4,848 research outputs found
Management of rice straw with relay cropping of Chinese milk vetch improved double-rice cropping system production in southern China
Non-additive responses of soil C and N to rice straw and hairy vetch (<i>Vicia villosa roth</i> <i>L</i>) mixtures in a paddy soil
Gravitational Chern-Simons Lagrangians and black hole entropy
We analyze the problem of defining the black hole entropy when Chern-Simons
terms are present in the action. Extending previous works, we define a general
procedure, valid in any odd dimensions both for purely gravitational CS terms
and for mixed gauge-gravitational ones. The final formula is very similar to
Wald's original formula valid for covariant actions, with a significant
modification. Notwithstanding an apparent violation of covariance we argue that
the entropy formula is indeed covariant.Comment: 39 page
Expression of tung tree diacylglycerol acyltransferase 1 in E. coli
<p>Abstract</p> <p>Background</p> <p>Diacylglycerol acyltransferases (DGATs) catalyze the final and rate-limiting step of triacylglycerol (TAG) biosynthesis in eukaryotic organisms. Database search has identified at least 59 DGAT1 sequences from 48 organisms, but the expression of any DGAT1 as a full-length protein in <it>E. coli </it>had not been reported because DGAT1s are integral membrane proteins and difficult to express and purify. The objective of this study was to establish a procedure for expressing full-length DGAT1 in <it>E. coli</it>.</p> <p>Results</p> <p>An expression plasmid containing the open reading frame for tung tree (<it>Vernicia fordii</it>) DGAT1 fused to maltose binding protein and poly-histidine affinity tags was constructed and expressed in <it>E. coli </it>BL21(DE3). Immunoblotting showed that the recombinant DGAT1 (rDGAT1) was expressed, but mostly targeted to the membranes and insoluble fractions. Extensive degradation also occurred. Nonetheless, the fusion protein was partially purified from the soluble fraction by Ni-NTA and amylose resin affinity chromatography. Multiple proteins co-purified with DGAT1 fusion protein. These fractions appeared yellow in color and contained fatty acids. The rDGAT1 was solubilized from the insoluble fraction by seven detergents and urea, with SDS and Triton X-100 being the most effective detergents. The solubilized rDGAT1 was partially purified by Ni-NTA affinity chromatography. PreScission protease digestion confirmed the identity of rDGAT1 and showed extensive precipitation following Ni-NTA affinity purification.</p> <p>Conclusions</p> <p>This study reports the first procedure for expressing full-length DGAT1 from any species using a bacterial expression system. The results suggest that recombinant DGAT1 is degraded extensively from the carboxyl terminus and associated with other proteins, lipids, and membranes.</p
Diazotroph abundance and community structure are reshaped by straw return and mineral fertilizer in rice-rice-green manure rotation
Rare Z-decay into light CP-odd Higgs bosons: a comparative study in different new physics models
Various new physics models predict a light CP-odd Higgs boson (labeled as
) and open up new decay modes for Z-boson, such as ,
and , which could be explored at the GigaZ option of
the ILC. In this work we investigate these rare decays in several new physics
models, namely the type-II two Higgs doublet model (type-II 2HDM), the
lepton-specific two Higgs doublet model (L2HDM), the nearly minimal
supersymetric standard model (nMSSM) and the next-to-minimal supersymmetric
standard model (NMSSM). We find that in the parameter space allowed by current
experiments, the branching ratios can reach for
(), for and for , which
implies that the decays and may be accessible
at the GigaZ option. Moreover, since different models predict different
patterns of the branching ratios, the measurement of these rare decays at the
GigaZ may be utilized to distinguish the models.Comment: Version in JHEP (discussions added, errors corrected
Archaea are the predominant and responsive ammonia oxidizing prokaryotes in a red paddy soil receiving green manures
A path planning approach of 3D manipulators using zenithal gnomic projection and polar piecewise interpolation.
In this paper, the mathematical modeling and trajectory planning of a 3D rotating manipulator composed of a rotating-prismatic joint and multiple rigid links is considered. Possible trajectories of the end effector of the manipulator—following a sequence of 3D target points under the action of 2 external driving torques and an axial force—are modeled using zenithal gnomic projections and polar piecewise interpolants expressed as polynomial Hermite-type functions. Because of the geometry of the manipulator, the time-dependent generalized coordinates are associated with the spherical coordinates named the radial distance related to the manipulator length, and the polar and azimuthal angles describing the left and right and, respectively, up and down motion of the manipulator. The polar trajectories (left and right motion) of the end effector are generated using a inverse geometric transformation applied to the polar piecewise interpolants that approximate the gnomic projective trajectory of the 3D via-points. The gnomic via-points—located on a projective plane situated on the northern hemisphere—are seen from the manipulator base location, which represents the center of rotation of the extensible manipulator. The related azimuthal trajectory (up and down motion) is generated by polar piecewise interpolants on the azimuthal angles. Smoothness of the polygonal trajectory is obtained through the use of piecewise interpolants with continuous derivatives, while the kinematics and dynamics implementation of the model is well suited to computer implementation (easy calculation of kinematics variables) and simulation. To verify the approach and validate the model, a numerical example—implemented in Matlab—is presented, and the results are discussed
Modelling of content-aware indicators for effective determination of shot boundaries in compressed MPEG videos
In this paper, a content-aware approach is proposed to design multiple test conditions for shot cut detection, which are organized into a multiple phase decision tree for abrupt cut detection and a finite state machine for dissolve detection. In comparison with existing approaches, our algorithm is characterized with two categories of content difference indicators and testing. While the first category indicates the content changes that are directly used for shot cut detection, the second category indicates the contexts under which the content change occurs. As a result, indications of frame differences are tested with context awareness to make the detection of shot cuts adaptive to both content and context changes. Evaluations announced by TRECVID 2007 indicate that our proposed algorithm achieved comparable performance to those using machine learning approaches, yet using a simpler feature set and straightforward design strategies. This has validated the effectiveness of modelling of content-aware indicators for decision making, which also provides a good alternative to conventional approaches in this topic
Effect of sandblasting, etching and resin bonding on the flexural strength/bonding of novel glass-ceramics
Dr Brian Schottlander (Davis Schottlander Davis Ltd.
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