Chromosomal, epigenetic and microRNA-mediated inactivation of LRP1B, a modulator of the extracellular environment of thyroid cancer cells

The low-density lipoprotein receptor-related protein (LRP1B), encoding an endocytic LDL-family receptor, is among the 10 most significantly deleted genes across 3312 human cancer specimens. However, currently the apparently crucial role of this lipoprotein receptor in carcinogenesis is not clear. Here we show that LRP1B inactivation (by chromosomal, epigenetic and microRNA (miR)-mediated mechanisms) results in changes to the tumor environment that confer cancer cells an increased growth and invasive capacity. LRP1B displays frequent DNA copy number loss and CpG island methylation, resulting in mRNA underexpression. By using CpG island reporters methylated in vitro, we found that DNA methylation disrupts a functional binding site for the histone-acetyltransferase p300 located at intron 1. We identified and validated an miR targeting LRP1B (miR-548a-5p), which is overexpressed in cancer cell lines as a result of 8q22 DNA gains. Restoration of LRP1B impaired in vitro and in vivo tumor growth, inhibited cell invasion and led to a reduction of matrix metalloproteinase 2 in the extracellular medium. We emphasized the role of an endocytic receptor acting as a tumor suppressor by modulating the extracellular environment composition in a way that constrains the invasive behavior of the cancer cells

Distribuição do consumo de eletricidade na indústria no território em Portugal Continental e a potencial satisfação desta procura por fonte solar fotovoltaica

RESUMO: Este documento descreve o trabalho desenvolvido pelo LNEG com vista ao mapeamento do consumo de eletricidade na indústria em Portugal Continental e a potencial satisfação desta procura por energia solar fotovoltaica. O presente estudo derivou do esforço anterior para a identificação no país de áreas com menor sensibilidade (ambiental e patrimonial) para a instalação de centrais de geração de eletricidade de fonte renovável e ampliou o seu âmbito para a investigação da potencial satisfação do consumo industrial de eletricidade em áreas artificializadas para fins industriais e nas áreas envolventes destas consideradas menos sensíveis. A implementação de tecnologias de conversão de energia de fonte renovável de forma distribuída em ambiente construído/artificializado é fundamental para o país. No entanto, a análise pormenorizada desse universo necessita de mais tempo e recursos do que os disponíveis neste âmbito. Neste documento é apresentada uma análise exploratória da integração de sistemas solares fotovoltaicos na indústria, considerando o território por ela ocupado. Deve notar-se que os resultados apresentados traduzem a situação à data de janeiro / fevereiro de 2023, sendo que muita da informação utilizada tem um caráter dinâmico pelo que os resultados deste trabalho enquadram-se num contexto temporal definido, necessitando de atualizações periódicas.N/

Identificação de áreas com menor sensibilidade ambiental e patrimonial para localização de unidades de produção de eletricidade renovável

RESUMO: Este documento descreve o trabalho desenvolvido pelo LNEG com vista à futura identificação de “Go-To Areas” para a localização de unidades de produção de energia de fonte renovável em Portugal Continental. O trabalho decorreu no âmbito de um Grupo de Trabalho informal, coordenado pelo LNEG e envolvendo as seguintes entidades: a Agência Portuguesa do Ambiente (APA), a Direção Geral de Energia e Geologia (DGEG), a Direção Geral do Território (DGT), o Instituto da Conservação da Natureza e das Florestas (ICNF) e a Direção-Geral do Património Cultural (DGPC). Neste âmbito, pretendeu-se identificar no país as áreas com menor sensibilidade (ambiental e patrimonial) que possam vir a ser elegíveis para um processo de licenciamento mais simplificado para unidades de produção de energia de fonte renovável solar e eólica, permitindo deste modo acelerar a implementação sem comprometer outros valores ambientais e territoriais. As áreas resultantes serão áreas preferenciais do ponto de vista de simplificação do processo de licenciamento, mas não são exclusivas. Ou seja, as áreas aqui identificadas e as futuras “Go-To Areas” que possam vir a surgir não representam os únicos locais do país onde é possível implementar unidades renováveis. No resto do território a implementação é possível, de acordo com o normal processo de licenciamento. Este trabalho não delimita “Go-To Areas” renovável. Trata-se de um documento técnico que apresenta áreas sem condicionantes de exclusão que podem vir a ser consideradas para a definição formal de “Go-To Areas”. Deve notar-se que os resultados apresentados traduzem a situação à data de novembro / dezembro 2022, sendo que muita da informação utilizada tem um caráter dinâmico pelo que este trabalho deverá ser atualizado periodicamente.Este documento é complementado com vários ficheiros eletrónicos do Sistema de Informação Geográfica (SIG) contendo os dados de suporte produzidos/compilados. Por fim e não menos importante, este primeiro trabalho foca exclusivamente as áreas não artificializadas. Embora seja fundamental para o país a implementação de renováveis de forma distribuída em ambiente construído/artificializado, a análise desse universo necessita de mais tempo e recursos do que os disponíveis neste âmbito.SUMMARY: This document describes the work carried out by LNEG with a view to the future identification of “Go-To Areas” for the deployment of renewable energy power plants in mainland Portugal. The work took place within the scope of an informal Working Group, coordinated by LNEG and involving the following entities: the Portuguese Environment Agency (APA), the General Directorate of Energy and Geology (DGEG), the General Directorate of the Territory (DGT), the Institute for Nature Conservation and Forests (ICNF) and the Directorate-General for Cultural Heritage (DGPC). The aim was to identify the areas in the country with less sensitivity (regarding environment and heritage aspects) that could be eligible for a more simplified permitting process for solar and wind power plants, thus allowing to accelerate their implementation without compromising other environmental and territorial values. The resulting areas will potentially be candidates for power plants simplified permitting processes, although they are not exclusive. That is, the areas identified here and the future “Go-To Areas” that may arise are not the only places in the country where it is possible to implement renewable power plants. In the rest of the territory deployment is possible, following the normal permitting process. This work does not delimit renewable “Go-To Areas”. This is a technical document that presents areas without exclusion conditions that may be considered for the formal definition of future “Go-To Areas”. It should be noted that the results here presented reflect the situation as of November / December 2022. Since much of the information used is dynamic in nature this work should be periodically updated. This document is complemented with several electronic files from a Geographical Information System (GIS) containing the supporting data produced/compiled. Finally, this first work focuses exclusively on non-artificialized areas. Although it is essential for the country to implement distributed renewable power plants in the built/artificial environment, analyzing this universe requires more time and resources than are available in this stage.N/

Nuclear maturation kinetics of immature oocytes into preovulatory dominant follicle.

Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstract

Estimativa de potenciais técnicos de energia renovável em Portugal: eólico, solar fotovoltaico, solar concentrado, biomassa e oceanos

Executive Summary: There is a clear need to accelerate the energy transition, including the implementation of renewable electricity production plants, as well as the increase in consumption of other renewable energy carriers in buildings, industry, transport and other sectors. This work provides key information to make this transition possible, that is, the technical renewable energy potentials for Portugal. The aim is thus to contribute to policy support, as well as to decision-making by various Portuguese stakeholders (public and private) in the domains of energy, energy transition and greenhouse gases emissions mitigation. The work presents the technical renewable energy potentials for Portugal to: (i) decentralized solar photovoltaic (PV) plants in artificialized (or built-up) areas; (ii) centralized solar PV plants in non-artificialized (or natural) areas; (iii) concentrated solar power; (iv) onshore wind; (v) offshore wind (floating and fixed); (vi) bioenergy, and (vii) solar thermal. The wave energy primary energy resource potential is also presented (not the technical potential). The technical potential values of renewable energy sources (RES) presented are dynamic values, given the substantial uncertainty associated with their estimation. The study identifies technical RES potentials i.e., the technically viable energy generation achievable from a specific technology, considering the primary energy resource available and the geographic, environmental and land use limitations. RES economic potentials represent the fraction of RES technical potential that is economically viable, but they are not presented in this work. Likewise, this report does not address market potential, that translate the capacity and energy generation that the market effectively manages to implement. The presented RES technical potentials include the total capacity currently installed in the country. The technical potentials are estimated mostly for mainland Portugal, in most cases with a spatial disaggregation of at least NUT2 and sometimes for NUT5 and/or type of building. Despite adopting an approach based on a territorial analysis in which some areas of the country are excluded, this potential does not correspond to the work done in mapping less-sensitive areas towards future definition of RES “Go-To Areas”. The decentralized solar PV potential in artificialized areas is divided into 6 area types: industrial areas; commercial buildings; residential and mixed-use buildings; villas; health, education, cultural, tourist and military buildings, and other land uses (including parking lots and patios, ports, waste and wastewater treatment infrastructure, sports facilities, among others). It is estimated a technical potential of 23.33 GW that could generate up to 36.84 TWh/year. This potential is distributed throughout the entire territory of mainland Portugal but is higher in the North and Center regions. The RES technical potential for centralized solar PV was estimated as a range of values that translate the uncertainty associated with using different levels of concern in excluding certain areas in which solar PV can be deployed (for example to safeguard ecosystems, water resources, agriculture or archaeological heritage). The centralized solar PV potential varies between 168.82 GW and 45.63 GW. The maximum threshold of installed capacity could generate 278.11 TWh/year of electricity. The value is high and reflects on the one hand, the excellence of the solar resource throughout the country, and on the other, the large size of the considered areas. The CSP potential is 62.6 GW with a corresponding electrical production potential of 183.61 TWh/year. It is mainly located in the Alentejo region, although other areas have also been identified in other regions of the country. The wind onshore technical potential is 15.7 GW, that could generate 37.13 TWh/year, taking into account the safeguarding of various areas for the protection of ecosystems and also social acceptability issues. In the case of offshore wind and considering a capacity density of 4 MW/km2 for floating offshore and 5.5 MW/km2 for fixed offshore, a total of 36 GW and 2 GW are obtained, respectively. This capacity could generate up to 126.14 TWh/year (floating offshore) or 6.31 TWh/year (fixed offshore). The solar thermal energy potential focused residential and service buildings (such as nursing homes, barracks, etc., tourism, hospitals, indoor swimming pools and other sports facilities). The potential is of 0.95 GWt and 0.95 TWh/year for service buildings, 7.26 GWt and 5.84 TWh/year for residential buildings. For industry there is a potential of 1.06 GWt, which could generate up to 1.15 TWh/year for applications up to 160 ºC. The total technical potential of solar thermal is 9.25 GWt and 7.93 TWh/year of thermal energy generated, with a substantial weight of residential buildings in the total value. Potential values are disaggregated by NUTS III and type of building. In terms of biomass and bioenergy potential, annual values of forest biomass, agricultural biomass, agro-industrial waste, urban waste and wastewater treatment are estimated, totaling around 58 TWh/year. Regarding the production of biofuels (HVO and FAME) it is estimated that the annual production of domestic used oils and other similar residues is 1.4 TWh/year. The use of oils from food crops such as soybean, sunflower and rapeseed is limited by European (and national) policy guidelines and is 2.1 TWh/year. Regarding wave energy, the resource potential is estimated between 1.4 GW for 80 m bathymetry and 4.8 for 20 m bathymetry. There are substantial uncertainties associated with the presented values, inherent to the methodological approach considered. Nevertheless, these estimates are a valuable starting point to be refined and improved in subsequent updates.N/

CMS physics technical design report : Addendum on high density QCD with heavy ions

Peer reviewe

Why Are Outcomes Different for Registry Patients Enrolled Prospectively and Retrospectively? Insights from the Global Anticoagulant Registry in the FIELD-Atrial Fibrillation (GARFIELD-AF).

Background: Retrospective and prospective observational studies are designed to reflect real-world evidence on clinical practice, but can yield conflicting results. The GARFIELD-AF Registry includes both methods of enrolment and allows analysis of differences in patient characteristics and outcomes that may result. Methods and Results: Patients with atrial fibrillation (AF) and ≥1 risk factor for stroke at diagnosis of AF were recruited either retrospectively (n = 5069) or prospectively (n = 5501) from 19 countries and then followed prospectively. The retrospectively enrolled cohort comprised patients with established AF (for a least 6, and up to 24 months before enrolment), who were identified retrospectively (and baseline and partial follow-up data were collected from the emedical records) and then followed prospectively between 0-18 months (such that the total time of follow-up was 24 months; data collection Dec-2009 and Oct-2010). In the prospectively enrolled cohort, patients with newly diagnosed AF (≤6 weeks after diagnosis) were recruited between Mar-2010 and Oct-2011 and were followed for 24 months after enrolment. Differences between the cohorts were observed in clinical characteristics, including type of AF, stroke prevention strategies, and event rates. More patients in the retrospectively identified cohort received vitamin K antagonists (62.1% vs. 53.2%) and fewer received non-vitamin K oral anticoagulants (1.8% vs . 4.2%). All-cause mortality rates per 100 person-years during the prospective follow-up (starting the first study visit up to 1 year) were significantly lower in the retrospective than prospectively identified cohort (3.04 [95% CI 2.51 to 3.67] vs . 4.05 [95% CI 3.53 to 4.63]; p = 0.016). Conclusions: Interpretations of data from registries that aim to evaluate the characteristics and outcomes of patients with AF must take account of differences in registry design and the impact of recall bias and survivorship bias that is incurred with retrospective enrolment. Clinical Trial Registration: - URL: http://www.clinicaltrials.gov . Unique identifier for GARFIELD-AF (NCT01090362)