Translation initiation factor eIF3 promotes programmed stop codon readthrough.

Programmed stop codon readthrough is a post-transcription regulatory mechanism specifically increasing proteome diversity by creating a pool of C-terminally extended proteins. During this process, the stop codon is decoded as a sense codon by a near-cognate tRNA, which programs the ribosome to continue elongation. The efficiency of competition for the stop codon between release factors (eRFs) and near-cognate tRNAs is largely dependent on its nucleotide context; however, the molecular mechanism underlying this process is unknown. Here, we show that it is the translation initiation (not termination) factor, namely eIF3, which critically promotes programmed readthrough on all three stop codons. In order to do so, eIF3 must associate with pre-termination complexes where it interferes with the eRF1 decoding of the third/wobble position of the stop codon set in the unfavorable termination context, thus allowing incorporation of near-cognate tRNAs with a mismatch at the same position. We clearly demonstrate that efficient readthrough is enabled by near-cognate tRNAs with a mismatch only at the third/wobble position. Importantly, the eIF3 role in programmed readthrough is conserved between yeast and humans

Welzijn (opfok) vleeskuikenouderdieren

Praktijkcentrum 'Het Spelderholt' heeft in samenwerking met ID-Lelystad onderzocht of tweemaal in plaats van eenmaal per dag voeren in de opfokperiode het welzijn van vleeskuikenouderdieren verbetert. Hiermee werd niet het verwachte effect op het gedrag verkregen

Gaussian noise and time-reversal symmetry in non-equilibrium Langevin models

We show that in driven systems the Gaussian nature of the fluctuating force and time-reversibility are equivalent properties. This result together with the potential condition of the external force drastically restricts the form of the probability distribution function, which can be shown to satisfy time-independent relations. We have corroborated this feature by explicitly analyzing a model for the stretching of a polymer and a model for a suspension of non-interacting Brownian particles in steady flow.Comment: 6 pages, submitted to PR

Investor protection through model case procedures – implementing collective goals and individual rights under the 2012 Amendment of the German Capital Markets Model Case Act (KapMuG)

The German Capital Markets Model Case Act (KapMuG) and its amendment of 2012 highlight some fundamentals of collective redress in civil law countries at the example of model case procedures in the field of investor protection. That is why a survey of the ongoing activities of the European Union in the area of collective redress and of its repercussions on the member state level forms a suitable basis for the following analysis of the 2012 amendment of the KapMuG. It clearly brings into focus a shift from sector-specific regulation with an emphasis on the cross-border aspect of protecting consumers towards a “coherent approach” strengthening the enforcement of EU law. As a result, regulatory policy and collective redress are two sides of the same coin today. With respect to the KapMuG such a development brings about some tension between its aim to aggregate small individual claims as efficiently as possible and the dominant role of individual procedural rights in German civil procedure. This conflict can be illustrated by some specific rules of the KapMuG: its scope of application, the three-tier procedure of a model case procedure, the newly introduced notification of claims and the new opt-out settlement under the amended §§ 17-19

Eu-Eu exchange interaction and Eu distribution in Pb_(1-x)Eu_(x)Te from magnetization steps

The magnetization of Pb_{1-x}Eu_{x}Te samples with x = 1.9, 2.6 and 6.0% was measured at 20 mK in fields up to 50 kOe, and at 0.6 K in fields up to 180 kOe. The 20 mK data show the magnetization steps (MSTs) arising from pairs and from triplets. The pair MSTs are used to obtain the dominant Eu-Eu antiferromagnetic exchange constant, J/k_{B} = -0.264 \pm 0.018 K. The exchange constant for triplets is the same. Comparison of the magnetization curves with theoretical simulations indicates that the Eu ions are not randomly distributed over all the cation sites. The deviation from a random distribution is much smaller if J is assumed to be the nearest-neighbor exchange constant J_{1} rather than the next-nearest-neighbor exchange constant J_{2}. On this basis, J is tentatively identified as J_{1}. To obtain agreement with the data, it must be assumed that the Eu ions tend to bunch together. Comparision with microprobe data indicates that the length scale for these concentration variations is smaller than a few micrometer. The theoretical simulations in the present work improve on those performed earlier by including clusters larger than three spins.Comment: 9 pages, 6 figs, Revtex, accepted for publication in Phys. Rev.

The Chandrasekhar limit for quark stars

The Chandrasekhar limit for quark stars is evaluated from simple energy balance relations, as proposed by Landau for white dwarfs or neutron stars. It has been found that the limit for quark stars depends on, in addition to the fundamental constants, the Bag constant.Comment: LateX fil

Relative momentum for identical particles

Possible definitions for the relative momentum of identical particles are considered

Bounded solutions of neutral fermions with a screened Coulomb potential

The intrinsically relativistic problem of a fermion subject to a pseudoscalar screened Coulomb plus a uniform background potential in two-dimensional space-time is mapped into a Sturm-Liouville. This mapping gives rise to an effective Morse-like potential and exact bounded solutions are found. It is shown that the uniform background potential determinates the number of bound-state solutions. The behaviour of the eigenenergies as well as of the upper and lower components of the Dirac spinor corresponding to bounded solutions is discussed in detail and some unusual results are revealed. An apparent paradox concerning the uncertainty principle is solved by recurring to the concepts of effective mass and effective Compton wavelength.Comment: 15 pages, 7 figure

Evaluation of radiography as a screening method for detection and characterisation of congenital vertebral malformations in dogs

Congenital vertebral malformations (CVM) are common in brachycephalic ‘screw-tailed’ dogs; they can be associated with neurological deficits and a genetic predisposition has been suggested. The purpose of this study was to evaluate radiography as a screening method for congenital thoracic vertebral malformations in brachycephalic ‘screw-tailed’ dogs by comparing it with CT. Forty-nine dogs that had both radiographic and CT evaluations of the thoracic vertebral column were included. Three observers retrospectively reviewed the images independently to detect CVMs. When identified, they were classified according to a previously published radiographic classification scheme. A CT consensus was then reached. All observers identified significantly more affected vertebrae when evaluating orthogonal radiographic views compared with lateral views alone; and more affected vertebrae with the CT consensus compared with orthogonal radiographic views. Given the high number of CVMs per dog, the number of dogs classified as being CVM free was not significantly different between CT and radiography. Significantly more midline closure defects were also identified with CT compared with radiography. Malformations classified as symmetrical or ventral hypoplasias on radiography were frequently classified as ventral and medial aplasias on CT images. Our results support that CT is better than radiography for the classification of CVMs and this will be important when further evidence of which are the most clinically relevant CVMs is identified. These findings are of particular importance for designing screening schemes of CVMs that could help selective breeding programmes based on phenotype and future studies