Detection of embryo mortality and hatch using thermal differences among incubated chicken eggs

Accurate diagnosis of both the stage of embryonic mortality and the hatch process in incubated eggs is a fundamental component in troubleshooting and hatchery management. However, traditional methods disturb incubation, destroy egg samples, risk contamination, are time and labour-intensive and require specialist knowledge and training. Therefore, a new method to accurately detect embryonic mortality and hatching time would be of significant interest for the poultry industry if it could be done quickly, cheaply and be fully integrated into the process. In this study we have continuously measured individual eggshell temperatures and the corresponding micro-environmental air temperatures throughout the 21 days of incubation using standard low-cost temperature sensors. Moreover, we have quantified the thermal interaction between eggs and air by calculating thermal profile changes (temperature drop time, drop length and drop magnitude) that allowed us to detect four categories of egg status (infertile/early death, middle death, late death and hatch) during incubation. A decision tree induction classification model accurately (93.3%) predicted the status of 105 sampled eggs in comparison to the classical hatch residue breakout analyses. With this study we have provided a major contribution to the optimisation of incubation processes by introducing an alternative method for the currently practiced hatch residue breakout analyses.status: publishe

Feulgen-DNA Content and C-Banding of Robertsonian Transformed Karyotypes in Dugesia Lugubris

SUMMARYIn the planarian species, Dugesia lugubris, two biotypes are found: E (2n = 8, n = 4) and F (2n = 6, n = 3); on the basis of karyometric studies it has been hypothesized that the second was derived from the first through a Robert-sonian mechanism of centric fusion. The quantitative cytochemical data reported here confirm the hypothesis of karyotype evolution, since there are no significant differences between the DNA content of the nucleus in the two biotypes. The regenerative blastemas of both biotypes contain a number of cellular populations with a variable Feulgen-DNA content; these correspond to successive doublings of the 2C diploid content. In addition, metaphase plates with multistranded chromosomes have been found. A difference between the chromosome C-banding in the two biotypes has also been observed

Higher levels of CO2 during late incubation alter the hatch time of chicken embryos

status: publishe

EFSUMB Recommendations and Guidelines for Gastrointestinal Ultrasound - Part 1: Examination Techniques and Normal Findings (Long version).

Abstract ▼ In October 2014 the European Federation of Societies for Ultrasound in Medicine and Biology formed a Gastrointestinal Ultrasound (GIUS) task force group to promote the use of GIUS in a clinical setting. One of the main objectives of the task force group was to develop clinical recommendations and guidelines for the use of GIUS under the auspices of EFSUMB. The first part, gives an overview of the examination techniques for GIUS recommended by experts in the field. It also presents the current evidence for the interpretation of normal sonoanatomical and physiological features as examined with different ultrasound modalities

EFSUMB Recommendations and Guidelines for Gastrointestinal Ultrasound - Part 1: Examination Techniques and Normal Findings (Short version)

Abstract ▼ In October 2014 the European Federation of Societies for Ultrasound in Medicine and Biology formed a Gastrointestinal Ultrasound (GIUS) task force group to promote the use of GIUS in a clinical setting. One of the main objectives of the task force group was to develop clinical recommendations and guidelines for the use of GIUS under the auspices of EFSUMB. The first part, gives an overview of the examination techniques for GIUS recommended by experts in the field. It also presents the current evidence for the interpretation of normal sonoanatomical and physiological features as examined with different ultrasound modalities

Search for weakly interacting sub-eV particles with the OSQAR laser-based experiment: results and perspectives

Recent theoretical and experimental studies highlight the possibility of new fundamental particle physics beyond the Standard Model that can be probed by sub-eV energy experiments. The OSQAR photon regeneration experiment looks for "Light Shining through a Wall" (LSW) from the quantum oscillation of optical photons into "Weakly Interacting Sub-eV Particles" (WISPs), like axion or axion-like particles (ALPs), in a 9 T transverse magnetic field over the unprecedented length of $2 \times 14.3$ m. No excess of events has been detected over the background. The di-photon couplings of possible new light scalar and pseudo-scalar particles can be constrained in the massless limit to be less than $8.0\times10^{-8}$ GeV$^{-1}$. These results are very close to the most stringent laboratory constraints obtained for the coupling of ALPs to two photons. Plans for further improving the sensitivity of the OSQAR experiment are presented.Comment: 7 pages, 7 figure