Syndemics and the PrEP Cascade: Results from a Sample of Young Latino Men Who Have Sex with Men.

Young Latino men who have sex with men (MSM) are a highly vulnerable population for HIV infection. Pre-exposure prophylaxis (PrEP) is a novel biomedical HIV prevention tool that may aid in reducing the disparity in HIV incidence among Latino MSM. However, PrEP use is disproportionally low among Latino MSM and, therefore, identifying barriers along the PrEP continuum of care (the "PrEP cascade") would provide insight into how to best deploy PrEP interventions. Syndemics theory is a prominent framework employed in HIV prevention; however, to date, no known studies have applied this theory to PrEP. Thus, the aim of the current study was to explore the association between syndemics and the PrEP cascade, including the degree to which psychosocial and structural syndemic constructs are related to the PrEP cascade. Participants were 151 young Latino MSM (M age = 24 years; SD = 3) residing in San Diego, California, who completed a battery of online self-report measures. Results indicated high levels of syndemic indicators and varying levels of engagement across the PrEP cascade. As syndemic indicators increased, the odds of engagement across the PrEP cascade were significantly lowered. Psychosocial and structural syndemic factors accounted for unique variance in the PrEP cascade. Results highlight the need for combination interventions that address both psychosocial and structural barriers to PrEP use and persistence among young Latino MSM

An early warning for better planning of agricultural expansion and biodiversity conservation in the Orinoco high plains of Colombia

The eastern high plains of the Orinoco region in Colombia are known as ‘Altillanura’. They are considered the future agricultural frontier of Colombia. Unfortunately, an agricultural expansion without taking in consideration areas of high biodiversity and conservation of fragile ecosystems that are ecologically irreplaceable will likely fail in providing a sustainable grow. An orderly management planning of the territory based on scientific evidence is currently lacking for this region. Specifically, studies that combine biodiversity data and agricultural information are a major research gap. This study analyses the spatial patterns of species richness and endemism of flora and fauna at the site and subregional levels. We compared the spatial changes of biodiversity patterns with aspects related to the expansion of the agricultural frontier and its possible impacts. We found a west-to-east pattern; sites closer to the Andes were more exposed to ecological degradation than those in the east. Santa Rosalía and Puerto Lopez are the municipalities with the most remarkable species diversity. Conversely, La Primavera municipality has the most significant number of endemic species. Our spatial changes results raise the alarm showing that hotspots of diversity closer to the municipalities of Puerto Lopez and Puerto Gaitan are under more pressure than underdeveloped municipalities (La Primavera and Puerto Carreño). Our results could serve as a baseline to identify spatial changes of agrobiodiversity and a guideline for land-use planning, regional policies and local decision-makers to improve regional development in Colombia’s eastern plains region

In Vitro Fermentative Capacity of Equine Fecal Inocula of 9 fibrous Forages in the Presence of Different Doses of Saccharomyces cerevisiae

There is increasing interest in feeding fiber-based feeds as an alternative to high-starch cereal grains to horses as a means of meeting the energy demands and reduce various pathologies, such as gastric ulceration, hind-gut acidosis, laminitis, and colic [2,3], associated with feeding high levels of cereal grains. Depending on the forage type and time of harvest, forages of moderate to high nutritive value may meet the energy and crude protein (CP) requirements of horses [4]. Horses naturally use forage as a primary component of their diet, and forage is a basic necessity for normal function of the equine digestive system. High forage rations are desirable because they contain low levels of starch and sugar. Feeding a minimum 1% of body weight as fiber is very important to minimize the incidence of hindgut acidosis [5], colic [6], gastric ulcers [7], and stereotypical behaviors [8]. In tropical areas such as Mexico, forages used as feeds are generally low in digestibility and low in true protein [9]. Therefore, there is a need to develop feeding strategies which meet the requirements of performance horses while maintaining gut health and integrity.This experimentwas conducted to evaluate in vitro effects of equine fecal inocula fermentative capacity on 9 fibrous forages in the presence of Saccharomyces cerevisiae. The fibrous feeds were corn stover (Zeamays), oat straw(Avena sativa), sugarcane bagasseandleaves (Saccharum officinarum), llanero grass leaves (Andropogon gayanus), Taiwan grass leaves (Pennisetum purpureum), sorghum straw (Sorghum vulgare), and steria grass leaves (Cynodon plectostachyus). Fibrous feed samples were incubated with several doses of S. cerevisiae; 0 (control), 1.25 (low), 2.5 (medium) and 5 (high) mg/g dry matter (DM) of a commercial yeast product containing 1 1010/g. Fecal inoculumwas collected from4 adult horseswere fed onanamount of commercial concentrate and oat hay ad libitum. Gas production (GP)was recorded at 2, 4, 6, 8, 10, 12, 24, and 48 hours post inoculation. An interaction occurred between feeds and yeast dose for fecalpH(P<.01), asymptoticGP (b, ml/gDM); rate ofGP (c, /hr); initial delay beforeGP began (L, hours),GPat 4 hours and48 hours (P<.01), andGPat 8 hours (P<.01) andat 24 hours (P<.01). Differences in fecal fermentation capacity between the tropical and template grass (P < .05) occurred for fecal pH, c, and GP during first 12 hours, whereas differences occurred (P< .05) between the agriculture byproducts and the grasses for fecal pH, b, and GP from 8 to 48 hours. Fermentation capacity between straws versus not straws (P <.05) differed for fecal pH, b, and GP after 12 hours between straws versus not straws. Addition of S. cerevisiae to Z. mays stover reduced (P <.01) fecal pH and the c fraction with a higher (P <.01) b fraction versus the other feeds. From4 to 24 hours, S. officinarumbagasse improvedGP tothe highest values versus S. officinarum leaves. After 24 hours, Z. mays stover had the highest GP, whereas C. plectostachyus leaves had the lowest. There were no differences among the yeast doses for all measured parameters with the exception of L values (linear effect; P <.01). The Z. mays stover hadthe highestnutritive comparedtothe otherfibrous feeds.However, additionof S. cerevisiae at 2.5 to 5.0 g/kg DM improved fecal fermentation capacity of low-quality forage

A randomized phase II clinical trial of dendritic cell vaccination following complete resection of colon cancer liver metastasis

Surgically resectable synchronic and metachronic liver metastases of colon cancer have high risk of relapse in spite of standard-of-care neoadjuvant and adjuvant chemotherapy regimens. Dendritic cell vaccines loaded with autologous tumor lysates were tested for their potential to avoid or delay disease relapses (NCT01348256). Patients with surgically amenable liver metastasis of colon adenocarcinoma (n = 19) were included and underwent neoadjuvant chemotherapy, surgery and adjuvant chemotherapy. Fifteen patients with disease-free resection margins were randomized 1:1 to receive two courses of four daily doses of dendritic cell intradermal vaccinations versus observation. The trial had been originally designed to include 56 patients but was curtailed due to budgetary restrictions. Follow-up of the patients indicates a clear tendency to fewer and later relapses in the vaccine arm (median disease free survival –DFS-) 25.26 months, 95% CI 8. 74-n.r) versus observation arm (median DFS 9.53 months, 95% CI 5.32–18.88)

Pasado, presente y futuro de la vacunación anti-idiotipo

Cancer vaccines are conceived as therapeutic tools, in contrast to the prophylactic vaccines used to fight against infectious diseases. Among the most potent therapeutic vaccines, anti-idiotype vaccination is directed against the tumor idiotype, the only well-characterized tumor antigen displayed in neoplastic B-cells. Anti-idiotype vaccines have demonstrated clinical benefit against follicular lymphoma and are currently being evaluated in two different phase III clinical trials. Additional emerging strategies, which include the use of dendritic cells and the production of vaccines via molecular means will surely allow us to draw important conclusions concerning the treatment of cancer patients

CIGB-300, a synthetic peptide-based drug that targets the CK2 phosphoaceptor domain. Translational and clinical research

CK2 represents an oncology target scientifically validated. However, clinical research with inhibitors of the CK2-mediated phosphorylation event is still insufficient to recognize it as a clinically validated target. CIGB-300, an investigational peptide-based drug that targets the phosphoaceptor site, binds to a CK2 substrate array in vitro but mainly to B23/nucleophosmin in vivo. The CIGB-300 proapoptotic effect is preceded by its nucleolar localization, inhibition of the CK2-mediated phosphorylation on B23/nucleophosmin and nucleolar disassembly. Importantly, CIGB-300 shifted a protein array linked to apoptosis, ribosome biogenesis, cell proliferation, glycolisis, and cell motility in proteomic studies which helped to understand its mechanism of action. In the clinical ground, CIGB-300 has proved to be safe and well tolerated in a First-in-Human trial in women with cervical malignancies who also experienced signs of clinical benefit. In a second Phase 1 clinical trial in women with cervical cancer stage IB2/II, the MTD and DLT have been also identified in the clinical setting. Interestingly, in cervical tumors the B23/nucleophosmin protein levels were significantly reduced after CIGB-300 treatment at the nucleus compartment. In addition, expanded use of CIGB-300 in case studies has evidenced antitumor activity when administered as compassional option. Collectively, our data outline important clues on translational and clinical research from this novel peptide-based drug reinforcing its perspectives to treat cancer and paving the way to validate CK2 as a promising target in oncology.Fil: Perea, Silvio E.. Center for Genetic Engineering and Biotechnology; CubaFil: Baladron, Idania. Center for Genetic Engineering and Biotechnology; CubaFil: Garcia, Yanelda. Center for Genetic Engineering and Biotechnology; CubaFil: Perera, Yasser. Center for Genetic Engineering and Biotechnology; CubaFil: Lopez, Adlin. Center for Genetic Engineering and Biotechnology; CubaFil: Soriano, Jorge L.. Center for Genetic Engineering and Biotechnology; Cuba. General Hospital ‘‘Hermanos Ameijeiras’; CubaFil: Batista, Noyde. Center for Genetic Engineering and Biotechnology; Cuba. General Hospital ‘‘Hermanos Ameijeiras’; CubaFil: Palau, Aley. Center for Genetic Engineering and Biotechnology; Cuba. General Hospital ‘‘Hermanos Ameijeiras’; CubaFil: Hernández, Ignacio. Center for Genetic Engineering and Biotechnology; CubaFil: Farina, Hernán Gabriel. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Garcia, Idrian. Center for Genetic Engineering and Biotechnology; CubaFil: Gonzalez, Lidia. Center for Genetic Engineering and Biotechnology; CubaFil: Gil, Jeovanis. Center for Genetic Engineering and Biotechnology; CubaFil: Rodriguez, Arielis. Center for Genetic Engineering and Biotechnology; CubaFil: Solares, Margarita. Center for Genetic Engineering and Biotechnology; CubaFil: Santana, Agueda. Center for Genetic Engineering and Biotechnology; CubaFil: Cruz, Marisol. Center for Genetic Engineering and Biotechnology; CubaFil: Lopez, Matilde. Center for Genetic Engineering and Biotechnology; CubaFil: Valenzuela, Carmen. Center for Genetic Engineering and Biotechnology; CubaFil: Reyes, Osvaldo. Center for Genetic Engineering and Biotechnology; CubaFil: López Saura, Pedro A.. Center for Genetic Engineering and Biotechnology; CubaFil: González, Carlos A.. Center for Genetic Engineering and Biotechnology; CubaFil: Diaz, Alina. Center for Genetic Engineering and Biotechnology; CubaFil: Castellanos, Lila. Center for Genetic Engineering and Biotechnology; CubaFil: Sanchez, Aniel. Center for Genetic Engineering and Biotechnology; CubaFil: Betancourt, Lazaro. Center for Genetic Engineering and Biotechnology; CubaFil: Besada, Vladimir. Center for Genetic Engineering and Biotechnology; CubaFil: González, Luis J.. Center for Genetic Engineering and Biotechnology; CubaFil: Garay, Hilda. Center for Genetic Engineering and Biotechnology; CubaFil: Gómez, Roberto. Center for Genetic Engineering and Biotechnology; CubaFil: Gomez, Daniel Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; ArgentinaFil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Perrin, Phillipe. No especifíca;Fil: Renualt, Jean Yves. No especifíca;Fil: Sigman, Hugo. No especifíca;Fil: Herrera, Luis. Center for Genetic Engineering and Biotechnology; CubaFil: Acevedo, Boris. Center for Genetic Engineering and Biotechnology; Cub

Measurement of the cosmic ray spectrum above 4×10184{\times}10^{18} eV using inclined events detected with the Pierre Auger Observatory

A measurement of the cosmic-ray spectrum for energies exceeding $4{\times}10^{18}$ eV is presented, which is based on the analysis of showers with zenith angles greater than $60^{\circ}$ detected with the Pierre Auger Observatory between 1 January 2004 and 31 December 2013. The measured spectrum confirms a flux suppression at the highest energies. Above $5.3{\times}10^{18}$ eV, the "ankle", the flux can be described by a power law $E^{-\gamma}$ with index $\gamma=2.70 \pm 0.02 \,\text{(stat)} \pm 0.1\,\text{(sys)}$ followed by a smooth suppression region. For the energy ($E_\text{s}$) at which the spectral flux has fallen to one-half of its extrapolated value in the absence of suppression, we find $E_\text{s}=(5.12\pm0.25\,\text{(stat)}^{+1.0}_{-1.2}\,\text{(sys)}){\times}10^{19}$ eV.Comment: Replaced with published version. Added journal reference and DO

Energy Estimation of Cosmic Rays with the Engineering Radio Array of the Pierre Auger Observatory

The Auger Engineering Radio Array (AERA) is part of the Pierre Auger Observatory and is used to detect the radio emission of cosmic-ray air showers. These observations are compared to the data of the surface detector stations of the Observatory, which provide well-calibrated information on the cosmic-ray energies and arrival directions. The response of the radio stations in the 30 to 80 MHz regime has been thoroughly calibrated to enable the reconstruction of the incoming electric field. For the latter, the energy deposit per area is determined from the radio pulses at each observer position and is interpolated using a two-dimensional function that takes into account signal asymmetries due to interference between the geomagnetic and charge-excess emission components. The spatial integral over the signal distribution gives a direct measurement of the energy transferred from the primary cosmic ray into radio emission in the AERA frequency range. We measure 15.8 MeV of radiation energy for a 1 EeV air shower arriving perpendicularly to the geomagnetic field. This radiation energy -- corrected for geometrical effects -- is used as a cosmic-ray energy estimator. Performing an absolute energy calibration against the surface-detector information, we observe that this radio-energy estimator scales quadratically with the cosmic-ray energy as expected for coherent emission. We find an energy resolution of the radio reconstruction of 22% for the data set and 17% for a high-quality subset containing only events with at least five radio stations with signal.Comment: Replaced with published version. Added journal reference and DO