Effect of Bacterial Adsorption on Low Frequency Electrical Properties of Clean Quartz Sands and Iron-Oxide Coated Sands

Low frequency electrical measurements (0.1-1000 Hz) were conducted to investigate the adsorption effect of Pseudomonas aeruginosa cells onto clean quartz sands and iron-oxide coated sands. The clean quartz sands showed a gradual increase in the microbial adsorption to mineral grains, concurrent with an increase of 13% in the imaginary conductivity component (σ″). However, iron-oxide coated sands (20-100% by weight) showed a rapid increase in microbial adsorption with σ″ reaching a maximum of 37% for the 80-100% iron coated sands. No significant changes were observed in the real conductivity component (σ′) due to microbial adsorption. A power law dependency was observed between the adsorbed cells and σ″. We suggest that the polarization results from the increase in the surface roughness and surface area of the grain due to bacteria sorption. These results suggest that low frequency electrical measurements can play an important role in assessing microbial transport in subsurface environments

Sensitivity of Geoelectrical Measurements to the Presence of Bacteria in Porous Media

We investigated the sensitivity of low-frequency electrical measurements (0.1-1000 Hz) to (1) microbial cell density, (2) live and dead cells, and (3) microbial attachment onto mineral surfaces of clean quartz sands and iron oxide-coated sands. Three strains of Pseudomonas aeruginosa PAO1 (wild type and rhlA and pilA mutant) with different motility and attachment properties were used. Varying concentrations of both live and dead cells of P. aeruginosa wild type in sand columns showed no effect on the real conductivity component (σ′). However, the imaginary conductivity component (σ″) increased linearly with increasing concentrations of live cells in sand columns, whereas minimal changes were observed with different concentrations of dead cells. A strong power law relationship was observed between σ″ and the number of cells adsorbed onto sand grain surfaces with the rhlA mutant of P. aeruginosa displaying a higher power law exponent compared to the wild type and pilA mutant. In addition, power law exponents were greater in columns with iron oxide-coated sands compared to clean quartz sands. Minimal changes were observed on the σ′ due to the attachment of P. aeruginosa cells onto sands. We relate the measured low-frequency electrical responses to (1) the distinct electrical properties of live cells and (2) the density of cells attached to mineral surfaces enhancing the surface roughness of sand grains and hence the polarization response. The information obtained from this study enhances our interpretation of microbially induced geoelectrical responses in biostimulated geologic media and may have implications for microbial transport studies

Microbial Growth and Biofilm Formation in Geologic Media Is Detected with Complex Conductivity Measurements

Complex conductivity measurements (0.1-1000 Hz) were obtained from biostimulated sand-packed columns to investigate the effect of microbial growth and biofilm formation on the electrical properties of porous media. Microbial growth was verified by direct microbial counts, pH measurements, and environmental scanning electron microscope imaging. Peaks in imaginary (interfacial) conductivity in the biostimulated columns were coincident with peaks in the microbial cell concentrations extracted from sands. However, the real conductivity component showed no discernible relationship to microbial cell concentration. We suggest that the observed dynamic changes in the imaginary conductivity (σ″) arise from the growth and attachment of microbial cells and biofilms to sand surfaces. We conclude that complex conductivity techniques, specifically imaginary conductivity measurements are a proxy indicator for microbial growth and biofilm formation in porous media. Our results have implications for microbial enhanced oil recovery, CO2 sequestration, bioremediation, and astrobiology studies

Field-Scale Observations of a Transient Geobattery Resulting from Natural Attenuation of a Crude Oil Spill

We present evidence of a geobattery associated with microbial degradation of a mature crude oil spill. Self-potential measurements were collected using a vertical array of nonpolarizing electrodes, starting at the land surface and passing through the smear zone where seasonal water table fluctuations have resulted in the coating of hydrocarbons on the aquifer solids. These passive electrical potential measurements exhibit a dipolar pattern associated with a current source. The anodic and cathodic reactions of this natural battery occur below and above the smear zone, respectively. The smear zone is characterized by high magnetic susceptibility values associated with the precipitation of semiconductive magnetic iron phase minerals as a by-product of biodegradation, facilitating electron transfer between the anode and the cathode. This geobattery response appears to have a transient nature, changing on a monthly scale, probably resulting from chemical and physical changes in subsurface conditions such as water table fluctuations

Evidence for Microbial Enhanced Electrical Conductivity in Hydrocarbon-Contaminated Sediments

Bulk electrical conductivity of sediments during microbial mineralization of diesel was investigated in a mesoscale laboratory experiment consisting of biotic contaminated and uncontaminated columns. Population numbers of oil degrading microorganisms increased with a clear pattern of depth zonation within the contaminated column not observed in the uncontaminated column. Microbial community structure determined from ribosomal DNA intergenic spacer analysis showed a highly specialized microbial community in the contaminated column. The contaminated column showed temporal increases in bulk conductivity, dissolved inorganic carbon, and calcium, suggesting that the high bulk conductivity is due to enhanced mineral weathering from microbial activity. The greatest change in bulk conductivity occurred in sediments above the water table saturated with diesel. Variations in electrical conductivity magnitude and microbial populations and their depth distribution in the contaminated column are similar to field observations. The results of this study suggest that geophysical methodologies may potentially be used to investigate microbial activity

In-situ Apparent Conductivity Measurements and Microbial Population Distribution at a Hydrocarbon-Contaminated Site

We investigated the bulk electrical conductivity and microbial population distribution in sediments at a site contaminated with light nonaqueous-phase liquid (LNAPL). The bulk conductivity was measured using in-situ vertical resistivity probes; the most probable number method was used to characterize the spatial distribution of aerobic heterotrophic and oil-degrading microbial populations. The purpose of this study was to assess if high conductivity observed at aged LNAPL-impacted sites may be related to microbial degradation of LNAPL. The results show higher bulk conductivity coincident with LNAPL-impacted zones, in contrast to geoelectrical models that predict lower conductivity in such zones. The highest bulk conductivity was observed to be associated with zones impacted by residual and free LNAPL. Data from bacteria enumeration from sediments close to the resistivity probes show that oil-degrading microbes make up a larger percentage (5-55%) of the heterotrophic microbial community at depths coincident with the higher conductivity compared to ∼5% at the uncontaminated location. The coincidence of a higher percentage of oil-degrading microbial populations in zones of higher bulk conductivity suggests that the higher conductivity in these zones may result from increased fluid conductivity related to microbial degradation of LNAPL, consistent with geochemical studies that suggest that intrinsic biodegradation is occurring at the site. The findings from this study point to the fact that biogeochemical processes accompanying biodegradation of contaminants can potentially alter geoelectrical properties of the subsurface impacted media

Engineering bacterial competitiveness and persistence in the phytosphere.

Several tactics exist to improve the survival of an introduced microorganism of interest in the plant environment. One, derived from studies on the Agrobacterium-plant interaction and the role of opines in this interaction, proposes to promote growth of the inoculant in the plant environment via the establishment of a bias in the rhizosphere. It is supported by the occurrence of natural biases, such as those generated by opine-like molecules, by calestegins, or by mimosine. Opine-mediated biases have allowed several investigators to favor the growth of opine-degrading bacteria or communities under sterile or axenic environments or in microcosms mimicking near field conditions. Another way to favor a given microbe consists in impeding growth of competing microorganisms. Experiments performed using detergent or bacteriostatic agents as amendments under field or near field conditions yielded promising results. Research perspectives for engineering plant-microbe interactions also include specific engineering of predation and strategies designed to interfere with some of the signals perceived by the microbes, provided these signals control the expression of functions central to microbial fitness. In this respect, quorum-sensing signal molecules, such as N-acyl-homoserine lactones, may be valuable targets for the development of biocontrol agents and procedures

Inositol Catabolism, a Key Pathway in Sinorhizobium meliloti for Competitive Host Nodulation▿ †

The nitrogen-fixing symbiont of alfalfa, Sinorhizobium meliloti, is able to use myo-inositol as the sole carbon source. Putative inositol catabolism genes (iolA and iolRCDEB) have been identified in the S. meliloti genome based on their similarities with the Bacillus subtilis iol genes. In this study, functional mutational analysis revealed that the iolA and iolCDEB genes are required for growth not only with the myo-isomer but also for growth with scyllo- and d-chiro-inositol as the sole carbon source. An additional, hypothetical dehydrogenase of the IdhA/MocA/GFO family encoded by the smc01163 gene was found to be essential for growth with scyllo-inositol, whereas the idhA-encoded myo-inositol dehydrogenase was responsible for the oxidation of d-chiro-inositol. The putative regulatory iolR gene, located upstream of iolCDEB, encodes a repressor of the iol genes, negatively regulating the activity of the myo- and the scyllo-inositol dehydrogenases. Mutants with insertions in the iolA, smc01163, and individual iolRCDE genes could not compete against the wild type in a nodule occupancy assay on alfalfa plants. Thus, a functional inositol catabolic pathway and its proper regulation are important nutritional or signaling factors in the S. meliloti-alfalfa symbiosis