Maximum likelihood tree of the VP2 gene and partial NS1 gene of SePV.

<p>Phylogenetic maximum-likelihood tree with 500 bootstrap replicates of the nucleotide sequence of the VP2 genes (<b>A</b>) and partial NS genes (<b>B</b>) of SePV variants and various viruses of the genus <i>Erythroparvovirus</i>. Only bootstrap values >70 are indicated. Genbank accession numbers: SePV-HG06130: KM252691, SePV-HG020628.02: KM252694, SePV-PV121216.01: KM252698, SePV-PV880823.6: KM252692, SePV-PV880927.20: KM252693, SePV12410: KF373759, SePV-PV020628.13: KM252695, SePV-PV020718.12: KM252696, SePV-PV020719.08: KM252697, bovine parvovirus 3: AF406967, chipmunk parvovirus: GQ200736, rhesus macaque parvovirus: AF221122, simian parvovirus: U26342, pig-tailed macaque parvovirus: AF221123, human parvovirus B19-Au: M13178, human parvovirus B19-LaLi: AY044266, human parvovirus B19-V9: AJ249437.</p

Overview of characteristics of seals with SePV.

1<p>:UK: United Kingdom, NL: the Netherlands.</p>2<p>: based on results described previously <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0112129#pone.0112129-Rijks1" target="_blank">[14]</a>.</p>3<p>: NI: no information available.</p>4<p>: No PDV was detected <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0112129#pone.0112129-Rijks1" target="_blank">[14]</a>, although observed lesions were highly suggestive of infection with PDV.</p>5<p>: samples from a previous study <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0112129#pone.0112129-Bodewes1" target="_blank">[12]</a>.</p><p>Overview of characteristics of seals with SePV.</p

Geometric mean antibody titers and duration of antibodies against influenza A(H10N7).

<p>GMT (± s.d.) of seals with antibodies against influenza A(H10N7) virus in 2015 (<b>A</b>). The numbers next to the group names indicate the number of sera tested. Temporal change in HI antibody titers in four individual harbor seal weaners that tested seropositive upon admission (<b>B</b>).</p

Proportion of live-captured seals with antibodies against influenza A virus.

<p>Serum samples collected from live-captured (sub)adult harbor (<b>A</b>) and gray (<b>B</b>) seals were tested for the presence of antibodies against the NP using an influenza A virus NP blocking ELISA (white bars) and the hemagglutination inhibition test (gray bars). The numbers above the bars indicate number of sera tested.</p

Geographical distribution of seals with H10N7 antibodies in 2015 in the Netherlands.

<p>Map of the Netherlands with proportion (black sector) of the total (complete pie) harbor seal pups (A), harbor seal weaners (B), (sub)adult harbor seals (C) and non-pup gray seals (D) with antibodies against the seal HA of influenza A(H10N7) virus. The coastal regions where samples were collected in 2015 are indicated in dark grey: (Wadden Islands, W; Groningen, G; Friesland, F; North-Holland, N; South-Holland, S; and Zeeland, Z). The number above the pie charts indicate the number of serum samples tested per region. The map of the Netherlands was adapted from: <a href="https://pixabay.com/en/netherlands-holland-map-europe-303419/" target="_blank">https://pixabay.com/en/netherlands-holland-map-europe-303419/</a>.</p

Pairwise amino acid identity in the P1, P2, and P3 regions between ferret kobuvirus 38 (MpKoV38; KF006985) and bovine kobuvirus (BKoV; AB084788), human aichivirus (AIV; FJ890523), porcine kobuvirus (PKoV; GU292559), and klassevirus (Klasse; GQ184145).

<p>Pairwise amino acid identity in the P1, P2, and P3 regions between ferret kobuvirus 38 (MpKoV38; KF006985) and bovine kobuvirus (BKoV; AB084788), human aichivirus (AIV; FJ890523), porcine kobuvirus (PKoV; GU292559), and klassevirus (Klasse; GQ184145).</p

Variation in SePV sequences.

<p>Schematic overview of the partial NS gene and the complete VP2 gene that was amplified of 9 SePV variants. The consensus sequence of each virus was compared to the oldest sample available (HSePV-PV880823.6). Locations with nucleotide mutations are indicated in grey. Only in sample HSePV-121216.01, a nucleotide mutation resulted in an amino acid substitution (S540T).</p

Pairwise amino acid identity in the polyprotein P1, P2, and P3 regions between different parechovirus species.

<p>HPeV1 (JX575746), HPeV2 (AF055846), Ljungan (NC_003976), MpPeV1 (KF006989).</p

Phylogenetic analysis of ferret anellovirus.

<p>A phylogenetic tree of the partial ORF1 nucleotide sequence of ferret torque teno virus (MpfTTV1) and the corresponding region of representative human and animal anelloviruses was generated using MEGA5, with the neighbor-joining method with p-distance and 1,000 bootstrap replicates. Significant bootstrap values are shown. Genbank accession numbers are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071595#pone.0071595.s001" target="_blank">Table S1</a>.</p

Summary of mammalian viruses found in ferret fecal material.

a<p>Total no. of trimmed reads that were analyzed.</p>b<p>These animals had diarrhea.</p>c<p>NL, Netherlands; SE, Sweden.</p>d<p>FRCoV, ferret coronavirus; MpPV1, ferret papillomavirus; MpKoV, ferret kobuvirus; FRHEV, ferret hepevirus; MpPeV, ferret parechovirus; MpfTTV, ferret anellovirus.</p