6 research outputs found
Tissue distribution of a plasmid DNA encoding Hsp65 gene is dependent on the dose administered through intramuscular delivery
- Publication venue
- BioMed Central
- Publication date
- 01/01/2006
- Field of study
In order to assess a new strategy of DNA vaccine for a more complete understanding of its action in immune response, it is important to determine the in vivo biodistribution fate and antigen expression. In previous studies, our group focused on the prophylactic and therapeutic use of a plasmid DNA encoding the Mycobacterium leprae 65-kDa heat shock protein (Hsp65) and achieved an efficient immune response induction as well as protection against virulent M. tuberculosis challenge. In the present study, we examined in vivo tissue distribution of naked DNA-Hsp65 vaccine, the Hsp65 message, genome integration and methylation status of plasmid DNA. The DNA-Hsp65 was detectable in several tissue types, indicating that DNA-Hsp65 disseminates widely throughout the body. The biodistribution was dose-dependent. In contrast, RT-PCR detected the Hsp65 message for at least 15 days in muscle or liver tissue from immunized mice. We also analyzed the methylation status and integration of the injected plasmid DNA into the host cellular genome. The bacterial methylation pattern persisted for at least 6 months, indicating that the plasmid DNA-Hsp65 does not replicate in mammalian tissue, and Southern blot analysis showed that plasmid DNA was not integrated. These results have important implications for the use of DNA-Hsp65 vaccine in a clinical setting and open new perspectives for DNA vaccines and new considerations about the inoculation site and delivery system
Stem cells therapy for cardiovascular repair in ischemic heart disease: How to predict and secure optimal outcome?
- Author
- A Hirsch
- AB Mathiasen
- ACP Diederichsen
- B Assmus
- B Assmus
- B Puissant
- BE Strauer
- BE Strauer
- BE Strauer
- C Martinez
- C Rosada
- CE Veltman
- D Mocini
- D Zohlnhöfer
- DG Katritsis
- DG Katritsis
- DW Losordo
- E Martin-Rendon
- EC Perin
- EC Perin
- EM Horwitz
- F Baldazzi
- F Fernandez-Aviles
- F Kuethe
- FH Seeger
- FM Watt
- GP Meyer
- H Ahmadi
- HF Tze
- HJ Kang
- HV Huikuri
- I Bochev
- I Gunaseeli
- J Kajstura
- J Kastrup
- J Kastrup
- J Lough
- J Ramshorst Van
- JK Fraser
- JM Hare
- JS Wang
- JY Li
- K Lin
- K Lunde
- K Takahashi
- K Yusa
- KC Wollert
- M Gyongyosi
- M Haack-Sorensen
- M Haack-Sorensen
- M Haack-Sørensen
- M Hendrikx
- M Mohyeddin-Bonab
- M Tendera
- M Yousef
- MA Laflamme
- MF Pittenger
- ML Silva da
- P Menasche
- PA Zuk
- PC Smits
- PR Vulliet
- RH Lee
- RS Ripa
- RS Ripa
- S Chen
- S Erbs
- S Fernandes
- S Fuchs
- S Heydarkhan-Hagvall
- S Janssens
- S Makino
- S Yamanaka
- SA Kuznetsov
- SL Chen
- SLMA Beeres
- SLMA Beeres
- SLMA Beeres
- T Egeland
- T Freyman
- T Murohara
- V Schachinger
- V Schachinger
- V Schachinger
- Y Wang
- Z Izsvak
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
Effects of Micro-environmental pH of Liposome on Chemical Stability of Loaded Drug
- Author
- A Khanal
- AA Metwally
- AA Metwally
- AB Kunnumakkara
- C Li
- DB Vieira
- G Zhang
- GT Noble
- HE Hassan
- J Zou
- JM Metselaar
- L Sercombe
- M Mehanny
- M Ying
- M Ying
- MA Collier
- MM Yallapu
- N Vijaykumar
- Na Fu
- PP Nahar
- Q Peng
- Q Peng
- Q Peng
- Q Peng
- Qiang Peng
- RS Rosada
- S Grijalvo
- S Lazzari
- S Zununi Vahed
- Shu Zhang
- T Chang
- TX Nguyen
- V Ayala
- V Forster
- WD Wu
- X Huang
- X Wang
- X Wei
- Xiao-Ru Shao
- Xiao-Xiao Cai
- XQ Wei
- XR Shao
- Xue-Qin Wei
- YC Kuo
- YJ Wang
- Yun-Feng Lin
- Z Yan
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
Adipocytes and Osteoblasts from Human Adipose Tissue Mesenchymal Stem Cells for the Production of Compatible and Safe Biomaterial Crucial in Cosmetic, Reconstructive, and Regenerative Medicine
- Author
- A Banas
- A Campenhout Van
- AH Undale
- AJ Quick
- AN Georgiadis
- BE Wisse
- BM Strem
- C Bari De
- C Rosada
- CK Rebellatto
- CT Gomillion
- CT Tran
- DL Stocum
- DZ Zhang
- F Jakob
- FJ O’Brien
- G Efstratiadis
- G Fantuzzi
- G Isaacson
- G Lidong
- H Lin
- H Mizuno
- H Mizuno
- HS Goodwin
- JA Pawitan
- JJ Minguell
- K Bieback
- K Mori
- K Sudo
- KS Park
- L Casteilla
- L Kucerova
- M Abedin
- MEJ Reinders
- MS Tsai
- O Koc
- O Parolini
- OK Lee
- P Niemeyer
- PA Janmey
- PA Zuk
- Q Wang
- Q Ye
- R Ogawa
- R Yanez
- RG Johnson
- RS Tuan
- S Jono
- S Sathishkumar
- T Stompor
- TA Einhorn
- TM Liu
- TT Tran
- W Xu
- YC Wu
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
ATLAS: Technical proposal for a general-purpose p p experiment at the Large Hadron Collider at CERN
- Author
- Abdinov OB
- Abolins M
- Abramowicz H
- Aderholz M
- Agehed K
- Agnvall S
- Ajaltouni Z
- Akesson T
- Albiol F
- Aleksandrov I
- Alekseev G
- Alexander G
- Alexanian H
- Alexiev D
- Alfino M
- Aliev FM
- Aliev RM
- Allport PP
- Almehed S
- Aloisio A
- Alviggi MG
- Amako K
- Ambrosini G
- Amelin DV
- Ammosov VV
- Amorim A
- Anderson K
- Andrle J
- Angelini C
- Anghinolfi F
- Anosov V
- Apsey RQ
- Apsimon R J
- Arai Y
- Ardelean J
- Arik E
- Armitage J
- Armstrong R
- Armstrong WW
- Arnault C
- Arneodo M
- Arsenescu R
- Artamonov A
- Arvidson A
- Asai M
- Astbury A
- Astvatsaturov A
- Aubert B
- Auge E
- Autiero D
- Axen D
- Azhgirei L
- Azuelos G
- Bacci C
- Badaud F
- Bagnaia P
- Bailey DC
- Baines J T
- Bakker FE
- Ban J
- Bannikov A
- Baranov SA
- Baranov SP
- Barberio E
- Barberis D
- Barrand G
- Barreira G
- Barreiro F
- Basa S
- Bashindzhagian GL
- Basiladze SG
- Batley JR
- Batrakov A
- Battistoni G
- Baubillier M
- Baynham D E
- Bazan A
- Baze JM
- Beaudoin G
- Beaugiraud B
- Beck GA
- Becks KH
- Bee C
- Beer G
- Behrends S
- Bella G
- Bellettini G
- Bellini G
- Belov M
- Benary O
- Benlloch JM
- Bensinger JR
- Berger EL
- Berglund S
- Bergman R
- Bergsma F
- Beringer J
- Bernard R
- Bezamat J
- Bhadra S
- Biagi SF
- Bibby JH
- Bignall P
- Billoir P
- Bilokon H
- Bingefors N
- Bintinger D
- Birney P
- Birol I
- Blair GA
- Blair R
- Blaj C
- Blanchot G
- Blanquart L
- Blocker C
- Blocki J
- Blouzon F
- Blucher E
- Blum W
- Bobbink GJ
- Bock R
- Bogolyubsky MYu
- Bogush AA
- Bohm C
- Bohm J
- Boldea V
- Bondarenko V
- Boniface J
- Bonino R
- Bonzom V
- Boos E
- Booth CN
- Booth PSL
- Borer K
- Borisov AA
- Borisov O
- Bos K
- Bosi F
- Bosman M
- Boterenbrood H
- Botner O
- Botterill D R
- Bouchel M
- Bouhemaid N
- Bourdinaud M
- Bowcock TJV
- Boyko I
- Bozhko NI
- Bozzo M
- Brenner R
- Breskin A
- Breton D
- Brette P
- Brettel H
- Breuker H
- Brisson JC
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- Brodbeck TJ
- Broer W
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- Brossard M
- Brouwer C
- Bruncko D
- Bryzgalov VV
- Buchholz P
- Budagov Yu
- Bugge L
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- Burckhart HJ
- Burnett TH
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- Caloba LP
- Calvet D
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- Camarri P
- Cambiaghi M
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- Candlin DJ
- Canton B
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- Carlson P
- Carney RE
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- Castillo A
- Cattaneo PW
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- Cavasinni V
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- Cesaroni F
- Cesura G
- Cevenini F
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- Charlton DG
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- Chechik R
- Chekhtman A
- Chekulaev SV
- Chelkov G
- Chen J
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- Chernichenko SK
- Chernyatin V
- Chevaleyre JC
- Chevalier L
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- Chiarella V
- Chiefari G
- Chikovani L
- Chiladze B
- Chilingarov A
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- Chlachidze G
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- Chmil V
- Chochula P
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- Chouridou S
- Christophorou G
- Chudakov EA
- Chuntonov A
- Ciapetti G
- Cicek Z
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- Edgecock T R
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- Munday DJ
- Mur M
- Muravev S
- Murray P
- Murray W J
- Murtagh MJ
- Musico P
- Myagkov AG
- Nacasch R
- Nadtochy A
- Nagy E
- Nakagawa Y
- Napier A
- Napolitano M
- Nayman P
- Nemecek S
- Nessi M
- Neuheimer E
- Nevsky P
- Newcomer FM
- Nichols A
- Nickerson RB
- Nicoleau S
- Nielsen BS
- Nikolenko M
- Nisati A
- Nodulman LJ
- Noffz KH
- Nomachi M
- Noppe JM
- Norton P R
- Nozaki M
- Nozdrin A
- Nurdan K
- Nuti M
- Nygard E
- Nygren D
- O'Connor P
- O'Neale SW
- O'Neill M
- O'Shea V
- Oakham G
- Oberlack H
- Obudovsky V
- Ogren H
- Oh SH
- Ohska TK
- Ohsugi T
- Oliver J
- Olivetto C
- Olness FI
- Olsen WC
- Olshevsky A
- Olszowska J
- Onions C
- Onofre A
- Onuchin VA
- Oram C
- Oren Y
- Orestano D
- Orr RS
- Orteu S
- Osborne LS
- Osculati B
- Ostapchuk A
- Ota J
- Owen D
- Pabot Y
- Pacheco A
- Padilla C
- Paige F
- Pal T
- Palamara O
- Palchetti L
- Pallin D
- Pan YB
- Panareo M
- Panin V
- Pantea D
- Papadopoulou T
- Paradiso JA
- Parker MA
- Parker SI
- Parodi F
- Parrour G
- Parsons J
- Pascual J
- Pastore F
- Patoux A
- Patricelli S
- Pavlenko S
- Payne B T
- Peach K J
- Pedrini D
- Pelfer PG
- Pennacchio E
- Pentney JM
- Pepe-Altarelli M
- Perasso L
- Perdahci Z
- Perera VJO
- Perini L
- Perlas JA
- Perrin E
- Peshekhonov V
- Petrera S
- Petridou C
- Petroff P
- Petrolo E
- Phillips P W
- Pier S
- Pikna M
- Pilcher J
- Pinfold JL
- Pinhao J
- Pleskach AV
- Pless IA
- Plothow-Besch H
- Poffenberger P
- Poggioli L
- Poirot S
- Pokrovsky N
- Polesello G
- Pols CLA
- Polychronakos V
- Pontecorvo L
- Pope BG
- Popov AI
- Popov L
- Poppleton A
- Pose R
- Pospisil S
- Pouit L
- Poulard G
- Povinec P
- Pretzl K
- Price LE
- Price M
- Primavera M
- Pritchard T W
- Protopopescu S
- Protopopov YuM
- Proudfoot J
- Pukhov O
- Putzer A
- Qian Z
- Radeka V
- Raffaelli M
- Ragusa F
- Rahm DC
- Raine C
- Ratoff PN
- Raymond M
- Rees DL
- Reichold A
- Reinhall P
- Reinmuth G
- Reinsch A
- Renardy JF
- Renk B
- Rensch B
- Renton PB
- Renzoni G
- Rescia S
- Revel D
- Rewiersma PAM
- Rey-Campagnolle M
- Riccadonna X
- Richards R
- Richardson JD
- Richer JP
- Richeux JP
- Richter R
- Richter RH
- Richter-Was E
- Ridolfi G
- Rimoldi A
- Riu I
- Rizatdinova FK
- Roberts B
- Robertson L
- Robertson RJ
- Robertson S
- Robinson D
- Rodning N
- Roe S
- Rohlf J
- Rohrbach F
- Rolf A
- Romanov V
- Romanyuk A
- Ronceux B
- Rondot C
- Rosada A
- Rossel F
- Rossetto O
- Rossi L
- Rosso E
- Rothberg J
- Roy P
- Rozanov AN
- Rudolph G
- Rulikowska-Zarebska E
- Rumyantsev V
- Runge K
- Rusakovich N
- Rust DR
- Rutherfoord J
- Ryabchikov DI
- Ryabinin M
- Ryabov Yu
- Rzaev HJ
- Saadi Y
- Sadrozinski H
- Sala P
- Sala S
- Salihagic D
- Salomatin YuI
- Salt J
- Salukvadze R
- Sampsonidis D
- Sanchez F
- Sanchis E
- Sanchis MA
- Sanders H
- Santonico R
- Sasaki O
- Satsunkevich IS
- Saunders B J
- Sauvage D
- Sauvage G
- Savard P
- Savin A
- Savin I
- Savoy-Navarro A
- Sawyer L
- Saxon DH
- Says LP
- Schaapman J
- Schacht P
- Schael S
- Schafer F
- Schaffer AC
- Scherberger G
- Schioppa M
- Schmid B
- Schmid P
- Schmid T
- Schmitt M
- Schmucker H
- Schotanus DJ
- Schuh S
- Schuijlenburg H
- Schuller JP
- Schulman T
- Schulz T
- Schune P
- Schwemling P
- Schwindling J
- Scott IJ
- Sebastia A
- Sedda AF
- Sedykh Yu
- Segar AM
- Seguin-Moreau N
- Seiden A
- Seixas JM
- Selikov AV
- Sellden B
- Seller P
- Sellin PJ
- Seman M
- Semenov A
- Semenov PA
- Semenov S
- Senchishin V
- Sergeenko MN
- Serin L
- Sette G
- Sevior ME
- Shaevitz M
- Shah T P
- Shamov A
- Shank J
- Shankar K
- Shapiro M
- Shatalov P
- Shaver L
- Shchegelsky V
- Shchelchkov A
- Shein I
- Sherwood P
- Shikanian A
- Shmeleva A
- Shupe M
- Sicho P
- Siegrist J
- Simaitis VJ
- Simak V
- Simmons E
- Sinervo PK
- Sippach W
- Sirotenko VI
- Sisakian A
- Sitar B
- Sivoklokov SYu
- Skillicorn IO
- Skwarnicki T
- Slattery P
- Sliwa K
- Sloan T
- Smirnov AI
- Smirnov S
- Smirnova LN
- Smith G
- Smith KM
- Smith NA
- Smizanska M
- Smol A
- Snesarev A
- Snoeys W
- Snow S
- Snyatkov V
- Sobala A
- Sobie R
- Soderqvist J
- Soldatov AP
- Solodkov AA
- Solovianov OV
- Sondericker J
- Sopko B
- Sopromadze D
- Sosnovtsev V
- Sottini S
- Soukup J
- Spencer E
- Spieler H
- Spiridenkov E
- Spiwoks R
- Spooner NJC
- St. Denis R
- Stahl A
- Stairs GG
- Staley RJ
- Stanek R
- Stapnes S
- Starchenko EA
- Stastny J
- Staude A
- Stavrianakou M
- Stavropoulos G
- Stedron M
- Steinberg J
- Stephani D
- Stephens R
- Stoker D
- Strizenec P
- Strmen P
- Strong JA
- Stroynowski R
- Stumer I
- Suk M
- Sukhorukov AV
- Sulin V
- Surkov AN
- Susinno G
- Sutcliffe P
- Sytnik VV
- Szarka J
- Tafirout R
- Taguet JP
- Takai H
- Takashima R
- Takeda H
- Takeshita T
- Tanaka S
- Tappern GJ
- Tardell S
- Tartarelli F
- Tas P
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- Taylor GN
- Teiger J
- Telnov V
- Teplitz V
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- Teubert F
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- Thome ZD
- Thompson G
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- Thooris B
- Thulesius M
- Tikhomirov V
- Tikhonov VV
- Tikhonov Yu
- Tisserand V
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- Tittel K
- Tkachev L
- Toevs F
- Tokar S
- Tokmenin V
- Tolsma H
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- Topilin N
- Touchard F
- Tovey SN
- Tribanek W
- Trigari S
- Trigger I
- Trilling G
- Trischuk E
- Trka Z
- Tsesmelis E
- Tskhadadze E
- Tsukerman I
- Tsyupa Yu
- Tumakov VL
- Turcot A
- Turner PR
- Tvalashvili O
- Tyndel M
- Tzamarias SE
- Tzanakos GS
- Uberschar S
- Unal G
- Underwood DG
- Unel G
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- Usubov ZU
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- van den Berg FD
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- van Eijk B
- Vanickova M
- Vanyashin A
- Varner G
- Vartapetian A
- Varvell KE
- Vasey F
- Vasileva L
- Vazeille F
- Vedrine P
- Veillet JJ
- Velasco J
- Velikzhanin Yu
- Veneziano S
- Ventura G
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- Vermeulen JC
- Vernay E
- Vertogradov L
- Verzocchi M
- Veyssiere C
- Vichou I
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- Vinattieri A
- Virchaux M
- Visser EJ
- Vorobev AP
- Vorozhtsov S
- Voss R
- Vovenko AS
- Vrba V
- Vuillemin V
- Wadsworth BF
- Wagner D
- Wagner RG
- Wahlen H
- Walter C
- Walters CR
- Wang CH
- Wasserbaech S
- Wastie R L
- Watase Y
- Watkins PM
- Watson AT
- Webel M
- Weber C
- Weerts H
- Weidberg AR
- Weilhammer P
- Wellenstein H
- Wendler H
- Wermes N
- Werneke P
- Wesson D
- Whitaker JS
- White A
- White DJ
- White J
- Wickens FJ
- Wicklund A B
- Wijnen TAM
- Wilhelm I
- Wilhelm R
- Williams HH
- Williams ML
- Willis SE
- Willis W
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- Wingerter-Seez I
- Witzeling W
- Wolters H
- Wormald M
- Worstell W
- Wotschack J
- Wright M
- Wu SL
- Wu X
- Wunsch M
- Wunstorf R
- Wyllie KH
- Yakimenko M
- Yamagata T
- Yamamoto A
- Yamartino JM
- Yamdagni N
- Yarygin G
- Yasu Y
- Yoshida H
- Yu B
- Zacek V
- Zaets VG
- Zaitsev AM
- Zaitsev V
- Zanello L
- Zeldovich S
- Zhao T
- Zhao X
- Zhautykov B
- Zhemanov V
- Zhou B
- Zhukov VYu
- Zielinski M
- Zitoun R
- Zmushko V
- Zobernig G
- Zolnierowski Y
- Zoz R
- Zverev EG
- Zyazyulya FE
- Publication venue
- Publication date
- 01/01/1994
- Field of study