753 research outputs found
Unraveling the Physiological Roles of the Cyanobacterium Geitlerinema sp. BBD and Other Black Band Disease Community Members through Genomic Analysis of a Mixed Culture
Black band disease (BBD) is a cyanobacterial-dominated polymicrobial mat that propagates on and migrates across coral surfaces, necrotizing coral tissue. Culture-based laboratory studies have investigated cyanobacteria and heterotrophic bacteria isolated from BBD, but the metabolic potential of various BBD microbial community members and interactions between them remain poorly understood. Here we report genomic insights into the physiological and metabolic potential of the BBD-associated cyanobacterium Geitlerinema sp. BBD 1991 and six associated bacteria that were also present in the non-axenic culture. The essentially complete genome of Geitlerinema sp. BBD 1991 contains a sulfide quinone oxidoreductase gene for oxidation of sulfide, suggesting a mechanism for tolerating the sulfidic conditions of BBD mats. Although the operon for biosynthesis of the cyanotoxin microcystin was surprisingly absent, potential relics were identified. Genomic evidence for mixed-acid fermentation indicates a strategy for energy metabolism under the anaerobic conditions present in BBD during darkness. Fermentation products may supply carbon to BBD heterotrophic bacteria. Among the six associated bacteria in the culture, two are closely related to organisms found in culture-independent studies of diseased corals. Their metabolic pathways for carbon and sulfur cycling, energy metabolism, and mechanisms for resisting coral defenses suggest adaptations to the coral surface environment and biogeochemical roles within the BBD mat. Polysulfide reductases were identified in a Flammeovirgaceae genome (Bacteroidetes) and the sox pathway for sulfur oxidation was found in the genome of a Rhodospirillales bacterium (Alphaproteobacteria), revealing mechanisms for sulfur cycling, which influences virulence of BBD. Each genomic bin possessed a pathway for conserving energy from glycerol degradation, reflecting adaptations to the glycerol-rich coral environment. The presence of genes for detoxification of reactive oxygen species and resistance to antibiotics suggest mechanisms for combating coral defense strategies. This study builds upon previous research on BBD and provides new insights into BBD disease etiology
Molecular systematic perspectives on biome origins and dynamics
invited commentary on the following article:De-Nova JA, Medina R, Montero JC, Weeks A, Rosell JA, Olson ME, Eguiarte
LE, Magallo ́n S. 2011. Insights into the historical construction of species-rich Mesoamerican seasonally dry tropical forests: the diversification of Bursera
(Burseraceae, Sapindales). New Phytologist 193: 276–287.Phylogenetic reconstructions of diverse tropical groups have yielded biological insights that extend well beyond the field of systematics, illuminating such topics as biome assembly and plant–insect coevolution. In this issue of New Phytologist, De-Nova et al. (pp. 276–287) present a rigorous time-calibrated phylogeny of the Neotropical tree genus Bursera in order to estimate lineage ages, and to investigate how geography and ecology have structured its species diversification. Because Bursera is a dominant component of Mesoamerican seasonally dry tropical forests (SDTFs), the study provides evidence relating to the origin and expansion of this important biome. The study also sets the record straight on the age of a well-known plant– herbivore interaction.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/88158/1/Dick&Pennington2012.pdf-
The right posterior paravermis and the control of language interference
Auditory and written language in humans' comprehension necessitates attention to the message of interest and suppression of interference from distracting sources. Investigating the brain areas associated with the control of interference is challenging because it is inevitable that activation of the brain regions that control interference co-occurs with activation related to interference per se. To isolate the mechanisms that control verbal interference, we used a combination of structural and functional imaging techniques in Italian and German participants who spoke English as a second language. First, we searched structural MRI images of Italian participants for brain regions in which brain structure correlated with the ability to suppress interference from the unattended dominant language (Italian) while processing heard sentences in their weaker language (English). This revealed an area in the posterior paravermis of the right cerebellum in which gray matter density was higher in individuals who were better at controlling verbal interference. Second, we found functional activation in the same region when our German participants made semantic decisions on written English words in the presence of interference from unrelated words in their dominant language (German). This combination of structural and functional imaging therefore highlights the contribution of the right posterior paravermis to the control of verbal interference. We suggest that the importance of this region for language processing has previously been missed because most fMRI studies limit the field of view to increase sensitivity, with the lower part of the cerebellum being the region most likely to be excluded
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Cold-Crucible Design Parameters for Next Generation HLW Melters
The cold-crucible induction melter (CCIM) design eliminates many materials and operating constraints inherent in joule-heated melter (JHM) technology, which is the standard for vitrification of high-activity wastes worldwide. The cold-crucible design is smaller, less expensive, and generates much less waste for ultimate disposal. It should also allow a much more flexible operating envelope, which will be crucial if the heterogeneous wastes at the DOE reprocessing sites are to be vitrified. A joule-heated melter operates by passing current between water-cooled electrodes through a molten pool in a refractory-lined chamber. This design is inherently limited by susceptibility of materials to corrosion and melting. In addition, redox conditions and free metal content have exacerbated materials problems or lead to electrical short-circuiting causing failures in DOE melters. In contrast, the CCIM design is based on inductive coupling of a water-cooled high-frequency electrical coil with the glass, causing eddycurrents that produce heat and mixing. A critical difference is that inductance coupling transfers energy through a nonconductive solid layer of slag coating the metal container inside the coil, whereas the jouleheated design relies on passing current through conductive molten glass in direct contact with the metal electrodes and ceramic refractories. The frozen slag in the CCIM design protects the containment and eliminates the need for refractory, while the corrosive molten glass can be the limiting factor in the JH melter design. The CCIM design also eliminates the need for electrodes that typically limit operating temperature to below 1200 degrees C. While significant marketing claims have been made by French and Russian technology suppliers and developers, little data is available for engineering and economic evaluation of the technology, and no facilities are available in the US to support testing. A currently funded project at the Idaho National Engineering and Environmental Laboratory (INEEL), is providing preliminary data on the CCIM technology using a small laboratory unit at the Khlopin Radium Institute in St. Petersburg Russia with INEEL Sodium Bearing Waste surrogate. The task includes both the baseline borosilicate glass and a new iron-phosphate glass developed at the University of Missouri-Rolla, which may offer significant advantages in compatibility with greater concentrations of highly refractory oxides. This project is integrating two disparate advances to develop a system with strong potential for benefit to the Department of Energy. Collaborative development of basic physical parameter data on the CCIM using promising glass formulations is being conducted by University of Missouri - Rolla, Russian and American researchers
Are Amphipod invaders a threat to the regional biodiversity? Conservation prospects for the Loire River
The impact of invasions on local biodiversity is well established, but their impact on regional biodiversity has so far been only sketchily documented. To address this question, we studied the impact at various observation scales (ranging from the microhabitat to the whole catchment) of successive arrivals of non-native amphipods on the amphipod assemblage of the Loire River basin in France. Amphipod assemblages were studied at 225 sites covering the whole Loire catchment. Non-native species were dominant at all sites in the main channel of the Loire River, but native species were still present at most of the sites. We found that the invaders have failed to colonize most of tributaries of the Loire River. At the regional scale, we found that since the invaders first arrived 25 years ago, the global amphipod diversity has increased by 33% (from 8 to 12 species) due to the arrival of non-native species. We discuss the possibility that the lack of any loss of biodiversity may be directly linked to the presence of refuges at the microhabitat scale in the Loire channel and in the tributaries, which invasive species have been unable to colonize. The restoration of river quality could
increase the number of refuges for native species, thus
reducing the impact of invader
Implementation of NICE Clinical Guideline 95 for assessment of stable chest pain in a rapid access chest pain clinic reduces the mean number of investigations and cost per patient
Objective In 2010, the National Institute for Health and Care Excellence (NICE) in the UK published Clinical Guideline 95 (CG95) advocating risk stratification of patients using ‘CADScore’ to guide appropriate cardiac investigations for chest pain of recent onset. Implementation of the guideline in the University College London Hospitals NHS Foundation Trust was evaluated to see if it led to a reduction in the average cost of the diagnostic journey per patient and fewer investigations per patient in order to confirm a diagnosis.
Methods This was a single centre study at a Tertiary Centre in Central London. The investigative journey for each patient presenting to the Rapid Access Chest Pain Clinic (RACPC) at University College London Hospitals NHS Foundation Trust was recorded. Retrospective analysis on this data was performed.
Results Data for 4968 patients presenting to the RACPC from 2004 to 2012 was analysed and a size-matched cohort of 1503 patients preimplementation and postimplementation of the guidelines was compared. The mean cost of investigations postimplementation was £291.83 as compared to £319.54 preimplementation of the guidelines despite higher costs associated with some of the recommended initial investigations. The mean number of tests per patient postguidelines was 0.78 compared to 0.97 for preguidelines. An approximate twofold increase in patients not requiring tests was seen post-CG95 implementation (245 pre-CG95 vs 476 post-CG95)
Digital interventions in alcohol and drug prevention, treatment and recovery: Systematic maps of international research and interventions available in England
Executive Summary
Background
Digital interventions in alcohol and drug prevention, treatment and recovery have the
potential to overcome barriers faced by non-digital interventions. However, we lack a
clear understanding of the types of digital interventions that have been evaluated and
where gaps in the evidence base exist. We also need to understand the effectiveness of
different types of digital alcohol and drug interventions for various population groups.
Further, we do not know which digital alcohol and drug interventions are being used
in England, and whether the interventions in use align with those that have been
evaluated.
Research questions
To address the above concerns, we sought to address the following questions:
• RQ1: What is the possible range of digital alcohol and drug interventions?
• RQ2: Which types of digital alcohol and drug interventions are currently
available for use in England?
• RQ3: What systematic reviews provide findings for digital alcohol and drug
intervention strategies within a prevention/treatment/recovery pathway?
• RQ4: Which types of digital alcohol and drug interventions have been evaluated
in primary research?
• RQ5: To what extent does the evaluation evidence overlap with digital alcohol
and drug interventions that are currently available for use in England?
• RQ6: What evidence is there that certain types of digital alcohol and drug
interventions are (cost-) effective or ineffective for specific population groups
or in particular contexts?
This report covers our findings in relation to questions RQ1 - RQ5. Based on these
findings we also provide suggestions as to what could be the focus of further work to
answer RQ6.
Methods
To address RQ1 an initial typology was drafted, adapting and building on existing
typologies of digital interventions. Through this process it became clear to OHID/PHE
that a pathway, presenting a route through services, with different types of
interventions recommended for use at different times would be more helpful than a
typology of intervention characteristics. This pathway was then developed by OHID/PHE and trialled by the research team, with refinements made over time with
discussions between the study team and PHE.
To address RQ2 we contacted people in England in 2019, who were involved in
developing, commissioning, prescribing, recommending or evaluating digital
alcohol/drug interventions. Using an online survey, we asked them to describe the
interventions they were involved with.
To address RQ3, RQ4 and RQ5 we conducted systematic searching and screening to
identify and describe existing systematic reviews (RQ3) and primary studies (RQ4).
Included systematic reviews were appraised for quality and detailed information was
extracted from full reports. For primary studies we extracted basic details using the
information contained within the title and abstract.
The pathway developed for RQ1 was employed to code and describe the nature of
available interventions (RQ2), systematic reviews (RQ3) and primary studies (RQ4).
EPPI-Mapper software was used to produce online interactive maps to visually display
the findings
Neogene origins and implied warmth tolerance of Amazon tree species
Tropical rain forest has been a persistent feature in South America for at least 55 million years. The future of the contemporary Amazon forest is uncertain, however, as the region is entering conditions with no past analogue, combining rapidly increasing air temperatures, high atmospheric carbon dioxide concentrations, possible extreme droughts, and extensive removal and modification by humans. Given the long-term Cenozoic cooling trend, it is unknown whether Amazon forests can tolerate air temperature increases, with suggestions that lowland forests lack warm-adapted taxa, leading to inevitable species losses. In response to this uncertainty, we posit a simple hypothesis: the older the age of a species prior to the Pleistocene, the warmer the climate it has previously survived, with Pliocene (2.6-5 Ma) and late-Miocene (8-10 Ma) air temperature across Amazonia being similar to 2100 temperature projections under low and high carbon emission scenarios, respectively. Using comparative phylogeographic analyses, we show that 9 of 12 widespread Amazon tree species have Pliocene or earlier lineages (>2.6 Ma), with seven dating from the Miocene (>5.6 Ma) and three >8 Ma. The remarkably old age of these species suggest that Amazon forests passed through warmth similar to 2100 levels and that, in the absence of other major environmental changes, near-term high temperature-induced mass species extinction is unlikely
Four priority areas to advance invasion science in the face of rapid environmental change
Unprecedented rates of introduction and spread of non-native species pose burgeoning challenges to biodiversity, natural resource management, regional economies, and human health. Current biosecurity efforts are failing to keep pace with globalization, revealing critical gaps in our understanding and response to invasions. Here, we identify four priority areas to advance invasion science in the face of rapid global environmental change. First, invasion science should strive to develop a more comprehensive framework for predicting how the behavior, abundance, and interspecific interactions of non-native species vary in relation to conditions in receiving environments and how these factors govern the ecological impacts of invasion. A second priority is to understand the potential synergistic effects of multiple co-occurring stressors— particularly involving climate change—on the establishment and impact of non-native species. Climate adaptation and mitigation strategies will need to consider the possible consequences of promoting non-native species, and appropriate management responses to non-native species will need to be developed. The third priority is to address the taxonomic impediment. The ability to detect and evaluate invasion risks is compromised by a growing deficit in taxonomic expertise, which cannot be adequately compensated by new molecular technologies alone. Management of biosecurity risks will become increasingly challenging unless academia, industry, and governments train and employ new personnel in taxonomy and systematics. Fourth, we recommend that internationally cooperative biosecurity strategies consider the bridgehead effects of global dispersal networks, in which organisms tend to invade new regions from locations where they have already established. Cooperation among countries to eradicate or control species established in bridgehead regions should yield greater benefit than independent attempts by individual countries to exclude these species from arriving and establishing
What’s in the Pool? A Comprehensive Identification of Disinfection By-products and Assessment of Mutagenicity of Chlorinated and Brominated Swimming Pool Water
38 páginas, 2 figuras, 4 tablas.-- PDF con material suplementario.[BACKGROUND]: Swimming pool disinfectants and disinfection by-products (DBPs) have been linked to human health effects, including asthma and bladder cancer, but no studies have provided a comprehensive identification of DBPs in the water and related that to mutagenicity.[OBJECTIVES]: We performed a comprehensive identification of DBPs and disinfectant species in waters from public swimming pools in Barcelona, Catalonia, Spain, that disinfect with either chlorine or bromine, and we determined the mutagenicity of the waters to compare to the analytical results.[METHODS]: We used gas chromatography (GC)/mass spectrometry (MS) to measure THMs in water and GC with electron capture detection (ECD) for air, low and high resolution GC/MS to comprehensively identify DBPs, photometry to measure disinfectant species (free chlorine, monochloroamine, dichloramine, and trichloramine) in the waters, and an ion chromatography method to measure trichloramine in air. We assessed mutagenicity in the Salmonella mutagenicity assay.[RESULTS]: We identified more than 100 DBPs, including many nitrogen-containing DBPs that were likely formed from nitrogen-containing precursors from human inputs, such as urine, sweat, and skin cells. Many DBPs were new and have not been reported previously in either swimming pool or drinking waters. Bromoform levels were greater in the brominated vs. chlorinated pool waters, but many brominated DBPs were also identified in the chlorinated waters. The pool waters were mutagenic at levels similar to that of drinking water (~1200 revertants/L-eq in strain TA100 –S9 mix).[CONCLUSIONS]: This study identified many new DBPs not identified previously in swimming pool or drinking water and found that swimming pool waters are as mutagenic as typical drinking waters.This research was supported by EPA’s intramural research
program and the Spanish grants SAF2005-07643-C03-01 (Plan Nacional) and CP06/00341
(Fondo de Investigación Sanitaria). CMV and LFR have, respectively, a contract and a
predoctoral fellowship by the Instituto de Salud Carlos III (CP06/00341, FI06/00651). CL
acknowledges a grant from the Agreement between Santander-Central Hispano and CSIC.Peer reviewe
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