Additional file 5: Figure S2. of Temporal genetic changes in Plasmodium vivax apical membrane antigen 1 over 19 years of transmission in southern Mexico

Population structure of P. vivax based on pvama1I-II. Colors (red, blue and green) represent the resulting populations (K = 3) from Bayesian clustering analysis. A vertical bar represents each individual. (PDF 197 kb

Controlling in the Selected Business Company

Import 04/11/2015Diplomová práce „Controlling ve vybrané společnosti“ se zabývá problematikou controllingu a jeho využitím ve společnosti STEELTEC CZ, S.R.O. V teoretické části jsou vymezeny základní pojmy z oblasti controllingu, jeho cíle, zásady, úkoly, funkce a nástroje. Praktická část je zaměřena na analýzu controllingu ve vybrané společnosti a navržení vhodných metod a postupů pro efektivnější řízení výroby.Thesis „Controlling in selected company“ deals with issues of controlling and its use in the STEELTEC CZ, S.R.O company. In the theoretical part basic concepts from controlling are define, there are also describe its goals, principles, tasks, functions and instruments. The practical part is focus on the analysis of controlling in selected company and suggestion of appropriate methods and processes for more effective production.117 - Katedra účetnictvívýborn

Mutation frequencies.

<p>Mutation frequencies for each of the 30 positions in 8 genes of 5 strains are shown. The isolates with the greatest number of mutations were B₂ and B_10, which presented the most diversified genotype, and the genes with the greatest number of mutations were <i>adk</i>, <i>icd</i> and <i>recA</i>.</p

Biochemical test of <i>Citrobacter freundii</i> isolates.

<p>Biochemical reaction of <i>C. freundii</i> and <i>S. enterica</i> as they are currently defined and the biochemical reactions of the <i>C. freundii</i> isolates from the phenotypic diversification study.</p><p>+_w weak reaction if positive.</p

Pulsed-field gel electrophoresis profiles of l-<i>Ceu</i>I restriction fragments from <i>C. freundii</i> isolates.

<p>a) Pulsed-field gel shows <i>Ceu</i>I profiles: line 1, <i>C. freundii</i> E9750 NCTC; lane 2, FMU108327/P; lane 3, FMU108327/A₁₀ and lane 4; <i>S.</i> Typhimurium LT2 ATCC 700720. The results of Southern blotting analysis appear with capital letters. These indicate the Ceús individual fragments of <i>C. freundii</i> corresponding to those of <i>S</i>. Typhimurium LT2; b) chromosome sizes and molecular weights of individual <i>Ceu</i>I fragments. It also shows the location of every marker gene according to its correspondence in each <i>Ceu</i>I fragment. The <i>Ceu</i>I-C and <i>Ceu</i>I-F fragments of <i>C. freundii</i> were determined by probing the isolated DNA from <i>S.</i> Typhimurium LT2 <i>Ceu</i>I-C and <i>Ceu</i>I-F fragments; c) the gel from panel a were blotted to N+nylon membranes and probed as follows: lanes 1–4 <i>met</i>H (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0074120#pone.0074120.s003" target="_blank">Table S1</a>); lanes 5–8 DNA from <i>C. freundii</i> fragment <i>Ceu</i>I-H and lanes 9–12, <i>met</i>A. <i>Ceu</i>I fragments identified by the probes are indicated inside the images with capital letters.</p

Proportion of ancestry inferred by STRUCTURE.

<p>Results of the STRUCTURE analysis assuming 4 populations, a) every vertical line represents each of the 49 isolates and indicates the proportion of ancestry from the 4 ancestral populations, using the following colors: green for <i>E. coli</i>, blue for <i>Enterobacter</i> spp. and <i>Klebsiella</i> spp., purple for <i>Citrobacter</i> spp. and red for <i>Salmonella</i> spp.; b) It shows the proportion from <i>Citrobacter</i> and <i>Salmonella</i> ancestries in our isolates, as well as their biochemical phenotypes. It is interesting to highlight the A10 and B2 isolates. In spite of the fact that A10 derivative isolate has 60% identity with <i>Citrobacter</i> ssp., it displayed Salmonella's biochemical phenotype which has remained stable until now; in addition we have the B2 derivative isolate which had a genotype with a majority of identity to <i>Salmonella</i> ssp genus and its biochemical phenotype was variable, thus preventing establishing some biochemical identity.</p

Individual and concatenated maximum likelihood trees.

<p>All of the trees were constructed in Mega 5.01 for a) <i>adk</i>, b) <i>recA</i>, c) <i>gyrB</i>, d) <i>icd</i>, e) <i>purA</i>, f) <i>aph</i>, g) <i>gnd</i>, h) <i>mdh</i> and i) concatenated genes. The assigned names of the genus in which these alleles occur are shown. The scale bar represents the number of substitutions per site.</p

Accession numbers of the chromosome sequences used in this study.

<p>Accession numbers of the chromosome sequences used in this study.</p