Ameliorative effects of the flavonoid fustin in a rat model of trinitrobenzensulfonic acid-induced colitis

Trinitrobenzene sulfonic acid (TNBS)-induced colitis is a widely used animal model that mimics the signs and symptoms of inflammatory bowel disease. Fustin is a flavonoid found in Cotinus coggygria. The aim of the present study was to investigate the effect of fustin isolated from Cotinus coggygria heartwood in a rat model of TNBS-induced colitis. In this experiment, 30 male Wistar rats were used, allocated to three groups: Control, TNBS and TNBS+F10. Colitis was induced by rectal application of TNBS. After the induction of colitis, fustin at a dose of 10 mg/kg was administered orally to TNBS+F10 group in the course of 8 days. Severity of colitis, oxidative stress and inflammation were assessed by macroscopic, histopathological, immunohistochemical and biochemical analyses. Rats from TNBS group demonstrated severe colonic damage. Fustin treatment ameliorated most of the macroscopic and some of the histopathological indices of colonic damage, and restored the activity of the endogenous antioxidant superoxide dismutase in tissue homogenate but did not affect the signs of inflammation measured by the activity of alkaline phosphatase in serum and tissue homogenate, as well as the expression of NF-kB in the colon. In conclusion, the ameliorative effects of fustin in the experimental TNBS-induced colitis might be the result of its antioxidant properties.International Conference: Kliment's Days 2023 - 60 years Faculty of Biology 09/11/2023 - 11/11/2023 Sofia, Bulgari

Effect of Aronia melanocarpa fruit juice on glucose tolerance, lipid metabolism, and obesity in a rat model of metabolic syndrome

Abstract Metabolic syndrome (MS) is a serious health condition. The purpose of this study was to investigate the effects of polyphenol-rich Aronia melanocarpa fruit juice (AMFJ) on glucose tolerance, triglyceride levels, and adipose tissue in rats with MS induced by high-fat high-fructose (HFHF) diet. Fifty rats were allocated in 5 groups: control, MS, MS+AMFJ2.5, MS+AMFJ5, and MS+AMFJ10. In the course of 10 weeks, the control group was on a regular rat diet while the other groups received HFHF diet. During the experiment, control and MS groups were treated daily orally with distilled water (10.0 mL kg−1) and the other three groups – with AMFJ at doses of 2.5, 5.0, and 10.0 mL kg−1, respectively. In MS rats, glucose intolerance, hypertriglyceridemia, visceral obesity, and increased adipocyte size were observed. In AMFJ-treated groups, the serum glucose and triglycerides, as well as visceral fat and adipocyte size decreased significantly and did not differ from those of the control group. AMFJ at doses 2.5 and 5.0 mL kg−1 showed an anti-apoptotic activity in adipocytes, while at the dose of 10 mL kg−1 a pro-apoptotic effect was detected. In conclusion, AMFJ could antagonise most of the negative consequences of HFHF diet on carbohydrate and lipid metabolism in a rat MS model

The flavonoid fustin exerts anti-inflammatory effect in a model of carrageenan-induced paw oedema

Carrageenan-induced paw oedema is a widely used model of acute inflammation. The objective of this study was to assess the effect of the flavonoid fustin on carrageenan-induced acute paw inflammation in rats. Thirty male Wistar rats were distributed equally between three groups – control, F10, and F20. In the course of 1 week, animals were treated once daily by intragastric gavage as follows: control group – with distilled water (10 mL kg −1 ) containing 50 μL Tween 80; groups F10 and F20 – with 10 mL kg −1 suspensions containing fustin in two doses (10 mg kg −1 and 20 mg kg −1 , respectively) and 50 μL Tween 80. After the treatment period, carrageenan was injected in the left hind paw and paw oedema was evaluated 0, 30, 60, 120, 180, 240, and 300 min after the injection using a plethysmometer. In the control group, paw oedema increased gradually and peaked at the 180 th minute. Fustin treatment reduced the oedema in all time intervals and the effect was significant on the 30 th and 60 th minute after the injection. The present study indicated that fustin could suppress acute inflammation

Aronia Melanocarpa Fruit Juice Ameliorates the Histopathological Changes in the Myocardium and Coronary Arteries in a Rat Metabolic Syndrome Model

The purpose of this study was to examine the effect of Aronia melanocarpa fruit juice (AMFJ) on the histology of the myocardium and coronary arteries in metabolic syndrome (MS) rats

Hepatoprotective effect of the flavonoid fustin in a rat model of paracetamol-induced acute liver damage

Skip to content IOP Science home Accessibility Help Search Journals Books Publishing Support Login IOP Conference Series: Earth and Environmental Science Purpose-led Publishing, find out more. PAPER • THE FOLLOWING ARTICLE ISOPEN ACCESS Hepatoprotective effect of the flavonoid fustin in a rat model of paracetamol-induced acute liver damage M Salbashian1, N Stefanova2, M Tzaneva2, D Pavlov3, M Novakovic4, V Tesevic5, A Georgieva1, M Eftimov1, M Reyzov1, M Nikolova2Show full author list Published under licence by IOP Publishing Ltd IOP Conference Series: Earth and Environmental Science, Volume 1305, International Conference: Kliment's Days 2023 - 60 years Faculty of Biology 09/11/2023 - 11/11/2023 Sofia, Bulgaria Citation M Salbashian et al 2024 IOP Conf. Ser.: Earth Environ. Sci. 1305 012017 DOI 10.1088/1755-1315/1305/1/012017 DownloadArticle PDF References Download PDF Article metrics 91 Total downloads Share this article Article and author information Abstract Paracetamol (PCM)-induced toxicity is a well-established pharmacological model. Cotinus coggygria is a medicinal plant rich in polyphenols, including the flavonoid fustin. The aim of the present study was to evaluate the effects of the flavonoid fustin isolated from Cotinus coggygria heartwood in a model of PCM-induced liver damage. Male Wistar rats (n=48) were allocated to four groups: Control, PCM, F5+PCM, F10+PCM. The rodents were treated daily orally for nine consecutive days as follows: groups F5+PCM and F10+PCM – with fustin (suspended in a vehicle) at doses of 5 and 10 mg/kg, respectively; groups Control and PCM – with the vehicle. PCM was injected intraperitoneally (1.0 g/kg) on day 7. At the end of the experiment, serum and liver samples were prepared. PCM caused a severe liver damage confirmed by histopathological, immunohistochemical and biochemical indices. Compared to PCM group, in F5+PCM and F10+PCM groups, the hepatic necrosis, steatosis, ballooning degeneration, inflammation and expression of NF-κB were significantly reduced. Fustin treatment resulted in a significant reduction of serum activities of alanine aminotransferase and gamma-glutamyl transferase to levels that did not differ from the control values. The present study demonstrated a hepatoprotective effect of the flavonoid fustin in a rat model of acute paracetamol-induced toxicity.International Conference: Kliment's Days 2023 - 60 years Faculty of Biology 09/11/2023 - 11/11/2023 Sofia, Bulgari