1,645 research outputs found
Isobutanol production from cellobionic acid in Escherichia coli.
BackgroundLiquid fuels needed for the global transportation industry can be produced from sugars derived from plant-based lignocellulosics. Lignocellulosics contain a range of sugars, only some of which (such as cellulose) have been shown to be utilizable by microorganisms capable of producing biofuels. Cellobionic acid makes up a small but significant portion of lignocellulosic degradation products, and had not previously been investigated as an utilizable substrate. However, aldonic acids such as cellobionic acid are the primary products of a promising new group of lignocellulosic-degrading enzymes, which makes this compound group worthy of study. Cellobionic acid doesn't inhibit cellulose degradation enzymes and so its inclusion would increase lignocellulosic degradation efficiency. Also, its use would increase overall product yield from lignocellulose substrate. For these reasons, cellobionic acid has gained increased attention for cellulosic biofuel production.ResultsThis study describes the discovery that Escherichia coli are naturally able to utilize cellobionic acid as a sole carbon source with efficiency comparable to that of glucose and the construction of an E. coli strain able to produce the drop-in biofuel candidate isobutanol from cellobionic acid. The gene primarily responsible for growth of E. coli on cellobionic acid is ascB, a gene previously thought to be cryptic (expressed only after incurring specific mutations in nearby regulatory genes). In addition to AscB, the ascB knockout strain can be complemented by the cellobionic acid phosphorylase from the fungus Neurospora crassa. An E. coli strain engineered to express the isobutanol production pathway was successfully able to convert cellobionic acid into isobutanol. Furthermore, to demonstrate potential application of this strain in a sequential two-step bioprocessing system, E. coli was grown on hydrolysate (that was degraded by a fungus) and was successfully able to produce isobutanol.ConclusionsThese results demonstrate that cellobionic acid is a viable carbon source for biofuel production. This work suggests that with further optimization, a bacteria-fungus co-culture could be used in decreased-cost biomass-based biofuel production systems
Automorphisms of Partially Commutative Groups II: Combinatorial Subgroups
We define several "standard" subgroups of the automorphism group Aut(G) of a
partially commutative (right-angled Artin) group and use these standard
subgroups to describe decompositions of Aut(G). If C is the commutation graph
of G, we show how Aut(G) decomposes in terms of the connected components of C:
obtaining a particularly clear decomposition theorem in the special case where
C has no isolated vertices.
If C has no vertices of a type we call dominated then we give a semi-direct
decompostion of Aut(G) into a subgroup of locally conjugating automorphisms by
the subgroup stabilising a certain lattice of "admissible subsets" of the
vertices of C. We then characterise those graphs for which Aut(G) is a product
(not necessarily semi-direct) of two such subgroups.Comment: 7 figures, 63 pages. Notation and definitions clarified and typos
corrected. 2 new figures added. Appendix containing details of presentation
and proof of a theorem adde
Neutron emissions in brittle rocks during compression tests: Monotonic vs cyclic loading
Neutron emission measurements, by means of 3He devices and neutron bubble detectors, were performed during two different kinds of compression tests on brittle rocks: (i) under displacement control, and (ii) under cyclic loading. The material used for the tests was Green Luserna Granite, with different specimen sizes and shapes, and consequently with different brittleness numbers. Since the analyzed material contains iron, our conjecture is that piezonuclear reactions involving fission of iron into aluminum, or into magnesium and silicon, should have occurred during compression damage and failure. Some studies have been already conducted on the different forms of energy emitted during the failure of brittle materials. They are based on the signals captured by acoustic emission measurement systems, or on the detection of electromagnetic charge. On the other hand, piezonuclear neutron emissions from very brittle rock specimens in compression have been discovered only very recently. In this paper, the authors analyse this phenomenon from an experimental point of vie
East Asia and the global/transatlantic/Western crisis
This paper introduces the special collection on East Asia and the Global Crisis. After justifying why a focus on East Asia is appropriate, it draws out the main themes that run through the individual contributions. These are the extent to which the region is decoupling from the global economy (or the West), the increasing legitimacy of statist alternatives to neoliberal development strategies, and the impact of crises on the definition of ―region‖ and the functioning of regional institutions and governance mechanisms
COMPARATIVE ANALYSIS OF STRUCTURAL PARAMETERS OBTAINED BY DIFFRACTOMETER AND SCANNING-DENSITOMETER MEASUREMENTS
The structure of cytosine monohydrate has been redetermined with reflexion data obtained from
automatic digital scanning of Weissenberg films. The previous determination with diffractometer data
[McClure & Craven, Acta Cryst. (1973), B29, 1234--1238] converged to an R of 0.037, with
average estimated standard deviations of 0"002 A and 0"15 ° in derived bond lengths and angles. The
photographic data set converged to an R of 0-0672 with e.s.d.'s of 0-004--0-005 A and 0.3-0.4 °. Normal
probability plots showed that the positional (and the derived geometric) parameters were very comparable;
the vibrational parameters were rather less so, some systematic error being apparent. The overall
conclusion from the study is that the two data sets have given essentially the same positional description
of the structure
Faster linearizability checking via -compositionality
Linearizability is a well-established consistency and correctness criterion
for concurrent data types. An important feature of linearizability is Herlihy
and Wing's locality principle, which says that a concurrent system is
linearizable if and only if all of its constituent parts (so-called objects)
are linearizable. This paper presents -compositionality, which generalizes
the idea behind the locality principle to operations on the same concurrent
data type. We implement -compositionality in a novel linearizability
checker. Our experiments with over nine implementations of concurrent sets,
including Intel's TBB library, show that our linearizability checker is one
order of magnitude faster and/or more space efficient than the state-of-the-art
algorithm.Comment: 15 pages, 2 figure
Augmenter of liver regeneration enhances the success rate of fetal pancreas transplantation in rodents
Background. Treatment of fetal pancreas (FP) isografts with insulin- like growth factor-I greatly improves the rate of conversion to euglycemia in diabetic rats. Complete knowledge of other factors that may facilitate the engraftment and function of FP in vivo is still embryonic. Augmenter of liver regeneration (ALR) is a newly described polypeptide growth factor found in weanling rat livers. ALR has trophic effects on regenerating liver. We studied the effects of in situ administration of this agent on FP isografts in rats. Methods. Streptozotocin-diabetic Lewis rats (blood glucose >300 mg/dl) received 16 FP isografts transplanted intramuscularly. ALR was delivered from day 1 through day 14, in doses of 40 or 400 ng/kg/d. Animals were followed for 3 months with serial weights and blood glucose monitoring. These animals were compared with those treated with vehicle alone. Results. Of the group treated with ALR at 40 ng/kg/day for 14 days, 89% (eight of nine) were euglycemic (P=0.0003). Of the group treated with ALR at 400 ng/kg/day for 14 days, 88% (seven of eight) were euglycemic (P=0.0007). Of the group treated with vehicle alone, none of the six were euglycemic. Euglycemia is defined here as glucose<200 mg/dl for 3 days. Pathology of the intramuscular transplant site showed patches of islet tissue embedded in fat. These patches demonstrated insulin immunoreactivity. Conclusions. Diabetes was reversed in a significantly greater proportion of FP + ALR-treated recipients than those animals treated with vehicle alone. Local delivery of growth factors my be used as an adjunct to FP transplantation to improve the rate of success. This in situ model may be useful to further evaluate other soluble factors
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