Interaction of Green Polymer Blend of Modified Sodium Alginate and Carboxylmethyl Cellulose Encapsulation of Turmeric Extract

Turmeric extract (tmr) loaded nanoparticles were prepared by crosslinking modified carboxylmethyl cellulose (CMC) and modified sodium alginate (SA) with calcium ions, in a high pressure homogenizer. The FTIR spectra of CMC and SA were affected by blending due to hydrogen bonding. The negative zeta potential increased in magnitude with CMC content. The smallest nanoparticles were produced with a 10 : 5 SA/CMC blend. Also the release rates of the extract loading were measured, with model fits indicating that the loading level affected the release rate through nanoparticle structure. The 10 : 5 SA/CMC blend loading with tmr and pure tmr showed a good % growth inhibition of colon cancer cells which indicate that tmr in the presence of curcumin in tmr retains its anticancer activity even after being loaded into SA/CMC blend matrix

Low Cost DNA Molecular Weight Marker: Primer-Directed Synthesis from pGEM-T Easy Vector

A low cost DNA molecular weight marker was produced by a marker primer-directed synthetic method using pGEM-T Easy vector as the DNA template. Seven primers were used to amplify eight different DNA fragments, which were 150, 300, 375, 500, 700, 1,000, 1,200 and 1,625 bp, from bacterial culture containing pGEM-T Easy vector. Polymerase chain reactions (PCR) for all marker loci required the same optimal annealing temperature, which allowed all the PCR to be completed in a single run. To obtain the molecular weight marker, the PCR product of each locus was mixed together and directly used as marker without any further purification. This custom made molecular weight marker was found to be approximately 17 to 49 times less expensive than other commercial 100 bp DNA ladder markers. Graphical abstrac