A Survey of z ~ 6 Quasars in the Sloan Digital Sky Survey Deep Stripe. II. Discovery of Six Quasars at z AB>21

We present the discovery of six new quasars at z ~ 6 selected from the Sloan Digital Sky Survey (SDSS) southern survey, a deep imaging survey obtained by repeatedly scanning a stripe along the celestial equator. The six quasars are about 2 mag fainter than the luminous z ~ 6 quasars found in the SDSS main survey and 1 mag fainter than the quasars reported in Paper I. Four of them comprise a complete flux-limited sample at 21 < z_(AB) < 21.8 over an effective area of 195 deg^2. The other two quasars are fainter than z_(AB) = 22 and are not part of the complete sample. The quasar luminosity function at z ~ 6 is well described as a single power law Φ(L_(1450))α L^β_(1450) over the luminosity range –28 < M_(1450) < –25. The best-fitting slope β varies from –2.6 to –3.1, depending on the quasar samples used, with a statistical error of 0.3-0.4. About 40% of the quasars discovered in the SDSS southern survey have very narrow Lyα emission lines, which may indicate small black hole masses and high Eddington luminosity ratios, and therefore short black hole growth timescales for these faint quasars at early epochs

Cyclophilin A Controls Salmonella Internalization Levels and is Present at E. coli Actin‐Rich Pedestals

Salmonella enterica serovar Typhimurium (S. Typhimurium), enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC) commandeer the actin cytoskeleton of their host cells as a crucial step in their infectious processes. These pathogens depend on the injection of their own effectors directly into target host cells in order to usurp cellular signaling pathways that lead to morphological actin rearrangements in those cells. Here we show that the PPIase Cyclophilin A (CypA) is a novel component of S. Typhimurium-induced membrane ruffles and functions to restrict bacterial invasion levels, as in cells depleted of CypA, bacterial loads increase. We also demonstrate that CypA requires the EPEC effector Tir as well as pedestal formation for its recruitment to bacterial attachment sites and that its presence at pedestals also holds during EHEC infections. Finally, we demonstrate that CypA is found at lamellipodia; actin-rich structures at the leading edge of motile cells. Our findings further establish CypA as a component of dynamic actin-rich structures formed during bacterial infections and within cells in general

Global Gene Expression Profiling in Lung Tissues of Rat Exposed to Lunar Dust Particles

The Moon's surface is covered by a layer of fine, potential reactive dust. Lunar dust contain about 12% respirable very fine dust (less than 3 micrometers). The habitable area of any lunar landing vehicle and outpost would inevitably be contaminated with lunar dust that could pose a health risk. The purpose of the study is to analyze the dynamics of global gene expression changes in lung tissues of rats exposed to lunar dust particles. F344 rats were exposed for 4 weeks (6h/d; 5d/wk) in noseonly inhalation chambers to concentrations of 0 (control air), 2.1, 6.8, 21, and 61 mg/m3 of lunar dust. Animals were euthanized at 1 day and 13 weeks after the last inhalation exposure. After being lavaged, lung tissue from each animal was collected and total RNA was isolated. Four samples of each dose group were analyzed using Agilent Rat GE v3 microarray to profile global gene expression of 44K transcripts. After background subtraction, normalization, and log transformation, t tests were used to compare the mean expression levels of each exposed group to the control group. Correction for multiple testing was made using the method of Benjamini, Krieger, and Yekuteli (1) to control the false discovery rate. Genes with significant changes of at least 1.75 fold were identified as genes of interest. Both low and high doses of lunar dust caused dramatic, dosedependent global gene expression changes in the lung tissues. However, the responses of lung tissue to low dose lunar dust are distinguished from those of high doses, especially those associated with 61mg/m3 dust exposure. The data were further integrated into the Ingenuity system to analyze the gene ontology (GO), pathway distribution and putative upstream regulators and gene targets. Multiple pathways, functions, and upstream regulators have been identified in response to lunar dust induced damage in the lung tissue

The Sloan Digital Sky Survey Quasar Lens Search. II. Statistical lens sample from the third data release

We report the first results of our systematic search for strongly lensed quasars using the spectroscopically confirmed quasars in the Sloan Digital Sky Survey (SDSS). Among 46,420 quasars from the SDSS Data Release 3 (~4188 deg^2), we select a subsample of 22,683 quasars that are located at redshifts between 0.6 and 2.2 and are brighter than the Galactic extinction-corrected i-band magnitude of 19.1. We identify 220 lens candidates from the quasar subsample, for which we conduct extensive and systematic follow-up observations in optical and near-infrared wavebands, in order to construct a complete lensed quasar sample at image separations between 1" and 20" and flux ratios of faint to bright lensed images larger than 10^(−0.5). We construct a statistical sample of 11 lensed quasars. Ten of these are galaxy-scale lenses with small image separations (~ 1"-2") and one is a large separation (15") system which is produced by a massive cluster of galaxies, representing the first statistical sample of lensed quasars including both galaxy- and cluster-scale lenses. The Data Release 3 spectroscopic quasars contain an additional 11 lensed quasars outside the statistical sample

Overview of results from the Asian Seas International Acoustics Experiment in the East China Sea

Author Posting. © IEEE, 2004. This article is posted here by permission of IEEE for personal use, not for redistribution. The definitive version was published in IEEE Journal of Oceanic Engineering 29 (2004): 920-928, doi:10.1109/JOE.2005.843159.The Asian Seas International Acoustics Experiment (ASIAEX) included two major field programs, one in the South China Sea and the other in the East China Sea (ECS). This paper presents an overview of research results from ASIAEX ECS conducted between May 28 and June 9, 2001. The primary emphasis of the field program was shallow-water acoustic propagation, focused on boundary interaction and geoacoustic inversion. The study area's central point was located at 29/spl deg/ 40.67'N, 126/spl deg/ 49.39'E, which is situated 500 km east of the Chinese coastline off Shanghai. The acoustic and supporting environmental measurements are summarized, along with research results to date, and references to papers addressing specific issues in more detail are given.This work was supported by the U.S. Office of Naval Research under Code 321 OA and by sponsoring agencies within China. Primary guidance and sponsorship for ASIAEX East China Sea came from the U.S. Office of Naval Research and significant financial support was also received from sponsoring agencies within China

Hedgehog Pathway as a Potential Intervention Target in Esophageal Cancer

Esophageal cancer (EC) is an aggressive disease with a poor prognosis. Treatment resistance is a major challenge in successful anti-cancer therapy. Pathological complete response after neoadjuvant chemoradiation (nCRT) is low, thus requiring therapy optimization. The Hedgehog (HH) pathway has been implicated in therapy resistance, as well as in cancer stemness. This article focusses on the HH pathway as a putative target in the treatment of EC. Immunohistochemistry on HH members was applied to EC patient material followed by modulation of 3D-EC cell cultures, fluorescence-activated cell sorting (FACS), and gene expression analysis after HH pathway modulation. Sonic Hedgehog (SHH) and its receptor Patched1 (PTCH1) were significantly enriched in EC resection material of patients with microresidual disease (mRD) after receiving nCRT, compared to the control group. Stimulation with SHH resulted in an up-regulation of cancer stemness in EC sphere cultures, as indicated by increased sphere formation after sorting for CD44+/CD24- EC cancer stem-like cell (CSC) population. On the contrary, inhibiting this pathway with vismodegib led to a decrease in cancer stemness and both radiation and carboplatin resistance. Our results strengthen the role of the HH pathway in chemoradiotherapy resistance. These findings suggest that targeting the HH pathway could be an attractive approach to control CSCs.</p

A Survey of z~6 Quasars in the SDSS Deep Stripe. II. Discovery of Six Quasars at z_{AB}>21

We present the discovery of six new quasars at z~6 selected from the Sloan Digital Sky Survey (SDSS) southern survey, a deep imaging survey obtained by repeatedly scanning a stripe along the celestial equator. The six quasars are about two magnitudes fainter than the luminous z~6 quasars found in the SDSS main survey and one magnitude fainter than the quasars reported in Paper I (Jiang et al. 2008). Four of them comprise a complete flux-limited sample at 21<z_AB<21.8 over an effective area of 195 deg^2. The other two quasars are fainter than z_AB=22 and are not part of the complete sample. The quasar luminosity function at z~6 is well described as a single power law \Phi(L_{1450}) \propto L_{1450}^{\beta} over the luminosity range -28<M_{1450}<-25. The best-fitting slope \beta varies from -2.6 to -3.1, depending on the quasar samples used, with a statistical error of 0.3-0.4. About 40% of the quasars discovered in the SDSS southern survey have very narrow Lya emission lines, which may indicate small black hole masses and high Eddington luminosity ratios, and therefore short black hole growth time scales for these faint quasars at early epochs.Comment: Accepted for publication in A

Observations and Theoretical Implications of the Large Separation Lensed Quasar SDSS J1004+4112

We study the recently discovered gravitational lens SDSS J1004+4112, the first quasar lensed by a cluster of galaxies. It consists of four images with a maximum separation of 14.62''. The system has been confirmed as a lensed quasar at z=1.734 on the basis of deep imaging and spectroscopic follow-up observations. We present color-magnitude relations for galaxies near the lens plus spectroscopy of three central cluster members, which unambiguously confirm that a cluster at z=0.68 is responsible for the large image separation. We find a wide range of lens models consistent with the data, but they suggest four general conclusions: (1) the brightest cluster galaxy and the center of the cluster potential well appear to be offset by several kpc; (2) the cluster mass distribution must be elongated in the North--South direction, which is consistent with the observed distribution of cluster galaxies; (3) the inference of a large tidal shear (~0.2) suggests significant substructure in the cluster; and (4) enormous uncertainty in the predicted time delays between the images means that measuring the delays would greatly improve constraints on the models. We also compute the probability of such large separation lensing in the SDSS quasar sample, on the basis of the CDM model. The lack of large separation lenses in previous surveys and the discovery of one in SDSS together imply a mass fluctuation normalization \sigma_8=1.0^{+0.4}_{-0.2} (95% CL), if cluster dark matter halos have an inner slope -1.5. Shallower profiles would require higher values of \sigma_8. Although the statistical conclusion might be somewhat dependent on the degree of the complexity of the lens potential, the discovery is consistent with the predictions of the abundance of cluster-scale halos in the CDM scenario. (Abridged)Comment: 21 pages, 24 figures, 5 tables, accepted for publication in Ap

Listeria Monocytogenes Hijacks CD147 to Ensure Proper Membrane Protrusion Formation and Efficient Bacterial Dissemination

Efficient cell-to-cell transfer of Listeria monocytogenes (L. monocytogenes) requires the proper formation of actin-rich membrane protrusions. To date, only the host proteins ezrin, the binding partner of ezrin, CD44, as well as cyclophilin A (CypA) have been identified as crucial components for L. monocytogenes membrane protrusion stabilization and, thus, efficient cell-to-cell movement of the microbes. Here, we examine the classical binding partner of CypA, CD147, and find that this membrane protein is also hijacked by the bacteria for their cellular dissemination. CD147 is enriched at the plasma membrane surrounding the membrane protrusions as well as the resulting invaginations generated in neighboring cells. In cells depleted of CD147, these actin-rich structures appear similar to those generated in CypA depleted cells as they are significantly shorter and more contorted as compared to their straighter counterparts formed in wild-type control cells. The presence of malformed membrane protrusions hampers the ability of L. monocytogenes to efficiently disseminate from CD147-depleted cells. Our findings uncover another important host protein needed for L. monocytogenes membrane protrusion formation and efficient microbial dissemination