X-Ray Quantitative Evaluation of Multi-Layered Objects from Few Projections: A Multiresolution Technique

In this paper, we present the results of a study[1] motivated by the radiographic inspection of solder joints on double-sided printed circuits. Our goal was to obtain quantitative information about the structures in these planar objects, taking into account the acquisition geometry and time constraints. On one hand, we must limit the acquisition angle for projections at a value of ± 50 degrees with respect to the normal to the object plane, in order to limit the attenuation of the material crossed by X-rays. On the other hand, we must limit the number of projections in order to reduce as far as possible the acquisition and processing times

Compact cryogenic Kerr microscope for time-resolved studies of electron spin transport in microstructures

A compact cryogenic Kerr microscope for operation in the small volume of high-field magnets is described. It is suited for measurements both in Voigt and Faraday configuration. Coupled with a pulsed laser source, the microscope is used to measure the time-resolved Kerr rotation response of semiconductor microstructures with ~1 micron spatial resolution. The microscope was designed to study spin transport, a critical issue in the field of spintronics. It is thus possible to generate spin polarization at a given location on a microstructure and probe it at a different location. The operation of the microscope is demonstrated by time-resolved measurements of micrometer distance diffusion of spin polarized electrons in a GaAs/AlGaAs heterojunction quantum well at 4.2 K and 7 Tesla

Suppressed spin dephasing for 2D and bulk electrons in GaAs wires due to engineered cancellation of spin-orbit interaction terms

We report a study of suppressed spin dephasing for quasi-one-dimensional electron ensembles in wires etched into a GaAs/AlGaAs heterojunction system. Time-resolved Kerr-rotation measurements show a suppression that is most pronounced for wires along the [110] crystal direction. This is the fingerprint of a suppression that is enhanced due to a strong anisotropy in spin-orbit fields that can occur when the Rashba and Dresselhaus contributions are engineered to cancel each other. A surprising observation is that this mechanisms for suppressing spin dephasing is not only effective for electrons in the heterojunction quantum well, but also for electrons in a deeper bulk layer.Comment: 5 pages, 3 figure

Evidence for a black hole in the historical X-ray transient A 1524-61 (=KY TrA)

We present VLT spectroscopy, high-resolution imaging and time-resolved photometry of KY TrA, the optical counterpart to the X-ray binary A 1524-61. We perform a refined astrometry of the field, yielding improved coordinates for KY TrA and the field star interloper of similar optical brightness that we locate $0.64 \pm 0.04$ arcsec SE. From the spectroscopy, we refine the radial velocity semi-amplitude of the donor star to $K_2 = 501 \pm 52$ km s$^{-1}$ by employing the correlation between this parameter and the full-width at half-maximum of the H$\alpha$ emission line. The $r$-band light curve shows an ellipsoidal-like modulation with a likely orbital period of $0.26 \pm 0.01$ d ($6.24 \pm 0.24$ h). These numbers imply a mass function $f(M_1) = 3.2 \pm 1.0$ M$_\odot$. The KY TrA de-reddened quiescent colour $(r-i)_0 = 0.27 \pm 0.08$ is consistent with a donor star of spectral type K2 or later, in case of significant accretion disc light contribution to the optical continuum. The colour allows us to place a very conservative upper limit on the companion star mass, $M_2 \leq 0.94$ M$_\odot$, and, in turn, on the binary mass ratio, $q = M_2/M_1 \leq 0.31$. By exploiting the correlation between the binary inclination and the depth of the H$\alpha$ line trough, we establish $i = 57 \pm 13$ deg. All these values lead to a compact object and donor mass of $M_1 = 5.8^{+3.0}_{-2.4}$ M$_\odot$ and $M_2 = 0.5 \pm 0.3$ M$_\odot$, respectively, thus confirming the black hole nature of the accreting object. In addition, we estimate a distance toward the system of $8.0 \pm 0.9$ kpc.Comment: 7 pages, 5 figure

Optical probing of spin dynamics of two-dimensional and bulk electrons in a GaAs/AlGaAs heterojunction system

We present time-resolved Kerr rotation measurements of electron spin dynamics in a GaAs/AlGaAs heterojunction system that contains a high-mobility two-dimensional electron gas (2DEG). Due to the complex layer structure of this material the Kerr rotation signals contain information from electron spins in three different layers: the 2DEG layer, a GaAs epilayer in the heterostructure, and the underlying GaAs substrate. The 2DEG electrons can be observed at low pump intensities, using that they have a less negative g-factor than electrons in bulk GaAs regions. At high pump intensities, the Kerr signals from the GaAs epilayer and the substrate can be distinguished when using a barrier between the two layers that blocks intermixing of the two electron populations. This allows for stronger pumping of the epilayer, which results in a shift of the effective g-factor. Thus, three populations can be distinguished using differences in g-factor. We support this interpretation by studying how the spin dynamics of each population has its unique dependence on temperature, and how they correlate with time-resolved reflectance signals.Comment: 14 pages, 7 figure

Black hole mass and spin measurements through the Relativistic Precession Model: XTE J1859+226

The X-ray light curves of accreting black holes and neutron stars in binary systems show various types of quasi-periodic oscillations (QPOs), the origin of which is still debated. The Relativistic Precession Model identifies the QPO frequencies with fundamental time scales from General Relativity, and has been proposed as a possible explanation of certain types of such oscillations. Under specific conditions (i.e., the detection of a particular QPOs triplet) such a model can be used to obtain self-consistent measurements of the mass and spin of the compact object. So far this has been possible only in the black hole binary GRO J1655-40. In the RXTE/PCA data from the 1999-2000 outburst of the black hole transient XTE J1859+226 we found a QPO triplet, and used the the Relativistic Precession Model to obtain high-precision measurements of the black hole mass and spin - M = (7.85+/-0.46) Msun, a* = 0.149+/-0.005 - the former being consistent with the most recent dynamical mass determination from optical measurements. Similarly to what has been already observed in other black hole systems, the frequencies of the QPOs and broad-band noise components match the general relativistic frequencies of particle motion close to the compact object predicted by the model. Our findings confirm previous results and further support the validity of the Relativistic Precession Model, which is the only electromagnetic-measurement-based method that so far has consistently yielded spins close to those from the gravitational waves produced by merging binary black holes.Comment: 8 pages, 5 figures, accepted for publication in MNRA

The orbital period, black hole mass and distance to the X-ray transient GRS 1716-249 (=N Oph 93)

We present evidence for a 0.278(8) d (=6.7 h) orbital period in the X-ray transient GRS 1716-249 (=N Oph 93), based on a superhump modulation detected during the 1995 mini-outburst plus ellipsoidal variability in quiescence. With a quiescent magnitude of r=23.19+-0.15 N Oph 93 is too faint to warrant a full dynamical study through dedicated time-resolved spectroscopy. Instead, we apply the FWHM-K2 correlation to the disc Halpha emission line detected in Gran Telescopio Canarias spectra and obtain K2=521+-52 km/s. This leads to a mass function f(M)=4.1+-1.2 Msun, thus indicating the presence of a black hole in this historic X-ray transient. Furthermore, from the depth of the Halpha trough and the quiescent light curve we constrain the binary inclination to i=61+-15 deg, while the detection of superhumps sets an upper limit to the donor to compact star mass ratio q=M2/M1<=0.25. Our de-reddened (r-i) colour is consistent with a ~K6 main sequence star that fills its Roche lobe in a 0.278 d orbit. Using all this information we derive a compact object mass M1=6.4+3.2-2.0 Msun at 68 per cent confidence. We also constrain the distance to GRS 1716-249 to 6.9+-1.1 kpc, placing the binary ~0.8 kpc above the Galactic Plane, in support of a large natal kick.Comment: Accepted for publication in MNRAS, 12 pages, 9 figures, 2 Table

Phosphorylation of Syntaxin‐1a by casein kinase 2α (CK2α) regulates presynaptic vesicle exocytosis from the reserve pool

The t-soluble NSF-attachment protein receptor protein Syntaxin-1a (Stx-1a) is abundantly expressed at pre-synaptic terminals where it plays a critical role in the exocytosis of neurotransmitter-containing synaptic vesicles. Stx-1a is phosphorylated by Casein kinase 2α (CK2α) at Ser14, which has been proposed to regulate the interaction of Stx-1a and Munc-18 to control of synaptic vesicle priming. However, the role of CK2α in synaptic vesicle dynamics remains unclear. Here, we show that CK2α over-expression reduces evoked synaptic vesicle release. Furthermore, shRNA-mediated knockdown of CK2α in primary hippocampal neurons strongly enhanced vesicle exocytosis from the reserve pool, with no effect on the readily releasable pool of primed vesicles. In neurons in which endogenous Stx-1a was knocked down and replaced with a CK2α phosphorylation-deficient mutant, Stx-1a(D17A), vesicle exocytosis was also increased. These results reveal a previously unsuspected role of CK2α phosphorylation in specifically regulating the reserve synaptic vesicle pool, without changing the kinetics of release from the readily releasable pool

Structural and Mutational Analysis of Functional Differentiation between Synaptotagmins-1 and -7

Synaptotagmins are known to mediate diverse forms of Ca2+-triggered exocytosis through their C2 domains, but the principles underlying functional differentiation among them are unclear. Synaptotagmin-1 functions as a Ca2+ sensor in neurotransmitter release at central nervous system synapses, but synaptotagmin-7 does not, and yet both isoforms act as Ca2+ sensors in chromaffin cells. To shed light into this apparent paradox, we have performed rescue experiments in neurons from synaptotagmin-1 knockout mice using a chimera that contains the synaptotagmin-1 sequence with its C2B domain replaced by the synaptotagmin-7 C2B domain (Syt1/7). Rescue was not achieved either with the WT Syt1/7 chimera or with nine mutants where residues that are distinct in synaptotagmin-7 were restored to those present in synaptotagmin-1. To investigate whether these results arise because of unique conformational features of the synaptotagmin-7 C2B domain, we determined its crystal structure at 1.44 Å resolution. The synaptotagmin-7 C2B domain structure is very similar to that of the synaptotagmin-1 C2B domain and contains three Ca2+-binding sites. Two of the Ca2+-binding sites of the synaptotagmin-7 C2B domain are also present in the synaptotagmin-1 C2B domain and have analogous ligands to those determined for the latter by NMR spectroscopy, suggesting that a discrepancy observed in a crystal structure of the synaptotagmin-1 C2B domain arose from crystal contacts. Overall, our results suggest that functional differentiation in synaptotagmins arises in part from subtle sequence changes that yield dramatic functional differences