Magnetic field detection in the B2Vn star HR 7355

The B2Vn star HR 7355 is found to be a He-rich magnetic star. Spectropolarimetric data were obtained with FORS1 at UT2 on Paranal observatory to measure the disk-averaged longitudinal magnetic field at various phases of the presumed 0.52 d cycle. A variable magnetic field with strengths between B_z = -2200 and +3200G was found, with confidence limits of 100 to 130G. The field topology is that of an oblique dipole, while the star itself is seen about equator-on. In the intensity spectra the HeI-lines show the typical equivalent width variability of He-strong stars, usually attributed to surface abundance spots. The amplitudes of the equivalent width variability of the HeI lines are extraordinarily strong compared to other cases. These results not only put HR 7355 unambiguously among the early-type magnetic stars, but confirm its outstanding nature: With v sin i = 320 km/s the parameter space in which He-strong stars are known to exist has doubled in terms of rotational velocity.Comment: 6 pages, 5 figures, 1 Table. Accepted for publication in MNRAS Letter

Cyclic Variability of the Circumstellar Disc of the Be Star ζ\zeta Tau. II. Testing the 2D Global Disc Oscillation Model

Aims. In this paper we model, in a self-consistent way, polarimetric, photometric, spectrophotometric and interferometric observations of the classical Be star $\zeta$ Tauri. Our primary goal is to conduct a critical quantitative test of the global oscillation scenario. Methods. We have carried out detailed three-dimensional, NLTE radiative transfer calculations using the radiative transfer code HDUST. For the input for the code we have used the most up-to-date research on Be stars to include a physically realistic description for the central star and the circumstellar disc. We adopt a rotationally deformed, gravity darkened central star, surrounded by a disc whose unperturbed state is given by a steady-state viscous decretion disc model. We further assume that disc is in vertical hydrostatic equilibrium. Results. By adopting a viscous decretion disc model for $\zeta$ Tauri and a rigorous solution of the radiative transfer, we have obtained a very good fit of the time-average properties of the disc. This provides strong theoretical evidence that the viscous decretion disc model is the mechanism responsible for disc formation. With the global oscillation model we have successfully fitted spatially resolved VLTI/AMBER observations and the temporal V/R variations of the H$\alpha$ and Br$\gamma$ lines. This result convincingly demonstrates that the oscillation pattern in the disc is a one-armed spiral. Possible model shortcomings, as well as suggestions for future improvements, are also discussed.Comment: 14 pages, 9 figures, accepted to A&

Optical variabilities in Be/X-ray binary system:GRO J2058+42

We present an analysis of long-term optical monitoring observations and optical spectroscopic observations of the counterpart to CXOU J205847.5+414637 (high mass X-ray binary system). We search for a variability in the light curve of Be star. We used differential magnitudes in the time series analysis. The variability search in the optical light curve was made by using different algorithms. The reduction and analysis of spectra were done by using MIDAS and its suitable packages. We have performed a frequency search which gave us the value 2.404 1/day. This value is attributed to the non-radial pulsation of Be star. H alpha emission line profiles always show double-peaked emissions with a mean equivalent width of 2.31 \pm 0.19 \AA ~and a peak separation of 516 \pm 45 km/s. This suggests that Be star disk is still present. CXOU J205847.5+414637 is in X-ray quiescent state.Comment: 8 pages, 9 figures. To appear at Astronomy and Astrophysic

ZC4H2, an XLID gene, is required for the generation of a specific subset of CNS interneurons

Miles-Carpenter syndrome (MCS) was described in 1991 as an XLID syndrome with fingertip arches and contractures and mapped to proximal Xq. Patients had microcephaly, short stature, mild spasticity, thoracic scoliosis, hyperextendable MCP joints, rocker-bottom feet, hyperextended elbows and knees. A mutation, p.L66H, in ZC4H2, was identified in a XLID resequencing project. Additional screening of linked families and next generation sequencing of XLID families identified three ZC4H2 mutations: p.R18K, p.R213W and p.V75in15aa. The families shared some relevant clinical features. In silico modeling of the mutant proteins indicated all alterations would destabilize the protein. Knockout mutations in zc4h2 were created in zebrafish and homozygous mutant larvae exhibited abnormal swimming, increased twitching, defective eye movement and pectoral fin contractures. Because several of the behavioral defects were consistent with hyperactivity, we examined the underlying neuronal defects and found that sensory neurons and motoneurons appeared normal. However, we observed a striking reduction in GABAergic interneurons. Analysis of cell-type-specificmarkers showed a specific loss of V2 interneurons in the brain and spinal cord, likely arising from mis-specification of neural progenitors. Injected human wt ZC4H2 rescued the mutant phenotype. Mutant zebrafish injectedwith human p.L66H or p.R213W mRNA failed to be rescued, while the p.R18K mRNA was able to rescue the interneuron defect. Our findings clearly support ZC4H2 as a novel XLID gene with a required function in interneuron development. Loss of function of ZC4H2 thus likely results in altered connectivity ofmany brain and spinal circuits

Weak Glycolipid Binding of a Microdomain-Tracer Peptide Correlates with Aggregation and Slow Diffusion on Cell Membranes

10.1371/journal.pone.0051222PLoS ONE712

Low potency toxins reveal dense interaction networks in metabolism

Background The chemicals of metabolism are constructed of a small set of atoms and bonds. This may be because chemical structures outside the chemical space in which life operates are incompatible with biochemistry, or because mechanisms to make or utilize such excluded structures has not evolved. In this paper I address the extent to which biochemistry is restricted to a small fraction of the chemical space of possible chemicals, a restricted subset that I call Biochemical Space. I explore evidence that this restriction is at least in part due to selection again specific structures, and suggest a mechanism by which this occurs. Results Chemicals that contain structures that our outside Biochemical Space (UnBiological groups) are more likely to be toxic to a wide range of organisms, even though they have no specifically toxic groups and no obvious mechanism of toxicity. This correlation of UnBiological with toxicity is stronger for low potency (millimolar) toxins. I relate this to the observation that most chemicals interact with many biological structures at low millimolar toxicity. I hypothesise that life has to select its components not only to have a specific set of functions but also to avoid interactions with all the other components of life that might degrade their function. Conclusions The chemistry of life has to form a dense, self-consistent network of chemical structures, and cannot easily be arbitrarily extended. The toxicity of arbitrary chemicals is a reflection of the disruption to that network occasioned by trying to insert a chemical into it without also selecting all the other components to tolerate that chemical. This suggests new ways to test for the toxicity of chemicals, and that engineering organisms to make high concentrations of materials such as chemical precursors or fuels may require more substantial engineering than just of the synthetic pathways involved

Environmental factors modulating the stability and enzymatic activity of the Petrotoga mobilis Esterase (PmEst)

Enzymes isolated from thermophilic organisms found in oil reservoirs can find applications in many fields, including the oleochemical, pharmaceutical, bioenergy, and food/dairy industries. In this study, in silico identification and recombinant production of an esterase from the extremophile bacteria Petrotoga mobilis (designated PmEst) were performed. Then biochemical, bioinformatics and structural characterizations were undertaken using a combination of synchrotron radiation circular dichroism (SRCD) and fluorescence spectroscopies to correlate PmEst stability and hydrolytic activity on different substrates. The enzyme presented a high Michaelis-Menten constant (KM 0.16 mM) and optimum activity at ~55°C for p-nitrophenyl butyrate. The secondary structure of PmEst was preserved at acid pH, but not under alkaline conditions. PmEst was unfolded at high concentrations of urea or guanidine through apparently different mechanisms. The esterase activity of PmEst was preserved in the presence of ethanol or propanol and its melting temperature increased ~8°C in the presence of these organic solvents. PmEst is a mesophilic esterase with substrate preference towards short-to medium-length acyl chains. The SRCD data of PmEst is in agreement with the prediction of an α/β protein, which leads us to assume that it displays a typical fold of esterases from this family. The increased enzyme stability in organic solvents may enable novel applications for its use in synthetic biology. Taken together, our results demonstrate features of the PmEst enzyme that indicate it may be suitable for applications in industrial processes, particularly, when the use of polar organic solvents is required