Genome expression analysis by suppression subtractive hybridization identified overexpression of Humanin, a target gene in gastric cancer chemoresistance

Background: In cancer cells, apoptosis is an important mechanism that influences the outcome of chemotherapy and the development of chemoresistance. To find the genes involved in chemoresistance and the development of gastric cancer, we used the suppression subtractive hybridization method to identify the genes that are overexpressed in gastric cancer tissues compared to normal gastric tissues. Results: In the suppression subtractive hybridization library we constructed, the most highly overexpressed genes were humanin isoforms. Humanin is a recently identified endogenous peptide that has anti-apoptotic activity and has been selected for further study due to its potential role in the chemoresistance of gastric cancer. Upregulation of humanin isoforms was also observed in clinical samples by using quantitative real-time PCR. Among the studied isoforms, humanin isoform 3, with an expression level of 4.166 ± 1.44 fold, was the most overexpressed isoform in GC. Conclusions: The overexpression of humanin in gastric cancer suggests a role for chemoresistance and provides new insight into the biology of gastric cancer. We propose that humanin isoforms are novel targets for combating chemoresistance in gastric cancer. © 2014 Mottaghi-Dastjerdi et al.; licensee BioMed Central Ltd

Identification of novel genes involved in gastric carcinogenesis by suppression subtractive hybridization

Gastric cancer (GC) is one of the most common and life-threatening types of malignancies. Identification of the differentially expressed genes in GC is one of the best approaches for establishing new diagnostic and therapeutic targets. Furthermore, these investigations could advance our knowledge about molecular biology and the carcinogenesis of this cancer. To screen for the overexpressed genes in gastric adenocarcinoma, we performed suppression subtractive hybridization (SSH) on gastric adenocarcinoma tissue and the corresponding normal gastric tissue, and eight genes were found to be overexpressed in the tumor compared with those of the normal tissue. The genes were ribosomal protein L18A, RNase H2 subunit B, SEC13, eukaryotic translation initiation factor 4A1, tetraspanin 8, cytochrome c oxidase subunit 2, NADH dehydrogenase subunit 4, and mitochondrially encoded ATP synthase 6. The common functions among the identified genes include involvement in protein synthesis, involvement in genomic stability maintenance, metastasis, metabolic improvement, cell signaling pathways, and chemoresistance. Our results provide new insights into the molecular biology of GC and drug discovery: each of the identified genes could be further investigated as targets for prognosis evaluation, diagnosis, treatment, evaluation of the response to new anticancer drugs, and determination of the molecular pathogenesis of GC. © The Author(s) 2014

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Heavy metal concentrations in the selected tissues of the Persian sturgeon, Acipenser persicus, from the southern coast of the Caspian Sea

Bioaccumulation of heavy metals including Cd, Pb, Cu and Zn in muscle, gill, liver, kidney and stomach in Acipenser persicus was studied. Fish were collected from the ‘Iranian fishery zone 1’ located between Astara and Kiyahshahr. Samples (n=25) were collected from five stations in the study area during the autumn catch season in 2001. Samples were analyzed by wet digestion with hydrogen peroxide and nitric acid. Analyses were testified using spike method. A sample of bovine liver (CRM 185R) was tested to ascertain reliability of analyses. Digested samples were analyzed using Flame Atomic Absorption Spectrophotometer. The bioaccumulation pattern for Zn in different tissues studied was stomach>liver> kidney> gills>muscle tissue. The mean concentration of Zn in the stomach was 136.6±10.70μg g-1 dry weight. Cu with the maximum concentration of 39.71±8.85μg g-1 dry weight in liver showed a bioaccumulation pattern of liver>kidney>stomach>gills>muscle tissue. The bioaccumulation pattern for Pb was determined as gills>liver> kidney>stomach>muscle tissue. Maximum mean concentrations of Pb 6.87±2.25μg g-1 dry weight belonged to gills. Bioaccumulation pattern for Cd in the different organs studied in A. persicus was kidney>liver>gills>stomach> muscle tissue. Maximum mean concentration of 5.1±0.97μg g-1 dry weight belonged to kidneys and the minimum mean concentration of 0.05±0.007μg g-1 dry weight belonged to muscle tissue. Concentration of the metals in the muscles samples were below the most guidelines for human consumption. Concentrations for heavy metals were lower than the guidelines in some organs and were higher than that in the other organs studied. The observed concentrations do not pose health problems as these organs are not used for human consumption

Decadal-centennial scale monsoon variations in the Arabian Sea during the Early Holocene

An essential prerequisite for the prediction of future climate change due to anthropogenic input is an understanding of the natural processes that control Earth's climate on timescales comparable to human-lifespan. The Early Holocene period was chosen to study the natural climate variability in a warm interval when solar insolation was at its maximum. The monsoonal system of the Tropics is highly sensitive to seasonal variations in solar insolation, and consequently marine sediments from the region are a potential monitor of past climate change. Here we show that during the Early Holocene period rapid

Impact of Demand-Response on the Efficiency and Prices in Real-Time Electricity Markets

International audienceWe study the effect of Demand-Response (DR) in dynamic real-time electricity markets. We use a two-stage market model that takes into account the dynamical aspects of gen-eration, demand, and DR. We study the real-time market prices in two scenarios: in the former, consumers anticipate or delay their flexible loads in reaction to market prices; in the latter, the flexible loads are controlled by an independent aggregator. For both scenarios, we show that, when users are price-takers, any competitive equilibrium is efficient: the players' selfish responses to prices coincide with a socially optimal policy. Moreover, the price process is the same in all scenarios. For the numerical evaluation of the properties of the equilibrium, we develop a solution technique based on the Alternating Direction Method of Multipliers (ADMM) and trajectorial forecasts. The forecasts are computed us-ing wind generation data from the UK. We challenge the assumption that all players have full information. If the as-sumption is verified, then, as expected, the social welfare increases with the amount of DR available, since DR relaxes the ramping constraints of generation. However, if the day-ahead market cannot observe how elastic loads are affected by DR, a large quantity of DR can be detrimental and leads to a decrease in the welfare. Furthermore, the DR operator has an incentive to under-dimension the quantity of avail-able DR. Finally, we compare DR with an actual energy storage system. We find that storage has a faster response-time and thus performs better when only a limited amount is installed. However, storage suffers from charge-discharge in-efficiency: with DR, prices do concentrate on marginal cost (for storage, they do not) and provide a better welfare

Menstrual cycle phase does not predict political conservatism

Recent authors have reported a relationship between women's fertility status, as indexed by menstrual cycle phase, and conservatism in moral, social and political values. We conducted a survey to test for the existence of a relationship between menstrual cycle day and conservatism. 2213 women reporting regular menstrual cycles provided data about their political views. Of these women, 2208 provided information about their cycle date, 1260 provided additional evidence of reliability in self-reported cycle date, and of these, 750 also indicated an absence of hormonal disruptors such as recent hormonal contraception use, breastfeeding or pregnancy. Cycle day was used to estimate day-specific fertility rate (probability of conception); political conservatism was measured via direct self-report and via responses to the "Moral Foundations” questionnaire. We also recorded relationship status, which has been reported to interact with menstrual cycle phase in determining political preferences. We found no evidence of a relationship between estimated cyclical fertility changes and conservatism, and no evidence of an interaction between relationship status and cyclical fertility in determining political attitudes. Our findings were robust to multiple inclusion/exclusion criteria and to different methods of estimating fertility and measuring conservatism. In summary, the relationship between cycle-linked reproductive parameters and conservatism may be weaker or less reliable than previously thought

Millisecond Oscillations in X-Ray Binaries

The first millisecond X-ray variability phenomena from accreting compact objects have recently been discovered with the Rossi X-ray Timing Explorer. Three new phenomena are observed from low-mass X-ray binaries containing low-magnetic-field neutron stars: millisecond pulsations, burst oscillations and kiloHertz quasi-periodic oscillations. Models for these new phenomena involve the neutron star spin, and orbital motion closely around the neutron star and rely explicitly on our understanding of strong gravity and dense matter. I review the observations of these new neutron-star phenomena and possibly related ones in black-hole candidates, and describe the attempts to use them to perform measurements of fundamental physical interest in these systems.Comment: 40 pages, 17 figures, 4 tables - submitted to the Annual Review of Astronomy and Astrophysics; to appear September 200