Growth Pattern of Pseudomonas aeruginosa in different wastewater media

Restaurant wastewater are a major cause of environmental pollution with the indiscriminate release of the effluent to the environment resulting in blocking of drainages and eutrophication thereby causing serious threat to aquatic life. The growth pattern of a dietary rich oily wastewater degrading bacteria strain, Pseudomonas aeruginosa in different wastewater media composed to mimic the possible constituents of restaurant wastewater was investigated in this study. There was noticeable microbial growth in the synthetic and domestic wastewaters after 48h while the detergent wastewater and heated oil-detergent wastewater did not support the strain's growth. The decrease in the fat content with a corresponding increase in the ash content after 120h was due to the test strain's metabolic activity, which is slightly higher in domestic wastewater than the heated oil-detergent solution. Also, the potassium (K+), Mg2+, Fe2+ and Ca2+ contents increased within the same period in both media except in the domestic wastewater where the Ca2+ content reduced. Protease enzyme activity (46.440mM/min) was considerably higher in the domestic wastewater than lipase (3.322mM/min) and amylase activity (14.244mM/min) after 72 hours of incubation. The pristine genetic properties of Pseudomonas aeruginosa altered when cultured in various wastewaters probably due to variation in the composition of the substrates

Screening of traditionally used Tanzanian medicinal plants for antifungal activity

Kisangau DP, Hosea KM, Lyaruu HVM, et al. Screening of traditionally used Tanzanian medicinal plants for antifungal activity. PHARMACEUTICAL BIOLOGY. 2009;47(8):708-716.Fungal infections represent a significant cause of morbidity and mortality especially in immunocompromised patients in the world today. Dichloromethane (DM) and aqueous (W) extracts of nine plants used traditionally for the treatment of fungal infections in Bukoba rural district in Tanzania were screened for antifungal activity against Candida albicans, Cryptococcus neoformans, and Aspergillus niger using agar well and disk diffusion methods. Dichloromethane extracts of Capparis erythrocorpos [CE] Isert (Capparaceae), Cussonia arborea [CA] Hochst. Ex A. Rich (Araliaceae), Drocaena steudneri [DS] Engl. (Dracaenaceae), Lannea schimperi [LS] (A. Rich) Engl. (Anacardiaceae), Rouvolfia vomitoria [RV] Afz (Apocynaceae), and Sapium ellipticum [SE] (Krauss) Pax (Euphorbiaceae) showed activity against all three fungi. Extracts of Rumex usambarensis [RU] (Dammer) Dammer (Polygonaceae) and Zehneria scabro [ZS] (L.f.) Sond. (Cucurbitaceae) had an activity limited to only one or two of the test organisms. Rhoicissus tridentata [RT] (L.Q Wild & Drum (Vitaceae) was the only plant without activity. Fractions of the active extracts CE, CA, DS, LS, and SE exhibited higher antifungal activity against one or more of the three fungi. Four compounds isolated from S. ellipticum also exhibited antifungal activity against one or more of the three fungi. The minimum inhibitory concentrations (MICs) and minimum fungicidal concentrations (MFCs), determined using the microplate assay method, ranged between 0.4 and 50.0 mu g/mL for crude extracts, 1.6 and 50.0 mu g/mL for semi-purified fractions, and 0.12 and 1.0 mu g/mL for pure compounds, as compared to 0.016-1.5 mu g/mL for fluconazole. We confirm the potential of traditionally used plants as a source of new drugs for treatment of fungal infections