A bioassay-guided fractionation scheme for characterization of new antibacterial compounds from Prosopis cineraria aerial parts

Background and Objectives:Due to the importance of finding of new antibacterial agents, the antibacterial properties of Prosopis cinerariaaerial parts were investigated using a bioassay guided fractionation scheme. Materials and Methods:The organic extract was prepared via maceration in methanol, followed by the fractionation using n-hexane and ethyl acetate. The MICs of fractions were determined against some human pathogenic bacteria using broth micro-dilution assay. The primary characterization and identification of bioactive substance(s) was based on a bio-autograph- ical method using HPTLC and flash chromatography in parallel with agar overlay assays. Finally the exact mass of effective compound(s) was determined by LC-MS. Results:The best antibacterial activities were related to the ethyl acetate fraction. The effective antibacterial compound of the plant were 2 substances with molecular weight of 348 and 184 Dalton that inhibited the growth of assessed Gram positive bacteria with MIC values lower than 125 to 62.5 µg/ml synergistically. Conclusion: Further analysis using nuclear magnetic resonance could reveal the exact structure of these two antibacterial substances. These 2 effective antibacterial compounds could be applied as lead compound for synthesis of new antibacterial agents. © 2016, Tehran University of Medical Science. All Rights reserved

An insight to HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) pathogenesis; evidence from high-throughput data integration and meta-analysis

Background Human T-lymphotropic virus 1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) is a progressive disease of the central nervous system that significantly affected spinal cord, nevertheless, the pathogenesis pathway and reliable biomarkers have not been well determined. This study aimed to employ high throughput meta-analysis to find major genes that are possibly involved in the pathogenesis of HAM/TSP. Results High-throughput statistical analyses identified 832, 49, and 22 differentially expressed genes for normal vs. ACs, normal vs. HAM/TSP, and ACs vs. HAM/TSP groups, respectively. The protein-protein interactions between DEGs were identified in STRING and further network analyses highlighted 24 and 6 hub genes for normal vs. HAM/TSP and ACs vs. HAM/TSP groups, respectively. Moreover, four biologically meaningful modules including 251 genes were identified for normal vs. ACs. Biological network analyses indicated the involvement of hub genes in many vital pathways like JAK-STAT signaling pathway, interferon, Interleukins, and immune pathways in the normal vs. HAM/TSP group and Metabolism of RNA, Viral mRNA Translation, Human T cell leukemia virus 1 infection, and Cell cycle in the normal vs. ACs group. Moreover, three major genes including STAT1, TAP1, and PSMB8 were identified by network analysis. Real-time PCR revealed the meaningful down-regulation of STAT1 in HAM/TSP samples than AC and normal samples (P = 0.01 and P = 0.02, respectively), up-regulation of PSMB8 in HAM/TSP samples than AC and normal samples (P = 0.04 and P = 0.01, respectively), and down-regulation of TAP1 in HAM/TSP samples than those in AC and normal samples (P = 0.008 and P = 0.02, respectively). No significant difference was found among three groups in terms of the percentage of T helper and cytotoxic T lymphocytes (P = 0.55 and P = 0.12). Conclusions High-throughput data integration disclosed novel hub genes involved in important pathways in virus infection and immune systems. The comprehensive studies are needed to improve our knowledge about the pathogenesis pathways and also biomarkers of complex diseases.Peer reviewe

Behavior of tubular X-joints retrofitted with external ring subjected to compressive loading

Tubular structures are made of hollow steel members with circular cross-sections and connecting them is one of the major challenges in their design. So far, some techniques to improve the performance of tubular connections have been proposed. Most of these methods (e.g., internal ring, doubler plate) can only be used for structures during the design, but there are only a few techniques (e.g., external ring, FRP) which can be applied during both fabrication and service. This paper investigates the ultimate strength of circular hollow section (CHS) X-connections stiffened with external ring subjected to axially compressive load. The SOLID186 in ANSYS was used to establish the finite element (FE) models. In these models, both geometric and material non-linearity were considered. Moreover, the welds joining the chord and brace members were modeled. The validation of the FE model with several experimental data indicated that the proposed FE model can accurately predict the static behavior of the ring-stiffened X-joints under compression. In the next step, 117 FE models were created and analyzed to evaluate the effect of the connection geometry and external ring size on the static capacity through a parametric study. Results indicated that the external ring can considerably increase the initial stiffness. Moreover, the ultimate strength of the ring-reinforced X-joints under brace compression can be up to 367% to that of the strength of the corresponding unreinforced joint. Despite these significant differences between the ultimate strength of un-stiffened and ring-stiffened X-connections under compressive load, the investigations on this type of stiffened joints have been limited to only three X-joint tests. Also, no design equation is available to determine the ultimate strength of X-connections stiffened with the external ring. Hence, the parametric study was followed by the nonlinear regression analysis to propose a theoretical equation for the static design of X-connection stiffened with the external ring in compressive load