Positive emotion regulation strategies as mediators in depression and generalized anxiety disorder symptoms: A Transdiagnostic framework investigation

Positive emotion regulation (ER) strategies may contribute to the development and maintenance of generalized anxiety disorder (GAD) and depression; nonetheless, the underlying and transdiagnostic mechanisms are still unknown. To examine: 1) the mediating role of positive ER strategies in the relationship between ER deficits and experiential avoidance in GAD and depression symptoms; 2) differences in positive ER strategies among participants with elevated symptoms of GAD, depression, depression comorbid with GAD, and a nonclinical sample. 532 Tehran University students completed questionnaires assessing experiential avoidance, deficits in ER, positive ER strategies (dampening, positive rumination), and GAD and depression symptoms. Differences were assessed in four groups: GAD, depression, depression comorbid with GAD, and nonclinical. Data were analysed with path analysis and MANCOVA. Dampening and positive rumination mediated the relationship between deficits in ER and experiential avoidance in participants with depression and GAD symptoms. As for the between groups comparisons, the comorbid group obtained the highest scores on dampening and the nonclinical group the lowest. By contrast, nonclinical participants had the highest scores on positive rumination, whereas the comorbid group had the lowest. Positive ER strategies might be transdiagnostic variables. Dampening was more associated with psychopathology than positive rumination, suggesting that this strategy could be more clearly considered a transdiagnostic factor. Future studies with clinical samples must support this suggestion

Comparable vitamin D3 metabolism in the endometrium of patients with recurrent spontaneous abortion and fertile controls

Vitamin D exerts important roles during pregnancy, and its deficiency may be associated with several pregnancy complications, including pregnancy loss, yet no data are available for molecules involved in vitamin D metabolism in patients with unexplained recurrent spontaneous abortion. In this study, we investigated possible difference in endometrial expression of vitamin D3 receptor (VDR), 1α-hydroxylase (CYP27B1), and 24-hydroxylase (CYP24A1) in women with recurrent spontaneous abortion (n=8) and healthy controls (n=8). Gene expression of VDR, CYP27B1, and CYP24A1 was determined by real-time PCR, while VDR and CYP27B1 proteins were localized by immunohistochemistry and their abundance was validated by Western blot. We found that both patient and control groups expressed comparable levels of endometrial VDR, CYP27B1, and CYP24A1 transcripts. In line with the gene-expression results, CYP27B1 and different isoforms of VDR protein were present at the same abundance in the endometria of both groups. No significant alteration in VDR and CYP27B1 immunoreactivity pattern was found in the endometrium of patients compared to fertile controls, however. The results of the present study, therefore, do not support the hypothesis of differential expression of key molecules involved in vitamin D3 metabolism in the endometrium of recurrent spontaneous abortion patients and fertile controls. © 2015 Wiley Periodicals, Inc.

From RFLP to DArT: molecular tools for wheat (Triticum spp.) diversity analysis

Wheat (Triticum spp.) is a universally lucrative agricultural crop. An increase in wheat production has been shown through selection by the farmers which can increase the grain profitability. The determination of genetic associations among domestic cultivars is facilitated by molecular markers. Data on genetic polymorphism is valuable for the germplasm association and regarding the developing management strategies. The information would be supportive for potential genome mapping programs and for the relevance of intellectual property rights of wheat breeders. Present review is an effort for providing support information to wheat breeders to develop varieties with varied genetic environment to attain continuity in large-scale wheat production. In this review, we have tried to provide a collective depiction of relevant information about the usage of some commonly used markers in wheat. It may help researchers to find out the frequentness and application of different markers and compare their results. The manuscript may serve as a platform helping the intellectuals for the selection and modification of their marker system in wheat diversity analysis. The heart of this review is the emphasis on the performance of various molecular genetic markers in diversity studies in relation to definite approaches that are in practice since several years allied with the multifaceted wheat molecular breeding and its polyploid nature