Evaluation of antimicrobial and antifungal activity of hydroethanolic extracts of in vitro and ex vitro seedlings of Dionaea muscipula J. Ellis

Objective: To assess the antibacterial and antifungal activity of hydroethanolic extracts of Dionaea muscipula J. Ellis against model bacteria and fungi of agricultural importance. Design/Methodology/Approach: In vitro plants of D. muscipula were propagated and acclimatized for three months. The antibacterial activity of the hydroethanolic extracts against Escherichia coli and Bacillus cereus and the antifungal activity against Aspergillus niger, Fusarium oxysporum and Pseudocercospora fijiensis were evaluated. An analysis of variance (ANOVA) was performed to compare the means obtained with a Tukey test (p?0.05). Results: The hydroethanolic extracts of D. muscipula in vitro and acclimatized showed a bacteriostatic activity against the bacteria E. coli (65.20 and 69.78% inhibition) and B. cereus (91.75 and 92.61% inhibition) and an antifungal activity against the fungus P. fijiensis of 7.56 and 14.21% of inhibition, respectively. Study Limitations/Implications: The hydroethanolic extracts of D. muscipula in vitro and acclimatized did not show antifungal activity against A. niger and F. oxysporum. Findings/Conclusions: The hydroethanolic extracts of D. muscipula in vitro and acclimatized showed a bacteriostatic activity against the bacteria E. coli and B. cereus and antifungal activity against the fungus P. fijiensis.Objective: To evaluate the antibacterial and antifungal activity of hydroethanolic extracts of Dionaea muscipula J. Ellis against bacteria models and fungi of agricultural importance. Design/Methodology/Approach: In vitro plants of D. muscipula were propagated and acclimatized for three months. The antibacterial activity of the hydroethanolic extracts against Escherichia coli and Bacillus cereus was evaluated, and the antifungal activity against Aspergillus niger, Fusarium oxysporum and Pseudocercospora fijiensis. Analysis of variance (ANOVA) was carried out to compare the means obtained with Tukey’s test (p≤0.05). Results: The hydroethanolic extracts of in vitro and acclimatized D. muscipula presented bacteriostatic activity against the bacteria E. coli (65.20 and 69.78 % of inhibition) and B. cereus (91.75 and 92.61 % of inhibition), and antifungal activity against the fungus P. fijiensis of 7.56 and 14.21 % of inhibition, respectively. Study Limitations/Implications: The hydroethanolic extracts of in vitro and acclimatized D. muscipula did not show antifungal activity against A. niger and F. oxysporum. Findings/Conclusions: The hydroethanolic extracts of in vitro and acclimatized D. muscipula presented bacteriostatic activity against bacteria E. coli and B. cereus and antifungal activity against the fungus P. fijiensis

Coupling Flash Vacuum Expansion and Spray Drying to Produce Stable Polyphenolic Extract from Coffee Exocarp

In the present work, the effect of the Flash Vacuum Expansion (FVE) process on the recovery of total phenolic content (TPC) from entire coffee peel was evaluated. The resulting extracts were microencapsulated by spray drying using maltodextrin (MD10) and arabic gum (GA). The FVE increased TPC by 20.97% in the ethanolic extracts. The use of MD10 allowed obtention of smaller, less hygroscopic particles, with better color and flow properties as well as a higher concentration of TPC. Both powders were considered microbiologically stable based on humidity and water activity

Determination of Critical Storage Conditions for Spray-Dried Habanero Pepper (<i>Capsicum chinense</i>) Extracts by Coupling Water Adsorption Isotherms and Glass Transition Temperature

This study aimed to determine storage conditions for microparticles containing habanero pepper extracts with maltodextrin (MD) and a 95:5 w/w mixture with precipitated silica (MDSP) as wall materials. State diagrams (SD) using water adsorption isotherms and glass transition temperatures were created. Monolayer values were 6.17 g (MD) and 6.76 g (MDSP) of water/100 g d.s. Critical water activity values (awC) were 0.49 for MD and 0.41 for MDSP. When stored at aw > awC, both samples underwent physical transformations, with significant color changes (ΔE > 8). Conversely, storage below awC resulted in minimal changes (ΔE < 4), consistent with the SD