9 research outputs found
CRISPR-assisted detection of RNA-protein interactions in living cells.
Get PDFWe have developed CRISPR-assisted RNA-protein interaction detection method (CARPID), which leverages CRISPR-CasRx-based RNA targeting and proximity labeling to identify binding proteins of specific long non-coding RNAs (lncRNAs) in the native cellular context. We applied CARPID to the nuclear lncRNA XIST, and it captured a list of known interacting proteins and multiple previously uncharacterized binding proteins. We generalized CARPID to explore binders of the lncRNAs DANCR and MALAT1, revealing the method's wide applicability in identifying RNA-binding proteins
Efficient recovery of the RNA-bound proteome and protein-bound transcriptome using phase separation (OOPS)
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Pooled CRISPR screens with imaging on microraft arrays reveals stress granule-regulatory factors
No full texthnRNP H/F drive RNA G-quadruplex-mediated translation linked to genomic instability and therapy resistance in glioblastoma
No full textComparative transcriptome profiling of virulent and non-virulent Trypanosoma cruzi underlines the role of surface proteins during infection
No full textTASOR is a pseudo-PARP that directs HUSH complex assembly and epigenetic transposon control
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