Radiation effects on two‐dimensional materials

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/135401/1/pssa201600395_am.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/135401/2/pssa201600395.pd

Weed Control for Reduced Tillage Systems

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High throughput automated microbial bioreactor system used for clone selection and rapid scale-down process optimization

High throughput automated fermentation systems have become a useful tool in early bioprocess development. In this study, we investigated a 24 x 15 mL single use microbioreactor system, ambr 15f, designed for microbial culture. We compared the fed-batch growth and production capabilities of this system for two Escherichia coli strains, BL21 (DE3) and MC4100, and two industrially relevant molecules, hGH and scFv. In addition, different carbon sources were tested using bolus, linear or exponential feeding strategies, showing the capacity of the ambr 15f system to handle automated feeding. We used power per unit volume (P/V) as a scale criterion to compare the ambr 15f with 1 L stirred bioreactors which were previously scaled-up to 20 L with a different biological system, thus showing a potential 1,300 fold scale comparability in terms of both growth and product yield. By exposing the cells grown in the ambr 15f system to a level of shear expected in an industrial centrifuge, we determined that the cells are as robust as those from a bench scale bioreactor. These results provide evidence that the ambr 15f system is an efficient high throughput microbial system that can be used for strain and molecule selection as well as rapid scale-up. © 2017 The Authors Biotechnology Progress published by Wiley Periodicals, Inc. on behalf of American Institute of Chemical Engineers Biotechnol. Prog., 2017

The Land Conservation Plan for New Hampshire\u27s Coastal Watershed

Spanning 990 square miles and 46 towns, New Hampshire’s coastal watersheds harbor exceptional and irreplaceable natural, cultural, recreational and scenic resources (Figure 1). To advance the long-term protection of these resources, the State of New Hampshire, acting through the NH Coastal Program and the NH Estuaries Project, sought to develop a comprehensive, science-based land conservation plan for our coastal watersheds. The State engaged a partnership of The Nature Conservancy, Society for the Protection of New Hampshire Forests, Rockingham Planning Commission, and Strafford Regional Planning Commission to develop the plan. The New Hampshire Charitable Foundation’s Piscataqua Region supported this effort as a regional approach to setting land conservation priorities and strategies, and provided substantial matching funds. Southeastern New Hampshire is changing before our eyes. The region’s forests, wildlife habitat, clean water, and scenic vistas are increasingly threatened by sprawling development, roads, and other irreversible land use changes. Over the past 36 years, in Rockingham and Strafford Counties, an average of 2,230 acres per year has been converted from undeveloped land to a developed condition. And there is no indication that the pace of development will slow in the foreseeable future. The two Counties are projected to add more than 100,000 new residents from 2000 to 2025, and land values continue to rise steeply. With this conversion comes the loss of important natural resource values provided by undeveloped land, especially for plant and wildlife habitat, clean water, and other “ecological services.” To ensure a healthy environment into the future, it is essential that communities identify, retain, and protect the remaining undeveloped lands and waters that support the most important of these natural resource values and functions. Fortunately, it is not too late to protect the essential natural resources of Great Bay, Hampton Harbor, and the many important watersheds feeding into New Hampshire’s coastline. Thanks to the foresight and dedicated efforts of communities, citizens, conservation organizations and public agencies, more than nine percent of our coastal watersheds are permanently conserved. Many municipalities and communities have embraced land conservation through open space bonds, master plans, and local ordinances. New federal funds, such as the Coastal and Estuarine Land Conservation program, are available for conservation in the coastal watersheds. These protected lands and waters form the basis of a network of conservation areas that will help to safeguard our most critical natural resources over time. Now, more than ever, coastal New Hampshire communities need to ensure that they are making smart, enduring conservation investments in land protection and other effective local and regional strategies to have the greatest and most long-lasting beneficial impact on coastal Now, more than ever, coastal New Hampshire communities need to ensure that they are making smart, enduring conservation investments in land protection and other effective local and regional strategies to have the greatest and most long-lasting beneficial impact on coasta

Computer automation of ultrasonic testing

Report describes a prototype computer-automated ultrasonic system developed for the inspection of weldments. This system can be operated in three modes: manual, automatic, and computer-controlled. In the computer-controlled mode, the system will automatically acquire, process, analyze, store, and display ultrasonic inspection data in real-time. Flaw size (in cross-section), location (depth), and type (porosity-like or crack-like) can be automatically discerned and displayed. The results and pertinent parameters are recorded

The affinity purification and characterization of ATP synthase complexes from mitochondria.

The mitochondrial F₁-ATPase inhibitor protein, IF₁, inhibits the hydrolytic, but not the synthetic activity of the F-ATP synthase, and requires the hydrolysis of ATP to form the inhibited complex. In this complex, the α-helical inhibitory region of the bound IF₁ occupies a deep cleft in one of the three catalytic interfaces of the enzyme. Its N-terminal region penetrates into the central aqueous cavity of the enzyme and interacts with the γ-subunit in the enzyme's rotor. The intricacy of forming this complex and the binding mode of the inhibitor endow IF₁ with high specificity. This property has been exploited in the development of a highly selective affinity procedure for purifying the intact F-ATP synthase complex from mitochondria in a single chromatographic step by using inhibitor proteins with a C-terminal affinity tag. The inhibited complex was recovered with residues 1-60 of bovine IF₁ with a C-terminal green fluorescent protein followed by a His-tag, and the active enzyme with the same inhibitor with a C-terminal glutathione-S-transferase domain. The wide applicability of the procedure has been demonstrated by purifying the enzyme complex from bovine, ovine, porcine and yeast mitochondria. The subunit compositions of these complexes have been characterized. The catalytic properties of the bovine enzyme have been studied in detail. Its hydrolytic activity is sensitive to inhibition by oligomycin, and the enzyme is capable of synthesizing ATP in vesicles in which the proton-motive force is generated from light by bacteriorhodopsin. The coupled enzyme has been compared by limited trypsinolysis with uncoupled enzyme prepared by affinity chromatography. In the uncoupled enzyme, subunits of the enzyme's stator are degraded more rapidly than in the coupled enzyme, indicating that uncoupling involves significant structural changes in the stator region