O amor, o sorriso e o povo brasileiro: Vinícius de Moraes e os movimentos culturais do Brasil contemporâneo

Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Filosofia e Ciências Humanas, Programa de Pós-Graduação em Sociologia Política, Florianópolis, 2006.Este estudo propõe uma relação entre produção cultural e contexto social através da análise das composições de Vinicius de Moraes, tendo com referência duas de suas principais obras: Chega de Saudade e Gente Humilde. Para a realização desta pesquisa, inseriu-se o poeta no contexto histórico da época em que as letras foram feitas, além de se verificar também o quadro político vivido por Moraes em toda sua trajetória (1913 até 1980). Em um breve histórico, foram organizados momentos políticos, fazendo-se um levantamento dos presidentes do Brasil e as características de seus governos. Em seguida, apresenta-se um relato a respeito da vida de Vinicius de Moraes, suas profissões, suas viagens, suas parcerias e seus ideais políticos. Abordou-se ainda o contexto musical no qual as duas letras foram transformadas em músicas, descrevendo a Bossa Nova e as Músicas de Protesto. Ao final, analise-se as letras de Chega de Saudade e Gente Humilde, do ponto de vista da relação do poeta com suas parcerias, com o público e consigo.This study it considers a relation between cultural production and social context through the analysis of the production of Vinicius de Moraes having with reference two of its main compositions: #Chega de Saudade# and #Gente Humilde#. To build this research it was necessary to insert the poet into the historical context of the time the letters had been made and also verify the political context that Moraes lived during his entire trajectory (1913 up to 1980). In a brief description, the political setting had been rebuilt, including Brazilian Presidents and their governments# characteristics. After that, it was made a narrative regarding Vinicius de Moraes life, his professions, his trips, his partnerships and his political ideals. The musical context in which the two lyrics were transformed into music was also considered, describing the #Bossa Nova# and the Music of Protest. At last it was carried out an analysis of the songs #Chega de Saudade#and #Gente Humilde# considering the relation of the poet with his partnerships, with the public and with himself

Buscando o corpo cênico : play-fight na vivência de um corpo poético e disponível

Trabalho de Conclusão de Curso (graduação)—Universidade de Brasília, Instituto de Artes, Departamento de Artes Cênicas, 2021.Este Trabalho de Conclusão de Curso do Bacharelado em Artes Cênicas tem como objetivo avaliar o benefício do Play-fight, uma prática-pesquisa desenvolvida por Bruno Caverna, para o acervo de artistas da cena interessadas e interessados em desenvolver a sensibilidade, expressividade, poeticidade e disponibilidade do corpo; um corpo fluido centrado no presente do presente. Para contemplar aspectos lúdicos dessa prática e relaciona-la à atividade teatral, me embaso principalmente na minha experiência com o objeto desse estudo e no conceito de jogos e brincadeiras utilizada pelo sociólogo Roger Caillois, em diálogo com alguns dos autores com quem tive contato na minha graduação.This Bachelor's Dissertation in Performing Arts aims to assess the benefit of Play-fight, a practice as research developed by Bruno Caverna, for the collection of stage artists interested in developing the sensitivity, expressiveness, poeticity and availability of the body; a fluid body centered on the present of the present. To contemplate the playful aspects of this practice and relate it to theatrical activity, I draw mainly on my experience with the object of this study and on the concept of play and games used by sociologist Roger Caillois, in dialogue with some of the authors I had contact with during my graduation

Gene-based Interventions for Cancer Immunotherapy

Immunotherapy of cancer has deservedly gained much attention in the past few years and is likely to continue to advance and become a fundamental cancer treatment. While vaccines, chimeric antigen receptor (CAR) T cells and checkpoint blockade have received the lion’s share of the attention, an important direct role for gene transfer as an immunotherapy is emerging. For example, oncolytic viruses induce immunogenic cell death, thus liberating both antigens and the signals that are necessary for the activation of antigen-presenting cells, ensuring stimulation of an adaptive response. In another example, transfer of prodrug converting enzymes, such as the herpes simplex virus-thymidine kinase (HSV-tk) gene or the cytosine deaminase gene, has been shown to promote an immune response, thus functioning as immunotherapies. Alternatively, our own work involves the use of nonreplicating viral vectors for the simultaneous delivery of gene combinations that promote both cell death and an immune response. In fact, our gene transfer approach has been applied as a vaccine, immunotherapy or in situ gene therapy, resulting in immunogenic cell death and the induction of a protective immune response. Here, we highlight the development of these approaches both in terms of technical advances and clinical experience

Aproveitamento de resíduos agrícolas no cultivo do cogumelo medicinal Ganoderma lucidum utilizando a tecnologia chinesa “JunCao”Use of agricultural residues in the cultivation of the medicinal mushroom Ganoderma lucidum using the “Jun-Cao” Chinese technology

Para o preparo de substratos de cultivo de cogumelos comestíveis, tem-se pesquisado processos biotecnológicos que utilizem resíduos lignocelulósicos que são descartados em grandes quantidades no meio ambiente. Assim, o objetivo do presente trabalho foi avaliar o aproveitamento de resíduos agrícolas para o cultivo do cogumelo medicinal Ganoderma lucidum e, também, quantifcar as massas dos basidiomas frescos e secos, o número de basidiomas produzidos por cada tratamento durante o ciclo de cultivo, a perda de matéria orgânica, efciência biológica e a relação C/N. Foi utilizada a linhagem GLM-10/02 de G. lucidum para oito tratamentos, cada qual com 10 repetições, totalizando 80 pacotes de 700g de substrato. Os tratamentos foram delineados como: 1- palha de cana de açúcar, 2- palha de milho, 3- casca de arroz, 4- capim coast-cross, 5- serragem de eucalipto, 6- serragem de eucalipto suplementada com palha de cana de açúcar, 7- serragem de eucalipto suplementada com casca de arroz e 8- serragem de eucalipto suplementada com capim coast-cross. Todos os tratamentos receberam 18% de farelo de trigo e 2% de calcário calcítico e tiveram a umidade ajustada para 60%. Os dados obtidos pela efciência biológica, a massa dos basidiomas frescos e secos e a relação C/N demonstram que todos os tratamentos foram satisfatórios, com exceção da casca de arroz, apresentando um grande potencial de conversão de biomassa fúngica.AbstractFor the preparation of substrates for the cultivation of edible mushrooms,biotechnological processes that use lignocellulosic residues discarded in large amounts in the environment have been searched. Tus, the objective of the present work was to evaluate the use of agricultural residues in the cultivation of the medicinal mushroom Ganoderma lucidum and also quantify the masses of fresh and dry basidiomata, loss of organic matter, biological efciency and C/N ratio. Te strain GLM-10/02 of G. lucidum was used for eight treatments, with 10 repetitions each, totalizing 80 packs of 700g of substrate. Te treatments were outlined as: 1- sugar cane straw, 2- corn straw, 3- rice husk, 4- coast-cross grass, 5- eucalyptus sawdust, 6- eucalyptus sawdust supplemented with sugar cane straw, 7- eucalyptus sawdust supplemented with rice husk, 8-eucalyptus sawdust supplemented with coast-cross grass. All treatments were added with 18% of wheat bran and 2% of limestone, with moisture adjusted to 60%. Te data obtained from biological efciency, mass of fresh and dry basidiomata and C/N ratio show that all treatments were satisfactory, showing great potential for conversion into fungal biomass

Reflexões sobre a terminalidade da vida na formação e no cotidiano do profissional medico / Reflections on terminality of live in the training and in the daily life of the medical professional

A morte e o morrer são temas pertinentes ao cotidiano de profissionais de saúde e ao processo de aprendizagem de acadêmicos de medicina. Nesse sentido, diante das poucas oportunidades de se questionar os sentimentos e a compreensão destes profissionais com relação ao morrer dos pacientes durante a graduação e durante o cotidiano profissional, o objetivo deste trabalho foi o de conhecer as percepções de médicos, incluindo docentes em curso de medicina, sobre situações que envolvam a terminalidade da vida. O presente estudo resulta de uma pesquisa qualitativa e descritiva do tipo corte transversal, com diretriz metodológica do discurso do sujeito coletivo, realizado por meio da aplicação de questionários em profissionais médicos atuantes nos Hospital das Clínicas Samuel Libânio da cidade de Pouso Alegre, Minas Gerais.

The Brazilian Registry of Adult Patient Undergoing Cardiovascular Surgery, the BYPASS Project: Results of the First 1,722 Patients

Objective: To report the early results of the BYPASS project - the Brazilian registrY of adult Patient undergoing cArdiovaScular Surgery - a national, observational, prospective, and longitudinal follow-up registry, aiming to chart a profile of patients undergoing cardiovascular surgery in Brazil, assessing the data harvested from the initial 1,722 patients. Methods: Data collection involved institutions throughout the whole country, comprising 17 centers in 4 regions: Southeast (8), Northeast (5), South (3), and Center-West (1). The study population consists of patients over 18 years of age, and the types of operations recorded were: coronary artery bypass graft (CABG), mitral valve, aortic valve (either conventional or transcatheter), surgical correction of atrial fibrillation, cardiac transplantation, mechanical circulatory support and congenital heart diseases in adults. Results: 83.1% of patients came from the public health system (SUS), 9.6% from the supplemental (private insurance) healthcare systemsand 7.3% from private (out-of-pocket) clinic. Male patients comprised 66%, 30% were diabetics, 46% had dyslipidemia, 28% previously sustained a myocardial infarction, and 9.4% underwent prior cardiovascular surgery. Patients underwent coronary artery bypass surgery were 54.1% and 31.5% to valve surgery, either isolated or combined. The overall postoperative mortality up to the 7th postoperative day was 4%for CABG was 2.6%, and for valve operations, 4.4%. Conclusion: This first report outlines the consecution of the Brazilian surgical cardiac database, intended to serve primarily as a tool for providing information for clinical improvement and patient safety and constitute a basis for production of research protocols.Univ Fed Sao Paulo UNIFESP EPM, Hosp Sao Paulo, Sao Paulo, SP, BrazilHosp Caridade Sao Vicente Paulo, Jundiai, SP, BrazilInst Med Integral Prof Fernando Figueira IMIP, Recife, PE, BrazilHosp Base FUNFARME & FAMERP, Sao Jose Do Rio Preto, SP, BrazilIMC, Sao Jose Do Rio Preto, SP, BrazilIrmandade Santa Casa Sao Paulo INCT HPV, Fac Ciencias Med Santa Casa Sao Paulo, Sao Paulo, SP, BrazilFundacao Univ Cardiol, Inst Cardiol Rio Grande do Sul, Porto Alegre, RS, BrazilInst Coracao Natal, Natal, RN, BrazilInst Cardiol Dist Fed, Brasilia, DF, BrazilUniv Fed Maranhao HU UFMA, Univ Hosp, Sao Luis, MA, BrazilHosp Evangelico, Cachoeiro De Itapemirim, ES, BrazilHosp Coracao Sergipe, Aracaju, SE, BrazilHosp Nossa Senhora Salete, Inst Cirurgia Cardiovasc ICCV, Cascavel, PR, BrazilHosp Wilson Rosado, Mossoro, RN, BrazilHosp Bosque Saude, Sao Paulo, SP, BrazilHosp Univ Santa Maria, Santa Maria, RS, BrazilHosp Coracao HCor, Sao Paulo, SP, BrazilHosp Coracao IP HCor, Ins Pesquisa, Sao Paulo, SP, BrazilInst Coracao InCor, Sao Paulo, SP, BrazilUniv Fed Sao Paulo UNIFESP EPM, Hosp Sao Paulo, Sao Paulo, SP, BrazilWeb of Scienc

Haplotypes of the bovine IgG2 heavy gamma chain in tick-resistant and tick-susceptible breeds of cattle

Bovines present contrasting, heritable phenotypes of infestations with the cattle tick, Rhipicephalus (Boophilus) microplus. Tick salivary glands produce IgG-binding proteins (IGBPs) as a mechanism for escaping from host antibodies that these ectoparasites ingest during blood meals. Allotypes that occur in the constant region of IgG may differ in their capacity to bind with tick IGBPs; this may be reflected by the distribution of distinct allotypes according to phenotypes of tick infestations. In order to test this hypothesis, we investigated the frequency of haplotypes of bovine IgG2 among tick-resistant and tick-susceptible breeds of bovines. Sequencing of the gene coding for the heavy chain of IgG2 from 114 tick-resistant (Bos taurus indicus, Nelore breed) and tick-susceptible (B. t. taurus, Holstein breed) bovines revealed SNPs that generated 13 different haplotypes, of which 11 were novel and 5 were exclusive of Holstein and 3 of Nelore breeds. Alignment and modeling of coded haplotypes for hinge regions of the bovine IgG2 showed that they differ in the distribution of polar and hydrophobic amino acids and in shape according to the distribution of these amino acids. We also found that there was an association between genotypes of the constant region of the IgG2 heavy chain with phenotypes of tick infestations. These findings open the possibility of investigating if certain IgG allotypes hinder the function of tick IGBPs. If so, they may be markers for breeding for resistance against tick infestations

Statement of Second Brazilian Congress of Mechanical Ventilarion : part I

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Rationale, study design, and analysis plan of the Alveolar Recruitment for ARDS Trial (ART): Study protocol for a randomized controlled trial

Background: Acute respiratory distress syndrome (ARDS) is associated with high in-hospital mortality. Alveolar recruitment followed by ventilation at optimal titrated PEEP may reduce ventilator-induced lung injury and improve oxygenation in patients with ARDS, but the effects on mortality and other clinical outcomes remain unknown. This article reports the rationale, study design, and analysis plan of the Alveolar Recruitment for ARDS Trial (ART). Methods/Design: ART is a pragmatic, multicenter, randomized (concealed), controlled trial, which aims to determine if maximum stepwise alveolar recruitment associated with PEEP titration is able to increase 28-day survival in patients with ARDS compared to conventional treatment (ARDSNet strategy). We will enroll adult patients with ARDS of less than 72 h duration. The intervention group will receive an alveolar recruitment maneuver, with stepwise increases of PEEP achieving 45 cmH(2)O and peak pressure of 60 cmH2O, followed by ventilation with optimal PEEP titrated according to the static compliance of the respiratory system. In the control group, mechanical ventilation will follow a conventional protocol (ARDSNet). In both groups, we will use controlled volume mode with low tidal volumes (4 to 6 mL/kg of predicted body weight) and targeting plateau pressure <= 30 cmH2O. The primary outcome is 28-day survival, and the secondary outcomes are: length of ICU stay; length of hospital stay; pneumothorax requiring chest tube during first 7 days; barotrauma during first 7 days; mechanical ventilation-free days from days 1 to 28; ICU, in-hospital, and 6-month survival. ART is an event-guided trial planned to last until 520 events (deaths within 28 days) are observed. These events allow detection of a hazard ratio of 0.75, with 90% power and two-tailed type I error of 5%. All analysis will follow the intention-to-treat principle. Discussion: If the ART strategy with maximum recruitment and PEEP titration improves 28-day survival, this will represent a notable advance to the care of ARDS patients. Conversely, if the ART strategy is similar or inferior to the current evidence-based strategy (ARDSNet), this should also change current practice as many institutions routinely employ recruitment maneuvers and set PEEP levels according to some titration method.Hospital do Coracao (HCor) as part of the Program 'Hospitais de Excelencia a Servico do SUS (PROADI-SUS)'Brazilian Ministry of Healt

Canine model of melanoma immunotherapy using adenoviral vectors carrying p14Arf and interferon-beta cDNAs

O melanoma é um tipo de câncer agressivo de difícil tratamento em casos avançados tanto na medicina humana quanto na veterinária. Apesar do progresso considerável nas propostas terapêuticas, as taxas de mortalidade ainda são elevadas, tornando necessário o desenvolvimento de novas abordagens terapêuticas. Os melanomas frequentemente apresentam disfunções na via de p53, embora frequentemente retenha a proteína p53 selvagem. Em estudos anteriores, nosso grupo utilizou vetores adenovirais para a transferência genica de p14ARF (proteína supressora de tumor) a fim de reativar a via p53 em células murinas e humanas, tanto in vitro quanto vivo. A co-transdução com IFN (citocina imunomoduladora) induziu níveis especialmente elevados de morte celular juntamente com a liberação de marcadores imunogênicos de morte celular in vitro, redução significativa da progressão tumoral e estimulação de fortes respostas imunes in vivo. Antes de prosseguirmos para testes em humanos, desejamos verificar estes resultados em modelo animal que represente melhor a complexidade dos casos humanos de melanoma, incluindo a capacidade de formar tumores espontâneos e metástases. Portanto, este trabalho teve como objetivo desenvolver vetores adenovirais que codificam os genes p14ARF e IFN caninos, a fim de validar nossa abordagem em um modelo utilizando linhagens celulares de melanoma oral canino previamente estabelecidas em nosso laboratório. Consistente com nossos estudos anteriores em linhagens celulares humanas e de camundongos, observamos que essa combinação de vetores induziu morte celular acompanhada pela liberação de fatores imunogênicos, como ATP e HMGB1 nas linhagens celulares caninas GAB-F6 e BAN-C10. Num modelo de xenoenxerto de terapia genética in situ, demonstramos a inibição da progressão tumoral in vivo, o atraso no desenvolvimento do tumor e a sobrevida prolongada dos animais. Estes resultados apoiam o futuro teste desta abordagem terapêutica em pacientes veterinários, um passo importante no desenvolvimento da nossa terapia genética para o melanomaMelanoma is an aggressive kind of cancer, difficult to treat in advanced cases in both human and veterinary medicine. Despite the considerable progress in therapeutic proposals, mortality rates are still high, making it necessary to develop new therapeutic approaches. Melanomas often present a dysfunctional p53 pathway, although it frequently retains the wild type protein. In previous studies, our group has used adenoviral vectors for the transfer of p14ARF (tumour suppressor protein) in order to reactivate the p53 pathway in murine and human cells, both in vitro and vivo. Co-transduction with IFN (immunomodulatory cytokine) induced especially high levels of cell killing along with the release of immunogenic cell death markers in vitro, significant reduction of tumour progression and stimulation of a strong immune responses in vivo. Before moving to human tests, we wish to verify these outcomes in animal models that more closely represent the complexity of human cases of melanoma, including the capability to form spontaneous tumours and metastases. Therefore, this work aimed to develop adenoviral vectors encoding the canine p14ARF and IFN genes in order to validate our approach in a model utilizing canine oral melanoma cell lines previously established in our laboratory. Consistent with our previous studies in mouse and human cell lines, we observed that this combination of vectors induced cell death accompanied by the release of immunogenic factors, such as ATP and HMGB1 in the canine cell lines GAB-F6 and BAN-C10. In a xenograft model of in situ gene therapy, we demonstrated the inhibition of tumour progression in vivo, and delay of tumour development and extended survival. These results support testing this therapeutic approach in veterinary patients, an important step in the development of our melanoma gene therap