SOMEWHERE AMONGST THE ASHES

ABSTRACT SOMEWHERE AMONGST THE ASHES By Keith Rebec This story collection explores how human beings deal with loss. Whether the loss stems from death, the loss of personal innocence, or the loss of love, the characters within are forced to make decisions that he or she wouldn\u27t make if given the choice. Some of the characters, in an effort to prevent the same or a similar type of loss from reoccurring in their lives, desperately seek ways to avoid the issues altogether, which further complicates their troubles. Others, unbeknownst to their impending loss, must make split second decisions that will change their lives forever. No matter how well the characters prepare for, or even try to avoid, some potential loss, it always catches up with them and affects each in unexpected ways. Drug abuse, death, and poverty in rural settings set the backdrop for these characters and contribute to whether or not they rise above the challenges and become extraordinary or just fall into the never ending state of being lost

Noninvasive glucose sensing by transcutaneous Raman spectroscopy

We present the development of a transcutaneous Raman spectroscopy system and analysis algorithm for noninvasive glucose sensing. The instrument and algorithm were tested in a preclinical study in which a dog model was used. To achieve a robust glucose test system, the blood levels were clamped for periods of up to 45 min. Glucose clamping and rise/fall patterns have been achieved by injecting glucose and insulin into the ear veins of the dog. Venous blood samples were drawn every 5 min and a plasma glucose concentration was obtained and used to maintain the clamps, to build the calibration model, and to evaluate the performance of the system. We evaluated the utility of the simultaneously acquired Raman spectra to be used to determine the plasma glucose values during the 8-h experiment. We obtained prediction errors in the range of ∼1.5−2  mM. These were in-line with a best-case theoretical estimate considering the limitations of the signal-to-noise ratio estimates. As expected, the transition regions of the clamp study produced larger predictive errors than the stable regions. This is related to the divergence of the interstitial fluid (ISF) and plasma glucose values during those periods. Two key contributors to error beside the ISF/plasma difference were photobleaching and detector drift. The study demonstrated the potential of Raman spectroscopy in noninvasive applications and provides areas where the technology can be improved in future studies.National Science Foundation (U.S.) (CAREER Award (No. CBET-1151154))United States. National Aeronautics and Space Administration (Early Career Faculty Grant (No. NNX12AQ44G))Gulf of Mexico Research Initiative (GoMRI-030)Cullen College of Engineerin

Serotonin in the inferior colliculus fluctuates with behavioral state and environmental stimuli

Neuromodulation by serotonin (5-HT) could link behavioral state and environmental events with sensory processing. Within the auditory system, the presence of 5-HT alters the activity of neurons in the inferior colliculus (IC), but the conditions that influence 5-HT neurotransmission in this region of the brain are unknown. We used in vivo voltammetry to measure extracellular 5-HT in the IC of behaving mice to address this issue. Extracellular 5-HT increased with the recovery from anesthesia, suggesting that the neuromodulation of auditory processing is correlated with the level of behavioral arousal. Awake mice were further exposed to auditory (broadband noise), visual (light) or olfactory (2,5-dihydro-2,4,5-trimethylthiazoline, TMT) stimuli, presented with food or confined in a small arena. Only the auditory stimulus or restricted movement increased the concentration of extracellular 5-HT in the IC. Changes occurred within minutes of stimulus onset, with the auditory stimulus increasing extracellular 5-HT by an average of 5% and restricted movement increasing it by an average of 14%. These findings suggest that the neuromodulation of auditory processing by 5-HT is a dynamic process that is dependent on internal state and behavioral conditions

Pathway toward Sustainable Winter Road Maintenance (Case Study)

Life Cycle Assessment (LCA) method was applied to evaluate the environmental impacts of winter road maintenance managed by an innovative road-weather information system and the impacts of vehicles passing the road during the snowstorm event. A case study refers to 10-hour lasting snowstorm event, considering a specific road section and application of a road-weather information management system to help winter road maintenance agency optimizing activities (salt gritting and/or plowing). Reliable information on the timing of the beginning of the snowstorm event affects (1) the activities of winter road maintenance, (2) the mobility of all vehicles passing the road, and (3) the fuel consumption of the vehicles. Since activities are optimized in case of preventive operation of winter road maintenance, less salt is needed overall. The road remains free of snow cover in case of preventive winter road maintenance operation, meaning that passenger cars and trucks pass the road at normal speed, without undesirable acceleration and braking caused by wheels slipping if snow accumulates on the road. Fuel consumption of vehicles passing salted and snow-free road remains unchanged, while fuel consumption increases in case of snow cover. Reduction of environmental burdens in case of such optimized winter road maintenance operation, is shown in this case study. The overall results of the comparative LCA analysis showed that the use of the road-weather information system in road traffic allows for as much as 25% reduction of environmental footprints. In the scenario where the winter service does not use information system the winter service also uses 40% more salt, which is also related with additional environmental impacts

Extracellular ascorbate modulates glutamate dynamics: role of behavioral activation

<p>Abstract</p> <p>Background</p> <p>A physiological increase in extracellular ascorbate (AA), an antioxidant vitamin found throughout the striatum, elevates extracellular glutamate (GLU). To determine the role of behavioral arousal in this interaction, microdialysis was used to measure striatal GLU efflux in rats tested in either a lights-off or lights-on condition while reverse dialysis either maintained the concentration of AA at 250 μM or increased it to 1000 μM to approximate endogenous changes.</p> <p>Results</p> <p>When lights were off, both locomotion and GLU increased regardless of AA dose. In contrast, animals in the lights-on condition were behaviorally inactive, and infusion of 1000, but not 250, μM AA significantly increased extracellular GLU. Interestingly, when ambient light returned to the lights-off group, 1000 μM prolonged the GLU increase relative to the 250 μM group.</p> <p>Conclusion</p> <p>Our results not only support evidence that elevated striatal AA increases extracellular GLU but also indicate that this effect depends on behavioral state and the corresponding level of endogenous GLU release.</p