Pupil Dilation Differences When Playing Valorant Under Practice and Competition Conditions: A Case Study

Esports have been growing faster than any other sport in history. Esports are video games that are played in official competitions and usually fall into a major genre, such as fighting games, real-time strategy, multiplayer online battle arena games, or first-person shooters (FPS). Valorant is an FPS. Advances in technology have now made a new class of information, namely biofeedback, readily available. An example of biofeedback is pupil size, which is an indirect measure of the amount of material under active processing and sympathetic/parasympathetic activity. Eye behavior is considered crucial in FPS games. Relevant research is scarce in this specific esport genre. Skills learned during practice should transfer to real-game environments. PURPOSE: To investigate differences in pupil size between practice and competition sessions of Valorant. METHODS: A 21-year-old collegiate esports player, ranked Diamond 1 (top 12%), recorded a practice session and, then, a game of Valorant on the same day. Each session lasted about 65 minutes. Valorant is a 5v5 character-based tactical FPS game. Data was collected via Curia software using LabStreamingLayer to stream data from a Tobii 5L eye tracker into LSL native XDF format, retaining timestamping and synchronization information. Due to having one participant, significance was investigated graphically. RESULTS: On average, the pupil size during practice was 5.1mm and during game 5.3mm. When comparing the mean pupil diameter of both eyes between sessions using violin and scatterplot graphs, it was observed that the distribution of the pupil sizes in the game was shifted higher than during the practice session. CONCLUSION: Our findings indicate higher cognitive load and sympathetic innervation/parasympathetic withdrawal during competition versus practice conditions. Implications for evidence-based practice include replication of load during practice to accomplish game-like conditions, and therefore, facilitate optimal learning and development of expertise. The significance of these findings increases as this form of augmented feedback can be available to the athlete and the coach in real time. Future research should consider examining fixation stability/location, the relationship of pupil dilation with HRV and using longitudinal designs and larger samples

Outcomes in hepatitis C virus–infected recipients of living donor vs. deceased donor liver transplantation

In this retrospective study of hepatitis C virus (HCV)–infected transplant recipients in the 9-center Adult to Adult Living Donor Liver Transplantation Cohort Study, graft and patient survival and the development of advanced fibrosis were compared among 181 living donor liver transplant (LDLT) recipients and 94 deceased donor liver transplant (DDLT) recipients. Overall 3-year graft and patient survival were 68% and 74% in LDLT, and 80% and 82% in DDLT, respectively. Graft survival, but not patient survival, was significantly lower for LDLT compared to DDLT ( P = 0.04 and P = 0.20, respectively). Further analyses demonstrated lower graft and patient survival among the first 20 LDLT cases at each center (LDLT 20; P = 0.002 and P = 0.002, respectively) and DDLT recipients ( P 20 and DDLT were not significantly different ( P = 0.66 and P = 0.74, respectively). Overall, 3-year graft survival for DDLT, LDLT >20, and LDLT 20 were not significantly different. Important predictors of graft loss in HCV-infected patients were limited LDLT experience, pretransplant HCC, and higher MELD at transplantation. Liver Transpl 13:122–129, 2007. © 2006 AASLD.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/55915/1/20995_ftp.pd

Population screening for liver fibrosis: towards early diagnosis and intervention for chronic liver diseases

Cirrhosis, highly prevalent worldwide, develops after years of hepatic inflammation triggering progressive fibrosis. Currently, the main etiologies of cirrhosis are non-alcoholic fatty liver disease (NAFLD) and alcohol-related liver disease (ALD), although chronic hepatitis B and C infections are still major etiological factors in some areas of the world. Recent studies have shown that liver fibrosis can be assessed with relatively high accuracy non-invasively by serological tests, transient elastography, and radiological methods. These modalities may be utilized for screening for liver fibrosis in at-risk populations. Thus far, a limited number of population-based studies using non-invasive tests in different areas of the world indicate that a significant percentage of subjects without known liver disease (around 5% in general populations and a higher rate -18 to 27%- in populations with risk factors for liver disease) have significant undetected liver fibrosis or established cirrhosis. Larger international studies are required to show the harms and benefits before concluding that screening for liver fibrosis should be applied to populations at risk for chronic liver diseases. Screening for liver fibrosis has the potential for changing the current approach from diagnosing chronic liver diseases late when patients have already developed complications of cirrhosis to diagnosing liver fibrosis in asymptomatic subjects providing the opportunity of preventing disease progression

Biomarkers of disease differentiation: HCV recurrence versus acute cellular rejection

The wound-healing process induced by chronic hepatitis C virus (HCV) infection triggers liver damage characterized by fibrosis development and finally cirrhosis. Liver Transplantation (LT) is the optimal surgical treatment for HCV-cirrhotic patients at end-stage liver disease. However, acute cellular rejection (ACR) and HCV recurrence disease represent two devastating complications post-LT. The accurate differential diagnosis between both conditions is critical for treatment choice, and similar histological features represent a challenge for pathologists. Moreover, the HCV recurrence disease severity is highly variable post-LT. HCV recurrence disease progression is characterized by an accelerated fibrogenesis process, and almost 30% of those patients develop cirrhosis at 5-years of follow-up. Whole-genome gene expression (WGE) analyses through well-defined oligonucleotide microarray platforms represent a powerful tool for the molecular characterization of biological process. In the present manuscript, the utility of microarray technology is applied for the ACR and HCV-recurrence biological characterization in post-LT liver biopsy samples. Moreover, WGE analysis was performed to identify predictive biomarkers of HCV recurrence severity in formalin-fixed paraffin-embedded liver biopsies prospectively collected

Focal Distribution of Hepatitis C Virus RNA in Infected Livers

Background: Hepatitis C virus (HCV) is a plus-strand RNA virus that replicates by amplification of genomic RNA from minus strands leading to accumulation of almost one thousand copies per cell under in vitro cell culture conditions. In contrast, HCV RNA copy numbers in livers of infected patients appear to be much lower, estimated at a few copies per cell. Methodology/Principal Findings: To gain insights into mechanisms that control HCV replication in vivo, we analyzed HCV RNA levels as well as expression of interferon beta (IFNb) and several interferon stimulated genes (ISGs) from whole liver sections and micro-dissected subpopulations of hepatocytes in biopsy samples from 21 HCV-infected patients. The results showed that intrahepatic HCV RNA levels range form less than one copy per hepatocyte to a maximum of about eight. A correlation existed between viral RNA levels and IFNb expression, but not between viral RNA and ISG levels. Also, IFNb expression did not correlate with ISGs levels. Replication of HCV RNA occurred in focal areas in the liver in the presence of a general induction of ISGs. Conclusion/Significance: The low average levels of HCV RNA in biopsy samples can be explained by focal distribution of infected hepatocytes. HCV replication directly induces IFNb, which then activates ISGs. The apparent lack of a correlation between levels of IFNb and ISG expression indicates that control of the innate immune response during HCV infection

LiverScreen project: study protocol for screening for liver fibrosis in the general population in European countries

Background: The development of liver cirrhosis is usually an asymptomatic process until late stages when complications occur. The potential reversibility of the disease is dependent on early diagnosis of liver fibrosis and timely targeted treatment. Recently, the use of non-invasive tools has been suggested for screening of liver fibrosis, especially in subjects with risk factors for chronic liver disease. Nevertheless, large population-based studies with cost-effectiveness analyses are still lacking to support the widespread use of such tools. The aim of this study is to investigate whether non-invasive liver stiffness measurement in the general population is useful to identify subjects with asymptomatic, advanced chronic liver disease. Methods: This study aims to include 30,000 subjects from eight European countries. Subjects from the general population aged ≥ 40 years without known liver disease will be invited to participate in the study either through phone calls/letters or through their primary care center. In the first study visit, subjects will undergo bloodwork as well as hepatic fat quantification and liver stiffness measurement (LSM) by vibration-controlled transient elastography. If LSM is ≥ 8 kPa and/or if ALT levels are ≥1.5 x upper limit of normal, subjects will be referred to hospital for further evaluation and consideration of liver biopsy. The primary outcome is the percentage of subjects with LSM ≥ 8kPa. In addition, a health economic evaluation will be performed to assess the cost-effectiveness and budget impact of such an intervention. The project is funded by the European Commission H2020 program. Discussion: This study comes at an especially important time, as the burden of chronic liver diseases is expected to increase in the coming years. There is consequently an urgent need to change our current approach, from diagnosing the disease late when the impact of interventions may be limited to diagnosing the disease earlier, when the patient is asymptomatic and free of complications, and the disease potentially reversible. Ultimately, the LiverScreen study will serve as a basis from which diagnostic pathways can be developed and adapted to the specific socio-economic and healthcare conditions in each country

The diverse meteorology of Jezero crater over the first 250 sols of Perseverance on Mars

ASA’s Perseverance rover’s Mars Environmental Dynamics Analyzer is collecting data at Jezero crater, characterizing the physical processes in the lowest layer of the Martian atmosphere. Here we present measurements from the instrument’s first 250 sols of operation, revealing a spatially and temporally variable meteorology at Jezero. We find that temperature measurements at four heights capture the response of the atmospheric surface layer to multiple phenomena. We observe the transition from a stable night-time thermal inversion to a daytime, highly turbulent convective regime, with large vertical thermal gradients. Measurement of multiple daily optical depths suggests aerosol concentrations are higher in the morning than in the afternoon. Measured wind patterns are driven mainly by local topography, with a small contribution from regional winds. Daily and seasonal variability of relative humidity shows a complex hydrologic cycle. These observations suggest that changes in some local surface properties, such as surface albedo and thermal inertia, play an influential role. On a larger scale, surface pressure measurements show typical signatures of gravity waves and baroclinic eddies in a part of the seasonal cycle previously characterized as low wave activity. These observations, both combined and simultaneous, unveil the diversity of processes driving change on today’s Martian surface at Jezero crater

Universal spectral profile and dynamic evolution of muscle activation: A hallmark of muscle type and physiological state

The skeletal muscle is an integrated multicomponent system with complex dynamics of continuous myoelectrical activation of various muscle types across time scales to facilitate muscle coordination among units and adaptation to physiological states. To understand the multiscale dynamics of neuromuscular activity, we investigated spectral characteristics of different muscle types across time scales and their evolution with physiological states. We hypothesized that each muscle type is characterized by a specific spectral profile, reflecting muscle composition and function, that remains invariant over time scales and is universal across subjects. Furthermore, we hypothesized that the myoelectrical activation and corresponding spectral profile during certain movements exhibit an evolution path in time that is unique for each muscle type and reflects responses in muscle dynamics to exercise, fatigue, and aging. To probe the multiscale mechanism of neuromuscular regulation, we developed a novel protocol of repeated squat exercise segments, each performed until exhaustion, and we analyzed differentiated spectral power responses over a range of frequency bands for leg and back muscle activation in young and old subjects. We found that leg and back muscle activation is characterized by muscle-specific spectral profiles, with differentiated frequency band contribution, and a muscle-specific evolution path in response to fatigue and aging that is universal across subjects in each age group. The uncovered universality among subjects in the spectral profile of each muscle at a given physiological state, as well as the robustness in the evolution of these profiles over a range of time scales and states, reveals a previously unrecognized multiscale mechanism underlying the differentiated response of distinct muscle types to exercise-induced fatigue and aging

NETWORK PHYSIOLOGY OF INTER-MUSCULAR INTERACTIONS

Maddie Sayre1, Celeste Childs1, Libby Connolly1, Maddie Davis1, Quinlyn Shannehan1, Gabriela Simpson1, Sergi Garcia-Retortillo1, Plamen Ch Ivanov2. 1Wake Forest University, Winston-Salem, NC. 2Boston University, Boston, MA. BACKGROUND: Skeletal muscles continuously coordinate to facilitate a wide range of movements. Muscle fiber composition and timing of activation account for distinct muscle functions and dynamics necessary to fine tune muscle coordination, generate movements, and adapt to fatigue. Here we investigate how distinct muscle fiber types dynamically synchronize and integrate as a network across muscles in response to fatigue. METHODS: Fourteen healthy adults performed three maximal body weight squat tests until exhaustion. Electromyography (EMG) signals from the following muscles were recorded simultaneously during the entire protocol: left and right vastus lateralis (LegL and LegR); left and right erector spinae (BackL and BackR). We first obtained 10 time series of EMG band power for each muscle, representing the dynamics of different muscle fiber types. To investigate cross-frequency interactions among EMG frequency bands that occur as a result of synchronous modulation of their spectral amplitudes, we calculated the bivariate equal-time Pearson’s cross-correlation for each pair of EMG band power time series across all Leg and Back muscles. RESULTS: Different muscle fiber types dynamically synchronize their activity across muscles following distinct patterns of cross-frequency communication. Specifically, with progression of fatigue, same-type muscle subnetworks (LegL-LegR and BackL-BackR) exhibit statically significant (i) global decline in links strength (p \u3c 0.05) and (ii) increase in links strength stratification (p \u3c 0.03), while (iii) preserving the general functional form of the network profile. In contrast, sub-networks of different-type muscles (Leg-Back) exhibit significant (i) global increase in links strength (p \u3c 0.05) and (ii) decline in links strength stratification (p \u3c 0.02), while (iii) changing the functional form of the network profile. CONCLUSION: This work addresses inter-muscular interactions among rhythms of myoelectrical activation, corresponding to the activity of different type muscle fibers, across muscles in response to fatigue. This dynamic network approach can lead to the development of network-based markers that will break new ground in the study of multilevel inter-muscular interactions, and will provide new understanding of diverse exercise-related phenomena such as performance, fatigue or muscle injuries