1,971 research outputs found
Cellular location and activity of Escherichia coli RecG proteins shed light on the function of its structurally unresolved C-terminus
RecG is a DNA translocase encoded by most species of bacteria. The Escherichia coli protein targets branched DNA substrates and drives the unwinding and rewinding of DNA strands. Its ability to remodel replication forks and to genetically interact with PriA protein have led to the idea that it plays an important role in securing faithful genome duplication. Here we report that RecG co-localises with sites of DNA replication and identify conserved arginine and tryptophan residues near its C-terminus that are needed for this localisation. We establish that the extreme C-terminus, which is not resolved in the crystal structure, is vital for DNA unwinding but not for DNA binding. Substituting an alanine for a highly conserved tyrosine near the very end results in a substantial reduction in the ability to unwind replication fork and Holliday junction structures but has no effect on substrate affinity. Deleting or substituting the terminal alanine causes an even greater reduction in unwinding activity, which is somewhat surprising as this residue is not uniformly present in closely related RecG proteins. More significantly, the extreme C-terminal mutations have little effect on localisation. Mutations that do prevent localisation result in only a slight reduction in the capacity for DNA repair. © 2014 The Author(s)
Inverse Magnetoresistance of Molecular Junctions
We present calculations of spin-dependent electron transport through single
organic molecules bridging pairs of iron nanocontacts. We predict the
magnetoresistance of these systems to switch from positive to negative with
increasing applied bias for both conducting and insulating molecules. This
novel inverse magnetoresistance phenomenon is robust, does not depend on the
presence of impurities, and is unique to molecular and atomic nanoscale
magnetic junctions. Its physical origin is identified and its relevance to
experiment and to potential technological applications is discussed.Comment: 5 pages, 3 figures; published version Phys. Rev.
Theoretical Study of Spin-dependent Electron Transport in Atomic Fe Nanocontacts
We present theoretical predictions of spintronic transport phenomena that
should be observable in ferromagnetic Fe nanocontacts bridged by chains of Fe
atoms. We develop appropriate model Hamiltonians based on semi-empirical
considerations and the known electronic structure of bulk Fe derived from ab
initio density functional calculations. Our model is shown to provide a
satisfactory description of the surface properties of Fe nano-clusters as well
as bulk properties. Lippmann-Schwinger and Green's function techniques are used
together with Landauer theory to predict the current, magneto-resistance, and
spin polarization of the current in Fe nanocontacts bridged by atomic chains
under applied bias. Unusual device characteristics are predicted including
negative magneto-resistance and spin polarization of the current, as well as
spin polarization of the current for anti-parallel magnetization of the Fe
nanocontacts under moderate applied bias. We explore the effects that
stretching the atomic chain has on the magneto-resistance and spin polarization
and predict a cross-over regime in which the spin polarization of the current
for parallel magnetization of the contacts switches from negative to positive.
We find resonant transmission due to dangling bond formation on tip atoms as
the chain is stretched through its breaking point to play an important role in
spin-dependent transport in this regime. The physical mechanisms underlying the
predicted phenomena are discussed.Comment: 13 pages, 6 figures, Accepted for publication in Physical Review
Ras/Raf-1/MAPK pathway mediates response to tamoxifen but not chemotherapy in breast cancer patients
<b>Purpose</b>: The expression and activation of the Ras/Raf-1/mitogen-activated protein kinase (MAPK) pathway plays an important role in the development and progression of cancer, and may influence response to treatments such as tamoxifen and chemotherapy. In this study we investigated whether the expression and activation of the key components of this pathway influenced clinical outcome, to test the hypothesis that activation of the MAPK pathway drives resistance to tamoxifen and chemotherapy in women with breast cancer.
<b>Experimental Design</b>: Breast tumors from patients at the Glasgow Royal Infirmary and others treated within the BR9601 trial were analyzed for expression of the three Ras isoforms, total Raf-1, active and inactive forms of Raf-1 [pRaf(ser338) and pRaf(ser259), respectively], MAPK, and phospho-MAPK using an immunohistochemical approach. Analyses were done with respect to disease free-survival and overall survival.
<b>Results</b>: Expression and activation of the Ras pathway was associated with loss of benefit from treatment with tamoxifen but not chemotherapy. Overexpression of pRaf(ser338) was associated with shortened disease-free and overall survival time in univariate analyses. Multivariate analysis suggested pRaf(ser338) was independent of known prognostic markers in predicting outcome following tamoxifen treatment (<i>P</i>=0.03).
<b>Conclusion</b>: This study suggests that activation of the Ras pathway predicts for poor outcome on tamoxifen but not chemotherapy, and identifies pRaf(ser338) as a potential marker of resistance to estrogen receptor–targeted therapy. In addition, it suggests that expression of pRaf(ser338) could identify patients for whom tamoxifen alone is insufficient adjuvant systemic therapy, but for whom the addition of chemotherapy may be of benefit
Extremely narrow spectrum of GRB110920A: further evidence for localised, subphotospheric dissipation
Much evidence points towards that the photosphere in the relativistic outflow
in GRBs plays an important role in shaping the observed MeV spectrum. However,
it is unclear whether the spectrum is fully produced by the photosphere or
whether a substantial part of the spectrum is added by processes far above the
photosphere. Here we make a detailed study of the ray emission from
single pulse GRB110920A which has a spectrum that becomes extremely narrow
towards the end of the burst. We show that the emission can be interpreted as
Comptonisation of thermal photons by cold electrons in an unmagnetised outflow
at an optical depth of . The electrons receive their energy by a
local dissipation occurring close to the saturation radius. The main spectral
component of GRB110920A and its evolution is thus, in this interpretation,
fully explained by the emission from the photosphere including localised
dissipation at high optical depths.Comment: 14 pages, 11 figures, accepted to MNRA
Comparison of fission and quasi-fission modes
Quantum shell effects are known to affect the formation of fragments in
nuclear fission. Shell effects also affect quasi-fission reactions occurring in
heavy-ion collisions. Systematic time-dependent Hartree-Fock simulations of
50Ca+176Yb collisions show that the mass equilibration between the fragments in
quasi-fission is stopped when they reach similar properties to those in the
asymmetric fission mode of the 226Th compound nucleus. Similar shell effects
are then expected to determine the final repartition of nucleons between the
nascent fragments in both mechanisms. Future experimental studies that could
test these observations are discussed.Comment: 8 pages, 4 figures, 1 tabl
Structural, optical and magnetic properties of Zn1−xMnxO micro-rod arrays synthesized by spray pyrolysis method
Undoped and Mn-doped ZnO micro-rod arrays were fabricated by the spray pyrolysis method on glass substrates. X-ray diffraction and scanning electron microscopy showed that these micro-rod arrays had a polycrystalline wurtzite structure and high c-axis preferred orientation. Photoluminescence studies at 10 K show that the increase of manganese content leads to a relative decrease in deep level band intensity with respect to undoped ZnO. Magnetic measurements indicated that undoped ZnO was diamagnetic in nature whereas Mn-doped ZnO samples exhibited ferromagnetic behavior at room temperature, which is possibly related to the substitution of Mn ions (Mn2+) for Zn ions in the ZnO lattice
Evidence for a Photospheric Component in the Prompt Emission of the Short GRB120323A and its Effects on the GRB Hardness-Luminosity Relation
The short GRB 120323A had the highest flux ever detected with the Fermi/GBM.
Here we study its remarkable spectral properties and their evolution using two
spectral models: (i) a single emission component scenario, where the spectrum
is modeled by the empirical Band function, and (ii) a two component scenario,
where thermal (Planck-like) emission is observed simultaneously with a
non-thermal component (a Band function). We find that the latter model fits the
integrated burst spectrum significantly better than the former, and that their
respective spectral parameters are dramatically different: when fit with a Band
function only, the Epeak of the event is unusually soft for a short GRB, while
adding a thermal component leads to more typical short GRB values. Our
time-resolved spectral analysis produces similar results. We argue here that
the two-component model is the preferred interpretation for GRB 120323A, based
on: (i) the values and evolution of the Band function parameters of the two
component scenario, which are more typical for a short GRB, and (ii) the
appearance in the data of a significant hardness-intensity correlation,
commonly found in GRBs, when we employee two-component model fits; the
correlation is non-existent in the Band-only fits. GRB 110721A, a long burst
with an intense photospheric emission, exhibits the exact same behavior. We
conclude that GRB 120323A has a strong photospheric emission contribution,
first time observed in a short GRB. Magnetic dissipation models are difficult
to reconcile with these results, which instead favor photospheric thermal
emission and fast cooling synchrotron radiation from internal shocks. Finally,
we derive a possibly universal hardness-luminosity relation in the source frame
using a larger set of GRBs L,i=(1.59+/-0.84).10^50 (Epeak,i)^(1.33+/-0.07)
erg/s), which could be used as a possible redshift estimator for cosmology.Comment: 27 pages, 13 figures, Accepted by ApJ (April, 7th 2013
Discovery of a New Nearby Star
We report the discovery of a nearby star with a very large proper motion of
5.06 +/- 0.03 arcsec/yr. The star is called SO025300.5+165258 and referred to
herein as HPMS (high proper motion star). The discovery came as a result of a
search of the SkyMorph database, a sensitive and persistent survey that is well
suited for finding stars with high proper motions. There are currently only 7
known stars with proper motions > 5 arcsec/yr. We have determined a preliminary
value for the parallax of 0.43 +/- 0.13 arcsec. If this value holds our new
star ranks behind only the Alpha Centauri system (including Proxima Centauri)
and Barnard's star in the list of our nearest stellar neighbors. The spectrum
and measured tangential velocity indicate that HPMS is a main-sequence star
with spectral type M6.5. However, if our distance measurement is correct, the
HPMS is underluminous by 1.2 +/- 0.7 mag.Comment: 5 pages, 3 figures. Submitted to ApJ Letter
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