Human prostate sphere-forming cells represent a subset of basal epithelial cells capable of glandular regeneration in vivo.

BackgroundProstate stem/progenitor cells function in glandular development and maintenance. They may be targets for tumor initiation, so characterization of these cells may have therapeutic implications. Cells from dissociated tissues that form spheres in vitro often represent stem/progenitor cells. A subset of human prostate cells that form prostaspheres were evaluated for self-renewal and tissue regeneration capability in the present study.MethodsProstaspheres were generated from 59 prostatectomy specimens. Lineage marker expression and TMPRSS-ERG status was determined via immunohistochemistry and fluorescence in situ hybridization (FISH). Subpopulations of prostate epithelial cells were isolated by cell sorting and interrogated for sphere-forming activity. Tissue regeneration potential was assessed by combining sphere-forming cells with rat urogenital sinus mesenchyme (rUGSM) subcutaneously in immunocompromised mice.ResultsProstate tissue specimens were heterogeneous, containing both benign and malignant (Gleason 3-5) glands. TMPRSS-ERG fusion was found in approximately 70% of cancers examined. Prostaspheres developed from single cells at a variable rate (0.5-4%) and could be serially passaged. A basal phenotype (CD44+CD49f+CK5+p63+CK8-AR-PSA-) was observed among sphere-forming cells. Subpopulations of prostate cells expressing tumor-associated calcium signal transducer 2 (Trop2), CD44, and CD49f preferentially formed spheres. In vivo implantation of sphere-forming cells and rUGSM regenerated tubular structures containing discreet basal and luminal layers. The TMPRSS-ERG fusion was absent in prostaspheres derived from fusion-positive tumor tissue, suggesting a survival/growth advantage of benign prostate epithelial cells.ConclusionHuman prostate sphere-forming cells self-renew, have tissue regeneration capability, and represent a subpopulation of basal cells

Effect of Generating a Semantic Prime: The Impact of Age and Cognitive Impairment

Semantic priming studies, and studies of the generation effect (GE), have provided evidence to support either a structural or functional breakdown of semantic memory in dementia. To determine whether the act of generating an item enhanced its activation in semantic memory, young, elderly, and demented subjects participated in a semantic priming/generation effect paradigm. Response times were recorded for semantically related and unrelated targets presented after either read or generated word pair cues. Results suggested that generating information held little benefit for young or demented subjects in the related condition, although elderly subjects benefited more from generating information than from reading it. Results and implications or theories of dementia and its treatment will be discussed

Tinnitus as a Measure of Salicylate Toxicity in the Overdose Setting

Introduction: The development of tinnitus and/or hearing loss (THL) in patients receiving chronic salicylate therapy has been demonstrated. However, to date, little scientific data validates this relationship in the large single overdose setting.Objective: To correlate salicylate levels in patients with the subjective complaint of THL, following an acute salicylate overdose.Methods: A retrospective chart review of cases of acute salicylate toxicity and THL reported to the Illinois Poison Control Center (IPC) from 2001-2002 was performed. Data abstracted included age, gender, ingestion time, salicylate levels, and arterial blood gases.Results: Ninety-nine cases of THL were reviewed and analyzed with mean age of 23.7 years (SD: 10.9), 30.3% male, and 82.2% intentional overdoses. The average dose ingested was 20.0 grams (SD:20.2) and the mean time from ingestion to medical care was 12.4 hours (SD: 11.1). The mean initial ASA level was 48.3 mg/dl (SD: 16.4) with 86.9% having initial level ≥ 30mg/dl and 40.4% ≥ 50 mg/dl. 85.9% of cases presented to the hospital with their ASA level at or past peak. The mean pH was 7.45, pO2 = 108, pCO2 = 28.0, and HCO3 = 19.9.Conclusion: In this limited study, 85.9% of patients presenting with tinnitus and/or hearing loss following a single salicylate ingestion had initial salicylate levels at or past their peak and 86.9% were in the toxic range

Attention In Aphasia As Revealed by Event-Related Potentials: A Preliminary Investigation

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A Topographic Event-Related Potential Analysis of the Attention Deficit for Auditory Processing in Aphasia

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Discourse and identity in a corpus of lesbian erotica

This article uses corpus linguistic methodologies to explore representations of lesbian desires and identities in a corpus of lesbian erotica from the 1980s and 1990s. We provide a critical examination of the ways in which “lesbian gender,” power, and desire are represented, (re-)produced, and enacted, often in ways that challenge hegemonic discourses of gender and sexuality. By examining word frequencies and collocations, we critically analyze some of the themes, processes, and patterns of representation in the texts. Although rooted in linguistics, we hope this article provides an accessible, interdisciplinary, and timely contribution toward developing understandings of discursive practices surrounding gender and sexuality

Galaxies undergoing ram-pressure stripping: the influence of the bulge on morphology and star formation rate

We investigate the influence of stellar bulges on the star formation and morphology of disc galaxies that suffer from ram pressure. Several tree-SPH (smoothed particle hydrodynamics) simulations have been carried out to study the dependence of the star formation rate on the mass and size of a stellar bulge. In addition, different strengths of ram pressure and different alignments of the disc with respect to the intra-cluster medium (ICM) are applied. As claimed in previous works, when ram pressure is acting on a galaxy, the star formation rate (SFR) is enhanced and rises up to four times with increasing ICM density compared to galaxies that evolve in isolation. However, a bulge suppresses the SFR when the same ram pressure is applied. Consequently, fewer new stars are formed because the SFR can be lowered by up to 2 M_sun/yr. Furthermore, the denser the surrounding gas, the more inter-stellar medium (ISM) is stripped. While at an ICM density of 10^-28 g/cm^3 about 30% of the ISM is stripped, the galaxy is almost completely (more than 90%) stripped when an ICM density of 10^-27 g/cm^3 is applied. But again, a bulge prevents the stripping of the ISM and reduces the amount being stripped by up to 10%. Thereby, fewer stars are formed in the wake if the galaxy contains a bulge. The dependence of the SFR on the disc tilt angle is not very pronounced. Hereby a slight trend of decreasing star formation with increasing inclination angle can be determined. Furthermore, with increasing disc tilt angles, less gas is stripped and therefore fewer stars are formed in the wake. Reducing the disc gas mass fraction results in a lower SFR when the galaxies evolve in vacuum. On the other hand, the enhancement of the SFR in case of acting ram pressure is less pronounced with increasing gas mass fraction. Moreover, the fractional amount of stripped gas does not depend on the gas mass fraction.Comment: 11 pages, 18 figure

emm typing and validation of provisional M types for group A streptococci.

This report discusses the following issues related to typing of group A streptococci (GAS): The development and use of the 5' emm variable region sequencing (emm typing) in relation to the existing serologic typing system; the designation of emm types in relation to M types; a system for validation of new emm types; criteria for validation of provisional M types to new M-types; a list of reference type cultures for each of the M-type or emm-type strains of GAS; the results of the first culture exchange program for a quality control testing system among the national and World Health Organization collaborating centers for streptococci; and dissemination of new approaches to typing of GAS to the international streptococcal community

Isomorphisms of Brin-Higman-Thompson groups

Let $m, m', r, r',t, t'$ be positive integers with $r, r' \ge 2$. Let $L_r$ denote the ring that is universal with an invertible $1 \times r$ matrix. Let $M_m(L_r^{\otimes t})$ denote the ring of $m \times m$ matrices over the tensor product of $t$ copies of $L_r$. In a natural way, $M_m(L_r^{\otimes t})$ is a partially ordered ring with involution. Let $PU_m(L_r^{\otimes t})$ denote the group of positive unitary elements. We show that $PU_m(L_r^{\otimes t})$ is isomorphic to the Brin-Higman-Thompson group $t V_{r,m}$; the case $t =1$ was found by Pardo, that is, $PU_m(L_r)$ is isomorphic to the Higman-Thompson group $V_{r,m}$. We survey arguments of Abrams, \'Anh, Bleak, Brin, Higman, Lanoue, Pardo, and Thompson that prove that $t' V_{r',m'} \cong tV_{r,m}$ if and only if $r' = r$, $t'=t$ and $\gcd(m',r'-1) = \gcd(m,r-1)$ (if and only if $M_{m'}(L_{r'}^{\otimes t'})$ and $M_m(L_r^{\otimes t})$ are isomorphic as partially ordered rings with involution).Comment: 24 page