In vitro Propagation of a Rare Medicinal Plant Abrus laevigatus E. May

AbstractAn efficient protocol was developed for in vitro propagation of Abrus laevigatus E. May. through induction of Plantlets by indirect organogenesis in nodal segment derived callus tissues. Yellowish green, fragile, nodular callus was induced at the cut surface of the nodal segments cultured on MS fortified with 5.0 mg/1 BAP, 0.2 mg/l KIN and 0.1 mg/1 IBA. The callus differentiated into adventitious shoots when it was sub-cultured on to MS supplemented with 3.0 mg/l BAP + 0.5 mg/1 Kin + 0.5 mg/1 NAA. On an average 6.87 ± 0.26 shoots developed. These micro-shoots were rooted in half-strength MS containing 1.0 mg/1 IBA and the rooted plantlets were transferred to soil after acclimatization. Keywords: Abrus laevigatus E. May, In vitro propagation, Callu

In vitro Multiplication of Important Medicinal Plant Solanum nigrum L.

AbstractMedicinal plants are the most important source of life saving drugs for the majority of the world’s population. The biotechnological methods are important to select, multiply and conserve the critical genotypes of medicinal plants. In vitro regeneration holds tremendous potential for the production of high quality plant-based medicine. During the present investigations, direct multiple shoot formation Solanum nigrum L. in vitro was recorded with BAP 6 mg/lit and IAA 0.5 mg/lit. Maximum shoot emergence was achieved using nodal segment as explant with BAP 6 mg/lit. and IAA 0.5 mg/lit. The well developed shoots were sub cultured on a rooting medium containing IBA. Plants were multiplied in vitro and hardened successfully.Keywords: Solanum nigrum L. Multiplication, Micropropagatio