Bioluminescence intensity modeling and sampling strategy optimization

Author Posting. © American Meteorological Society 2005. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Atmospheric and Oceanic Technology 22 (2005): 1267–1281, doi:10.1175/JTECH1760.1.The focus of this paper is on the development of methodology for short-term (1–3 days) oceanic bioluminescence (BL) predictions and the optimization of spatial and temporal bioluminescence sampling strategies. The approach is based on predictions of bioluminescence with an advection–diffusion–reaction (tracer) model with velocities and diffusivities from a circulation model. In previous research, it was shown that short-term changes in some of the salient features in coastal bioluminescence can be explained and predicted by using this approach. At the same time, it was demonstrated that optimization of bioluminescence sampling prior to the forecast is critical for successful short-term BL predictions with the tracer model. In the present paper, the adjoint to the tracer model is used to study the sensitivity of the modeled bioluminescence distributions to the sampling strategies for BL. The locations and times of bioluminescence sampling prior to the forecast are determined by using the adjoint-based sensitivity maps. The approach is tested with bioluminescence observations collected during August 2000 and 2003 in the Monterey Bay, California, area. During August 2000, BL surveys were collected during a strong wind relaxation event, while in August 2003, BL surveys were conducted during an extended (longer than a week) upwelling-favorable event. The numerical bioluminescence predictability experiments demonstrated a close agreement between observed and model-predicted short-term spatial and temporal changes of the coastal bioluminescence.This work has been supported by the Ocean Optics and Biology and Physical Oceanography Programs of the Office of Naval Research. Shulman’s support is through the NRL “Use of a Circulation Model to Enhance Predictability of Bioluminescence in the Coastal Ocean” project sponsored by the Office of Naval Research

Vascular Endothelial Growth Factor mRNA Isoforms 120 and 164 are Differentially Regulated Prior to Ovulation

Vascular Endothelial Growth Factor (VEGF) is produced by cells surrounding the egg in the follicle prior to ovulation. If VEGF is inhibited, ovulation does not occur. The VEGF gene can be spliced to produce different protein isoforms which have specific functions. Our objective was to determine if VEGF 120 and 164 mRNA isoforms are differentially regulated in the preovulatory follicle. From our studies, VEGF isoforms are differentially regulated during both CL regression and after a simulated LH surge. Differences observed in VEGF isoform regulation may allow for manipulation of ovulation in the beef cow

Vascular Endothelial Growth Factor mRNA Isoforms 120 and 164 are Differentially Regulated Prior to Ovulation

Vascular Endothelial Growth Factor (VEGF) is produced by cells surrounding the egg in the follicle prior to ovulation. If VEGF is inhibited, ovulation does not occur. The VEGF gene can be spliced to produce different protein isoforms which have specific functions. Our objective was to determine if VEGF 120 and 164 mRNA isoforms are differentially regulated in the preovulatory follicle. From our studies, VEGF isoforms are differentially regulated during both CL regression and after a simulated LH surge. Differences observed in VEGF isoform regulation may allow for manipulation of ovulation in the beef cow

Platform effects on optical variability and prediction of underwater visibility

We present hydrographic and optical data collected concurrently from two different platforms, the R/P FLoating Instrument Platform and the R/V Kilo Moana, located about 2km apart in the Santa Barbara Channel in California. We show that optical variability between the two platforms was due primarily to platform effects, specifically the breakdown of stratification from mixing by the hull of R/P FLIP. Modeled underwater radiance distribution differed by as much as 50% between the two platforms during stratified conditions. We determine that the observed optical variability resulted in up to 57% differences in predicted horizontal visibility of a black target

Cancer in general responders participating in world trade center health programs, 2003-2013

© The Author(s) 2020. Published by Oxford University Press. Background: Following the September 11, 2001, attacks on the World Trade Center (WTC), thousands of workers were exposed to an array of toxins known to cause adverse health effects, including cancer. This study evaluates cancer incidence in the WTC Health Program General Responder Cohort occurring within 12 years post exposure. Methods: The study population consisted of 28729 members of the General Responder Cohort enrolled from cohort inception, July 2002 to December 31, 2013. Standardized incidence ratios (SIRs) were calculated with cancer case inclusion and follow-up starting post September 11, 2001 (unrestricted) and, alternatively, to account for selection bias, with case inclusion and follow-up starting 6 months after enrollment in the WTC Health Program (restricted). Case ascertainment was based on linkage with six state cancer registries. Under the restricted criterion, hazard ratios were estimated using multivariable Cox proportional hazards models for all cancer sites combined and for prostate cancer. Results: Restricted analyses identified 1072 cancers in 999 responders, with elevations in cancer incidence for all cancer sites combined (SIR = 1.09, 95% confidence interval [CI] = 1.02 to 1.16), prostate cancer (SIR = 1.25, 95% CI = 1.11 to 1.40), thyroid cancer (SIR = 2.19, 95% CI = 1.71 to 2.75), and leukemia (SIR = 1.41, 95% CI = 1.01 to 1.92). Cancer incidence was not associated with any WTC exposure index (composite or individual) for all cancer sites combined or for prostate cancer. Conclusion: Our analyses show statistically significant elevations in cancer incidence for all cancer sites combined and for prostate and thyroid cancers and leukemia. Multivariable analyses show no association with magnitude or type of exposure

An evaluation of peptone products and fish meal on nursery pig performance

A total of 360 nursery pigs (PIC C327 × 1050, initially 11.8 lb and 21 d of age) were used in a 35-d study to evaluate the effects of select menhaden fish meal (SMFM), PEP2+ (also known as Ferm O Tide), Peptone 50, and PEP-NS on nursery pig performance. PEP2+, Peptone 50, and PEP-NS are all porcine intestinal mucosa products, but differ based on the carriers with which they are co-dried. PEP2+ is co-dried with enzymatically processed vegetable proteins. Peptone 50 is co-dried with a vegetable protein, while PEP-NS uses by-products from corn wet-milling. Phase 1 diets were fed in pellet form from d 0 to 8. Phase 2 diets were fed in meal form from d 8 to 21. A common corn-soybean meal diet was fed from d 21 to 35. There were 6 dietary treatments: (1) a negative control diet containing 2.5% spray-dried animal plasma (SDAP) in Phase 1 followed by no specialty protein sources in Phase 2; (2) a diet containing 5% SDAP in Phase 1 and 3% SMFM in Phase 2; (3) a blend of 5% SDAP and 3% SMFM during Phase 1 and 6% SMFM during Phase 2; (4) a blend of 5% SDAP and 3% PEP2+ during Phase 1 and 6% PEP2 during Phase 2; (5) a blend of 5% SDAP and 3% PEP 50 during Phase 1 and 6% PEP50 during Phase 2, and (6) a blend of 5% SDAP and 3% PEP-NS during Phase 1 and 6% PEP-NS during Phase 2. During Phase 1, there were no differences in F/G among pigs fed any of the dietary treatments. During Phase 2 (d 8 to 21), pigs fed 6% PEP2+ had greater (P < 0.05) ADG compared to those fed the negative control diet, 3% or 6% fish meal, with pigs fed PEP50 and PEP NS intermediate. Furthermore, pigs fed 6% PEP2+ had the greatest improvement (P < 0.02) in F/G compared to pigs fed all other experimental diets. Overall, pigs fed diets containing PEP2+ had increased (P < 0.03) ADG and ADFI compared to pigs fed the negative control diet. Pigs fed 3% PEP2+ during Phase 1 and 6% PEP2+ during Phase 2 had greater (P < 0.05) ADFI compared to those fed 3% SMFM during Phase 1 and 6% SMFM during Phase 2. In conclusion, PEP2+, Peptone 50, and PEP-NS can be used as specialty protein sources to replace select menhaden fish meal in Phase 2 nursery pig diets. In addition pigs fed PEP2+ had greater ADG than those fed fish meal

Reproductive Aging Influences Ovarian Function in Beef Cows

Anti-Müllerian Hormone (AMH) has been associated with follicle number and age of the ovary. Therefore, our hypothesiswas that AMH was a biomarker for both follicle number and ovarian function in the beef cow. Ovaries were collected by flank laparotomy. The number of follicles increased as cows aged from 1.5 to 6 years and began to decrease thereafter; however, the size of the ovary continued to increase with advanced age. Expression of the AMH gene increased with increasing follicle number in 2-year-old beef cows. These results suggest that heifers with larger ovaries will have greater numbers of follicles and greater productivity, allowing them to stay in the production herd longer. AMH could be used to identify heifers of high reproductive potential at a very young age