Visual observation to identify sexes in subspecies of adult Black Skimmers (Rynchops niger)

Identifying sexes in birds from visual observations could be a useful and inexpensive method. While sexual dichromatism and ornaments are readily used by observers, sexual size dimorphism can also be used to identify sexes in some bird species. This study assessed the applicability of visual observation of size differences to identify sexes in adult Black Skimmers (Rynchops niger). Black Skimmers do not have sexual dichromatism; however, Black Skimmer males are skeletally larger (6.7–31.7% depending on trait) and 33.3–37.5% heavier than females. The study focused on two subspecies: Amazonian (R. n. cinerascens) and South American (R. n. intercedens) Black Skimmers. Sex identified by visually observing size differences was consistent with the sex identified at specimen preparation from examining gonads (RGLMM = 0.996 ± 0.004). The identification of sexes from photographs using visual observation of size had a very high within- (RGLMM = 0.995 ± 0.001) and between- (RGLMM = 0.984 ± 0.002) observer repeatability. Non-invasive methods for identifying sex by visual observation may allow enhanced use of data from photographic datasets, citizen science projects, and surveys using direct observation or images

Using field photography to study avian moult

Methods to obtain moult data from wild birds have not changed much over the last century and most studies still depend on checking museum specimens or capturing birds. Here we assess the applicability of systematic field photography for detecting and scoring moult in adult Black Skimmers Rynchops niger from southern Brazil. Moult data extracted from photographs have a high within- (RGLMM = 0.98) and between-observer repeatability (RGLMM = 0.97) and show very good fit to current Underhill–Zucchini moult models (R2 = 0.75). Photography offers the advantages of being less invasive, requiring less equipment and human effort, being feasible in areas where captures may not be possible, and causing less disturbance, so enhancing the number of sampled individuals

Evaluation of biological activity of Turkish plants. Rapid screening for the antimicrobial, antioxidant, and acetylcholinesterase inhibitory potential by TLC bioautographic methods

Using thin-layer chromatography (TLC) bioautography, a total of 58 extracts from various organs (aerial parts, leaves, flowers, fruits, roots) of 16 Turkish plants were tested for their antibacterial, antifungal, acetylcholinesterase inhibitory, antioxidant, and radical scavenging activities. The hexane, CHCl3/CH2Cl2, water, and total MeOH extracts were used. No activity was observed against two Gram-negative bacteria (Escherichia coli and Pseudomonas aureginosa) and the yeast Candida albicans. However, 23 plant extracts, mostly the CHCl3/CH2Cl2 and H2O-solubles, inhibited the growth of all five Gram-positive bacteria tested, Micrococcus luteus, Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, and Staphylococcus epidermidis. Of the active extracts, the CHCl3-soluble of the roots of Putoria calabrica (L. fil) DC (Rubiaceae) displayed the highest antibacterial potential. The majority of the CHCl3/CH2Cl2 crude extracts also appeared to inhibit acetylcholinesterase on TLC plates at 100 µg/spot concentration. Particularly active samples were the middle polarity extracts (CHCl3/CH2Cl2) of the leaves of Rhododendron smirnovii Trautv., R. ponticum L., and R. ungernii Trautv. (Ericaceae). β-Carotene, β-carotene/linoleic acid mixture, and 2,2-diphenyl-l-pieryhydrazyl (DPPH) solutions sprayed onto TLC plates were used for detecting antioxidant and radical scavenging properties of the crude extracts. Antioxidant and radical scavenging activities were found to be predominant in highly polar extracts. The water-solubles of all Rhododendron (Ericaceae) and Phlomis (Lamiaceae) species presented the most significant activity

Skin temperature reveals the intensity of acute stress

Acute stress triggers peripheral vasoconstriction, causing a rapid, short-term drop in skin temperature in homeotherms. We tested, for the first time, whether this response has the potential to quantify stress, by exhibiting proportionality with stressor intensity. We used established behavioural and hormonal markers: activity level and corticosterone level, to validate a mild and more severe form of an acute restraint stressor in hens (Gallus gallus domesticus). We then used infrared thermography (IRT) to non-invasively collect continuous temperature measurements following exposure to these two intensities of acute handling stress. In the comb and wattle, two skin regions with a known thermoregulatory role, stressor intensity predicted the extent of initial skin cooling, and also the occurrence of a more delayed skin warming, providing two opportunities to quantify stress. With the present, cost-effective availability of IRT technology, this non-invasive and continuous method of stress assessment in unrestrained animals has the potential to become common practice in pure and applied research

Defining potentially conserved RNA regulons of homologous zinc-finger RNA-binding proteins

Glucose inhibition of gluconeogenic growth suppressor 2 protein (Gis2p) and zinc-finger protein 9 (ZNF9) are conserved yeast and human zinc-finger proteins. The function of yeast Gis2p is unknown, but human ZNF9 has been reported to bind nucleic acids, and mutations in the ZNF9 gene cause the neuromuscular disease myotonic dystrophy type 2. To explore the impact of these proteins on RNA regulation, we undertook a systematic analysis of the RNA targets and of the global implications for gene expression

Analysis of the volatile components of five Turkish Rhododendron species by headspace solid-phase microextraction and GC-MS (HS-SPME-GC-MS)

Volatile constituents of various solvent extracts (n-hexane, CH2Cl2, H2O) of 15 different organs (leaves, flowers, fruits) of five Rhododendron species (Ericaceae) growing in Turkey were trapped with headspace solid-phase microextraction (HS-SPME) technique and analyzed by GC-MS. A total of 200 compounds were detected and identified from organic extracts, while the water extracts contained only traces of few volatiles. The CH2Cl2 extract of the R. luteum flowers was found to exhibit the most diverse composition: 34 compounds were identified, with benzyl alcohol (16.6%), limonene (14.6%) and p-cymene (8.4%) being the major compounds. The CH2Cl2-solubles of R. x sochadzeae leaves contained only phenyl ethyl alcohol. This study indicated appreciable intra-specific variations in volatile compositions within the genus. Different anatomical parts also showed altered volatile profiles. This is the first application of HS-SPME-GC-MS on the volatiles of Rhododendron species

Molecular Systematics of the Fishing Bat Myotis (Pizonyx) vivesi

Phylogenetic reconstructions based on molecular data have shown recurrent morphological convergence during evolution of the species-rich genus Myotis. Species or groups of species with similar feeding strategies have evolved independently several times to produce remarkable similarities in external morphology. In this context, we investigated the contentious phylogenetic position of 1 of the 2 piscivorous bat species, Myotis vivesi, which was not included in previous molecular studies. This bat, endemic to the coasts and islands of the Gulf of California, Mexico, was long classified in its own genus, Pizonyx, because of its distinctive morphology. To reconstruct its phylogenetic origins relative to other Myotis, we sequenced the mitochondrial cytochrome-b gene of 2 M. vivesi and related vespertilionids. These outgroups included Pipistrellus subflavus, a member of the subgenus Perimyotis, sometimes classified within the genus Myotis. Unexpectedly, all reconstructions placed M. vivesi within a strongly supported clade including all other typical neotropical and Nearctic Myotis. This molecular phylogeny supports an endemic radiation of New World Myotis. Other Myotis species with similar adaptations to gaffing prey from the water surface present no close phylogenetic relationships with M. vivesi, indicating that such adaptations are convergences. On the other hand, P. subflavus is genetically as distant from the genus Myotis as from other Pipistrellus species, suggesting that generic rank to Perimyotis is warrante

Swath-ms: a new data independent acquisition LC-MS methodology for quantitative complete proteome analysis

Comunicaciones a congreso

Secondary metabolites of Phlomis viscosa and their biological activities

Further phytochemical studies on the aerial parts of Phlomis viscosa (Lamiaceae) led to the isolation of 24 compounds: 3 iridoid glycosides, 10 phenylethanoid glycosides, a megastigmane glycoside and a hydroquinone glycoside, as well as 2 lignan glucosides and 7 neolignan glucosides, 1 of which is new (17b). Compound 17b was obtained as a minor component of an inseparable mixture (2:1) of 2 neolignan glucosides (17a/b), and characterized as 3',4-O-dimethylcedrusin 9-O-b -glucopyranoside. Full NMR data of the known 8-O-4' neolignan glucoside, erythro-1-(4-O-b-glucopyranosyl-3-methoxyphenyl)- 2-{2-methoxyl-4-[1-(E)-propene-3-ol]-phenoxyl}-propane-1,3-diol (18) are also reported. All isolated compounds were screened for cell growth inhibition versus 3 tumor cell lines (MCF7, NCI-H460, and SF-268) and several phenylethanoid glycosides were found to possess weak antitumoral activity. The phenylethanoid glycosides were also evaluated for their free radical (DPPH) scavenging, antibacterial and antifungal activities. The free radical (DPPH) scavenging activities of verbascoside (4), isoacteoside (5), forsythoside B (10), myricoside (13) and samioside (14) were found to be comparable to that of dl-a -tocopherol. Compounds 4, 5, 10 and 14 (MIC: 500 m g/mL) as well as Leucosceptoside A (8) and 13 (MIC:1000 m g/mL) showed very weak activity against Gram (+) bacteria