On the Nature and Importance of Cultural Tightness-Looseness

Cross-cultural research is dominated by the use of values despite their mixed empirical support and their limited theoretical scope. This article expands the dominant paradigm in crosscultural research by developing a theory of cultural tightness-looseness, the strength of social norms and degree of sanctioning within societies, and advancing a multilevel research agenda for future research. Through an exploration of the top-down, bottom-up, and moderating impact that societal tightness-looseness has on individuals and organizations, as well as on variability across levels of analysis, the theory provides a new and complementary perspective to the values approach

Negotiating Relationally: The Dynamics of the Relational Self In Negotiations

Although negotiation research is thriving, it has been criticized as having an arelational bias—emphasizing autonomy, competition, and rationality over interdependence, cooperation, and relationality. In this article, we advance a new model of relationality in negotiation. Drawing on research in social psychology, we describe the construct of relational self-construals (RSC) and present a temporal model of RSC and negotiation. After delineating the conditions through which RSC becomes accessible in negotiation and conditions that inhibit its use, we discuss how RSC affects negotiators\u27 pre-negotiation psychological states, early and later tactics, and negotiation outcomes. We illustrate a number of distinct relational dynamics that can occur based on the dyadic composition of RSC, each of which brings distinct benefits and costs to the negotiation table. Implications for the science and practice of negotiation are discussed

Culture and Egocentric Perceptions of Fairness in Conflict and Negotiation

In this article, the authors advanced a cultural view of judgment biases in conflict and negotiation. The authors predicted that disputants’ self-serving biases of fairness would be more prevalent in individualistic cultures, such as the United States, in which the self is served by focusing on one’s positive attributes to “stand out” and be better than others, yet would be attenuated in collectivistic cultures, such as Japan, where the self is served by focusing on one’s negative characteristics to “blend in” (S. J. Heine, D. R. Lehman, H. R. Markus, & S. Kitayama, 1999). Four studies that used different methodologies (free recall, scenarios, and a laboratory experiment) supported this notion. Implications for the science and practice of negotiation are discussed

miR-16 family induces cell cycle arrest by regulating multiple cell cycle genes

MicroRNAs (miRNAs) are a class of small regulatory RNAs that are thought to be involved in diverse biological processes by regulating gene expression. Numerous miRNAs have been identified in various species, and many more miRNAs remain to be detected. Generally, hundreds of mRNAs have been predicted to be potential targets of one miRNA, so it is a great challenge to identify the genuine miRNA targets. Here, we generated the cell lines depleted of Drosha protein and screened dozens of transcripts (including Cyclin D1) regulated potentially by miRNA-mediated RNA silencing pathway. On the basis of miRNA expressing library, we established a miRNA targets reverse screening method by using luciferase reporter assay. By this method, we found that the expression of Cyclin D1 (CCND1) was regulated by miR-16 family directly, and miR-16 induced G1 arrest in A549 cells partially by CCND1. Furthermore, several other cell cycle genes were revealed to be regulated by miR-16 family, including Cyclin D3 (CCND3), Cyclin E1 (CCNE1) and CDK6. Taken together, our data suggests that miR-16 family triggers an accumulation of cells in G0/G1 by silencing multiple cell cycle genes simultaneously, rather than the individual target

Alterations in MicroRNA Expression Contribute to Fatty Acid–Induced Pancreatic β-Cell Dysfunction

OBJECTIVE—Visceral obesity and elevated plasma free fatty acids are predisposing factors for type 2 diabetes. Chronic exposure to these lipids is detrimental for pancreatic β-cells, resulting in reduced insulin content, defective insulin secretion, and apoptosis. We investigated the involvement in this phenomenon of microRNAs (miRNAs), a class of noncoding RNAs regulating gene expression by sequence-specific inhibition of mRNA translation

Parenting and toddler self‐regulation in low‐income families: What does sleep have to do with it?

Toddlerhood is a sensitive period in the development of self‐regulation, a set of adaptive skills that are fundamental to mental health and partly shaped by parenting. Healthy sleep is known to be critical for self‐regulation; yet, the degree to which child sleep alters interactive child–parent processes remains understudied. This study examines associations between observed parenting and toddler self‐regulation, with toddler sleep as a moderator of this association. Toddlers in low‐income families (N = 171) and their mothers were videotaped during free play and a self‐regulation challenge task; videos were coded for mothers’ behavior and affect (free play) and toddlers’ self‐regulation (challenge task). Mothers reported their child’s nighttime sleep duration via questionnaire. Results revealed significant Sleep × Maternal Negative Affect and Sleep × Maternal Negative Control interactions. Children who did not experience negative parenting had good self‐regulation regardless of their nighttime sleep duration. For children who did experience negative parenting, self‐regulation was intact among those who obtained more nighttime sleep, but significantly poorer among children who were getting less nighttime sleep. Thus, among children who were reported to obtain less nighttime sleep, there were more robust associations between negative parenting and poorer self‐regulation than among toddlers who were reported to obtain more sleep.RESUMENLos primeros años de la niñez son un período sensible en el desarrollo de la auto‐regulación, un grupo de habilidades adaptables que son fundamentales para la salud mental y a las que en parte les da forma la crianza. Es sabido que el dormir bien es esencial para la auto‐regulación y, aun así, el nivel al que el sueño del niño altera los procesos interactivos entre progenitor y niño permanece poco estudiado. Este estudio examina las asociaciones entre la crianza observada y la auto‐regulación del niño pequeño, tomando como moderador de tal asociación el proceso de dormir del niño pequeño. Se grabó en video a niños pequeños de familias de bajos ingresos (N=171) y sus madres durante una sesión de juego libre y una tarea de auto‐regulación que suponía un reto; los videos fueron codificados en cuanto al comportamiento y afecto de las madres (juego libre) y la auto‐regulación de los niños pequeños (tarea que suponía reto). Las madres reportaron acerca del sueño nocturno de sus niños por medio de un cuestionario. Los resultados revelaron interacciones significativas en cuanto al dormir y el negativo afecto materno, así como el dormir y el negativo control materno. Los niños que no experimentaron una crianza negativa tenían una buena auto‐regulación independientemente de la duración de su sueño nocturno. En el caso de los niños que experimentaron una crianza negativa, la auto‐regulación quedó intacta en aquellos que lograban más tiempo nocturno de dormir, pero fue significativamente más pobre en los niños que tenían menos tiempo de sueño nocturno. Por tanto, en el caso de los niños indicados en el reporte con menos tiempo de dormir nocturno, se dieron asociaciones más robustas entre la crianza negativa y una más pobre auto‐regulación que entre los niños pequeños indicados en el reporte con más tiempo de dormir.RÉSUMÉLa petite enfance est une période sensible dans le développement de l’auto‐régulation, un ensemble de compétences qui sont fondamentales pour la santé mentale et en partie formées par le parentage. L’on sait qu’un sommeil sain est critique pour l’auto‐régulation et pourtant la mesure dans laquelle le sommeil de l’enfant altère les processus interactifs enfant‐parent demeure peu étudiée. Cette étude examine les liens entre le parentage observé et l’auto‐régulation du petit enfant, le sommeil de l’enfant ayant un effet modérateur dans ce lien. Des jeunes enfants de familles issues de milieux défavorisés (N=171) et leurs mères ont été filmés durant un jeu libre et un exercice de défi d’auto‐régulation. Les vidéos ont été codées pour le comportement des mères et l’affect (jeu libre) et l’auto‐régulation des jeunes enfants (exercice de défi). Les mères ont fait état de la durée de sommeil nocturne de leur enfant au moyen d’un questionnaire. Les résultats ont révélé que : sommeil significatif x l’affect négatif maternel et le sommeil x négatif maternel contrôle les interactions. Les enfants qui n’avaient pas fait l’expérience d’un parentage négatif avaient une bonne auto‐régulation quelle qu’ait été la durée du sommeil nocturne. Pour les enfants ayant fait l’expérience d’une parentage négatif, l’auto‐régulation était intacte chez ceux ayant plus dormi, mais bien moindre chez les enfants qui avaient moins dormi. Donc, chez les enfants ayant moins de sommeil nocturne les liens bien plus robustes ont été découverts entre le parentage négatif et une moindre auto‐régulation que chez les petits enfants dormant plus durant la nuit.ZUSAMMENFASSUNGDas Kleinkindalter ist ein sensibler Zeitraum für die Entwicklung der Selbstregulation – einer Reihe von Anpassungsfähigkeiten, die für die psychische Gesundheit grundlegend sind und teilweise durch Erziehung geprägt werden. Gesunder Schlaf ist bekanntlich entscheidend für die Selbstregulation, aber das Ausmaß, in dem der Kinderschlaf interaktive Prozesse zwischen Kind und Eltern verändert, ist bisher nur unzureichend erforscht wurden. Diese Studie untersucht Zusammenhänge zwischen beobachtetem Erziehungsverhalten und der Selbstregulation von Kleinkindern, wobei der Schlaf der Kleinkinder als Moderator dieser Assoziation fungiert. Kleinkinder aus einkommensschwachen Familien (N=171) und ihre Mütter wurden während des freien Spiels und einer herausfordernden Aufgabe zur Selbstregulation gefilmt; die Videos wurden für das Verhalten und die Affekte der Mütter (freies Spiel) und die Selbstregulation der Kleinkinder (herausfordernde Aufgabe) kodiert. Die Mütter berichteten per Fragebogen über die nächtliche Schlafdauer ihres Kindes. Die Ergebnisse zeigten signifikante Interaktionen für Schlaf und mütterlichen negativen Affekt sowie für Schlaf und mütterliche negative Kontrollinteraktionen. Kinder, die keine negative Erziehung erlebten, hatten eine gute Selbstregulation, unabhängig von ihrer nächtlichen Schlafdauer. Bei Kindern, die eine negative Erziehung erfuhren, war die Selbstregulation bei denen, die mehr Nachtschlaf erhielten, intakt und bei Kindern, die weniger Nachtschlaf erhielten, jedoch deutlich schlechter. So gab es bei Kindern, von denen berichtet wurde, dass sie weniger Nachtschlaf erhielten, robustere Assoziationen zwischen negativer Erziehung und schlechterer Selbstregulation als bei Kleinkindern, von denen berichtet wurde, dass sie mehr Schlaf erhielten.抄録低収入家庭における子育てと幼児の自己調整力:睡眠が関与するものとは何か？幼児期は、自己調整力、つまりメンタルヘルスの基礎であり、ある程度までは子育てによって形成される、一連の適応スキルの発達が影響を受けやすい時期である。健康的な睡眠は自己調整力には不可欠のものとして知られているが、子どもの睡眠が子どもと親の相互作用の過程をどの程度まで改めるかについては、いまもなお研究課題のままである。本研究は、観察によって得られた子育てと幼児の自己調整力の関連性について幼児の睡眠を仲介として検討することである。低所得家庭 (N=171) で生活している幼児と母親が自由遊びと自己調整のチャレンジタスクに取り組む間中ビデオ録画した。ビデオデータは母親の行動と感情(自由遊び)と幼児の自己調整力(チャレンジタスク)としてコード化された。子どもの夜間の睡眠時間は母親からの質問紙を通して報告された。その結果、睡眠と母親の否定的感情の間、そして睡眠と母親の否定的コントロールの間には著しい相互関連性が認められた。否定的育児を経験していない子どもは、夜間の睡眠時間に関わらず、よい自己調整力を持っていた。否定的育児を経験した子どもでは、自己調整力はより長い夜間睡眠をとっている子どもにおいては保たれていたが、より短い睡眠時間しかとっていない子どもにおいては著しく低かった。このようにより短い睡眠時間しかとっていないと報告された子どもにおいては、より長い睡眠をとっている幼児より、否定的育児とより低い自己調整力の間により確かな関連性が示された。摘要低收入家庭的育兒和幼兒自我調節:與睡眠有什麼關係？幼兒期是自我調節發展的一個敏感時期， 這是一套適應性技能， 是心理健康的基礎， 部分由養育方式塑造。眾所周知， 健康睡眠對於自我調節至關重要， 然而， 兒童睡眠如何改變兒童 ‐ 父母互動仍未得到充分研究。本研究探討觀察到的養育方式與幼兒自我調節的關聯， 及幼兒睡眠作為這種關聯的調節變數。低收入家庭的幼兒 （N = 171） 和母親在自由遊戲和自我調節挑戰任務中被錄像; 視頻被編碼為母親的行為和情感 （自由遊戲） 和幼兒的自我調節 （挑戰任務）。母親通過問卷報告孩子的夜間睡眠時間。結果顯示顯著的睡眠x母體負面情感和睡眠 x 母體負面控制相互作用。沒有經歷負面養育的孩子， 無論夜間睡眠時間長短， 都有良好的自我調節能力。對於那些經歷過負面養育的孩子， 在夜間睡眠較多的人中， 自我調節是完整的， 但在夜間睡眠較少的孩子中， 自我調節顯著較差。因此， 在夜間睡眠較少的兒童中， 負面育兒和較差的自我調節的關聯性強於較多睡眠的幼兒。ملخصالرعاية الوالدية والتنظيم الذاتي للطفل في الأسر ذات الدخل المنخفض: ما علاقة النوم بذلك ؟الطفولة هي فتره حساسة في تطوير التنظيم الذاتي ، والذي يمثل مجموعه من المهارات التكيفيه التي هي أساسيه للصحة النفسية وتتشكل جزئيا عن طريق الأبوه والأمومه. ومن المعروف ان النوم الصحي أمر بالغ الاهميه للتنظيم الذاتي ، ومع ذلك ، فان الدرجة التي يغير بها نوم الطفل في العمليات التفاعلية التي يقوم بها الطفل مع الوالدين لا تزال غير خاضعة للدراسة الكافية. تتناول هذه الدراسة العلاقات بين الرعاية الوالدية الملحوظة والتنظيم الذاتي للطفل الصغير ، حيث نوم الطفل يمثل المتغير الوسيط في هذه العلاقة. اشترك في الدراسة مجموعة من الأطفال الصغار في الأسر ذات الدخل المنخفض (العدد = 171) وأمهاتهم وتم تصويرهم بالفيديو اثناء اللعب الحر مع تكليفهم بمهمة تحدي التنظيم الذاتي ؛ تم ترميز تسجيلات الفيديو لسلوك الأمهات وعاطفتهم في (اللعب الحر) والتنظيم الذاتي للأطفال الصغار في (مهمة التحدي). وأبلغت الأمهات عن مده النوم الليلي لأطفالهن عن طريق الاستبيان. أظهرت النتائج تفاعلات ذات دلالة إحصائية بين النوم والعاطفة السلبية عند الأمهات وبين النوم والسيطرة السلبية للأمهات . الأطفال الذين لا يعانون من الابوه والامومه السلبية كان لديهم قدرات جيدة على التنظيم الذاتي بغض النظر عن مده النوم ليلا. بالنسبة للأطفال الذين يعانون من الابوه والامومه السلبية ، كان التنظيم الذاتي سليما بين أولئك الذين حصلوا علي المزيد من النوم ليلا ، ولكن أضعف بكثير بين الأطفال الذين كانوا يحصلون علي اقل النوم ليلا. وبالتالي ، فانه من بين الأطفال الذين ابلغ عن حصولهم علي قسط اقل من النوم الليلي ، كانت هناك رابطات اقوي بين الرعاية الوالدية السلبية والتنظيم الذاتي الأقل منها بين الصغار الذين أفيد بأنهم يحصلون علي المزيد من النوم.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/150525/1/imhj21783.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/150525/2/imhj21783_am.pd

Prediction of 7-year psychopathology from mother-infant joint attention behaviours: a nested case–control study

<br>Background: To investigate whether later diagnosis of psychiatric disorder can be predicted from analysis of mother-infant joint attention (JA) behaviours in social-communicative interaction at 12 months.</br> <br>Method: Using data from a large contemporary birth cohort, we examined 159 videos of a mother-infant interaction for joint attention behaviour when children were aged one year, sampled from within the Avon Longitudinal Study of Parents and Children (ALSPAC) cohort. Fifty-three of the videos involved infants who were later considered to have a psychiatric disorder at seven years and 106 were same aged controls. Psychopathologies included in the case group were disruptive behaviour disorders, oppositional-conduct disorder, attention-deficit/hyperactivity disorder, pervasive development disorder, anxiety and depressive disorders. Psychiatric diagnoses were obtained using the Development and Wellbeing Assessment when the children were seven years old.</br> <br>Results: None of the three JA behaviours (shared look rate, shared attention rate and shared attention intensity) showed a significant association with the primary outcome of case–control status. Only shared look rate predicted any of the exploratory sub-diagnosis outcomes and was found to be positively associated with later oppositional-conduct disorders (OR [95% CI]: 1.5 [1.0, 2.3]; p = 0.041).</br><br>Conclusions: JA behaviours did not, in general, predict later psychopathology. However, shared look was positively associated with later oppositional-conduct disorders. This suggests that some features of JA may be early markers of later psychopathology. Further investigation will be required to determine whether any JA behaviours can be used to screen for families in need of intervention.</br&gt

Upregulated sirtuin 1 by miRNA-34a is required for smooth muscle cell differentiation from pluripotent stem cells

© 2015 Macmillan Publishers Limited. All rights reserved. microRNA-34a (miR-34a) and sirtuin 1 (SirT1) have been extensively studied in tumour biology and longevityaging, but little is known about their functional roles in smooth muscle cell (SMC) differentiation from pluripotent stem cells. Using well-established SMC differentiation models, we have demonstrated that miR-34a has an important role in SMC differentiation from murine and human embryonic stem cells. Surprisingly, deacetylase sirtuin 1 (SirT1), one of the top predicted targets, was positively regulated by miR-34a during SMC differentiation. Mechanistically, we demonstrated that miR-34a promoted differentiating stem cells' arrest at G0G1 phase and observed a significantly decreased incorporation of miR-34a and SirT1 RNA into Ago2-RISC complex upon SMC differentiation. Importantly, we have identified SirT1 as a transcriptional activator in the regulation of SMC gene programme. Finally, our data showed that SirT1 modulated the enrichment of H3K9 tri-methylation around the SMC gene-promoter regions. Taken together, our data reveal a specific regulatory pathway that miR-34a positively regulates its target gene SirT1 in a cellular context-dependent and sequence-specific manner and suggest a functional role for this pathway in SMC differentiation from stem cells in vitro and in vivo

MicroRNA-296 is enriched in cancer cells and downregulates p21WAF1 mRNA expression via interaction with its 3′ untranslated region

MicroRNAs (miRNAs) are a class of noncoding small RNAs that act as negative regulators of gene expression. To identify miRNAs that may regulate human cell immortalization and carcinogenesis, we performed comparative miRNA array profiling of human normal and SV40-T antigen immortalized cells. We found that miR-296 was upregulated in immortalized cells that also had activation of telomerase. By an independent experiment on genomic analysis of cancer cells we found that chromosome region (20q13.32), where miR-296 is located, was amplified in 28/36 cell lines, and most of these showed enriched miR-296 expression. Overexpression of miR-296 in human cancer cells, with and without telomerase activity, had no effect on their telomerase function. Instead, it suppressed p53 function that is frequently downregulated during human cell immortalization and carcinogenesis. By monitoring the activity of a luciferase reporter connected to p53 and p21WAF1 (p21) untranslated regions (UTRs), we demonstrate that miR-296 interacts with the p21-3′UTR, and the Hu binding site of p21-3′UTR was identified as a potential miR-296 target site. We demonstrate for the first time that miR-296 is frequently upregulated during immortalization of human cells and contributes to carcinogenesis by downregulation of p53-p21WAF1 pathway

MicroRNAs in pulmonary arterial remodeling

Pulmonary arterial remodeling is a presently irreversible pathologic hallmark of pulmonary arterial hypertension (PAH). This complex disease involves pathogenic dysregulation of all cell types within the small pulmonary arteries contributing to vascular remodeling leading to intimal lesions, resulting in elevated pulmonary vascular resistance and right heart dysfunction. Mutations within the bone morphogenetic protein receptor 2 gene, leading to dysregulated proliferation of pulmonary artery smooth muscle cells, have been identified as being responsible for heritable PAH. Indeed, the disease is characterized by excessive cellular proliferation and resistance to apoptosis of smooth muscle and endothelial cells. Significant gene dysregulation at the transcriptional and signaling level has been identified. MicroRNAs are small non-coding RNA molecules that negatively regulate gene expression and have the ability to target numerous genes, therefore potentially controlling a host of gene regulatory and signaling pathways. The major role of miRNAs in pulmonary arterial remodeling is still relatively unknown although research data is emerging apace. Modulation of miRNAs represents a possible therapeutic target for altering the remodeling phenotype in the pulmonary vasculature. This review will focus on the role of miRNAs in regulating smooth muscle and endothelial cell phenotypes and their influence on pulmonary remodeling in the setting of PAH