Quantification of virus syndrome in chili peppers

One of the most important problems to produce chili crops is the presence of diseases caused by pathogen agents, such as viruses, therefore, there is a substantial necessity to better predict the behavior of the diseases of these crops, determining a more precise quantification of the disease’s syndrome that allows the investigators to evaluate better practices, from handling to the experimental level and will permit producers to take opportunistic corrective action thereby, reducing production loses and increasing the quality of the crop. This review discussed methods that have been used for the quantification of disease in plants, specifically for chili peppers crops, thereby, suggesting a better alternative for the quantification of the disease’ syndromes in regards to this crop. The result of these reflections indicates that most methods used for quantification are based on visual assessments, discarding differences of data between distinctive evaluators. These methods generate subjective results.Key words: Quantification, plant diseases, severity, syndrome, viruses

Use of elicitors as an approach for sustainable agriculture

Plant pathogens are responsible for large declines in agricultural production. Their control is carried out mainly by chemical and frequently proposed biological methods to reduce their environmental impact. On the other hand, plant-pathogen or microbe interactions generate multiple signals within plants activating defense mechanism, some of which can also be induced by elicitors (protective molecules). Elicitor-induced plant signaling serves as a guide to a series of intracellular events that end in activation of transduction cascades and hormonal pathways triggering induced resistance (IR) and consequently activation of plant immunity to environmental stresses. So, it is necessary to understand where and how elicitors act in cellular defense mechanism of crops, to improve protection and management for sustainable crop. Therefore this review focused on main topics that guide induced resistance and therefore activation of plant immune response.Keywords: Elicitors, defense mechanism, Immune response, Induced resistance, MAP

Analogies between geminivirus and oncovirus: Cell cycle regulation

Geminiviruses are a large family of plant viruses whose genome is composed of one or two circular and single strand of DNA. They replicate in the cell nucleus being Rep protein, the only viral protein necessary for their replication process. Geminiviruses as same as animal DNA oncoviruses, like SV40, adenovirus and papillomavirus, use the host replication machinery to replicate their DNA. Consequently, they alter host cell cycle regulation to create a suitable environment for their replication. One of the events involved in this alteration would be the inactivation of the retinoblastoma protein (pRb) that negatively regulates the G1/S transition in cells. The discovery of one homologue of the pRb in plants and the finding that Rep protein of some geminiviruses interacts with human retinoblastoma protein, as well as animal virus oncoproteins, is very interesting. This finding laid the groundwork for subsequent detection of analogies between geminiviruses and animal DNA tumor viruses, especially in their interaction with pRb. Moreover, the finding allowed the determination of how this interaction affects the regulation of the cell cycle in plants and animals. Accumulated knowledge generates new interesting questions and possible implications, and so, in this document, we dare to watch in that direction.Key words: Geminivirus, oncovirus, retinoblastoma protein, cell cycle regulation, endoreduplication

Mathematical modeling tendencies in plant pathology

Nowadays plant diseases represent one of the major threats for crops around the world, because they carry healthy, economical, environmental and social problems. Considering this, it is necessary to have a description of the dynamics of plant disease in order to have sustainable strategies to prevent and diminish the impact of the diseases in crops. Mathematical tools have been employed to create models which give a description of epidemic dynamics; the commonly mathematical tools used are: Diseaseprogress curves, Linked Differential Equation (LDE), Area Under disease Progress Curve (AUDPC) and computer simulation. Nevertheless, there are other tools that have been employed in epidemiology of plant disease like: statistical tools, visual evaluations and pictorial assessment. Each tool has its own advantages and disadvantages. The nature of the problem and the epidemiologist necessities determine the mathematical tool to be used and the variables to be included into the model. This paperpresents review of the tools used in epidemiology of plant disease remarking their advantages and disadvantages and mathematical modeling tendencies in plant pathology

Core-collapse supernova progenitor constraints using the spatial distributions of massive stars in local galaxies

We study the spatial correlations between the H$\alpha$ emission and different types of massive stars in two local galaxies, the Large Magellanic Cloud (LMC) and Messier 33. We compare these to correlations derived for core-collapse supernovae (CCSNe) in the literature to connect CCSNe of different types with the initial masses of their progenitors and to test the validity of progenitor mass estimates which use the pixel statistics method. We obtain samples of evolved massive stars in both galaxies from catalogues with good spatial coverage and/or completeness, and combine them with coordinates of main-sequence stars in the LMC from the SIMBAD database. We calculate the spatial correlation of stars of different classes and spectral types with H$\alpha$ emission. We also investigate the effects of distance, noise and positional errors on the pixel statistics method. A higher correlation with H$\alpha$ emission is found to correspond to a shorter stellar lifespan, and we conclude that the method can be used as an indicator of the ages, and therefore initial masses, of SN progenitors. We find that the spatial distributions of type II-P SNe and red supergiants of appropriate initial mass ($\gtrsim$9 $M_{\odot}$) are consistent with each other. We also find the distributions of type Ic SNe and WN stars with initial masses $\gtrsim$20 $M_{\odot}$ consistent, while supergiants with initial masses around 15 $M_{\odot}$ are a better match for type IIb and II-L SNe. The type Ib distribution corresponds to the same stellar types as type II-P, which suggests an origin in interacting binaries. On the other hand, we find that luminous blue variable stars show a much stronger correlation with H$\alpha$ emission than do type IIn SNe.ERC, STF

Development of efficient recirculation system for Tilapia (Oreochromis niloticus) culture using low cost materials

In the current experiment, a recirculation system was built using low cost materials that are available locally and its performance was tested. The performance evaluation procedure was carried out in an aquaculture system in greenhouse with sex-reversed male Nile tilapia (Oreochromis niloticus) production in Querétaro State, Mexico. The recirculation system had four sections (sediment collector, gravel and sand filters, biofilter and clarification) in order to eliminate the organic matter produced by the fish excretion material and uneaten food, as well as, the nitrogenous compounds undesirable in the water tanks, such as, total ammonia nitrogen (TAN). The monitored variables include: temperature, dissolved oxygen, pH, visibility, TAN, nitrites and nitrates. The obtained data were compared with previous studies to evaluate the achieved state of the system. This research clearly demonstrated that it is feasible to use the proposed configuration in aquaculture systems in areas where water source is limited. Consequently, the obtained results represent an environmental standpoint for the conservation of water use in the aquaculture industry and also constitute an important contribution to the aquaculture and farmers who receive minimal economic support.Key words: Water recirculation, aquaculture, sustainability, low cost, water use efficiency

Homologue expression of a fungal endo-1,4-β-Dxylanase using submerged and solid substrate fermentations

The xyn5 gene, which encodes an endo-β-1,4-xylanase (Xyn5), in Aspergillus niger GS1 was cloned into an expression cassette under the control of constitutive glyceraldhehyde-3-phosphate dehydrogenase gene promoter. The expression system was designed to produce the recombinant enzyme containing a six-histidine peptide fused to the carboxyl end of the protein. The efficiency of Xyn5 production under submerged (SmF) and solid-state (SSF) fermentation was investigated using the homologous cotransformed A. niger AB4.1. A productivity of 17.1 U/(l·h) was estimated for SSF and 3.2 U/(l·h) for SmF calculated at peak value of enzyme titers. Recombinant Xyn5 obtained by SSF on polyurethane fiber, was purified 5.1-fold by anion exchange and immobilized metal affinity chromatography, with 35.7% recovery. The purified recombinant enzyme showed an apparent molecular weight of 30 kDa and optimal activity (522 U/mg protein) at pH 5.5 and 50°C.Key words: Aspergillus niger GS1, xylanolytic activity, solid-state fermentation, homologue expression, polyurethane fiber

Selective Reagent Ion Mass Spectrometric Investigations of the Nitroanilines

This paper presents an investigation of proton and charge transfer reactions to 2-, 3- and 4-nitroanilines (C6H6N2O2) involving the reagent ions H3O+·(H2O)n (n=0, 1 and 2) and O2+, respectively, as a function of reduced electric field (60-240 Td), using Selective Reagent Ion-Time-of-Flight-Mass Spectrometry (SRI-ToF-MS). To aid in the interpretation of the H3O+·(H2O)n experimental data, the proton affinities and gas-phase basicities for the three nitroaniline isomers have been determined using density functional theory. These calculations show that proton transfer from both the H3O+ and H3O+·H2O reagent ions to the nitroanilines will be exoergic and hence efficient, with the reactions proceeding at the collisional rate. For proton transfer from H3O+ to the NO2 sites, the exoergicities are 171 kJ mol-1 (1.8 eV), 147 kJ mol-1 (1.5 eV) and 194 kJ mol-1 (2.0 eV) for 2-, 3- and 4-nitroanilines, respectively. Electron transfer from all three of the nitroanilines is also significantly exothermic by approximately 4 eV. Although a substantial transfer of energy occurs during the ion/molecule reactions, the processes are found to predominantly proceed via non-dissociative pathways over a large reduced electric field range. Only at relatively high reduced electric fields (>180 Td) is dissociative proton and charge transfer observed. Differences in fragment product ions and their intensities provide a means to distinguish the isomers, with proton transfer distinguishing 2-nitroaniline (2-NA) from 3- and 4-NA, and charge transfer distinguishing 4-NA from 2- and 3-NA, thereby providing a means to enhance selectivity using SRI-ToF-MS.(VLID)4826158Version of recor

Behavioral Modernity and the Cultural Transmission of Structured Information: The Semantic Axelrod Model

Cultural transmission models are coming to the fore in explaining increases in the Paleolithic toolkit richness and diversity. During the later Paleolithic, technologies increase not only in terms of diversity but also in their complexity and interdependence. As Mesoudi and O'Brien (2008) have shown, selection broadly favors social learning of information that is hierarchical and structured, and multiple studies have demonstrated that teaching within a social learning environment can increase fitness. We believe that teaching also provides the scaffolding for transmission of more complex cultural traits. Here, we introduce an extension of the Axelrod (1997} model of cultural differentiation in which traits have prerequisite relationships, and where social learning is dependent upon the ordering of those prerequisites. We examine the resulting structure of cultural repertoires as learning environments range from largely unstructured imitation, to structured teaching of necessary prerequisites, and we find that in combination with individual learning and innovation, high probabilities of teaching prerequisites leads to richer cultural repertoires. Our results point to ways in which we can build more comprehensive explanations of the archaeological record of the Paleolithic as well as other cases of technological change.Comment: 24 pages, 7 figures. Submitted to "Learning Strategies and Cultural Evolution during the Paleolithic", edited by Kenichi Aoki and Alex Mesoudi, and presented at the 79th Annual Meeting of the Society for American Archaeology, Austin TX. Revised 5/14/1

The SPHERE view of three interacting twin disc systems in polarized light

Dense stellar environments as hosts of ongoing star formation increase the probability of gravitational encounters among stellar systems during the early stages of evolution. Stellar interaction may occur through non-recurring, hyperbolic, or parabolic passages (a so-called 'fly-by'), through secular binary evolution, or through binary capture. In all three scenarios, the strong gravitational perturbation is expected to manifest itself in the disc structures around the individual stars. Here, we present near-infrared polarized light observations that were taken with the SPHERE/IRDIS instrument of three known interacting twin-disc systems: AS 205, EM∗ SR 24, and FU Orionis. The scattered light exposes spirals likely caused by the gravitational interaction. On a larger scale, we observe connecting filaments between the stars. We analyse their very complex polarized intensity and put particular attention to the presence of multiple light sources in these systems. The local angle of linear polarization indicates the source whose light dominates the scattering process from the bridging region between the two stars. Further, we show that the polarized intensity from scattering with multiple relevant light sources results from an incoherent summation of the individuals' contribution. This can produce nulls of polarized intensity in an image, as potentially observed in AS 205. We discuss the geometry and content of the systems by comparing the polarized light observations with other data at similar resolution, namely with ALMA continuum and gas emission. Collective observational data can constrain the systems' geometry and stellar trajectories, with the important potential to differentiate between dynamical scenarios of stellar interaction