Mapping human serum induced gene networks as a basis for the creation of biomimetic periosteum for bone repair

The periosteum is a highly vascularised, collagen-rich tissue that plays a crucial role in directing bone repair. This is orchestrated primarily by its resident progenitor cell population. Indeed, preservation of periosteum integrity is critical for bone healing. Cells extracted from the periosteum retain their osteochondrogenic properties and as such are a promising basis for tissue engineering strategies for the repair of bone defects. However, the culture expansion conditions, and the way in which the cells are reintroduced to the defect site are critical aspects of successful translation. Indeed, expansion in human serum and implantation on biomimetic materials has previously been shown to improve in vivo bone formation. As such, this study aimed to develop a protocol to allow for the expansion of human periosteum derived cells (hPDCs) in a biomimetic periosteal-like environment. The expansion conditions were defined through the investigation of the bioactive cues involved in augmenting hPDC proliferative and multipotency characteristics, based on transcriptomic analysis of cells cultured in human serum. Master regulators of transcriptional networks were identified and an optimised periosteal derived-growth factor cocktail (PD-GFC; containing β-Estradiol, FGF2, TNFα, TGFβ, IGF-1 and PDGF-BB) was generated. Expansion of hPDCs in PD-GFC resulted in serum mimicry with regards to the cell morphology, proliferative capacity and chondrogenic differentiation. When incorporated into a 3D collagen-type-1 matrix and cultured in PD-GFC, the hPDCs migrated to the surface that represented the matrix topography of the periosteum cambium layer. Furthermore, gene expression analysis revealed a downregulated Wnt and TGFβ signature and an upregulation of CREB, which may indicate the hPDCs are recreating their progenitor cell signature. This study highlights the first stage in the development of a biomimetic periosteum which may have applications in bone repair

Is There Only One Solution of the Dyson-Schwinger Equation for Quark Propagator in the Case of Non-zero Current Quark Mass

In this letter it is shown on general ground that there exist two qualitatively distinct solutions of the Dyson-Schwinger equation for the quark propagator in the case of non-zero current quark mass. One solution corresponds to the ``Nambu-Goldstone'' phase and the other one corresponds to the ``Wigner'' phase in the chiral limit.Comment: 7 page

Transport of magnetic field by a turbulent flow of liquid sodium

We study the effect of a turbulent flow of liquid sodium generated in the von K\'arm\'an geometry, on the localized field of a magnet placed close to the frontier of the flow. We observe that the field can be transported by the flow on distances larger than its integral length scale. In the most turbulent configurations, the mean value of the field advected at large distance vanishes. However, the rms value of the fluctuations increases linearly with the magnetic Reynolds number. The advected field is strongly intermittent.Comment: 4 pages, 6 figure

Flaring Activity of Sgr A* at 43 and 22 GHz: Evidence for Expanding Hot Plasma

We have carried out Very Large Array (VLA) continuum observations to study the variability of Sgr A* at 43 GHz ($\lambda$=7mm) and 22 GHz ($\lambda$=13mm). A low level of flare activity has been detected with a duration of $\sim$ 2 hours at these frequencies, showing the peak flare emission at 43 GHz leading the 22 GHz peak flare by $\sim20$ to 40 minutes. The overall characteristics of the flare emission are interpreted in terms of the plasmon model of Van der Laan (1966) by considering the ejection and adiabatically expansion of a uniform, spherical plasma blob due to flare activity. The observed peak of the flare emission with a spectral index $\nu^{-\alpha}$ of $\alpha$=1.6 is consistent with the prediction that the peak emission shifts toward lower frequencies in an adiabatically-expanding self-absorbed source. We present the expected synchrotron light curves for an expanding blob as well as the peak frequency emission as a function of the energy spectral index constrained by the available flaring measurements in near-IR, sub-millimeter, millimeter and radio wavelengths. We note that the blob model is consistent with the available measurements, however, we can not rule out the jet of Sgr A*. If expanding material leaves the gravitational potential of Sgr A*, the total mass-loss rate of nonthermal and thermal particles is estimated to be $\le 2\times10^{-8}$ M$_\odot$ yr$^{-1}$. We discuss the implication of the mass-loss rate since this value matches closely with the estimated accretion rate based on polarization measurements.Comment: Revised with new Figures 1 and 2, 17 pages, 4 figures, ApJ (in press

Ground state representations of loop algebras

Let g be a simple Lie algebra, Lg be the loop algebra of g. Fixing a point in S^1 and identifying the real line with the punctured circle, we consider the subalgebra Sg of Lg of rapidly decreasing elements on R. We classify the translation-invariant 2-cocycles on Sg. We show that the ground state representation of Sg is unique for each cocycle. These ground states correspond precisely to the vacuum representations of Lg.Comment: 22 pages, no figur

Calculation of the Chiral Lagrangian Coefficients

We present a systematic way to combine the global color model and the instanton liquid model to calculate the chiral Lagrangian coefficients. Our numerical results are in agreement well with the experimental values.Comment: 7 pages, To appear in Chin.Phys.Lett, Year 200

Discovery of the Optical Counterparts to Four Energetic Fermi Millisecond Pulsars

In the last few years, over 43 millisecond radio pulsars have been discovered by targeted searches of unidentified gamma-ray sources found by the Fermi Gamma-Ray Space Telescope. A large fraction of these millisecond pulsars are in compact binaries with low-mass companions. These systems often show eclipses of the pulsar signal and are commonly known as black widows and redbacks because the pulsar is gradually destroying its companion. In this paper, we report on the optical discovery of four strongly irradiated millisecond pulsar companions. All four sources show modulations of their color and luminosity at the known orbital periods from radio timing. Light curve modelling of our exploratory data shows that the equilibrium temperature reached on the companion's dayside with respect to their nightside is consistent with about 10-30% of the available spin-down energy from the pulsar being reprocessed to increase the companion's dayside temperature. This value compares well with the range observed in other irradiated pulsar binaries and offers insights about the energetics of the pulsar wind and the production of gamma-ray emission. In addition, this provides a simple way of estimating the brightness of irradiated pulsar companions given the pulsar spin-down luminosity. Our analysis also suggests that two of the four new irradiated pulsar companions are only partially filling their Roche lobe. Some of these sources are relatively bright and represent good targets for spectroscopic follow-up. These measurements could enable, among other things, mass determination of the neutron stars in these systems.Comment: 11 pages, 5 tables, 1 figure, 4 online tables. ApJ submitted and referee

Dynamics and thermodynamics of axisymmetric flows: I. Theory

We develop new variational principles to study stability and equilibrium of axisymmetric flows. We show that there is an infinite number of steady state solutions. We show that these steady states maximize a (non-universal) $H$-function. We derive relaxation equations which can be used as numerical algorithm to construct stable stationary solutions of axisymmetric flows. In a second part, we develop a thermodynamical approach to the equilibrium states at some fixed coarse-grained scale. We show that the resulting distribution can be divided in a universal part coming from the conservation of robust invariants and one non-universal determined by the initial conditions through the fragile invariants (for freely evolving systems) or by a prior distribution encoding non-ideal effects such as viscosity, small-scale forcing and dissipation (for forced systems). Finally, we derive a parameterization of inviscid mixing to describe the dynamics of the system at the coarse-grained scale

Increased accuracy of ligand sensing by receptor internalization

Many types of cells can sense external ligand concentrations with cell-surface receptors at extremely high accuracy. Interestingly, ligand-bound receptors are often internalized, a process also known as receptor-mediated endocytosis. While internalization is involved in a vast number of important functions for the life of a cell, it was recently also suggested to increase the accuracy of sensing ligand as the overcounting of the same ligand molecules is reduced. Here we show, by extending simple ligand-receptor models to out-of-equilibrium thermodynamics, that internalization increases the accuracy with which cells can measure ligand concentrations in the external environment. Comparison with experimental rates of real receptors demonstrates that our model has indeed biological significance.Comment: 9 pages, 4 figures, accepted for publication in Physical Review