PRD (clarifications added to introduction and section 6; typos corrected; references updated

Особливості функціонування оцінних дієслівних лексем у публіцистичному дискурсі

Стаття присвячена дослідженню семантико-стилістичних особливостей оціночної дієслівної лексики в публіцистичному дискурсі, виділенню тематичних груп інгерентно та адгерентно оціночної дієслівної лексики, а також визначенню основних типів сенсибилізації оціночних дієслів-прагмем. Ключові слова: публіцистичний дискурс, дискурс, прагмема, іформема, дієслова інгерентної оцінки, адгерентно оціночні дієслова, дієслівна прагмема, сенсибілізація, лінгвістичні сенсибілізатори.Статья посвящена исследованию семантико-стилистических особенностей оценочной глагольной лексики в публицистическом дискурсе, выделению тематических групп ингерентно и адгерентно оценочной глагольной лексики, а также определению основных типов сенсибилизации оценочных глаголов-прагмем. Ключевые слова: публицистический дискурс, прагмема, информема, глаголы ингерентной оценки, адгерентно оценочные глаголы, глагольная прагмема, сенсибилизация, лингвистические сенсибилизаторы.The article is dedicated to study of the semantic-stylistic features of estimated verbal vocabulary in publicistic discourse, to allocation of thematic groups ingerentno and angerentno estimated verbal vocabulary, and also to definition of the basic types of a sensubulisation of estimated verbs-pragmem. Keywords: Publicistic discourse, pragmems, informem, the ingerentno estimated verbs, the angerentno estimated verbs, verbal pragmems, a sensubulisation, linguistic sensubulisator

Recreating tumour complexity in a dish: Organoid models to study liver cancer cells and their extracellular environment

Primary liver cancer, consisting predominantly of hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA), remains one of the most lethal malignancies worldwide. This high malignancy is related to the complex and dynamic interactions between tumour cells, stromal cells and the extracellular environment. Novel in vitro models that can recapitulate the tumour are essential in increasing our understanding of liver cancer. Herein, primary liver cancer-derived organoids have opened up new avenues due to their patient-specificity, self-organizing ability and potential recapitulation of many of the tumour properties. Organoids are solely of epithelial origin, but incorporation into co-culture models can enable the investigation of the cellular component of the tumour microenvironment. However, the extracellular component also plays a vital role in cancer progression and representation is lacking within current in vitro models. In this review, organoid technology is discussed in the context of liver cancer models through comparisons to other cell culture systems. In addition, the role of the tumour extracellular environment in primary live

One Spin Trace Formalism for BK B_K

It has been known for some time that there are two methods to calculate $B_K$ with staggered fermions: one is the two spin trace formalism and the other is the one spin trace formalism. Until now, the two spin trace formalism has been exclusively used for weak matrix element calculations with staggered fermions. Here, the one spin trace formalism to calculate $B_K$ with staggered fermions is explained. It is shown that the one spin trace operators require additional chiral partner operators in order to keep the continuum chiral behavior. The renormalization of the one spin trace operators is described and compared with the two spin trace formalism.Comment: 47 pages, latex, 4 figures are available on reques

Symmetries of the Kac-Peterson Modular Matrices of Affine Algebras

The characters $\chi_\mu$ of nontwisted affine algebras at fixed level define in a natural way a representation $R$ of the modular group $SL_2(Z)$. The matrices in the image $R(SL_2(Z))$ are called the Kac-Peterson modular matrices, and describe the modular behaviour of the characters. In this paper we consider all levels of $(A_{r_1}\oplus\cdots\oplus A_{r_s})^{(1)}$, and for each of these find all permutations of the highest weights which commute with the corresponding Kac-Peterson matrices. This problem is equivalent to the classification of automorphism invariants of conformal field theories, and its solution, especially considering its simplicity, is a major step toward the classification of all Wess-Zumino-Witten conformal field theories.Comment: 16 pp, plain te