The importance of pepsin‐like acid protease quantification in aquaculture studies: a revision of available procedures and presentation of a new protocol for its assessment

Assessing the activity of acid pepsin‐like protease is a common procedure in many biological, physiological and nutritional studies in gastric fish species. After reviewing the available literature on fish digestive acid proteases (stomach acid pepsin‐like proteases), especially those species of relevance for aquaculture purposes, published during a time span of ten years (2008–2018), authors detected some possible methodological and/or interpretative inconsistencies in this kind of studies when quantifying pepsin‐like enzymes. Thus, we concluded that special attention should be paid on the following issues when assessing digestive acid protease activity: (i) the proper preparation of stomach extracts, (ii) the use of the proper reaction conditions at the normal range of physiological pH and temperature values, and (iii) the proper quantification of the number of peptide bonds hydrolyzed for acid protease unit calculation. The implementation of these recommendations, and the proposal of more suitable (previously tested) methodological steps, will promote the standardization of acid pepsin‐like protease analytical procedures, as well as improve the reliability of comparative studies dealing with this proteolytic enzyme between different fish species of aquacultural importance.info:eu-repo/semantics/acceptedVersio

Propuesta de educación ambiental como innovación social

no aplicaEl alcance del estudio realizado consiste en sistematizar la experiencia “Educación Ambiental desde el modelo sistémico” realizado por Ruano y Tobar (2017), como tesis de Maestría en Educación de la Universidad del Valle, implementada en la Institución Educativa Antonio José de Sucre del municipio de Vijes, que cumple con los criterios para ser considerado una innovación social. Para elaborar este documento se utilizó como método la sistematización de experiencia, el cual está dividido en cuatro tiempos, que son: el punto de partida, las preguntas iniciales, recuperación del proceso vivido y la reflexión de fondo: ¿por qué pasó lo que pasó? Se llegó a identificar que es una innovación social- educativa; así mismo se encontró en el grado de maduración de la experiencia TRL 7 de la actividad de gestión del proceso.The scope of the study carried out consists of systematizing the experience "Environmental Education from the systemic model" carried out by Ruano and Tobar (2017), as a Master's thesis in Education from the Universidad del Valle, implemented in the Antonio José de Sucre Educational Institution of the municipality de Vijes, which meets the criteria to be considered a social innovation. To prepare this document, the systematization of experience was used as a method, which is divided into four stages, which are: the starting point, the initial questions, recovery of the process lived and the background reflection: why did what happened happen? It was identified that it is a social-educational innovation; Likewise, it was found in the degree of maturation of the TRL 7 experience of the process management activity

Relación masa-radio para estrellas que provienen de la evolución binaria con transferencia de masa

Uno de los principales vínculos que se pueden establecer entre los parámetros fundamentales en las estrellas es la relación que existe entre su masa y su radio. Desde un punto de vista teórico, esta relación se ha establecido para estrellas en distintos momentos de su evolución: en la secuencia principal, en estado de gigantes (entre la masa del núcleo y el radio), cuando alcanza el estado de enana blanca. Además, es en los sistemas binarios en los que estos parámetros pueden ser estimados de manera confiable. Por otro lado, es bien sabido que la mayoría de las estrellas se encuentran en sistemas binarios, siendo un caso particular los sistemas binarios con transferencia de masa a través del desborde del lóbulo de Roche (sistemas binarios interactuantes). En este trabajo, se estudia la relación masa-radio para estrellas provenientes de sistemas binarios interactuantes, utilizando simulaciones numéricas provenientes de nuestro código de evolución estelar en sistemas binarios con transferencia de masa. En particular, estamos interesados en extender la relación masa-radio al caso de enanas blancas de helio de muy baja masa, provenientes de la evolución estelar en estos escenarios. Compararemos, además, nuestros resultados con una muestra observacional adecuada, para ver cómo cumplen la relación masa-radio estrellas que evolucionan en estos escenarios. Obtenemos que se ajusta bien a lo propuesto y a la muestra observacional.Facultad de Ciencias Astronómicas y Geofísica

Simultaneous retrieval of optical and geometrical parameters of multilayered turbid media via state-estimation algorithms

In the present paper we propose an implementation of the Kalman filter algorithm, which allows simultaneous recovery of the absorption coefficient, the reduced scattering coefficient and the thicknesses of multi-layered turbid media, with the deepest layer taken as semi-infinite. The approach is validated by both Monte Carlo simulations and experiments, showing good results in structures made up of four layers. As it is a Bayesian algorithm, prior knowledge can be included to improve the accuracy of the retrieved unknowns. One of the most promising applications of this approach is the capability of real-time monitoring of living organs by near infrared spectroscopy. In particular, determination of blood perfusion in the adult head is one of the desired goals, allowing continuous control of stroke patients. This demands accurate measurement of the optical properties, especially absorption, of the head layers, from scalp to the cortex.Fil: García, Héctor Alfredo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires; ArgentinaFil: Baez, Guido Rodrigo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires; ArgentinaFil: Pomarico, Juan Antonio. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires; Argentin

Key market values for bottled wine in an emerging market: product attributes or business strategy?

This article focuses on the emerging bottled Chilean red wine market and studies the main determinants of the consumer price of wine sold on the domestic market. A hedonic price function was estimated for a sample of 810 wines using a quantile regression (QR) model. The database contains three variables groups to explain price: objective variables (national, international, and vine quality designations), subjective variables (wine score), and business strategies used by wine producers.Postprint (author's final draft

Implementation of the extended Kalman filter for determining the optical and geometrical properties of turbid layered media by time-resolved single distance measurements

In this article we propose an implementation of the extended Kalman filter (EKF) for the retrieval of optical and geometrical properties in two-layered turbid media assuming a dynamic setting, where absorption of each layer was changed in different steps. Prior works implemented the EKF in frequency-domain with several pairs of light sources and detectors and for static parameters estimation problems. Here we explore the use of the EKF in single distance, time-domain measurements, together with a corresponding forward model. Results show good agreement between retrieved and nominal values, with rather narrow analytical credibility intervals, indicating that the recovery process has low uncertainty, especially for the absorption coefficients.Fil: Baez, Guido Rodrigo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires; ArgentinaFil: García, Héctor Alfredo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires; ArgentinaFil: Grosenick, Dirk. Physikalisch-technische Bundesanstalt; AlemaniaFil: Wabnitz, Heidrun. Physikalisch-technische Bundesanstalt; Alemani

Formalization of an enterprise ontology based on agents and contexts

Dissertação de mest., Engenharia Informática, Faculdade de Ciências e Tecnologia, Universidade do Algarve, 2011Communication it is a fundamental organizational aspect but not always easy to achieve due to differences between the parts involved (distinct beliefs, backgrounds and needs). Poor communication can lead the organization to several problems like poor resource re-utilization, deficient inter-operability, underutilization of information, among others. These communication problems are translated into information flux problems (we do not use efficiently the information, we do not understand each other and we do not define problems or propose solutions adequately). Addressing these problems its crucial in every organization that understands the value that information has. Ontologies help us solve the previous situations. They provide a common understanding of a conceptualization of the study object so they allow us to model the study object and to describe it using the same language. Enterprise ontologies allow us model an organization like the enterprise using a unambiguous and commonly accepted terminology who define enterprise terms like activity, resource or process with which we can represent enterprise operation and manage information efficiently. Commonly, enterprise ontologies model formal elements of an organization, but since enterprises are composed by parts of different nature, informal elements emerge and create relations and different perspectives with respect to the rest of the enterprise components. This document study an agent based and context aware framework who encompasses both, formal and informal element of the enterprise. This work results on a formalized OWL ontology that will allow us to answer question with information inferred from the model

Nivel de conocimiento de internos rotativos de medicina en el aprendizaje basado en problemas para el diagnóstico y tratamiento de patologías frecuentes en pacientes que acuden al Hospital Provincial general de Latacunga año 2014. propuesta de una guía metodológica en aprendizaje basado en problemas

Medicine is one of the most difficult careers to learn for its continuous change, it was characterized by following an intensive pattern of basic science, it was gradually becoming in ineffective and inhumane way to prepare students, in view of the explosive growth of medical information and new technologies, in addition to the rapidly changing demands of professional practice, the teaching method had a Problem Based on learning (PBL) is one of the best proposal as a learning method in a medical career; A descriptive investigation on the knowledge level of rotary internal medicine in the ABP for the diagnosis and treatment of common diseases in patients attending the Latacunga General Hospital.La medicina una de las carreras más difíciles de aprender por su cambio continuo, se caracterizaba por seguir un patrón intensivo de clases expositivas de ciencias básicas, fue convirtiéndose gradualmente en una forma inefectiva e inhumana de preparar estudiantes, en vista del crecimiento explosivo de la información médica y las nuevas tecnologías, además de las demandas rápidamente cambiantes de la práctica profesional, el método del Aprendizaje Basado en Problemas (ABP) es una de las mejores propuesta como método de aprendizaje en la carrera de medicina; se realizó una investigación descriptiva transversal sobre el nivel de conocimiento de los internos rotativos de medicina en el ABP para el diagnóstico y tratamiento de patologías frecuentes en pacientes que acuden al Hospital Provincial General de Latacunga

Complete sequence of the genome of the human isolate of Andes virus CHI-7913: comparative sequence and protein structure analysis

Indexación: ScieloWe report here the complete genomic sequence of the Chilean human isolate of Andes virus CHI-7913. The S, M, and L genome segment sequences of this isolate are 1,802, 3,641 and 6,466 bases in length, with an overall GC content of 38.7%. These genome segments code for a nucleocapsid protein of 428 amino acids, a glycoprotein precursor protein of 1,138 amino acids and a RNA-dependent RNA polymerase of 2,152 amino acids. In addition, the genome also has other ORFs coding for putative proteins of 34 to 103 amino acids. The encoded proteins have greater than 98% overall similarity with the proteins of Andes virus isolates AH-1 and Chile R123. Among other sequenced Hantavirus, CHI-7913 is more closely related to Sin Nombre virus, with an overall protein similarity of 92%. The characteristics of the encoded proteins of this isolate, such as hydrophobic domains, glycosylation sites, and conserved amino acid motifs shared with other Hantavirus and other members of the Bunyaviridae family, are identified and discussed

Combined Holt-Winters and GA trained ANN approach for sensor validation and reconstruction: application to water demand flowmeters

© 2016 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other worksThis paper proposes a Double Seasonal Holt-Winters (DSHW) forecasting model with an auxiliary Artificial Neural Network (ANN) trained with a Genetic Algorithm (GA) to model the DSHW residuals. ANN complements and improves the DSHW prediction. The proposed model also includes an on-line validation and reconstruction mechanism useful to detect and correct missing and erroneous data. This mechanism also impacts improving the DSHW prediction accuracy and precision. The proposed model and validation mechanism are applied to predict the time series generated by two monitored flowmeters of two sectors of Barcelona's drinking water network (DWN). The accuracy and precision improvement of the proposed method with respect to standard DSHW and ARIMA approaches is provided.Accepted versio