Luscinia transcriptome reads.

A bzip2 archive containing reads in fastq fromat and sample and species assignment tables in tsv format

Additional file 3: of Patterns of gene flow and selection across multiple species of Acrocephalus warblers: footprints of parallel selection on the Z chromosome

PCR primers and length of PCR products. (DOC 38 kb

Additional file 2: of Patterns of gene flow and selection across multiple species of Acrocephalus warblers: footprints of parallel selection on the Z chromosome

List of analyzed samples including information of sex of each individual and geographic coordinates of the sites, where individual birds were captured. (DOC 103 kb

Data file including information about experiments

A tab-delimited TXT file containing information on individual experiments testing responses of Common Nightingale (Luscinia megarhynchos) males in a contact zone with the Thrush Nightingale (Luscinia luscinia) to playback of three types of stimuli: pure conspecific song (LM), pure heterospecific song (LL), and mixed heterospecific song (mix)

Data file including information about experiments

A XLS spreadsheet containing information on individual experiments testing responses of Common Nightingale (Luscinia megarhynchos) males in a contact zone with the Thrush Nightingale (Luscinia luscinia) to playback of three types of stimuli: pure conspecific song (LM), pure heterospecific song (LL), and mixed heterospecific song (mix)

Asynapsis of individual chromosomes in pachytene oocytes of intersubspecific F1 hybrids and parental controls.

a<p>Each column represents the sum of two or three independent biological replicas. Asynapsis of each Chr was measured in a separate experiment in a separate set of cells. Incidence of asynapsis of a particular Chr is shown for cells with at least one asynapsis. Value in parenthesis is an estimate of overall frequency of asynapsis of a given Chr considering the overall frequency of cells with any asynapsis (Any Chr column). n - number of cells with asynapsis. N - total number of cells examined.</p>b<p>Abbreviations: DX.1sD17F1 – (B6.PWD-Chr X.1s×B6.PWD-Chr 17)F1, DX.1sPF1 – (B6.PWD-ChrX 1s×PWD)F1, PD17F1 – (PWD×B6.PWD-Chr 17)F1.</p

Single QTL mapping of <i>Hstx2</i> on Chr X.

<p>(A) QTL analysis of testes weight in the (B6.PWD-Chr X×B6)F1×PWD cross showed a 1.5-LOD support interval between 34.6 cM to 35.3 cM on Chr X. (B) Distribution of testes weight of males carrying PWD or B6 allele of <i>DXMit87</i> marker with LOD score 30. (C) QTL analysis of sperm count in <i>ductus epididymis</i> shows the same 1.5 LOD support interval as for testes weight. (D) Distribution of sperm count of males carrying PWD or B6 allele of <i>DXMit87</i> marker (Chr X: 66.65 Mb, GRCm38) with LOD score above 20.</p

Super-resolution microscopy of synaptonemal complexes on spreads of (B6.PWD-Chr X.1s×PWD) pachytene spermatocytes.

<p>(A) Detail of a pachytene spermatocyte of a sterile male immunostained by SYCP3 (red) and SYCP1 (green) antibodies. Properly synapsed bivalents (arrows) show two parallel threads of transverse filaments decorated by SYCP1 antibody which form the central region embedded in SYCP3 lateral elements. Unsynapsed chromosomes lack transverse filaments but display some irregular SYCP1 spots (arrowheads). (B) Example of a nonhomologous pairing and/or translocations, and asynapsis in pachynema of (B6.Chr X.1s×PWD)F1 sterile male. Bar 2000 nM.</p

Expression profiling of a cluster of MiRNA genes within the <i>Hstx1/Hstx2</i> critical region.

<p>(A) Log fold-change of expression ratio of PWD versus B6 MiRNA genes in flow-sorted testicular cells. Significant overexpression of Mir465 cluster in PWD germ cells is shown in red. (B) Validation of Mir465 overexpression in PWD primary spermatocytes by qRT PCR. Data normalized to U6 non-coding RNA (C) Log fold-change of expression ratio of MiRNA genes in (PWD×B6)F1 versus (B6×PWD)F1 (abbreviated PB6F1 and B6PF1) 14.5 d old testes. Significant upregulation in red (P<0.05). (D) qRT PCR validation of differences in Mir465 expression. Data normalized to Mir152.</p

doi:10.5061/dryad.2p4t3

Remaining Luscinia samples used in "Genomic islands of differentiation in two songbird species reveal candidate genes for hybrid female sterility" are archived in a previous Dryad submission. Mořkovský L, Pačes J, Rídl J, Reifová R (2015) Data from: Scrimer: designing primers from transcriptome data. Dryad Digital Repository. https://doi.org/10.5061/dryad.2p4t