Histological evidence of wound healing improvement in rats treated with oral administration of hydroalcoholic extract of Vitis labrusca

Plant extracts rich in phenolic compounds have been demonstrated to accelerate wound healing, but their use by oral route has been poorly studied. The leaves of Vitis labrusca are rich in phenolic acids and flavonoids. The goal of this study was to assess the healing properties of the oral administration of hydroalcoholic extract of V. labrusca leaves (HEVL) in a murine model. HEVL was obtained by Soxhlet and dynamic maceration, and their yield and phenolic acids and flavonoid contents were determined. For the wound healing assay, 8 mm wounds were performed on the back of 48 Wistar rats, assigned into four groups (n=12): CTR (distilled water), HEVL100, HEVL200, and HEVL300 (HEVL at 100, 200, and 300 mg/kg, respectively). On days 7 and 14, wound closure rates were assessed, and the healing wounds were subjected to histological analysis. Soxhlet-obtained extract was selected for the wound healing assay because it provided a higher yield and phenolic acid and flavonoid contents. HEVL significantly reduced leukocytosis in the peripheral blood (p < 0.05), accelerated wound closure (p < 0.05), and improved collagenization (p < 0.05) on day 7, as well as enhanced the epidermal tissue thickness (p < 0.001) and elastic fiber deposition on day 14 (p < 0.01). Furthermore, HEVL promoted an increase in the histological grading of wound healing on both days 7 and 14 (p < 0.01). The doses of 200 and 300 mg/kg provided better results than 100 mg/Kg. Our data provide histological evidence that the oral administration of HEVL improves wound healing in rodents. Therefore, the extract can be a potential oral medicine for healing purposes.Brazilian Council for Research Support (CNPq) and the Foundation of research and technological innovation of Sergipe/Brazil (FAPITEC), and by the Portuguese Science and Technology Foundation (FCT/MCT), European Funds (PRODER/COMPETE)– project UIDB/04469/2020 (strategic fund), co-financed by FEDER, under the Partnership Agreement PT2020info:eu-repo/semantics/publishedVersio

Balanço e análise da sustentabilidade energética na produção orgânica de hortaliças.

Os insumos e serviços utilizados na produção vegetal representam custo energético. Dependendo desses fatores e das produtividades obtidas, a conversão da produção em energia determinará a eficiência energética do sistema. A agricultura orgânica somente atingirá a missão de preservação ambiental se tiver comprovada sustentabilidade energética. Neste trabalho, objetivou-se caracterizar os balanços energéticos dos cultivos orgânicos e analisar sua sustentabilidade, em comparação aos sistemas convencionais. Monitoraram-se campos de produção de dez culturas, de 1991 a 2000 em Domingos Martins-ES. Os dados do sistema convencional foram obtidos pelas médias dos coeficientes técnicos da região. Quantificaram-se os coeficientes técnicos, convertendo suas grandezas físicas em equivalentes energéticos, expressos em kcal. O sistema orgânico gastou 4.571.159 kcal ha-1 e apresentou 12.696.712 kcal ha-1 de energia inserida na colheita, mostrando balanço médio de 2,78. Esse valor foi similar ao obtido no sistema convencional (1,93). As participações dos componentes nos gastos do sistema orgânico foram embalagem (35,8%), composto orgânico (17,2%), irrigação (12,6%), sementes/mudas (12,4%) e mão-de-obra (11,0%), serviços mecânicos (5,0%) e frete (4,5%). Se os custos com embalagens fossem eliminados, os gastos do sistema orgânico seriam reduzidos para 2.930.113 kcal ha-1, aumentando sua eficiência. A maioria dos cultivos orgânicos pode ser considerada sustentável em transformação de energia, com balanços superiores a 1,00 e produção média diária de 80.421 kcal ha-1 por dia, superior à necessidade mínima de 58.064 kcal ha-1

DESENVOLVIMENTO E VALIDAÇÃO DE MÉTODO ANALITICO PARA QUANTIFICAÇÃO DO VORICONAZOL EM CÓRNEA

Introdu&ccedil;&atilde;o e objetivos: O voriconazol (VOR) &eacute; um novo antif&uacute;ngico da classe tiazol de amplo espectro1 e, sua administra&ccedil;&atilde;o t&oacute;pica visa o tratamento de ceratites f&uacute;ngicas. Entretanto, o desenvolvimento de novas formula&ccedil;&otilde;es se faz necess&aacute;rio para aumentar a biodisponibilidade ocular do f&aacute;rmaco. Para este prop&oacute;sito, &eacute; necess&aacute;rio o desenvolvimento de m&eacute;todos de quantifica&ccedil;&atilde;o e extra&ccedil;&atilde;o do f&aacute;rmaco em c&oacute;rnea su&iacute;na. Desta forma, o objetivo foi desenvolver e validar um m&eacute;todo anal&iacute;tico capaz de quantificar o VOR na c&oacute;rnea. Metodologia: VOR foi quantificado por CLAE -UV (255 nm), com fase m&oacute;vel constitu&iacute;da por acetonitrila e &aacute;gua (50:50) e fluxo de 1,0 mL/min. O m&eacute;todo foi validado de acordo com os par&acirc;metros do FDA2. Para a recupera&ccedil;&atilde;o do VOR, as amostras brancas da c&oacute;rnea formam contaminadas com diferentes solu&ccedil;&otilde;es de VOR, secas e extra&iacute;das com metanol, ap&oacute;s agita&ccedil;&atilde;o 60 min em chapa magn&eacute;tica. Resultados e discuss&otilde;es: O m&eacute;todo se mostrou especifico (n&atilde;o apresentando picos interferentes no tempo de reten&ccedil;&atilde;o do VOR), linear (y=19,084x + 2,107, r2= 0,999), preciso e exato (precis&atilde;o e exatid&atilde;o inferior a 5%). A recupera&ccedil;&atilde;o do VOR na c&oacute;rnea foi de 84,79% (&plusmn; 1,34%) para concentra&ccedil;&atilde;o te&oacute;rica de 20 mg/mL e 86,09% (&plusmn; 6,20%) para concentra&ccedil;&atilde;o de 5 mg/mL. Conclus&otilde;es: O m&eacute;todo anal&iacute;tico foi validado e o m&eacute;todo de recupera&ccedil;&atilde;o mostrou-se eficaz para quantifica&ccedil;&atilde;o do VOR na c&oacute;rnea

Development of technologies to support the diagnosis of infectious diseases and cancer to support the primary health care

54/2017). Publisher Copyright: © 2022, The Author(s).Purpose: Primary Health Care (PHC) is the coordinator of health care in Brazil and needs to be strengthened in the diagnostic field to increase health care quality. Aiming to improve the diagnostic tools currently available in PHC, this work describes the process of development and validation of two point-of-care biomedical devices for screening patients with syphilis or different kinds of cancer. Methods: The development of these devices followed nine stages of action based on the requirements established by the Ministry of Health. During development, both systems followed the stages of circuit planning, software simulation to verify the components used, cost assessment for the acquisition of features, simulation in contact matrix, development of the embedded system, and planning of the printed circuit board and storage box. Results: Both devices underwent preliminary functionality tests to assess their quality. The performance tests applied on the device to diagnose syphilis performed 8,733,194 requests, with a flow of 2426 requests/second, reaching the desired parameters of robustness, integrity, durability, and stability. In addition, functioning tests on the cancer-screening device indicated the ability to detect standard fluorescence in a minimal (150 uL) sample volume. Conclusions: Together, the methodology used for developing the devices resulted in promising equipment to improve the diagnosis and meet the requirements for executing technologies for testing and triaging patients in PHC.publishersversionpublishe

INTOXICAÇÃO POR PTERIDIUM ARACHNOIDEUM NO OESTE DE SANTA CATARINA - ESTUDO RETROSPECTIVO (2013-2017)

Samambaia (Pteridium arachnoideum) é uma das plantas tóxicas mais importantes na criação de bovinos de todo o Brasil. O objetivo desse trabalho foi descrever os diagnósticos de intoxicação por samambaia, em bovinos na região oeste de Santa Catarina, realizados pelo Laboratório de Patologia Veterinária (LPV) do IFC – Campus Concórdia, no período entre 2013 e 2017. No período, foram realizadas 641 necropsias de bovinos, sendo que 22 dessas tiveram o diagnóstico de intoxicação por samambaia, totalizando 3,4% da casuística total de necropsias em bovinos. Foram 13 casos de síndrome hemorrágica aguda, seis casos de carcinomas de células escamosas na base da língua, esôfago ou rúmen, e três casos de hematúria enzoótica bovina. Apesar dos inúmeros trabalhos científicos a respeito da toxicidade do P. arachnoideum, o número de diagnósticos dessa intoxicação é alto na região, e a planta ainda é facilmente encontrada. A melhor forma de diminuir as intoxicações é o adequado manejo das pastagens e a correta adubação do solo, evitando com que a planta esteja disponível ao consumo dos animais, porém essa informação precisa ser difundida aos produtores rurais.  Palavras-chave: diagnóstico, patologia, samambaia, hematúria, carcinoma.

The MHC Gene Region of Murine Hosts Influences the Differential Tissue Tropism of Infecting Trypanosoma cruzi Strains

We have previously demonstrated that both parasite genetic variability and host genetic background were important in determining the differential tissue distribution of the Col1.7G2 and JG T. cruzi monoclonal strains after artificial infections in mice. We observed that the JG strain was most prevalent in hearts of mouse lineages with the MHC haplotype H-2d (BALB/c and DBA2), while Col1.7G2 was predominant in hearts from C57BL/6 mice, which have the H-2b haplotype. To assess whether the MHC gene region indeed influenced tissue tropism of T. cruzi, we used the same two parasite strains to infect C57BL/6 (H-2b) and C57BLKS/J (H-2d) mice; the latter strain results from the introgression of DBA2 MHC region into the C57BL/6 background. We also performed ex vivo infections of cardiac explants from four congenic mice lineages with the H-2b and H-2d haplotypes arranged in two different genetic backgrounds: C57BLKS/J (H-2d) versus C57BL/6 (H-2b) and BALB/c (H-2d) versus BALB/B10-H2b (H-2b). In agreement with our former observations, Col1.7G2 was predominant in hearts from C57BL/6 mice (H-2b), but we observed a clear predominance of the JG strain in hearts from C57BLKS/J animals (H-2d). In the ex vivo experiments Col1.7G2 also prevailed in explants from H-2b animals while no predominance of any of the strains was observed in H-2d mice explants, regardless of the genetic background. These observations clearly demonstrate that the MHC region influences the differential tissue distribution pattern of infecting T. cruzi strains, which by its turn may be in a human infection the determinant for the clinical forms of the Chagas disease

The Brazilian Developments on the Regional Atmospheric Modeling System (BRAMS 5.2): An Integrated Environmental Model Tuned for Tropical Areas

We present a new version of the Brazilian developments on the Regional Atmospheric Modeling System where different previous versions for weather, chemistry and carbon cycle were unified in a single integrated software system. The new version also has a new set of state-of-the-art physical parameterizations and greater computational parallel and memory usage efficiency. Together with the description of the main features are examples of the quality of the transport scheme for scalars, radiative fluxes on surface and model simulation of rainfall systems over South America in different spatial resolutions using a scale-aware convective parameterization. Besides, the simulation of the diurnal cycle of the convection and carbon dioxide concentration over the Amazon Basin, as well as carbon dioxide fluxes from biogenic processes over a large portion of South America are shown. Atmospheric chemistry examples present model performance in simulating near-surface carbon monoxide and ozone in Amazon Basin and Rio de Janeiro megacity. For tracer transport and dispersion, it is demonstrated the model capabilities to simulate the volcanic ash 3-d redistribution associated with the eruption of a Chilean volcano. Then, the gain of computational efficiency is described with some details. BRAMS has been applied for research and operational forecasting mainly in South America. Model results from the operational weather forecast of BRAMS on 5 km grid spacing in the Center for Weather Forecasting and Climate Studies, INPE/Brazil, since 2013 are used to quantify the model skill of near surface variables and rainfall. The scores show the reliability of BRAMS for the tropical and subtropical areas of South America. Requirements for keeping this modeling system competitive regarding on its functionalities and skills are discussed. At last, we highlight the relevant contribution of this work on the building up of a South American community of model developers