Características fenotípicas e genotípicas de estirpes de Escherichia coli produtora de toxina Shiga (STEC) isoladas de bovinos no Estado do Paraná

Orientadora : Cyntia M. T. Fadel PichethCo-orientador: Emanuel Maltempi de SouzaDissertaçao (mestrado) - Universidade Federal do Paraná, Setor de Ciencias da Saúde, Programa de Pós-Graduaçao em Ciencias Farmaceuticas. Defesa: Curitiba, 2005Inclui bibliografiaÁrea de concentraçao: Análises clínica

Características fenotípicas e genotípicas de estirpes de Escherichia coli produtora de toxina Shiga (STEC) isoladas de bovinos no Estado do Paraná

Orientadora : Cyntia M. T. Fadel PichethCo-orientador: Emanuel Maltempi de SouzaDissertaçao (mestrado) - Universidade Federal do Paraná, Setor de Ciencias da Saúde, Programa de Pós-Graduaçao em Ciencias Farmaceuticas. Defesa: Curitiba, 2005Inclui bibliografiaÁrea de concentraçao: Análises clínica

Utilization of carbon sources by clinical isolates of Aeromonas

Bacteria in the genus Aeromonas are primarily aquatic organisms; however, some species can cause diseases in humans, ranging from wound infections to septicemia, of which diarrhea is the most common condition. The ability to use a variety of carbon substrates is advantageous for pathogenic bacteria. Therefore, we used Biolog GN2 microplates to analyze the ability of 103 clinical, predominantly diarrheal, isolates of Aeromonas to use various carbon sources and verified whether among the substrates metabolized by these strains there were some endogenous to the human intestine. Results indicate that Aeromonas present great diversity in the utilization of carbon sources, and that they preferentially use carbohydrates and amino acids as carbon sources. Among the carbon sources metabolized by Aeromonas in vitro, some were found to be components of intestinal mucin including aspartic acid, glutamic acid, L-serine, galactose, N-acetyl-glucosamine, and glucose, which were used by all strains tested. Additionally, mannose, D-serine, proline, threonine, and N-acetyl-galactosamine were used by several strains. The potential to metabolize substrates endogenous to the intestine may contribute to Aeromonasâ capacity to grow in and colonize the intestine. We speculate that this may help explain the ability of Aeromonas to cause diarrhea.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author