Effect of gibberellin on seeded Vitis vinifera with special reference to induction of seedlessness

Application of GA (25-50 ppm) to Ana:b-e-Shahi at prebloom stage decreased the bunch weight and berry number but weight of the berries was increased and up to 29.6% seedless berries were induced. GA sprays (25-50 ppm) at 2-3 days after full lbloom increased the bunch and berry weights but few seedless berries were induced.GA application (50-150 ppm) to Bhokri grape at 10-11 days before full bloom formed more than 90% seedless berries but bunch weights and berry number were reduced. WitJh an additional second spray at 7- 8 days after full bloom, berry size was impmved but bunch weights were still reduced. At 4-5 days before full bloom application of GA, up to 41.4 % seedless berries were induced but bunch and berry weights were a,gain reduced. With another spray at postbloom stage bunch and berry weights were brought to the level of control.   In Gros Colman (Pusa) grape, GA application (50-100 ppm) at 5-6 days before full bloom and 2-3 days after full bloom also formed seedless berries but the bunch and berry weights were reduced. IFruit set was also improvP.d at boVh stages of spray. Prebloom GA application (50-150 ppm) to Alamwick grape, produced seedless berries up to 38.29% but ,bunch and berry weights were appreciably decreased. No effect on berry number was noticed.Application of GA (25-75 ppm) at prebloom + posbbloom and postbloom alone decreased the bunch and berry weights and berry number markedly of Bharat Early and Black Hamburg varieties. Toxic effects like shot berry formation and cracking of rachis were noticed. No seedless berries were observed. however, more berries with 1 or 2 seeds were formed at the expense of berries with 3 or 4 seeds. Even GA application at Jow concentrations to these varieties (5-25 ppm) reduced bunch weights and berry number.The differential response of these seeded varieties of Vitis vinifera is attributed to different amounts of growth factors and inhibitors present in them

Differential response of some seeded grape cultivars of Vitis vinifera to gibberellin application

Several seeded cultivar,s of Vitis vinifera varying in seed content and berry size were tested to see their response to GA application. Oultivars with high seed index number and low seed content like Bhokri, Anab-e-Shahi, Gros Colman (Pusa) and Alamwick did not show any toxic effects due to GA application but responded by producing seedless berries and increasing berry weight with prebloom and postbloom application respectively. Alamwick alone, with high seed index number did not respond to GA by increasing the berry weight but it responded by forming seedless berries. Oultivars like Bharat Early, Black Hamburg and Black Muscat with high seed content per berry and low seed index number did not respond to GA by producing seedless berries and increasing berry weight. This differential response of cultivars to exogenous GA is discussed on the basis of physiological differences in the two groups of cultivars

Implications of texture 4 zero lepton mass matrices for U_{e3}

Lepton mass matrices similar to texture 4 zero quark mass matrices, known to be quite successful in explaining the CKM phenomenology, have been considered for finding the mixing matrix element U_{e3} (\equiv s_{13}) respecting the CHOOZ constraint, with s_{12} and \Delta m_{12}^2 constrained by SNP and s_{23} and \Delta m_{23}^2 constrained by ANP. Taking charged lepton mass matrix M_l to be diagonal, we find that the ranges of s_{13} corresponding to different SNP solutions very well include the corresponding values of s_{13} found by Akhmedov et al. by considering neutrino mass matrix M_{\nu} with no texture zeros. Considering M_l and M_{\nu} both to be real and non-diagonal, s_{13} ranges for the four SNP solutions come out to be: \sim 0-0.19 (LMA), 0.038-0.093 (SMA), 0.042-0.095 (LOW), 0.038-0.096 (VO) which remain of the same order when M_l and M_{\nu} are considered to be complex and non-diagonal.Comment: 13 pages, LaTe

CD40 Activation Induces Apoptosis in Cultured Human Hepatocytes via Induction of Cell Surface Fas Ligand Expression and Amplifies Fas-mediated Hepatocyte Death during Allograft Rejection

We propose that a novel mechanism of hepatocyte apoptosis, involving a cooperative interaction between CD40 and Fas, is involved in the hepatocyte loss of chronic liver allograft rejection. We detected increased hepatocyte expression of Fas, Fas ligand (FasL), and CD40 associated with dropout of centrilobular (acinar zone 3) hepatocytes in chronic allograft rejection. Expression of CD40 ligand (CD40L) was also increased but was largely restricted to CD68+ macrophages. A functional role for CD40 and Fas in hepatocyte apoptosis was demonstrated in vitro using primary human hepatocytes and the HepG2 cell line in both of which apoptosis was induced, not only by cross-linking Fas directly but also via CD40 activation. Our data suggest that CD40 activation induces apoptosis via Fas because (a) ligation of CD40 upregulated hepatocyte FasL expression, and (b) apoptosis induced via activation of CD40 was prevented by a neutralizing monoclonal antibody to FasL. Thus, CD40 engagement triggers apoptosis of human hepatocytes and might amplify Fas-dependent hepatocyte apoptosis in chronic rejection and other inflammatory liver diseases in which Fas-mediated apoptosis is involved

Unique Mass Texture for Quarks and Leptons

Texture specific quark mass matrices which are hermitian and hierarchical are examined in detail . In the case of texture 6 zeros matrices, out of sixteen possibilities examined by us, none is able to fit the low energy data (LED), for example, $V_{us} = 0.2196 \pm 0.0023$, $V_{cb} = 0.0395 \pm 0.0017$, $\frac{V_{ub}}{V_{cb}} = 0.08 \pm 0.02$, $V_{td}$ lies in the range $0.004 - 0.013$ (PDG). Similarly none of the 32 texture 5 zeros mass matrices considered is able to reproduce LED. In particular, the latest data from LEP regarding $|V_{ub}|/|V_{cb}|(=0.093\pm0.016)$ rules out all of them. In the texture 4 zeros case, we find that there is a unique texture structure for $U$ and $D$ mass matrices which is able to fit the data.Comment: 12 pages, LaTeX,some changes in the references,minor changes in the text,to appear in Phys Rev D(Rapid communications

Current status of cystic echinococcosis control in the Falkland Islands: has elimination been achieved?

Attempts to control cystic echinococcosis (CE) caused by Echinococcus granulosus in the Falkland Islands have been ongoing for over 50 years. No human cases have been recorded since the 1980s but there is a need to establish if the parasite has been completely eliminated from domestic animals. A study was carried out in 2018/2019 to identify dogs infected with E. granulosus using copro-antigen and copro-polymerase chain reaction (PCR) analysis. In addition, annual slaughter data were analysed to establish infection levels of E. granulosus and 2 other taeniid parasites. Results showed that 4 out of 589 dogs (0.7%) tested positive by copro-antigen analysis. Results from similar surveys carried out in 2010, 2012 and 2014 showed 17 (3%), 0 and 6 (1%) copro-antigen-positive dogs, respectively, with 8 dogs being confirmed by PCR in 2010. Annual abattoir data showed that from 2006 to 2020, 36 sheep were identified with E. granulosus (mean 0.0055%), 14 186 sheep with Taenia hydatigena (mean 2.2%) and 465 with Taenia ovis (mean 0.072%). Prevalences of T. hydatigena and T. ovis showed spontaneous rises in certain years where the infections could also be detected in lambs indicating that viable taeniid eggs were present. Observations of farm management procedures indicated that there were occasions when dogs could get access to infective taeniid material. In conclusion, E. granulosus is still present in sheep and dogs but at low prevalences. The increasing presence of T. hydatigena however, indicates that control measures are defective in some areas and there is potential for a re-emergence of CE

Texture Specific Mass Matrices and CP violating asymmetry in B^o_d({\bar B}^o_d) -> \psi K_S

In the context of texture 4 - zero and texture 5 - zero hierarchical quark mass matrices, CP violating asymmetry in $B^o_d({\bar B}^o_d) \to \psi K_S$ (sin2$\beta$) has been evaluated by considering quark masses at $m_Z$ scale. For a particular viable texture 4 - zero mass matrix the range of sin2\beta is: 0.27 - 0.60 and for the corresponding texture 5 - zero case it is 0.31 - 0.59. Further our calculations reveal a crucial dependence of sin2$\beta$ on light quark masses as well as the phase in this sector.Comment: 10 latex pages, 4 EPS figure

A Novel Universal Primer-Multiplex-PCR Method with Sequencing Gel Electrophoresis Analysis

In this study, a novel universal primer-multiplex-PCR (UP-M-PCR) method adding a universal primer (UP) in the multiplex PCR reaction system was described. A universal adapter was designed in the 5′-end of each specific primer pairs which matched with the specific DNA sequences for each template and also used as the universal primer (UP). PCR products were analyzed on sequencing gel electrophoresis (SGE) which had the advantage of exhibiting extraordinary resolution. This method overcame the disadvantages rooted deeply in conventional multiplex PCR such as complex manipulation, lower sensitivity, self-inhibition and amplification disparity resulting from different primers, and it got a high specificity and had a low detection limit of 0.1 ng for single kind of crops when screening the presence of genetically modified (GM) crops in mixture samples. The novel developed multiplex PCR assay with sequencing gel electrophoresis analysis will be useful in many fields, such as verifying the GM status of a sample irrespective of the crop and GM trait and so on