52 research outputs found
Reconstitution of Nup157 and Nup145N into the Nup84 Complex
About 30 different nucleoporins (Nups) constitute the nuclear pore complex. We have affinity-purified 28 of these nuclear pore proteins and identified new nucleoporin interactions by this analysis. We found that Nup157 and Nup170, two members of the large structural Nups, and the Gly-Leu-Phe-Gly nucleoporin Nup145N specifically co-purified with members of the Nup84 complex. In addition, Nup145N co-enriched during Nup157 purification. By in vitro reconstitution, we demonstrate that Nup157 and Nup145N form a nucleoporin subcomplex. Moreover, we show that Nup157 and Nup145N bind to the heptameric Nup84 complex. This assembly thus represents approximately one-third of all nucleoporins. To characterize Nup157 structurally, we purified and analyzed it by electron microscopy. Nup157 is a hollow sphere that resembles a clamp or a gripping hand. Thus, we could reconstitute an interaction between a large structural Nup, an FG repeat Nup, and a major structural module of the nuclear pore complex
Random coil chemical shifts for serine, threonine and tyrosine phosphorylation over a broad pH range
Anisotropic 'hairs' in string cosmology
In this letter we investigate whether the isotropy problem is naturally
solved in inflationary cosmologies inspired by string theory, so called
pre-big-bang cosmologies. We find that, in contrast to what happens in the more
common 'potential inflation' models, initial anisotropies do not decay during
pre-big-bang inflation.Comment: 4 pages, 1 figur
Thermophile 90S Pre-ribosome Structures Reveal the Reverse Order of Co-transcriptional 18S rRNA Subdomain Integration
The âbirthâ of the eukaryotic ribosome is preceded by RNA folding and processing reactions that depend on assembly factors and snoRNAs. The 90S (SSU-processome) is the earliest pre-ribosome structurally analyzed, which was suggested to assemble stepwise along the growing pre-rRNA from 5â>3â, but this directionality may not be accurate. Here, by analyzing the structure of series of novel 90S assembly intermediates isolated from Chaetomium thermophilum, we discover a reverse order of 18S rRNA subdomain incorporation. This revealed that large parts of the 18S rRNA 3â and central domains assemble first into the 90S, before the 5â domain is stably integrated. This final incorporation depends on a physical contact between a heterotrimer Enp2-Bfr2-Lcp1 recruited to the flexible 5â domain and Kre33, which reconstitutes the Kre33-Enp-Brf2-Lcp5 module on the compacted 90S pre-ribosome. Keeping the 5â domain temporarily segregated from the 90S scaffold could provide an extra time to complete the multifaceted 5â domain folding, which depends on a distinct set of snoRNAs and processing factors
Adiabatic perturbations in pre big bang models: matching conditions and scale invariance
At low energy, the four-dimensional effective action of the ekpyrotic model
of the universe is equivalent to a slightly modified version of the pre big
bang model. We discuss cosmological perturbations in these models. In
particular we address the issue of matching the perturbations from a collapsing
to an expanding phase in full generality. We show that, generically, one
obtains for the spectrum of scalar perturbations in the original pre big
model (with vanishing potential). When an exponential potential for the dilaton
is included, a scale invariant spectrum () of adiabatic scalar
perturbations is produced under very generic matching conditions, both in a
modified pre big bang and ekpyrotic scenario. We also derive general results
valid for power law scale factors matched to a radiation dominated era.Comment: 11 pages, 1 figure, revised version with small corrections to match
version in print. Results and conclusions unchange
Probing the nucleoporin FG repeat network defines structural and functional features of the nuclear pore complex
A new tool to probe the FG repeat network of the nuclear pore complex transport channel in vivo provides insight into the organization and functional features of the channel
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