Exogenous Trehalose Induces Defenses in Wheat Before and During a Biotic Stress Caused by Powdery Mildew

International audiencePowdery mildew would be one of the most damaging wheat diseases without the extensive use of conventional fungicides. Some of the alternative control strategies currently emerging are based on the use of resistance inducers. The disacharride trehalose (TR) is classically described as an inducer of defenses in plants to abiotic stress. In this work, the elicitor or priming effect of TR was investigated in wheat both before and during a compatible wheat–powdery mildew interaction through molecular, biochemical, and cytological approaches. In noninoculated conditions, TR elicited the expression of genes encoding chitinase (chi, chi1, and chi4 precursor), pathogenesis-related protein 1, as well as oxalate oxidase (oxo). Moreover, lipid metabolism was shown to be altered by TR spraying via the upregulation of lipoxygenase (lox) and lipid-transfer protein (ltp)-encoding gene expression. On the other hand, the protection conferred by TR to wheat against powdery mildew is associated with the induction of two specific defense markers. Indeed, in infectious conditions following TR spraying, upregulations of chi4 precursor and lox gene expression as well as an induction of the LOX activity were observed. These results are also discussed with regard to the impact of TR on the fungal infectious process, which was shown to be stopped at the appressorial germ tube stage. Our findings strongly suggest that TR is a true inducer of wheat defense and resistance, at least toward powdery mildew

High-throughput screening and modes of action of new biocontrol compounds on the wheat-Zymoseptoria tritici pathosystem

International audienc

Criblage à haut-débit et caractérisation du mode d’action de nouveaux produits de biocontrôle sur le pathosystème blé Zymoseptoria tritici.

International audienc

Investing the mode of action of resistance inducers during protection of wheat against powdery mildew

International audienc

La biostimulation du blé : méthodes d’application pour la mise en évidence de l’efficacité de nouvelles molécules.

International audienc

Conception, characterization and behavior towards fungi and bacteria of clay-added α- and β-chitosan-based films

International audienc

Conception, characterization and behavior towards fungi and bacteria of clay-added α- and β-chitosan-based films

International audienc