LHC Prospects on Higgs Boson Searches

The search for the Higgs boson and physics beyond the Standard Model will be the most important tasks of the two multi-purpose experiments ATLAS and CMS, which will be placed inside the LHC 14 TeV proton-proton accelerator. The most recent studies, which have been developed with detailed simulations of the detector geometry and response, has pointed out that the LHC detectors have the possibility to cover the whole Standard Model Higgs boson mass spectrum and most of the MSSM parameter space

Tagging b jets with electrons and muons at CMS

The first results of identification of jets from b quarks with soft-lepton tagging algorithms are presented in this note. Jets are built from the energy deposits in the electromagnetic and hadron calorimeters, with an iterative cone algorithm. Electrons and muons are searched for among the reconstructed charged particle tracks associated to these jets with an angular distance criterion. The muon identification is based on standard muon reconstruction algorithms, exploiting the dedicated muon detectors, while electron identification is based on the extrapolation of charged particle tracks into the calorimeter and a detailed analysis of the calorimeter clusters in the region around the track. Jets from b quarks are identified from the kinematic properties of the leptons relative to the jet and the significance of the three dimensional impact parameter of the lepton with respect to the event vertex. The effect of not incorporating the impact parameter significance, as would be necessary for data collected prior to the installation of the silicon pixel tracking detector, is also studied

New 1,3-Disubstituted Benzo[h]Isoquinoline Cyclen-Based Ligand Platform: Synthesis, Eu3+ Multiphoton Sensitization and Imaging Applications

The development of lanthanide-based luminescent probes with a long emission lifetime has the potential to revolutionize imaging-based diagnostic techniques. By a rational design strategy taking advantage of computational predictions, a novel, water-soluble Eu3+ complex from a cyclen-based ligand bearing 1,3-disubstituted benzo[h]isoquinoline arms was realized. The ligand has been obtained overcoming the lack of reactivity of position 3 of the isoquinoline moiety. Notably, steric hindrance of the heteroaromatic chromophore allowed selective and stoichiometry-controlled insertion of two or three antennas on the cyclen platform without any protection strategy. The complex bears a fourth heptanoic arm for easy conjugation to biomolecules. This new chromophore allowed the sensitization of the metal center either with one or two photons excitation. The suitability as a luminescent bioprobe was validated by imaging BMI1 oncomarker in lung carcinoma cells following an established immunofluorescence approach. The use of a conventional epifluorescence microscope equipped with a linear structured illumination module disclosed a simple and inexpensive way to image confocally Ln-bioprobes by single photon excitation in the 350–400 nm window, where ordinary confocal systems have no excitation sources

Tracking and Alignment with the Silicon Strip Tracker at the CMS Magnet Test Cosmic Challenge

Data were collected with a custom-built sub-structure of the silicon strip tracker, both during the preparation of the Magnet Test Cosmic Challenge and during the challenge itself. These data were used to evaluate performance of track reconstruction and detector alignment algorithms, both with and without magnetic field. The track reconstruction algorithm is described in detail and its performance presented, in terms of its efficiency, resolution and consistency with the results from other sub-detectors. A study of detector alignment is shown, including the use tracker construction information. The effect of alignment on track quality is discussed

A spatial multi-scale fluorescence microscopy toolbox discloses entry checkpoints of SARS-CoV-2 variants in Vero E6 cells

: We exploited a multi-scale microscopy imaging toolbox to address some major issues related to SARS-CoV-2 interactions with host cells. Our approach harnesses both conventional and super-resolution fluorescence microscopy and easily matches the spatial scale of single-virus/cell checkpoints. After its validation through the characterization of infected cells and virus morphology, we leveraged this toolbox to reveal subtle issues related to the entry phase of SARS-CoV-2 variants in Vero E6 cells. Our results show that in Vero E6 cells the B.1.1.7 strain (aka Alpha Variant of Concern) is associated with much faster kinetics of endocytic uptake compared to its ancestor B.1.177. Given the cell-entry scenario dominated by the endosomal "late pathway", the faster internalization of B.1.1.7 could be directly related to the N501Y mutation in the S protein, which is known to strengthen the binding of Spike receptor binding domain with ACE2. Remarkably, we also directly observed the central role of clathrin as a mediator of endocytosis in the late pathway of entry. In keeping with the clathrin-mediated endocytosis, we highlighted the non-raft membrane localization of ACE2. Overall, we believe that our fluorescence microscopy-based approach represents a fertile strategy to investigate the molecular features of SARS-CoV-2 interactions with cells

The 2003 Tracker Inner Barrel Beam Test

Before starting the CMS Silicon Strip Tracker (SST) mass production, where the quality control tests can only be done on single components, an extensive collection of activities aiming at validating the tracker system functionality has been performed. In this framework, a final component prototype of the Inner Barrel part (TIB) of the SST has been assembled and tested in the INFN laboratories and then moved to CERN to check its behaviour in a 25~ns LHC-like particle beam. A set of preproduction single-sided silicon microstrip modules was mounted on a mechanical structure very similar to a sector of the third layer of the TIB and read out using a system functionally identical to the final one. In this note the system setup configuration is fully described and the results of the test, concerning both detector performance and system characteristics, are presented and discussed