“You could find a good or a bad provider, I would say you just have to go and see”: A qualitative study of the influence of perceptions of service quality on family planning service use in Burkina Faso

This qualitative study from Burkina Faso explores community-level perceptions of family planning (FP) service quality among FP users and non-users. It examines how perspectives on service quality may influence women’s motivation to seek modern methods from health facilities. For this study, twenty focus group discussions were undertaken with non-users and current users of modern FP including unmarried, sexually active women ages 15–19 and 20–24 and ever married women ages 15–24 and 25+ in Bobo Dioulasso and Banfora, Burkina Faso. The findings demonstrate that respondents prioritized a welcoming environment, positive provider-client exchanges, the full provision of information (especially about side-effects), a pain-free experience, a short waiting time, and privacy and confidentiality. Poor service quality did not, in general, reduce women’s demand or need to use a FP method. Some women who were reluctant to use formal health services used a non-facility-based method (calendar method, withdrawal, condoms or abstinence). Importantly, many unmarried, younger women and adolescents, who were more likely to be stigmatized by providers, exhibited agency by proactively seeking a method despite the potential for a negative experience. They prioritized their health and wellbeing over and above any interpersonal barriers they were likely to encounter. Incorporating strategies to improve the quality of FP services based on locally defined elements of quality should be a specific programmatic goal. These strategies can be identified through quality assessments employing a woman-centered lens. Women who visit facilities can be encouraged to share their positive experiences with their networks to improve community-level perspectives of facility quality. Improving service quality can attract new users, especially adolescents, and retain those who have already adopted a FP method. Through these multi-pronged actions, women’s (and community) expectations and experience of quality can improve. This, in turn, may lead to greater client satisfaction and associated higher FP prevalence

Landscape-based nutrient application in wheat and teff mixed farming systems of Ethiopia: farmer and extension agent demand driven approach

Introduction: Adapting fertilizer use is crucial if smallholder agroecosystems are to attain the sustainable development goals of zero hunger and agroecosystem resilience. Poor soil health and nutrient variability characterize the smallholder farming systems. However, the current research at the field scale does not account for nutrient variability across landscape positions, posing significant challenges for targeted nutrient management interventions. The purpose of this research was to create a demand-driven and co-development approach for diagnosing farmer nutrient management practices and determining landscape-specific (hillslope, mid-slope, and foot slope) fertilizer applications for teff and wheat. Method: A landscape segmentation approach was aimed to address gaps in farm-scale nutrient management research as well as the limitations of blanket recommendations to meet local nutrient requirements. This approach incorporates the concept of interconnected socio-technical systems as well as the concepts and procedures of co-development. A smart mobile app was used by extension agents to generate crop-specific decision rules at the landscape scale and forward the specific fertilizer applications to target farmers through SMS messages or print formats. Results and discussion: The findings reveal that farmers apply more fertilizer to hillslopes and less to mid- and foot slopes. However, landscape-specific fertilizer application guided by crop-specific decision rules via mobile applications resulted in much higher yield improvements, 23% and 56% at foot slopes and 21% and 6.5% at mid slopes for wheat and teff, respectively. The optimized net benefit per hectare increase over the current extension recommendation was $176 and$333 at foot slopes and $159 and$64 at mid slopes for wheat and teff (average of $90 and$107 for wheat and teff), respectively. The results of the net benefit-to-cost ratio (BCR) demonstrated that applying landscape-targeted fertilizer resulted in an optimum return on investment ($10.0 net profit per$1.0 investment) while also enhancing nutrient use efficiency across the three landscape positions. Farmers are now cognizant of the need to reduce fertilizer rates on hillslopes while increasing them on parcels at mid- and foot-slope landscapes, which have higher responses and profits. As a result, applying digital advisory to optimize landscape-targeted fertilizer management gives agronomic, economic, and environmental benefits. The outcomes results of the innovation also contribute to overcoming site-specific yield gaps and low nutrient use efficiency, they have the potential to be scaled if complementing innovations and scaling factors are integrated

"You could find a good or a bad provider, I would say you just have to go and see": A qualitative study of the influence of perceptions of service quality on family planning service use in Burkina Faso.

This qualitative study from Burkina Faso explores community-level perceptions of family planning (FP) service quality among FP users and non-users. It examines how perspectives on service quality may influence women's motivation to seek modern methods from health facilities. For this study, twenty focus group discussions were undertaken with non-users and current users of modern FP including unmarried, sexually active women ages 15-19 and 20-24 and ever married women ages 15-24 and 25+ in Bobo Dioulasso and Banfora, Burkina Faso. The findings demonstrate that respondents prioritized a welcoming environment, positive provider-client exchanges, the full provision of information (especially about side-effects), a pain-free experience, a short waiting time, and privacy and confidentiality. Poor service quality did not, in general, reduce women's demand or need to use a FP method. Some women who were reluctant to use formal health services used a non-facility-based method (calendar method, withdrawal, condoms or abstinence). Importantly, many unmarried, younger women and adolescents, who were more likely to be stigmatized by providers, exhibited agency by proactively seeking a method despite the potential for a negative experience. They prioritized their health and wellbeing over and above any interpersonal barriers they were likely to encounter. Incorporating strategies to improve the quality of FP services based on locally defined elements of quality should be a specific programmatic goal. These strategies can be identified through quality assessments employing a woman-centered lens. Women who visit facilities can be encouraged to share their positive experiences with their networks to improve community-level perspectives of facility quality. Improving service quality can attract new users, especially adolescents, and retain those who have already adopted a FP method. Through these multi-pronged actions, women's (and community) expectations and experience of quality can improve. This, in turn, may lead to greater client satisfaction and associated higher FP prevalence

Stability of Loop-Mediated Isothermal Amplification (LAMP) Reagents and its Amplification Efficiency on Crude Trypanosome DNA Templates

This study evaluated the stability of LAMP reagents when stored at 25C and 37C, and also assessed its detection efficiency on different DNA template preparations. Accordingly, LAMP using reagents stored at 25C and 37C amplified DNA of in vitro cultured T. b. brucei (GUTat 3.1) from day 1 to day 15 of reagent storage. There were no significant differences (P>0.05) in detection sensitivity of LAMP among the reagents stored at 25C, 37C and –20C (recommended storage temperature). LAMP using the reagents stored at above-mentioned temperatures amplified serially diluted DNAs (genomic DNA extracted by phenol-chloroform method, FTA card and hemolysed blood) of T. b. gambiense (IL2343) with high sensitivity. Reactions were conducted on the reagents stored from 1 day to 30 days. LAMP detection sensitivity was poor when fresh blood as DNA template was added directly into reactive solution. Results of this study demonstrated that LAMP has the potential to be used in field conditions for diagnosis of trypanosome infections without being affected by ambient temperatures of tropical and sub-tropical countries where trypanosomosis is endemic.http://wwwsoc.nii.ac.jp/jsvs/03_Journal/Journal-en.htm

Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes

We have developed two loop-mediated isothermal amplification (LAMP) assays for the detection of Theileria parva, the causative agent of East Coast fever (ECF), an economically important cattle disease in eastern, central and southern Africa. These assays target the polymorphic immunodominant molecule (PIM) and p150 LAMP genes. The primer set for each gene target consists of six primers, and each set recognises eight distinct regions on the target gene to give highly specific detection of T. parva. The detection limit of each primer set is 1 fg, which is equivalent to one copy of the PIM and p150 T. parva genes. These PIM and p150 LAMP primer sets amplify DNA of T. parva isolates from cattle and buffalo from different countries including Kenya, South Africa, Tanzania, Rwanda, Uganda and Burundi, indicating their ability to detect T. parva from different countries. With the advantages of simplicity, rapidity and cost effectiveness, these LAMP assays are good candidates for molecular epidemiology studies and for monitoring control programs in ECF-endemic, resource poor countries.http://www.elsevier.com/wps/find/journaldescription.cws_home/353/description#descriptio

Validated fertilizer use at landscape scale: demand driven approach in sorghum, wheat and teff mixed farming systems in Ethiopia: A Technical Report

Soil nutrient management is very critical to maximize crop yield and to maintain soil health for a sustainable productivity. Decline in soil fertility and soil quality, among other factors, are major constraints to the agricultural productivity and disfunction of environmental services (Bahr, 2015). In Ethiopia, soil nutrient mining and very less replenishment of organic and inorganic resources are the recurrent problems that resulted in soil nutrient depletion. Besides, severe topsoil erosion associated with steep slope cultivation made the country one of the highest nutrient depletion rates in Africa with 41, 6 and 26 kg ha-1yr-1 of nitrogen, phosphorus and potassium, respectively (Stoorvogel and Smaling, 1990). Soil nutrient balance assessments in central Ethiopia showed that nutrient losses even worsen and reached an amount of 122 kg N, 13 kg P and 82 kg K ha-1 per year-(Haileslassie et al., 2005). In addition to the poor nutrient and organic matter status, aluminum toxicity and phosphorous fixation are other constraints in Ethiopian soils apparent in pH less than 5.5 which enhances nutrient limitations and toxicity (Agegnehu and Amede, 2017; Agegnehu et al., 2006). The state of nutrient depletion entails context specific nutrient management and fertilizer applications