Enhanced diffusion of Uranium and Thorium linked to crystal plasticity in zircon

The effects of crystal-plasticity on the U-Th-Pb system in zircon is studied by quantitative microstructural and microchemical analysis of a large zircon grain collected from pyroxenite of the Lewisian Complex, Scotland. Electron backscatter diffraction (EBSD) mapping reveals a c.18° variation in crystallographic orientation that comprises both a gradual change in orientation and a series of discrete low-angle (<4°) boundaries. These microstructural data are consistent with crystal-plastic deformation of zircon associated with the formation and migration of dislocations. A heterogeneous pattern of dark cathodoluminescence, with the darkest domains coinciding with low-angle boundaries, mimics the deformation microstructure identified by EBSD. Geochemical data collected using the Sensitive High Resolution Ion MicroProbe (SHRIMP) shows a positive correlation between concentrations of the elements U, Th and Pb (ranging from 20–60 ppm, 30–110 ppm, and 14–36 ppm, respectively) and Th/U ratio (1.13 – 1.8) with the deformation microstructure. The highest measured concentrations and Th/U coincide with low-angle boundaries. This enrichment is interpreted to reflect enhanced bulk diffusion of U and Th due to the formation and migration of high-diffusivity dislocations. (207)Pb/(206)Pb ages for individual analyses show no significant variation across the grain, and define a concordant, combined mean age of 2451 ± 14 Ma. This indicates that the grain was deformed shortly after initial crystallization, most probably during retrograde Inverian metamorphism at amphibolite facies conditions. The elevated Th over U and consistent (207)Pb/(206)Pb ages indicates that deformation most likely occurred in the presence of a late-stage magmatic fluid that drove an increase in the Th/U during deformation. The relative enrichment of Th over U implies that Th/U ratio may not always be a robust indicator of crystallization environment. This study provides the first evidence of deformation-related modification of the U-Th system in zircon and has fundamental implications for the application and interpretation of zircon trace element data

Neoproterozoic reworking of the Palaeoproterozoic Capricorn Orogen of Western Australia and implications for the amalgamation of Rodinia

Argon isotopic data from mica from the southern Capricorn region of Western Australia record complex intra- and inter-grain systematics that reflect modification due to a range of processes. However, 40Ar/39Ar age distributions, though complex, generally show early Neoproterozoic ages in the west, increasing to Mesoproterozoic ages in the east. Palaeoproterozoic ages associated with cooling after the c.1.8 Ga Capricorn Orogen or c.1.6 Ga Mangaroon Orogen are not preserved. These data reflect cooling from a ~300C thermal overprint that took place prior to 960 Ma that is related to the enigmatic Edmunian Orogeny. These data, combined with sediment provenance data from the Early Neoproterozoic Officer Basin and U-Pb age data from the nearby Pinjarra Orogen, indicate that the late Mesoproterozoic - Neoproterozoic Pinjarra and Edmundian events are dynamically linked and reflect tectonic activity on the western margin of the amalgamated West Australian Craton. The temporal framework for this event suggest a link to the evolving Rodinian supercontinent and reflect the oblique collision of either Greater India or Kalahari cratons with the West Australian Craton. These results illustrate that the temporal evolution of poorly preserved orogens can be constrained by low-temperature thermochronology in the adjacent cratons

Variation in XANES in biotite as a function of orientation, crystal composition, and metamorphic history

Microscale analysis of ferrous:ferric iron ratios in silicate minerals has the potential to constrain geological processes but has proved challenging because textural information and spatial resolution are limited with bulk techniques, and in situ methods have limited spatial resolution. Synchrotron methods, such as XANES, have been hampered by the sensitivity of spectra to crystal orientation and matrix effects. In an attempt to break this nexus, biotites from Tanzania were characterized with a combination of optical microscopy, electron microprobe, Mössbauer analysis, electron backscatter diffraction (EBSD) and X-ray absorption near edge structure (XANES) spectroscopy. Pre-edge and edge characteristics of the FeKa absorption feature were compared to orientation information derived by EBSD and ferric iron content derived from Mössbauer analysis. Statistically significant correlations between measured spectral features and optic/crystallographic orientation were observed for individual samples. However, orientation corrections derived from these correlations did not reduce the uncertainty in Fe3+/Fetot. The observations are consistent with matrix- and ordering-dependency of the XANES features, and further work is necessary if a general formulation for orientation corrections is to be devised

Relationship between microstructures and grain-scale trace element distribution in komatiite-hosted magmatic sulphide ores

Komatiite-hosted nickel sulphides from the Yilgarn Craton (Australia) consist of two main sulphide phases: pyrrhotite (Fe7S8) and pentlandite ((Fe,Ni)9S8); two minor sulphide phases: chalcopyrite (CuFeS2) and pyrite (FeS2) and trace arsenides. Samples of massive sulphides from three deposits with diverse deformation and metamorphic histories (the Silver Swan, Perseverance and Flying Fox deposits) have been studied by electron backscatter diffraction and laser ablation inductively coupled plasma mass spectrometry and nano-scale secondary ion mass spectrometry. These ore bodies were selected to investigate the relationship between microstructures and mineral trace element chemistry in three dominant sulphide species in each deposit. In all three samples, pyrrhotite preserves a strong evidence of crystal plasticity relative to both pentlandite and pyrite. The trace element composition of pyrrhotite shows significant variation in specific elements (Pb, Bi and Ag). This variation correlates spatially with intragrain pyrrhotite microstructures, such as low angle and twin boundaries. Minor signatures of crystal plasticity in pyrite and pentlandite occur in the form of rare low angle boundaries (pentlandite) and mild lattice misorientation (pyrite). Trace element compositions of pentlandite and pyrite show no correlation with microstructures.Variations in pyrrhotite are interpreted as a result of intragrain diffusion during the syn- and post-deformation history of the deposit. Intragrain diffusion can occur either due to bulk diffusion, dislocation–impurity pair diffusion, or by “pipe diffusion”, i.e. along fast diffusion pathways at high and low angle, and twin boundaries. This contribution examines three different diffusion models and suggests that dislocation–impurity pair diffusion and pipe diffusion are the most likely processes behind increased trace element concentration along the microstructures in pyrrhotite. The same phenomenon is observed in samples from three different deposits that experienced widely different metamorphic conditions, implying that the final disposition of these elements reflects a post peak-metamorphic stage of the geological history of all three deposits

Preservation of tetherin and CD4 counter-activities in circulating Vpu alleles despite extensive sequence variation within HIV-1 infected individuals.

The HIV-1 Vpu protein is expressed from a bi-cistronic message late in the viral life cycle. It functions during viral assembly to maximise infectious virus release by targeting CD4 for proteosomal degradation and counteracting the antiviral protein tetherin (BST2/CD317). Single genome analysis of vpu repertoires throughout infection in 14 individuals infected with HIV-1 clade B revealed extensive amino acid diversity of the Vpu protein. For the most part, this variation in Vpu increases over the course of infection and is associated with predicted epitopes of the individual's MHC class I haplotype, suggesting CD8+ T cell pressure is the major driver of Vpu sequence diversity within the host. Despite this variability, the Vpu functions of targeting CD4 and counteracting both physical virus restriction and NF-κB activation by tetherin are rigorously maintained throughout HIV-1 infection. Only a minority of circulating alleles bear lesions in either of these activities at any given time, suggesting functional Vpu mutants are heavily selected against even at later stages of infection. Comparison of Vpu proteins defective for one or several functions reveals novel determinants of CD4 downregulation, counteraction of tetherin restriction, and inhibition of NF-κB signalling. These data affirm the importance of Vpu functions for in vivo persistence of HIV-1 within infected individuals, not simply for transmission, and highlight its potential as a target for antiviral therapy

On the Stellar Populations and Evolution of Star-Forming Galaxies at 6.3 < z < 8.6

We study the physical characteristics of galaxies at 6.3 < z < 8.6, selected from deep near-infrared imaging with the Wide Field Camera 3 (WFC3) on board the Hubble Space Telescope. Accounting for the photometric scatter using simulations, galaxies at z ~ 7 have bluer UV colors compared to typical local starburst galaxies at > 4 sigma confidence. Although these colors necessitate young ages (<100 Myr), low or zero dust attenuation, and low metallicities, these are explicable by normal (albeit unreddened) stellar populations, with no evidence for near-zero metallicities and/or top-heavy initial mass functions. The age of the Universe at these redshifts limits the amount of stellar mass in late-type populations, and the WFC3 photometry implies galaxy stellar masses ~ 10^8 - 10^9 Msol for Salpeter initial mass functions to a limiting magnitude of M_1500 ~ -18. The masses of ``characteristic'' (L*) z > 7 galaxies are smaller than those of L* Lyman break galaxies (LBGs) at lower redshifts, and are comparable to less evolved galaxies selected on the basis of their Lyman alpha emission at 3 < z < 6, implying that the 6.3 < z < 8.6 galaxies are the progenitors of more evolved galaxies at lower redshifts. We estimate that Lyman alpha emission is able to contribute to the observed WFC3 colors of galaxies at these redshifts, with an estimated typical line flux of ~ 10^-18 erg s^-1 cm^-2, roughly a factor of four below currently planned surveys. The integrated UV specific luminosity for the detected galaxies at z ~ 7 and z ~ 8 is within factors of a few of that required to reionize the IGM assuming low clumping factors, implying that in order to reionize the Universe galaxies at these redshifts have a high ( ~ 50%) escape fraction of Lyman continuum photons, possibly substantiated by the very blue colors of this population.Comment: Accepted to the Astrophysical Journal; replaced with accepted version. Minor modifications to sample, conclusions are unchange

Biological responses to glyphosate drift from aerial application in non-glyphosate-resistant corn

BACKGROUND: Glyphosate drift from aerial application onto susceptible crops is inevitable, yet the biological responses to glyphosate drift in crops are not well characterized. The objectives of this research were to determine the effects of glyphosate drift from a single aerial application (18.3m swath, 866 g AE ha−1) on corn injury, chlorophyll content, shikimate level, plant height and shoot dry weight in non-glyphosate-resistant (non-GR) corn. RESULTS: One week after application (WAA), corn was killed at 3m from the edge of the spray swath, with injury decreasing to 18% at 35.4m downwind. Chlorophyll content decreased from 78% at 6m to 22% at 15.8m, and it was unaffected beyond 25.6m at 1 WAA. Shikimate accumulation in corn decreased from 349% at 0m to 93% at 15.8m, and shikimate levels were unaffected beyond 25.6m downwind. Plant height and shoot dry weight decreased gradually with increasing distance. At a distance of 35.4m, corn height was reduced by 14% and shoot dry weight by 10% at 3WAA. CONCLUSIONS: Corn injury and other biological responses point to the same conclusion, that is, injury from glyphosate aerial drift is highest at the edge of the spray swath and decreases gradually with distance. The LD50 (the lethal distance that drift must travel to cause a 50% reduction in biological response) ranged from 12 to 26m among the biological parameters when wind speed was 11.2 kmh−1 and using a complement of CP-09 spray nozzles on spray aircraft

Seamless Bead to Microarray Screening: Rapid Identification of the Highest Affinity Protein Ligands from Large Combinatorial Libraries

SummarySeveral approaches have been developed for screening combinatorial libraries or collections of synthetic molecules for agonists or antagonists of protein function, each with its own advantages and limitations. In this report, we describe an experimental platform that seamlessly couples massively parallel bead-based screening of one-bead one-compound combinatorial libraries with microarray-based quantitative comparisons of the binding affinities of the many hits isolated from the bead library. Combined with other technical improvements, this technique allows the rapid identification of the best protein ligands in combinatorial libraries containing millions of compounds without the need for labor-intensive resynthesis of the hits

Phylogeography of the Rufous vanga and the role of bioclimatic transition zones in promoting speciation within Madagascar

Madagascar is known as a biodiversity hotspot, providing an ideal natural laboratory for investigating the processes of avian diversification. Yet, the phylogeography of Madagascar’s avifauna is still largely unexamined. In this study, we evaluated phylogeographic patterns and species limits within the Rufous Vanga, Schetba rufa, a monotypic genus of forest-dwelling birds endemic to the island. Using an integrative taxonomic approach, we synthesized data from over 4000 ultra-conserved element (UCE) loci, mitochondrial DNA, multivariate morphometrics, and ecological niche modeling to uncover two reciprocally monophyletic, geographically circumscribed, and morphologically distinct clades of Schetba. The two lineages are restricted to eastern and western Madagascar, respectively, with distributions broadly consistent with previously described subspecies. Based on their genetic and morphological distinctiveness, the two subspecies merit recognition as separate species. The bioclimatic transition between the humid east and dry west of Madagascar likely promoted population subdivision and drove speciation in Schetba during the Pleistocene. Our study is the first evidence that an East-West bioclimatic transition zone played a role in the speciation of birds within Madagascar