Influence of cold storage time on the softening prediction in Spring Bright nectarines

With Time-resolved Reflectance Spectroscopy (TRS) the maturity of nectarines at harvest can be assessed by measuring the absorption coefficient at 670 nm (µa 670) in the fruit flesh. A kinetic model has been developed linking the optical properties as measured by TRS with the models of µa 670 and firmness decay in shelf-life at 20°C, making the prediction of the softening time for individual fruit possible. In order to study the influence of cold storage time prior to shelf life on the softening prediction, 540 (year 2003) and 870 (year 2004) ‘Spring Bright’ nectarines were measured at harvest with TRS; then fruit were put in shelf life after various periods of cold storage at 0°C (4 and 10 d, year 2003; 6, 13 and 20 days, year 2004). During the 5-day period of shelf life at 20°C, fruit were analysed for firmness by pressure test after 30, 48, 54, 72, 78, 96, 102 and 120h in 2003 and after 36, 43, 62, 87, 108 and 135h in 2004. For each year and cold storage time, the parameters of the logistic model of softening as a function of µa 670 at harvest were computed. The cold storage up to 13 days did not significantly influence the estimates of the softening rate constant (kf), of the maximum firmness at minus infinite time (Fmax) and of parameter alpha (a) in both years, whereas parameter beta (ß) in 2003 significantly decreased from -1.867 at day 4 to -2.237 at day 10. The further 7 days of cold storage in 2004 significantly affected kf, which decreased from 0.00084 at days 6 and 13 to 0.00069 at day 20, and ß which increased from -2.395 at day 6 to -2.053 at day 20. Our results indicate that the cold storage time significantly influences the softening prediction of nectarines as the longer the cold storage, the lower the softening rat

Assessing the Harvest Maturity of Brazilian Mangoes

No clear criterion exists to determine the optimum time to harvest mango. Some empirical relations are used to assess maturity, such as shoulder development. Moreover, as a result of the typical growing conditions in tropical climates, a huge variation in maturity and ripeness exists, seriously hampering the export of fruit in the global chain. The consequence for consumers in western countries is that sometimes mangoes are overripe at the retailer, or have to be kept for several days, even weeks, to reach the edible state, provided they do not rot in the meantime. To ensure an edible quality, the chlorophyll content in the fruit flesh, measured at harvest by Time-resolved Reflectance Spectroscopy (TRS), could be used as a maturity criterion for mango fruit. Commercially grown fruit were harvested in Brazil and transported to Italy by plane. Fruits were measured using TRS at 630 nm for absorption coefficient (µa) and skin colour. The development of µa was followed on 60 fruits during 15 days of storage at 20°C. The remainders of fruit were used to measure firmness destructively. Absorption coefficient decreased during shelf life according to a logistic pattern, as expected for colour development. Taking the variation between the individual fruit into account, 72% of the variation was accounted for. Nevertheless, µa assessed at harvest could be converted into a biological shift factor (BSF), as an expression of the maturity at harvest of each individual fruit. This biological shift factor explained about 70% of the variation in firmness development in individual fruit. These preliminary results indicate that TRS methodology coupled with BSF theory could be useful in assessing maturity at harvest and assuring acceptable eating quality of mango

Sensory profiles of various stored fruit species are affected by maturity class assessed by time-resolved reflectance spectroscopy at harvest

The absorption coefficient measured at harvest at 670 nm (μa670) by timeresolved reflectance spectroscopy (TRS) is a non-destructive maturity index used to evaluate the biological age of fruit, i.e., the fruit maturity stage. The μa670 was successfully used to classify nectarine, apple and mango fruit into maturity classes each one having distinctive ripening behaviors. Aiming at studying the influence of TRS maturity class assessed at harvest on the sensory profiles of various fruit species after storage, 'Jonagored' apples, 'Abbé Fétel' pears, 'Morsiani 90' nectarines and 'Spring Belle' peaches were measured at harvest by TRS at 670 nm, ranked on the basis of decreasing μa670 (increasing maturity) and classified as less (LeM), medium and more (MoM) mature. Then fruit were randomized into batches of 30 fruits, each one corresponding to a storage atmosphere (apples: CA, NA; pears: CA, NA, DCA) or to a storage temperature (peaches, nectarines: 0, 4°C). After storage, fruit were put in shelf life at 20°C to reach the ripening degree for consumption. Sensory analyses (QDA profiles) were carried out on LeM and MoM fruit using a panel of 10 assessors. Our results indicate that, for all the species, besides the influence of storage conditions, there was also a great influence of TRS maturity classification at harvest. MoM apples and pears developed physiological disorders such as mealiness and graininess when stored in NA, whereas showed well balanced sensory characteristics when stored in CA and DCA, becoming after storage soft, juicy, sweet, but sour enough. MoM nectarines stored both at 0 and 4°C became woolly, while the LeM ones stored at 0°C developed the best sensory characteristics. MoM peaches stored at 0°C showed the best sensory profile and the LeM ones stored at 4°C the worst. So TRS maturity classification at harvest can give indications on the best storage conditions in order to obtain fruit with sensory characteristics than can satisfy the consumer

The antiquity of hydrocephalus: the first full palaeo-neuropathological description

The Pathology Museum of the University of Florence houses a rich collection of anatomical specimens and over a hundred waxworks portraying pathological conditions occurring in the nineteenth century, when the museum was established. Clinical and autopsy findings of these cases can still be retrieved from the original museum catalogue, offering a rare opportunity for retrospective palaeo-pathological diagnostics. We present a historical case of severe hydrocephalus backed by modern-day anthropological, radiological and molecular analyses conducted on the skeleton of an 18-month-old male infant deceased in 1831. Luigi Calamai (1796-1851), a wax craftsman of La Specola workshop in Florence, was commissioned to create a life-sized wax model of the child's head, neck and upper thorax. This artwork allows us to appreciate the cranial and facial alterations determined by 30 lb of cerebrospinal fluid (CSF) accumulated within the cerebral ventricular system. Based on the autopsy report, gross malformations of the neural tube, tumours and haemorrhage could be excluded. A molecular approach proved helpful in confirming sex. We present this case as the so-far most compelling case of hydrocephalus in palaeo-pathological research

Modeling mango ripening during shelf life based on pulp color nondestructively measured by time-resolved reflectance spectroscopy

Non-destructive techniques could help mango growers to pick fruit at the proper maturity degree and to monitor fruit quality during storage and marketing to satisfy the consumer expectations. The absorption coefficient non-destructively measured at 540 nm (μa540) by time-resolved reflectance spectroscopy (TRS) was shown to be a maturity index for mango fruit and was highly correlated with pulp color parameters and carotenoids content. The aim of this work was to model μa540 using the biological shift factor theory to verify if μa540 is able to assess the maturity degree of individual mango fruit and then to use μa540 to model pulp color in order to relate μa540 to an important index of mango ripening. Mango fruit (Mangifera indica L. cv Tommy Atkins) at commercial maturity for ship and air transport maturities, were measured by TRS, ranked according to decreasing μa540 (decreasing maturity), randomized into six batches per transport maturity and analyzed for μa540 and pulp color parameters after 0, 1, 2, 5, 6 and 7 days of shelf life at 20 °C. The μa540 as a function of biological shift factor increased during ripening following a logistic/exponential model (R2adj=99%) with a faster rate in less mature fruit than in more mature ones. The changes in pulp color during mango ripening depended on fruit maturity (i.e., μa540) and on time of shelf life at 20 °C. By converting the μa540 into the biological shift factor it was possible to model the increasing trend of a*, b*, C*, and IY and the decreasing trend of L* and h° during the shelf life period explaining 91.2–99.8% of the variation and to differentiate mango fruit according to their biological age. Similarly to μa540, color changes occurred earlier in more mature fruit and later in less mature ones with the same pattern in time. There is a synchronization between changes of μa540 and changes of a*, b*, C* and yellowness during ripening in mango fruit which allows to use μa540 to sort fruit according to their maturity degree and then to optimize fruit management along the supply chain

Maturação de tubérculos da cultivar de batata BRS Ana.

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Matemática, Literatura & Aulas

A aprendizagem da Matemática não ocorre por mera transmissão de saberes do professor para os alunos. A ideia de que o conhecimento pode passar linearmente de uma cabeça – daquele que sabe – para outra(s) cabeça(s) – para aqueles que querem saber –, pelas palavras ditas e escritas, não tem qualquer correspondência com a realidade. A aprendizagem é um processo adaptativo, simultaneamente individual e coletivo, baseado na ação e na reflexão, no qual a comunicação tem um papel fundamental, na medida que permite estabelecer ligação entre as pessoas

HER2-positive male breast cancer: An update

none7noAlthough rare, male breast cancer (MBC) remains a substantial cause for morbidity and mortality in men. Based on age frequency distribution, age-specific incidence rate pattern, and prognostic factor profiles, MBC is considered similar to postmenopausal breast cancer (BC). Compared with female BC (FBC), MBC cases are more often hormonal receptor (estrogen receptor/progesterone receptor [ER/PR]) positive and human epidermal growth factor receptor 2 (HER2) negative. Treatment of MBC patients follows the same indications as female postmenopausal with surgery, systemic therapy, and radiotherapy. To date, ER/PR and HER2 status provides baseline predictive information used in selecting optimal adjuvant/neoadjuvant therapy and in the selection of therapy for recurrent or metastatic disease. HER2 represents a very interesting molecular target and a number of compounds (trastuzumab [Herceptin®; F. Hoffmann-La Roche, Basel, Switzerland] and lapatinib [Tykerb®, GlaxoSmithKline, London, UK]) are currently under clinical evaluation. Particularly, trastuzumab, a monoclonal antibody which selectively binds the extracellular domain of HER2, has become an important therapeutic agent for women with HER2-positive (HER2+) BC. Currently, data regarding the use of trastuzumab in MBC patients is limited and only few case reports exist. In all cases, MBC patients received trastuzumab concomitantly with other drugs and no severe toxicity above grade 3 was observed. However, MBC patients that would be candidate for trastuzumab therapy (ie, HER2+/ER+ or HER2+/ER- MBCs) represent only a very small percentage of MBC cases. This is noteworthy, when taking into account that trastuzumab is an important and expensive component of systemic BC therapy. Since there is no data supporting the fact that response to therapy is different for men or women, we concluded that systemic therapy in MBC should be considered on the same basis as for FBC. Particularly in male patients, trastuzumab should be considered exclusively for advanced disease or high-risk HER2+ early BCs. On the other hand, lapatinib (Tykerb), a novel oral dual tyrosine kinase inhibitor that targets both HER2 and epidermal growth factor receptor, may represent an interesting and promising therapeutic agent for trastuzumab-resistant MBC patients.openOttini L.; Capalbo C.; Rizzolo P.; Silvestri V.; Bronte G.; Rizzo S.; Russo A.Ottini, L.; Capalbo, C.; Rizzolo, P.; Silvestri, V.; Bronte, G.; Rizzo, S.; Russo, A

Patterns of genomic instability in gastric cancer: clinical implications and perspectives.

In gastric cancer (GC) the loss of genomic stability represents a key molecular step that occurs early in the carcinogenesis process and creates a permissive environment for the accumulation of genetic and epigenetic alterations in tumor suppressor genes and oncogenes. It is widely accepted that GC can follow at least two major genomic instability pathways, microsatellite instability (MSI) and chromosome instability (CIN). MSI is responsible for a well-defined subset of GCs. CIN represents a more common pathway comprising heterogeneous subsets of GC. In addition to MSI and CIN, the CpG islands methylator phenotype (CIMP) plays an important role in gastric carcinogenesis. CIMP may lead to the transcriptional silencing of various genes in gastric carcinogenesis. Intriguingly, more recently in addition to CpG island hypermethylation, a global DNA demethylation, that precedes genomic damage, has been observed in GC. Thus, epigenetic alterations may play a relevant role in gastric carcinogenesis as alternative mechanisms. Evidence suggests that although MSI, CIN and CIMP phenotypes can be distinguished from one another, there might be some degree of overlap. This review describes our current knowledge of the instability pathways in gastric carcinogenesis and the potential clinical applications for different forms of genomic instability in GC