Tea seed oil: Physicochemical profiling

A study was undertaken to evaluate the quality characteristic of tea (Camellia sinensis L.) seed oil. The oil content ranged between 20.84 and 21.90 per cent. Smoke point, iodine value, saponification value, calorific value, refractive index, oil density, oil colour and oil pH were in the range of 247.29 - 251.53 (oC), 82.74 - 85.65 (g I 100-1 g), 185.33 - 185.72 (mg KOH g-1), 6822.53-6891.05 (J per 100 g), 1.46 (at 40 0C), 0.92 - 0.94 (g per cm3), 4.45 - 4.47 (Y+5R) and 4.62 - 4.64, respectively. The oxidation parameters, i.e., peroxide value, ranged from 1.17 - 2.63 meq kg-1. The tea seed oil has PUFA/SFA ratio 0.82 - 1.31 closer to WHO recommended value. Besides, antioxidant activity in term of DPPH free radical scavenging activity ranged between 6.30 - 7.14 per cent, β-carotene 4.62 - 12.93 mg kg-1 and α-tocopherol 90.49 - 366.52 mg kg-1. Highest oleic acid content was found in TSS 1, whereas highest α-tocopherol was found in TS 557. The results open up the possibilities of extracting oil from these bi-clonal seed stocks, which will diversify the use of tea

Insights into the functionality of endophytic actinobacteria with a focus on their biosynthetic potential and secondary metabolites production

Abstract Endophytic actinobacteria play an important role in growth promotion and development of host plant by producing enormous quantities of novel bioactive natural products. In the present investigation, 169 endophytic actinobacteria were isolated from endospheric tissues of Rhynchotoechum ellipticum. Based on their antimicrobial potential, 81 strains were identified by 16rRNA gene analysis, which were taxonomically grouped into 15 genera. All identified strains were screened for their plant growth promoting attributes and, for the presence of modular polyketide synthases (PKSI, PKSII and nonribosomal peptide synthetase (NRPS) gene clusters to correlate the biosynthetic genes with their functional properties. Expression studies and antioxidant potential for four representative strains were evaluated using qRT-PCR and DPPH assay respectively. Additionally, six antibiotics (erythromycin, ketoconazole, fluconazole, chloramphenicol, rifampicin and miconazole) and nine phenolic compounds (catechin, kaempferol, chebulagic acid, chlorogenic acid, Asiatic acid, ferulic acid, arjunic acid, gallic acid and boswellic acid) were detected and quantified using UHPLC-QqQLIT-MS/MS. Furthermore, three strains (BPSAC77, 121 and 101) showed the presence of the anticancerous compound paclitaxel which was reported for the first time from endophytic actinobacteria. This study provides a holistic picture, that endophytic actinobacteria are rich bacterial resource for bioactive natural products, which has a great prospective in agriculture and pharmaceutical industries

Author Correction: Insights into the functionality of endophytic actinobacteria with a focus on their biosynthetic potential and secondary metabolites production

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