To what extent can agriculture be reshaped to address healthy and sustainable diets by boosting pulse production locally?

The consumption of unhealthy and environmentally unsustainable diets is a major societal challenge because of its impacts on human health and the environment. The adoption of micronutrient-rich diets with relatively low pressure on natural resources is an important development target at the intersection between sustainability and public health goals. Pulses are known for being rich in proteins and micronutrients, while having several environmental benefits. It is still unclear to what extent improving the nutritional content of diets by boosting pulse consumption can address health concerns associated with micronutrient deficiency without exacerbating environmental impacts. Here we evaluate to what extent environmentally sustainable, healthy diets can be achieved by increasing pulse production, while reducing the areas cultivated with cereals. To that end, taking as case studies two low/middle income countries (i.e., Pakistan and Nigeria) with high prevalence of nutritional diseases among children and women, we study different crop replacement scenarios that can boost pulse supply and comply with the micronutrient requirements suggested by the EAT-Lancet Commission. We find that in Nigeria the recommended pulse intake requirement can be met by increasing the domestic production of pulses, while saving 27% of water consumption. Conversely, in Pakistan the recommended levels of pulse intake are barely met because of the limited area suitable for pulse production. Overall, the current low yields of pulses and the limited availability of land suitable for pulse production are the main constraints to their supply in these two countries

The first absolute gravity and height reference network in Sicily

The purpose of this work is to provide the methodological and instrumental framework for the establishment of a new absolute gravity and height reference network in Sicily. The aim of the network is to contribute to the new reference systems in the Italian area, useful for the scientific and technological activities related to the gravity field and to the proper definition of a modern height system in this region. The network is composed of 5 stations, evenly distributed to form a large mesh, which roughly covers the entire Sicily. Since four of the five selected stations were measured also in the 1990s, it was also possible to evaluate whether long-term gravity changes occurred at these sites (basic requirement for a reference network) and check the long-term ground deformation patterns, using data from the closest GPS/GNSS stations. The observed gravity changes over a time interval of about 30 years at the absolute stations and in the surrounding areas, confirm the long-term stability of the selected areas/sites

Altered gut microbiota and endocannabinoid system tone in vitamin D deficiency-mediated chronic pain

Abstract Recent evidence points to the gut microbiota as a regulator of brain and behavior, although it remains to be determined if gut bacteria play a role in chronic pain. The endocannabinoid system is implicated in inflammation and chronic pain processing at both the gut and central nervous system (CNS) levels. In the present study, we used low Vitamin D dietary intake in mice and evaluated possible changes in gut microbiota, pain processing and endocannabinoid system signaling. Vitamin D deficiency induced a lower microbial diversity characterized by an increase in Firmicutes and a decrease in Verrucomicrobia and Bacteroidetes. Concurrently, vitamin D deficient mice showed tactile allodynia associated with neuronal hyperexcitability and alterations of endocannabinoid system members (endogenous mediators and their receptors) at the spinal cord level. Changes in endocannabinoid (anandamide and 2-arachidonoylglycerol) levels were also observed in the duodenum and colon. Remarkably, the anti-inflammatory anandamide congener, palmitoylethanolamide, counteracted both the pain behaviour and spinal biochemical changes in vitamin D deficient mice, whilst increasing the levels of Akkermansia, Eubacterium and Enterobacteriaceae, as compared with vehicle-treated mice. Finally, induction of spared nerve injury in normal or vitamin D deficient mice was not accompanied by changes in gut microbiota composition. Our data suggest the existence of a link between Vitamin D deficiency – with related changes in gut bacterial composition – and altered nociception, possibly via molecular mechanisms involving the endocannabinoid and related mediator signaling systems

IRT analyses of the Swedish Dark Triad Dirty Dozen

Background: The Dark Triad (i.e., Machiavellianism, narcissism, and psychopathy) can be captured quickly with 12 items using the Dark Triad Dirty Dozen (Jonason and Webster, 2010). Previous Item Response Theory (IRT) analyses of the original English Dark Triad Dirty Dozen have shown that all three subscales adequately tap into the dark domains of personality. The aim of the present study was to analyze the Swedish version of the Method: 570 individuals (nmales = 326, nfemales = 242, and 2 unreported), including university students and white-collar workers with an age range between 19 and 65 years, responded to the Swedish version of the Dark Triad Dirty Dozen (Garcia et al., 2017a,b).Results: Contrary to previous research, we found that the narcissism scale provided most information, followed by psychopathy, and finally Machiavellianism. Moreover, the psychopathy scale required a higher level of the latent trait for endorsement of its items than the narcissism and Machiavellianism scales. Overall, all items provided reasonable amounts of information and are thus effective for discriminating between individuals. The mean item discriminations (alphas) were 1.92 for Machiavellianism, 2.31 for narcissism, and 1.99 for psychopathy.Conclusion: This is the first study to provide IRT analyses of the Swedish version of the Dark Triad Dirty Dozen. Our findings add to a growing literature on the Dark Triad Dirty Dozen scale in different cultures and highlight psychometric characteristics, which can be used for comparative studies. Items tapping into psychopathy showed higher thresholds for endorsement than the other two scales. Importantly, the narcissism scale seems to provide more information about a lack of narcissism, perhaps mirroring cultural conditions.</p

Severe heart failure and intracardiac thrombosis: going beyond the appearance for diagnosis and treatments

We describe the case of a 45-year-old female affected by asthma and nasal polyposis who presented to the emergency department because of worsening dyspnea and paresthesia of the left lower limb. Comprehensive instrumental examinations revealed the presence of severe left ventricle dysfunction, intra-cardiac thrombus, deep vein thrombosis, pulmonary embolism, lung infiltrates, polyserositis, and neurological involvements. Finally, the patient was diagnosed with eosinophilic granulomatosis with polyangiitis (EGPA), formerly Churg-Strauss syndrome, a rare vasculitis of small-medium blood vessels with several organ involvements. Treatment with anticoagulants, corticosteroids, and cyclophosphamide led to a significant clinical improvement. However, a subcutaneous cardiac defibrillator was implanted because of the persistence of severe left ventricular dysfunction and the high cardiovascular risk phenotype. Indeed, several cardiac manifestations may occur in EGPA, particularly in patients with anti-neutrophil cytoplasmic antibody-negative disease. Therefore, clinicians should have high clinical suspicion because cardiac involvement in EGPA results in a poor prognosis if not diagnosed and adequately treated

Development of an innovative microcantilever-based biosensor for 17β-estradiol detection in bovine muscles: preliminary results

17β-estradiol is the most powerful substance with estrogenic effect, commonly used as illegal growth promoter in livestock production. To avoid health risks for consumers, sensitive, reliable and low-cost methods for quantification of extremely low concentrations of such carcinogenic residues in food are needed. Antibody-immobilised microcantilever resonators were proposed as innovative biosensors able to quantify an adsorbed target mass thanks to a shift in resonance frequency. Furthermore, the quantification of masses on the order of few picograms has recently shown to be successfully achievable with very high precision. In this study, we analysed the performance of our microcantilever sensors using extracted samples of bovine muscle from experimental animals, containing variable concentrations of 17β-estradiol (HPLC-MS/MS tested). Preliminary data showed that treated animals are correctly revealed, exhibiting large negative frequency shifts. More experiments, though, are needed to obtain a correct quantification of 17β-estradiol concentration

Evolving trends in the management of acute appendicitis during COVID-19 waves. The ACIE appy II study

Background: In 2020, ACIE Appy study showed that COVID-19 pandemic heavily affected the management of patients with acute appendicitis (AA) worldwide, with an increased rate of non-operative management (NOM) strategies and a trend toward open surgery due to concern of virus transmission by laparoscopy and controversial recommendations on this issue. The aim of this study was to survey again the same group of surgeons to assess if any difference in management attitudes of AA had occurred in the later stages of the outbreak. Methods: From August 15 to September 30, 2021, an online questionnaire was sent to all 709 participants of the ACIE Appy study. The questionnaire included questions on personal protective equipment (PPE), local policies and screening for SARS-CoV-2 infection, NOM, surgical approach and disease presentations in 2021. The results were compared with the results from the previous study. Results: A total of 476 answers were collected (response rate 67.1%). Screening policies were significatively improved with most patients screened regardless of symptoms (89.5% vs. 37.4%) with PCR and antigenic test as the preferred test (74.1% vs. 26.3%). More patients tested positive before surgery and commercial systems were the preferred ones to filter smoke plumes during laparoscopy. Laparoscopic appendicectomy was the first option in the treatment of AA, with a declined use of NOM. Conclusion: Management of AA has improved in the last waves of pandemic. Increased evidence regarding SARS-COV-2 infection along with a timely healthcare systems response has been translated into tailored attitudes and a better care for patients with AA worldwide

Affectively Motivated: Affective Profiles, Motivation, Stress and Energy

Objective: We used the affective profiles model to investigate individual differences in motivation, stress andenergy. The aim was to replicate past findings, but we also focused on matched comparisons within individuals withaffective profiles that are similar in one affective dimension and differ in the other in order to predict changes whenindividuals increase/decrease their experience of positive or negative affect. Methods: A total of 567 participants answered the Positive Affect and Negative Affect Schedule, which was usedfor affective profiling; the Situational Motivation Scale, which measures intrinsic motivation, identified regulation,external regulation, and amotivation; and the Stress-Energy questionnaire. Results: Comparisons between the four different profiles, replicating the past findings, showed that individuals withhigh affective and self-fulfilling profile scored highest in intrinsic motivation, identified regulation, and energy, whilethey scored lowest in external motivation, amotivation, and the self-fulfilling profile, also lowest in stress. Additionally,the matched comparisons showed, for example, that levels of intrinsic motivation increase when negative affect levelsdecrease, and positive affect is kept high when positive affect decreases and negative affect is kept low. Conclusions: One important feature of the affective profiles model is the possibility to compare individuals thatare similar in one affect dimension but differ in the other (Garcia, 2011, 2017). This way of discussing individualdifferences helps to predict what changes could be expected when individuals increase or decrease their experience ofpositive or negative affect. Importantly, the direction of these changes cannot be addressed from cross-sectional dat